Identification of Australian barley cultivars by laboratory methods: gel electrophoresis and gel isoelectric focusing of the endosperm proteins

1977 ◽  
Vol 17 (89) ◽  
pp. 1020 ◽  
Author(s):  
J McCausland ◽  
CW Wrigley
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Water Soluble ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Laboratory Methods ◽  
Barley Cultivars ◽  
Starch Gel ◽  
Electrophoretic Techniques ◽  
Gel Isoelectric Focusing

A range of laboratory methods was examined for their ability to distinguish between 19 barley cultivars currently grown in Australia. Aleurone colour, revealed after mechanical or chemical dehulling, differentiated Abyssinian, Atlas, Cape and Corvette from the other cultivars. Peroxidase and phenol testing were not useful. Seven different patterns were obtained for the hordeins of lowest mobility by starch gel electrophoresis. Further distinction was provided by flat gel isoelectric focusing of the water-soluble and hordein proteins for which 13 different pattern-groupings were obtained. The two electrophoretic techniques complemented one another, so that the use of both methods left only a few cultivars that could not be distinguished.

STUDIES ON THE LIVETINS OF EGG YOLK: III. HETEROGENEITY AND IMMUNOELECTROPHORETIC BEHAVIOR OF BETA-LIVETIN

1966 ◽  
Vol 44 (10) ◽  
pp. 1357-1364 ◽  
Author(s):  
Shun-Fong Hui ◽  
R. H. Common
Keyword(s):  
Serum Albumin ◽  
Gel Electrophoresis ◽  
Egg Yolk ◽  
The Other ◽  
Positive Staining ◽  
Staining Reaction ◽  
Starch Gel Electrophoresis ◽  
Hen’S Egg ◽  
Starch Gel ◽  
The One

Starch-gel electrophoresis of the total livetins of hen's egg yolk resolved 16 zones: seven major zones, six minor zones, and three faint, diffuse zones. One zone was identified with the major component of paper electrophoretic alpha-livetin and hence with serum albumin. Four of the major zones were identified with the major components of paper electrophoretic beta-livetin on the one hand, and with an electrophoretically heterogeneous livetin antigen (livetin antigen 3) on the other hand, thus establishing the electrophoretic heterogeneity and relative immunological homogeneity of the paper electrophoretic beta-livetin fraction. The other two major starch-gel electrophoretic zones were identified as transferrins by their positive staining reaction for iron and comparison of their mobilities with two corresponding serum starch-gel fractions.

INFLUENCE OF ALIEN GENOME COMBINATIONS ON PROTEIN SYNTHESIS IN CEREALS

1964 ◽  
Vol 42 (12) ◽  
pp. 1647-1657 ◽  
Author(s):  
F. C. Yong ◽  
A. M. Unrau
Keyword(s):  
Protein Synthesis ◽  
Gel Electrophoresis ◽  
Triticum Durum ◽  
Tetraploid Wheat ◽  
Water Soluble ◽  
Starch Gel Electrophoresis ◽  
Triticum Vulgare ◽  
Qualitative And Quantitative ◽  
Starch Gel ◽  
Variable Influence

Starch-gel electrophoresis of the water-soluble, salt-soluble, and alcohol soluble proteins of Triticale, Triticum durum, Secale cereale, Triticum vulgare, and Tritipyron revealed both qualitative and quantitative differences. The experimental evidence obtained indicated that the biosynthetic potential of the alien genomes in the synthetic species (Triticale) was not fully maintained. A variable influence of the tetraploid wheat (T. durum) genomes on protein synthesis in the three hexaploid cereals (Triticale, T. vulgare, and Tritipyron) was observed.

