scholarly journals Bacillus NP5 Improves Growth Performance and Resistance Against Infectious Myonecrosis Virus in White Shrimp (Litopenaeus vannamei) (Bacillus NP5 Meningkatkan Pertumbuhan dan Ketahanan Terhadap Infeksi Virus Myonecrosis pada Udang Putih (L. vannamei)

2015 ◽  
Vol 19 (4) ◽  
pp. 211 ◽  
Author(s):  
Widanarni Widanarni ◽  
Munti Yuhana ◽  
Arief Muhammad
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Positive Control ◽  
White Shrimp ◽  
Feed Conversion ◽  
Daily Growth ◽  
Infectious Myonecrosis Virus ◽  
Total Hemocyte Count ◽  
Hemocyte Count

Infectious Myonecrosis (IMN) merupakan salah satu penyakit yang sering menyerang udang vaname. Probiotik banyak digunakan pada budidaya udang karena terbukti mampu mengurangi serangan penyakit pada udang. Penelitian ini bertujuan untuk menguji pengaruh pemberian probiotik Bacillus NP5 melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi udang vaname terhadap infeksi Infectious Myonecrosis Virus (IMNV). Udang vaname Litopenaeus vannamei (2.41±0.07 g ekor-1) diberi pakan yang disuplementasi probiotik Bacillus NP5 dengan dosis yang berbeda, 102 CFU.g-1 (A), 104 CFU.g-1 (B), 106 CFU.g-1 (C), dan kontrol tanpa suplementasi probiotik (kontrol negatif, KN; kontrol positif, KP) selama 30 hari dan dengan tiga ulangan untuk masing-masing dosis, kemudian KP, perlakuan A, B, dan C diuji tantang secara intramuskular dengan IMNV (100 µl.ekor-1). Hasil penelitian ini menunjukkan bahwa udang vaname yang diberi pakan dengan suplementasi probiotik mempunyai laju pertumbuhan harian (LPH), rasio konversi pakan (RKP), dan respons imun yang lebih tinggi. Udang tersebut juga mempunyai total hemocyte count (THC) dan resistensi terhadap IMNV yang lebih tinggi dibandingkan kontrol positif. Konsentrasi probiotik 106 CFU.g-1 memberikan hasil terbaik dalam meningkatkan pertumbuhan, respon imun, dan resistensi udang vaname terhadap infeksi IMNV. Kata kunci: probiotik, Bacillus NP5, Litopenaeus vannamei, pertumbuhan, IMNV Infectious Myonecrosis (IMN) is one of the most prevalent white shrimp diseases. Probiotics are widely used in shrimp cultivation because they have been proven to reduce shrimp disease outbreak. This study aimed to observe the effect of oraly administered probiotic Bacillus NP5 on the white shrimp's growth performance, immune response, and resistance to Infectious Myonecrosis Virus (IMNV) infection. White shrimp Litopenaeus vannamei (2.41±0.07 g individual-1) were fed with a feed supplemented with different doses of the probiotic Bacillus NP5, i.e. 102 CFU.g-1 (A), 104 CFU.g-1 (B), 106 CFU.g-1 (C), and control without any probiotic (negative control, KN; positive control, KP) for 30 days and with three replications for each dose, then KP, treatment A, B, and C were challenged intramuscularly with IMNV (100 µl.shrimp-1). The results of the study showed that white shrimp fed with the supplemented probiotic had higher Daily Growth Rate (DGR), Feed Conversion Ratio (FCR), and immune response. They also had the higher Total Hemocyte Count (THC) and resistance to IMNV than the positive control. Probiotic with concentration of 106 CFU.g-1 gave the highest value on enhancing growth, immunity, and resistance of white shrimp towards IMNV infection. Key words: probiotic, Bacillus NP5, Litopenaeus vannamei, growth, IMNV

scholarly journals Effects of spray-dried animal plasma on growth performance, survival, feed utilization, immune responses, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei)

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257792
Author(s):  
Niti Chuchird ◽  
Tirawat Rairat ◽  
Arunothai Keetanon ◽  
Putsucha Phansawat ◽  
Chi-Chung Chou ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Positive Control ◽  
Feed Utilization ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Total Hemocyte Count ◽  
Spray Dried

Spray-dried animal plasma (SDP) in feed for several animal species provides health benefits, but research about use of SDP in shrimp feed is very limited. The objectives of the present study were to investigate the effects of dietary SDP on growth performance, feed utilization, immune responses, and prevention of Vibrio parahaemolyticus infection in Pacific white shrimp (Litopenaeus vannamei). In Experiment 1, the post-larvae were divided into five groups (four tank/group and 80 shrimp/tank) and fed four times daily diets with porcine SDP at 0, 1.5, 3, 4.5, and 6% of the diet for 45 days. In Experiment 2, the surviving shrimp from Experiment 1 were redistributed into six groups: four SDP groups as in Experiment 1 plus the positive and negative controls (four tank/group and 30 shrimp/tank). They were then challenged with V. parahaemolyticus by immersion at 105 colony-forming units (CFU)/mL and were fed with the same diets for another 4 days. In Experiment 1, shrimp fed 4.5% or 6% SDP diets had significantly higher body weight, survival rate, and improved feed conversion ratio. The immune parameters (total hemocyte count and phagocytic, phenoloxidase, and superoxide dismutase activities) of the shrimp fed 3–6% SDP diets also showed significant enhancement compared to the control. In Experiment 2, the survival rates of the 3–6% SDP groups were significantly higher than the positive control at day 4 after the immersion challenge. Likewise, the histopathological study revealed milder signs of bacterial infection in the hepatopancreas of the 3–6% SDP groups compared to the challenged positive control and 1.5% SDP groups. In conclusion, shrimp fed diets with SDP, especially at 4.5–6% of the diet, showed significant improvement in overall health conditions and better resistance to V. parahaemolyticus infection.

