scholarly journals IMPACT OF OOCYTE SIZE ON LHRHa INDUCED OVULATION AND FERTILIZED EGG QUALITY IN SADDLED BREAM OBLADA MELANURA (LINNAEUS, 1758)

2013 ◽  
Vol 71 (2) ◽  
pp. 80-83
Author(s):  
Nenad Antolovi� ◽  
Valter Ko�ul ◽  
Nik�a Glavi� ◽  
Jak�a Bolotin
Keyword(s):  
Egg Quality ◽  
Oocyte Size ◽  
Induced Ovulation ◽  
Fertilized Egg

GnRHa-induced ovulation of brown trout (Salmo trutta) and its effects on egg quality

Aquaculture ◽  
1992 ◽  
Vol 106 (3-4) ◽  
pp. 379-392 ◽  
Author(s):  
Costadinos C. Mylonas ◽  
Jeffrey M. Hinshaw ◽  
Craig V. Sullivan
Keyword(s):  
Brown Trout ◽  
Salmo Trutta ◽  
Egg Quality ◽  
Induced Ovulation ◽  
Brown Trout Salmo Trutta

Immunoprobe localization in mammalian embryos

1990 ◽  
Vol 48 (3) ◽  
pp. 32-33
Author(s):  
Patricia G. Calarco ◽  
Margaret C. Siebert
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Thin Sections ◽  
Relative Lack ◽  
Large Size ◽  
Mammalian Embryos ◽  
Antigen Localization ◽  
Difficult Challenge ◽  
Lowicryl K4m ◽  
Fertilized Egg

Visualization of preimplantation mammalian embryos by electron microscopy is difficult due to the large size of the ircells, their relative lack of internal structure, and their highly hydrated cytoplasm. For example, the fertilized egg of the mouse is a single cell of approximately 75μ in diameter with little organized cytoskelet on and apaucity ofor ganelles such as endoplasmic reticulum (ER) and Golgi material. Thus, techniques that work well on tissues or cell lines are often not adaptable to embryos at either the LM or EM level.Over several years we have perfected techniques for visualization of mammalian embryos by LM and TEM, SEM and for the pre-embedding localization of antigens. Post-embedding antigenlocalization in thin sections of mouse oocytes and embryos has presented a more difficult challenge and has been explored in LR White, LR Gold, soft EPON (after etching of sections), and Lowicryl K4M. To date, antigen localization has only been achieved in Lowicryl-embedded material, although even with polymerization at-40°C, the small ER vesicles characteristic of embryos are unrecognizable.

Japon Bıldırcını Rasyonlarında Soya Küspesi Yerine Pamuk Tohumu Küspesi İkamesinin Yumurta Verim ve Kalite Özelliklerine Etkisi

2013 ◽  
Vol 1 (1) ◽  
pp. 44 ◽  
Author(s):  
Arda Yıldırım ◽  
Ergin Öztürk
Keyword(s):  
Protein Content ◽  
Cottonseed Meal ◽  
Soybean Meal ◽  
Shell Thickness ◽  
Egg Production ◽  
Egg Quality ◽  
Yield Traits

This study was conducted to determine the effect of cottonseed meal (CSM) incorporated into laying rations in place of soybean meal (SBM) at different ratios on yield traits. The birds began to lay at 6th week, 180 female and 45 male quails were used in laying period experiment. Birds were fed with rations containing 20% CP and 3000 Kcal/kg ME up to 20-week age (Laying period). CSM as a substitute, five different rations of the protein content (0, 30, 58, 86 and 100%) for SBM to basal diets based on corn-soybean meal were used. The results showed that there were no differences in terms of egg yield traits, cumulative feed consumptions and viabilities during the laying period. The highest dry shell rate and shell thickness were obtained from 5th group and 1st group, respectively. As a result, adding CSM instead of SBM in laying period were no significantly differences in terms of egg production and egg quality in laying period.

