scholarly journals IMPROVEMENT OF BIOETHANOL PRODUCTION BY USING Saccharomyces cerevisiae [Meyen ex E.C. Hansen] IMMOBILIZED ON PRETREATED SUGARCANE BAGASSE

REAKTOR ◽  
2018 ◽  
Vol 18 (03) ◽  
pp. 127 ◽  
Author(s):  
Sita Heris Anita ◽  
Wibowo Mangunwardoyo ◽  
Yopi Yopi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Physical Properties ◽  
Sugarcane Bagasse ◽  
Immobilized Cells ◽  
Ethanol Yield ◽  
Steam Treatment ◽  
Bioethanol Production ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Pretreated Sugarcane Bagasse

Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure.  The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse

scholarly journals Food Grade Ethanol Production Process of Sorghum Stem Juice Using Immobilized Cells Technique

2015 ◽  
Vol 9 (7) ◽  
pp. 8 ◽  
Author(s):  
Tri Widjaja ◽  
Ali Altway ◽  
Arief Widjaja ◽  
Umi Rofiqah ◽  
Rr Whiny Hardiyati Erlian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Sieve ◽  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Ethanol Yield ◽  
Continuous Fermentation ◽  
Continuous Process ◽  
Pichia Stipitis ◽  
Batch Process ◽  
Food Grade

One form of economic development efforts for waste utilization in rural communities is to utilize stem sorghum to produce food grade ethanol. Sorghum stem juice with 150 g/L of sugar concentration was fermented using conventional batch process and cell immobilization continuous process with K-carrageenan as a supporting matrix. The microorganism used was Mutated Zymomonas Mobilis to be compared with a mixture of Saccharomyces Cerevisiae and Pichia Stipitis, and a mixture of Mutated Zymomonas Mobilis and Pichia Stipitis. Ethanol in the broth, result of fermentation process, was separated in packed distillation column. Distilate of the column, still contain water and other impurities, was flown into molecular sieve for dehydration and activated carbon adsorption column to remove the other impurities to meet food grade ethanol specification. The packing used in distillation process was steel wool. For batch fermentation, the fermentation using a combination of Saccharomyces Cerevisiae and Pichia Stipitis produced the best ethanol with 12.07% of concentration, where the yield and the productivity were 63.49%, and 1.06 g/L.h, respectively. And for continuous fermentation, the best ethanol with 9.02% of concentration, where the yield and the productivity were 47.42% and 174.27 g/L.h, respectively, is obtained from fermentation using a combination of Saccharomyces Cerevisiae and Pichia Stipitis also. Fermentation using combination microorganism of Saccharomyces Cerevisiae and Pichia Stipitis produced higher concentration of ethanol, yield, and productivity than other microorganisms. Distillation, molecular sieve dehydration and adsorption process is quite successful in generating sufficient levels of ethanol with relatively low amount of impurities.

Bioethanol production from alkaline-pretreated sugarcane bagasse by consolidated bioprocessing using Phlebia sp. MG-60

2014 ◽  
Vol 88 ◽  
pp. 62-68 ◽  
Author(s):  
Le Duy Khuong ◽  
Ryuichiro Kondo ◽  
Rizalinda De Leon ◽  
To Kim Anh ◽  
Kuniyoshi Shimizu ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Consolidated Bioprocessing ◽  
Bioethanol Production ◽  
Pretreated Sugarcane Bagasse

scholarly journals Efficient Bioethanol Production by a Recombinant Flocculent Saccharomyces cerevisiae Strain with a Genome-Integrated NADP+-Dependent Xylitol Dehydrogenase Gene

2009 ◽  
Vol 75 (11) ◽  
pp. 3818-3822 ◽  
Author(s):  
Akinori Matsushika ◽  
Hiroyuki Inoue ◽  
Seiya Watanabe ◽  
Tsutomu Kodaki ◽  
Keisuke Makino ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Yield ◽  
Xylitol Dehydrogenase ◽  
Wood Chips ◽  
Bioethanol Production ◽  
Xylose Consumption ◽  
Acid Hydrolysate ◽  
A Genome ◽  
Dehydrogenase Gene ◽  
Flocculent Yeast

ABSTRACT The recombinant industrial Saccharomyces cerevisiae strain MA-R5 was engineered to express NADP+-dependent xylitol dehydrogenase using the flocculent yeast strain IR-2, which has high xylulose-fermenting ability, and both xylose consumption and ethanol production remarkably increased. Furthermore, the MA-R5 strain produced the highest ethanol yield (0.48 g/g) from nonsulfuric acid hydrolysate of wood chips.

scholarly journals Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst

2011 ◽  
Vol 91 (4) ◽  
pp. 1001-1006 ◽  
Author(s):  
Hideyo Yoshida ◽  
Kiyotaka Y. Hara ◽  
Kentaro Kiriyama ◽  
Hideki Nakayama ◽  
Fumiyoshi Okazaki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Engineered Saccharomyces Cerevisiae
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