A Genetic Comparison of Sympatric Populations of Sand Lance (Genus Ammodytes) from the Region East of Cape Soya, Japan

1989 ◽  
Vol 46 (11) ◽  
pp. 1945-1951 ◽  
Author(s):  
Hiroaki Okamoto
Keyword(s):  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Distinct Species ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Sympatric Populations ◽  
Sand Lance ◽  
Protein Coding ◽  
Starch Gel ◽  
Genetic Comparison

Sand fance (Genus Ammodytes) collected from four stations off Japan and one station at Kodiak, Alaska were genetically characterized at 17 protein coding loci using starch-gel electrophoresis. Sand lance in Wakkanai (Cape Soya, Japan) consist of two genetically distinct groups. They are fixed for different alleles at four loci (Ldh-2, -3, G3pdh-2, and Mdhp-2). The genetic structure of one of the groups (Wakkanai-a group, W-a) is similar to that of A. personatus around Japan. The other group (Wakkanai-b group, W-b) has different genetic structure from either A. personatus or the Alaskan collection, which is presumed to belong to A. hexapterus. It is not presently possible to identify the affiliation of the W-b group; however, despite its sympatry with the W-a group, it is reproductively isolated and therefore is probably a distinct species occurring northeast of Hokkaido.

Patterns of evolution and migration in the arctic ground squirrel, Spermophilus parryii (Richardson)

1977 ◽  
Vol 55 (4) ◽  
pp. 748-758 ◽  
Author(s):  
Charles F. Nadler ◽  
Robert S. Hoffmann
Keyword(s):  
Body Size ◽  
Gel Electrophoresis ◽  
Ground Squirrel ◽  
The Other ◽  
The Arctic ◽  
Starch Gel Electrophoresis ◽  
Siberian Population ◽  
Spermophilus Parryii ◽  
Starch Gel ◽  
And Migration

Serum transferrins and 11 other genetically controlled proteins representing 17 loci were examined by starch-gel electrophoresis from Siberian, Alaskan, and Canadian populations of Spermophilus parryii. Six transferrin alleles were identified. Arctic populations (S. p. parryii, S. p. osgoodi) were characterized by Tf 6 occurring alone or together with Tf 7 whereas middle and subarctic populations exhibited Tf 7 occurring either alone (S. p. ablusus, S. p. lyratus, S. p. plesius) or together with Tf 5 (S. p. plesius). Tf 8, Tf 9, and Tf 19 constituted local variants. Tf 6 displayed a clinal distribution, increasing in frequency eastward and paralleling a clinal increase in body size. Three PGM2 alleles were observed, the frequencies of which tend to differentiate arctic S. p. parryii from subarctic S. p. ablusus. G6PD-b occurred uniformly in North America and in one Siberian population; a second population (two specimens) exhibited G6PD-a, thereby suggesting that G6PD polymorphism may be present in Siberian S. parryii. The other nine proteins were monomorphic in all Holarctic populations.

A COMPARISON OF HISTONES FROM CHICKEN TISSUES BY ZONE ELECTROPHORESIS IN STARCH GEL

1961 ◽  
Vol 39 (3) ◽  
pp. 485-491 ◽  
Author(s):  
J. M. Neelin ◽  
G. C. Butler
Keyword(s):  
Cell Differentiation ◽  
Ionic Strength ◽  
Gel Electrophoresis ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Electrophoretic Patterns ◽  
Zone Electrophoresis ◽  
Starch Gel ◽  
Chicken Erythrocytes ◽  
Characteristic Zone

Histories were extracted at pH 1.7 from washed nuclei of chicken erythrocytes, spleen, liver, and testis and compared by starch-gel electrophoresis at pH 5.0, ionic strength 0.020. Spleen and liver histories displayed the most complex electrophoretic patterns with 18 zones each and differed only in relative proportions of certain zones. Erythrocyte histone contained a characteristic zone while lacking a group present in spleen and liver histones. Testis histone with only seven zones differed markedly from the other three. These results were consistent with chromatograms of erythrocyte, spleen, and liver histones on sodium IRC-50. The suggested correlation of tissue-specific histones with cell differentiation is discussed.