scholarly journals Prebiotic, probiotic, and synbiotic to control Vibrio harveyi and IMNV co-infection in Litopenaeus vannamei

2015 ◽  
Vol 13 (1) ◽  
pp. 11 ◽  
Author(s):  
, Widanarni ◽  
Jeanni Indah Noermala ◽  
, Sukenda
Keyword(s):  
Immune Response ◽  
Litopenaeus Vannamei ◽  
Vibrio Harveyi ◽  
Positive Control ◽  
White Shrimp ◽  
Infectious Myonecrosis Virus ◽  
Probiotic Treatment

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph" align="center"><strong> </strong></p><p class="Pa2">This study aimed to examine the effects of prebiotic, probiotic, and synbiotic in survival and immune response of white shrimp against co-infection of Vibrio harveyi and infectious myonecrosis virus (IMNV). The shrimps used had a body weigth of 2.04±0.20 g/individual, the shrimps were reared at a density of 20 shrimps in 60x30x35 cm3 sized aquarium. The study was conducted with five treatments consisting K(+) (without the addition of prebiotic, probiotic, and synbiotic with co-infection), K(-) (without the addition of prebiotic, probiotic and synbiotic, and without co-infection), P1 (the addition of prebiotic with co-infection), P2 (the addition of probiotic with co-infection), and P3 (the addition of synbiotic with co-infection). The results showed that the addition of prebiotic, probiotic and synbiotic could increase survival and immune response of white shrimp towards co-infection of Vibrio harveyi and IMNV. The best survival was obtained in probiotic treatment (79.17%), followed by prebiotic treatment (75%), synbiotic treatment (70.83%), while the positive control was only 50%.</p><p class="Default"> </p><p class="Pa1">Keywords: white shrimp, prebiotic, probiotic, synbiotic, IMNV, Vibrio harveyi</p><br /><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"><strong> </strong></p><p>Penelitian ini bertujuan untuk menguji pengaruh pemberian prebiotik, probiotik, dan sinbiotik terhadap sintasan dan respons imun udang vaname dengan ko-infeksi <em>Vibrio harveyi </em>dan IMNV (<em>i</em><em>nfectious myonecrosis virus</em>). Udang yang digunakan memiliki bobot 2,04±0,20 g/ekor, dipelihara sebanyak 20 ekor dalam akuarium berukuran 60x30x35 cm3. Penelitian dilakukan dengan lima perlakuan yaitu K(+) (tanpa pemberian prebiotik, probiotik, dan sinbiotik dengan ko-infeksi), K(-) (tanpa pemberian prebiotik, probiotik dan sinbiotik tanpa ko-infeksi), P1 (pemberian prebiotik dengan ko-infeksi), P2 (pemberian probiotik dengan ko-infeksi), dan P3 (pemberian sinbiotik dengan ko-infeksi). Hasil penelitian menunjukkan bahwa pemberian prebiotik, probiotik, dan sinbiotik mampu meningkatkan sintasan dan respons imun udang vaname terhadap ko-infeksi <em>V. harveyi </em>dan IMNV. Sintasan terbaik diperoleh pada perlakuan probiotik sebesar 79,17%, diikuti perlakuan prebiotik sebesar 75%, perlakuan sinbiotik sebesar 70,83%, sedangkan pada kontrol positif hanya mencapai 50%.</p><p> </p><p class="Pa2">Kata kunci: udang vaname, prebiotik, probiotik, sinbiotik, IMNV, <em>Vibrio harveyi</em></p>

scholarly journals Respon imun nonspesifik dan pertumbuhan udang vaname (Litopenaeus vannamei) yang diberi pakan yang ditambahkan nukleotida dengan lama pemberian berbeda

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Oral Administration ◽  
Litopenaeus Vannamei ◽  
Research Result ◽  
Basal Diet ◽  
Experiment Research ◽  
Total Hemocyte Count ◽  
Hemocyte Count ◽  
Nonspecific Immune Response ◽  
Feeding Protocol

Abstract This study evaluated the effects of feeding protocol of nucleotide-supplemented diet on total hemocytes, PO activity and growth of whiteleg shrimp.  Shrimp juveniles were reared in five 120-l glass aquaria at the density of 25 juveniles each.  Shrimp pellet was supplemented with nucleotides mixture at 400 mg.kg-1 diet. Shrimps were fed three time a day at 3%/bw/d.  Feeding protocol was 7 days nucleotides diet – 7 days basal diet alterably for 49 days.  Total hemocyte count, PO activity, and growth were measured at the end of experiment.  Research result showed that oral administration of nucleotides at seven days interval did not affect THC, PO activity, and growth of shrimp. But if the feed was administered successively for four weeks, supplementation of nucleotides would significantly enhance the nonspecific immune response and shrimp growth.   Keywords: Litopenaeus vannamei, nucleotides, PO activity, total hemocyte count

scholarly journals APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei))

2016 ◽  
Vol 10 (2) ◽  
pp. 121-127
Author(s):  
Widanarni Widanarni ◽  
Sukenda Sukenda ◽  
Ghita Ryan Septiani
Keyword(s):  
Survival Rate ◽  
Immune Responses ◽  
Litopenaeus Vannamei ◽  
Ipomoea Batatas ◽  
Challenge Test ◽  
Positive Control ◽  
Negative Control ◽  
White Shrimp ◽  
Infectious Myonecrosis Virus ◽  
Synbiotic Supplementation

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.493±0.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.93±0.025-6.97±0.019% and had higher values than controls (A and B) (P<0.05). Meanwhile, feed conversion value in C and D (1.54±0.142 and 1.58±0.117) were lower than controls (P<0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P<0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.

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