5. GEL ISOELECTRIC FOCUSING

A range of laboratory methods was examined for their ability to distinguish between 19 barley cultivars currently grown in Australia. Aleurone colour, revealed after mechanical or chemical dehulling, differentiated Abyssinian, Atlas, Cape and Corvette from the other cultivars. Peroxidase and phenol testing were not useful. Seven different patterns were obtained for the hordeins of lowest mobility by starch gel electrophoresis. Further distinction was provided by flat gel isoelectric focusing of the water-soluble and hordein proteins for which 13 different pattern-groupings were obtained. The two electrophoretic techniques complemented one another, so that the use of both methods left only a few cultivars that could not be distinguished.

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ACIDIC PEROXIDASES OF APPLE ROOTSTOCKS

Canadian Journal of Plant Science ◽

10.4141/cjps87-042 ◽

1987 ◽

Vol 67 (1) ◽

pp. 293-297 ◽

Cited By ~ 1

Author(s):

DAVID L. WELLER

Keyword(s):

Molecular Weight ◽

Isoelectric Focusing ◽

Peroxidase Activity ◽

Sucrose Gradient ◽

Bark Extract ◽

Root Extract ◽

Apple Rootstock ◽

Apple Rootstocks ◽

Gel Isoelectric Focusing ◽

Isoenzyme Patterns

The peroxidase activity in extracts of shoot bark from Michigan State Apple Clone (MAC) 24 and in extracts of roots of MAC 24, East Mailing Long Ashton (EMLA) 27, and Oregon Apple Rootstock (OAR) 1 were characterized as being primarily acidic by broad range sucrose gradient isoelectric focusing. Gel isoelectric focusing (GIEF) using a narrower conventional or immobilized pH gradient showed that this activity could be separated into about a dozen isoenzymes. The GIEF isoenzyme patterns of MAC 24 shoot bark extract peroxidase and root extract peroxidases of MAC 24, EMLA 27, and OAR 1 differed. Similar values were obtained for the molecular weight of the peroxidase activity of these extracts. The results indicate that the acidic isoenzymes of apple peroxidase are of similar size.Key words: Apple, rootstocks (apple), peroxidase, molecular weight, isoenzymes

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Gel isoelectric focusing of wheat alcohol dehydrogenase

Theoretical and Applied Genetics ◽

10.1007/bf00265706 ◽

1979 ◽

Vol 54 (1) ◽

pp. 37-39 ◽

Cited By ~ 10

Author(s):

O. Fejér ◽

K. Orosz-Fejér ◽

A. Belea

Keyword(s):

Alcohol Dehydrogenase ◽

Isoelectric Focusing ◽

Gel Isoelectric Focusing

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Rapid Fish Species Identification by Agarose Gel Isoelectric Focusing of Sarcoplasmic Proteins

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/64.1.38 ◽

1981 ◽

Vol 64 (1) ◽

pp. 38-43

Author(s):

Ronald C Lundstrom

Keyword(s):

Species Identification ◽

Isoelectric Focusing ◽

Fish Species ◽

Protein Pattern ◽

Agarose Gel ◽

Tissue Fluid ◽

Protein Patterns ◽

Fish Species Identification ◽

Species Specific ◽

Gel Isoelectric Focusing

Abstract A rapid method is described for fish species identification by agarose gel isoelectric focusing (AGIEF). The AGIEF method can be completed in less than 2 h and gives reproducible species-specific sarcoplasmic protein patterns. Protein patterns are similar using either centrifuged tissue fluid or muscle tissue as the sample. One species, monkfish (Lophius americanus), has a polymorphic protein pattern. A predominant pattern was found in 66.7% of the individuals; 2 variant patterns were equally distributed among the remaining 33.3%. AGIEF offers a more rapid, less expensive alternative to the current AOAC official first action method for fish species identification based on polyacrylamide gel isoelectric focusing.

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