МОДЕЛИРОВАНИЕ СИСТЕМ ЭКСТРАКОРПОРАЛЬНОГО СОЗРЕВАНИЯ ООЦИТОВ КРУПНОГО РОГАТОГО СКОТА С ИСПОЛЬЗОВАНИЕМ СТРУКТУРНЫХ КОМПОНЕНТОВ ОВАРИАЛЬНЫХ ФОЛЛИКУЛОВ *

2019 ◽  
pp. 20-22
Author(s):  
T.I. KUZMINA ◽  
I.V. CHISTYAKOVA
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Bovine Serum ◽  
Egg Quality ◽  
Fetal Bovine Serum ◽  
Reproductive Technologies ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
Cultural Systems ◽  
Fetal Bovine

Создание эффективной унифицированной системы дозревания донорских ооцитов обеспечит повышение результативности инновационных клеточных репродуктивных технологий. В исследовании проведен сравнительный мониторинг показателеймейотического созревания ооцитов коров, созревших в различных системах, дополненных структурными компонентами фолликулов (СКФ стенки фолликулов, клетки гранулезы, белки) и фолликулярной жидкостью,а также потенций к развитию из них доимплантационных эмбрионов. Анализу подверглись ооциты, прокультивированные в следующих системах:среда ТС199 с добавлением 10 фетальной бычьей сыворотки (ФБС), 50 мкг/мл эстрадиола, 10 мкг/мл лютеинизирующего гормона (ЛГ), 10 мкг/мл фолликулостимулирующего гормона (ФСГ) среда ТС199 с 10 эстральной сывороткой коров среда ТС199 с 50 жидкости из фолликулов диаметром 9 мм среда ТС199 с добавлением белков фолликулярной жидкости молекулярной массой 65 кДасреда ТС199 с 10 ФБС и 1106 клеток гранулезы среда ТС199 с 10 ФБС и тканью фолликула. В культуральные среды ко всем исследованным группам ооцитов добавляли антибиотики. Использование CКФ обеспечило значительное снижение доли ооцитов с дегенерированным хроматином, что способствовало увеличению уровня доимпланационных эмбрионов на стадии бластоцисты. Так, доля бластоцист, развившихся из ооцитов, созревших в среде со стенками фолликулов,составила43,5. В этой же группе выявлен минимальный уровень дегенерированных зародышей (6,45). Полученные данные предлагается использовать при моделировании систем дозревания ооцитов коров с целью повышения качества яйцеклеток.The creation of an effective unified maturation system of donor oocytes provides an increase in the efficiency of innovative cellular reproductive technologies. The comparative analysis of the meiotic maturation indicators of bovine oocytes, which were matured in different cultural systems modified by follicular structural components (FSC follicular walls, granulosa cells, proteins) and follicular fluid, as well as the potential for preimplantation embryonic development were evaluated in this study. Oocytes matured in following cultural systems: medium TC199 supplemented with 10 fetal bovine serum and 50 g/ml of estradiol, 10 g/ml of luteinizing hormone (LH), 10 g/ml of folliclestimulating hormone (FSH) medium TC199 with 10 estrous cow serum medium TC199 with 50 liquid from follicles with a diameter of 9 mm medium TC199 supplemented with the follicular fluid proteins with molecular weight 65 kDa medium TC199 with 10 fetal bovine serum and 1106 granulosa cells medium TC199 with the addition of 10 fetal bovine serum and follicle tissues were analyzed. Antibiotics were added to cultural media of all experimental groups of oocytes. The usage of FSC ensured the decrease in the proportion of oocytes with degenerated chromatin, which contribute the rise of the level of preimplantation embryos at the blastocyst stage. Thus, the proportion of blastocysts developed from oocytes matured in medium supplemented with follicular walls was 43.5. In the same experimental group, the number of degenerated embryos was 6.45. The obtained data are supposed to be used for modeling the cultural systems of cow oocytes in order to improve the egg quality.

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