Genetic diversity in Old Subterranean Clover (Trifolium subterraneum L.) Populations in Western Australia. I. Pastures sown initially to the Dwalganup strain

1988 ◽  
Vol 39 (6) ◽  
pp. 1051 ◽  
Author(s):  
RC Rossiter ◽  
WJ Collins
Keyword(s):  
Gel Electrophoresis ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Morphological Characters ◽  
The Other ◽  
Annual Rainfall ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Spring Leaf

Subterranean clover burrs were collected during summer from 28 sites, 10 of which were in the <500 mm annual rainfall zone. All sites had been sown to the Dwalganup strain, 30-50 years ago. Spaced plants were grown from seed from burr subsamples of each population. Several morphological characters, flowering dates, and oestrogenic isoflavones in late spring leaf samples were determined. At maturity, burr samples were collected from each plant, and the isozyme patterns in seeds were determined for eight enzyme systems using starch gel electrophoresis. Populations from the lower rainfall (< 500 mm) sites usually consisted of one or two strains - Dwalganup with or without Geraldton - and variant (or 'unknown') genotypes were very rare or absent. Populations from the higher rainfall (> 500 mm) sites, on the other hand, comprised about 50% of a few known strains - mostly Dwalganup and/or Dinninup - the remaining 50% consisting of numerous variant genotypes. Infrequent outcrossing between the Dwalganup and Mt Barker strains is the likely major source of this variation. Possible explanations for the lack of variant genotypes in the lower rainfall zone are discussed.

ESTERASE ISOZYMES IN CANADIAN BARLEY CULTIVARS

1972 ◽  
Vol 52 (4) ◽  
pp. 507-516 ◽  
Author(s):  
GEORGE FEDAK ◽  
TIBOR RAJHATHY
Keyword(s):  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Esterase Isozyme ◽  
Esterase Isozymes ◽  
Isozyme Patterns ◽  
Barley Cultivars ◽  
Starch Gel

Fifty-five currently licensed Canadian cultivars and some parental genotypes were analyzed by starch-gel electrophoresis for esterase isozyme patterns and extent of polymorphism for these isozymes. Each cultivar was assigned to one of nine different patterns observed. Fifty-one percent (28) of the cultivars exhibited polymorphic isozyme patterns. The polymorphism was a varietal characteristic and apparently not associated with the age of the cultivar or area of adaptability.

Comparison of the PI patterns of α1--antitrypsin on acid starch gel electrophoresis and isoelectric focusing

1981 ◽  
Vol 41 (1) ◽  
pp. 15-20 ◽  
Author(s):  
R. R. Frants ◽  
G. J. M. Hooghwinkel ◽  
A. W. Eriksson
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Starch Gel

scholarly journals Genetics of plasma transferrins in the mouse

1960 ◽  
Vol 1 (3) ◽  
pp. 431-438 ◽  
Author(s):  
B. L. Cohen
Keyword(s):  
Gel Electrophoresis ◽  
Molecular Basis ◽  
Plasma Proteins ◽  
Inbred Strains ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Widespread Distribution ◽  
Agouti Locus ◽  
Inbred Strains Of Mice ◽  
Starch Gel

1. The plasma proteins of six inbred strains of mice have been studied, using starch-gel electrophoresis.2. The existence of two alternative plasma transferrin (β-globulin) phenotypes has been demonstrated. Five of the strains have one of these and one strain has the other. Each of the two transferrin patterns comprises three (or possibly only two) electrophoretic bands. The two patterns differ in all of these bands.3. The two transferrin types recognized are determined by a pair of allelic, autosomal genes (designated TrfA and TrfB). The TrfA phenotype (CBA strain) is determined by the genotype TrfA/TrfA, and the TrfB phenotype (A, C57BL, JU, KL, RIII strains) by the genotype TrfB/TrfB. The phenotype TrfAB, of the heterozygote (genotype TrfA/TrfB), is distinguishable and shows four (or possibly only three) bands. In this way it closely resembles a mixture of equal parts of TrfA and TrfB plasma.4. No linkage was detected between the Trf locus and sex, the agouti locus or the haemoglobin locus.5. The possible molecular basis of the action of the transferrin alleles in the mouse, and the widespread distribution in mammals of polymorphism involving the transferrins, are discussed.

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