The mutagenic effect of hydroxylamine hydrochloride on the agronomic traits of Sesame (Sesamum indicum L.)

Deciphering the genetic basis of quantitative agronomic traits is a prerequisite for their improvement. Herein, we identified loci governing the main sesame lignans, sesamin and sesamolin variation in a recombinant inbred lines (RILs, F8) population under two environments. The content of the two lignans in the seeds was investigated by HPLC. The sesamin and sesamolin contents ranged from 0.33 to 7.52 mg/g and 0.36 to 2.70 mg/g, respectively. In total, we revealed 26 QTLs on a linkage map comprising 424 SSR markers, including 16 and 10 loci associated with sesamin and sesamolin variation, respectively. Among them, qSmin_11.1 and qSmol_11.1 detected in both the two environments explained 67.69% and 46.05% of the phenotypic variation of sesamin and sesamolin, respectively. Notably, qSmin11-1 and qSmol11-1 were located in the same interval of 127-127.21cM on LG11 between markers ZMM1776 and ZM918 and acted as a pleiotropic locus. Furthermore, two potential candidate genes (SIN_1005755 and SIN_1005756) at the same locus were identified based on comparative transcriptome analysis. Our results suggest the existence of a single gene of large effect that controls expression, both of sesamin and sesamolin, and provide genetic information for further investigation of the regulation of lignan biosynthesis in sesame.

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Effects of Hydrogen Peroxide and Benzene Treatments on Morphological Traits of Sesame (Sesamum indicum L.)

10.47363/jftns/2021(3)126 ◽

2021 ◽

pp. 1-9

Author(s):

Ablaku AB ◽

◽

Ablaku BE ◽

Keyword(s):

Hydrogen Peroxide ◽

Genetic Variability ◽

Plant Height ◽

Morphological Traits ◽

Agronomic Traits ◽

Mutation Breeding ◽

Sesamum Indicum ◽

Chemical Mutagens ◽

Number Of Leaves ◽

Effective Mutagen

A study was carried out on the effects of hydrogen peroxide and benzene treatments on morphological traits of sesame (Sesamum indicum L.) in Keffi. NCRIBEN-02M variety of sesame was exposed to varying concentration (100, 75, 50, 25 and 0%), of benzene, hydrogen peroxide, and the mixture of the two chemical mutagens. The M2 generation of the genotypes were assessed for plant height, number of leaves, leaves area. Benzene treatment, at all levels, have the highest plant height. The increase in number of leaves and leaf area are significant at p≤0.05 and 95% confidence. The optimum concentrations of the mutagens used for mutation in sesame, were effective at 100 and 75% of all the treatments. The most effective mutagen for inducing mutation in sesame under Keffi environment is benzene, followed by Hydrogen Peroxide. Benzene and hydrogen peroxide have proven themselves as chemical mutagens, in mutation breeding. They have unlocked several agronomic traits in sesame. Further, study on these mutagens will enhance the genetic variability in the growing of sesame for higher performance

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Field Management of Cercospora Leaf Spot induced by Cercospora sesami Zimm. Using Plant Extracts And A Synthetic Fungicide As A Method Of Reducing The Effects On Agronomic Traits Associated With Yield Of Sesame (Sesamum indicum L.)

IOSR Journal of Agriculture and Veterinary Science ◽

10.9790/2380-0142328 ◽

2012 ◽

Vol 1 (4) ◽

pp. 23-28

Author(s):

Nahunnaro H.

Keyword(s):

Plant Extracts ◽

Leaf Spot ◽

Agronomic Traits ◽

Sesamum Indicum ◽

Cercospora Leaf Spot ◽

Field Management

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Deformation of Yeast Plasma Membrane by Ethidium Bromide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103334 ◽

1988 ◽

Vol 46 ◽

pp. 254-255

Author(s):

E. Keyhani

Keyword(s):

Plasma Membrane ◽

Thin Section ◽

Ethidium Bromide ◽

Freeze Fracture ◽

Mutagenic Effect ◽

Yeast Cells ◽

Hydrophobic Region ◽

Thin Section Electron Microscopy ◽

Section Electron ◽

Deep Pits

The mutagenic effect of ethidium bromide on the mitochondrial DNA is well established. Using thin section electron microscopy, it was shown that when yeast cells were grown in the presence of ethidium bromide, besides alterations in the mitochondria, the plasma membrane also showed alterations consisting of 75 to 110 nm-deep pits. Furthermore, ethidium bromide induced an increase in the length and number of endoplasmic reticulum and in the number of intracytoplasmic vesicles.Freeze-fracture, by splitting the hydrophobic region of the membrane, allows the visualization of the surface view of the membrane, and consequently, any alteration induced by ethidium bromide on the membrane can be better examined by this method than by the thin section method.Yeast cells, Candida utilis. were grown in the presence of 35 μM ethidium bromide. Cells were harvested and freeze-fractured according to the procedure previously described.

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