scholarly journals FXYD6 overexpression in HBV-related hepatocellular carcinoma with cirrhosis

2020 ◽  
Vol 15 (1) ◽  
pp. 259-266
Author(s):  
Xiongfei Chen ◽  
Lishuang Ding ◽  
Deshuai Kong ◽  
Xiulei Zhao ◽  
Lili Liao ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Normal Liver ◽  
Early Recurrence ◽  
Tumor Diameter ◽  
Tumor Number ◽  
B Virus ◽  
Expression Rate ◽  
Polymerase Chain ◽  
And Gender ◽  
Liver Tissues

AbstractObjectiveThe aim of this study was to investigate the expression of FXYD domain-containing ion transport regulator 6 (FXYD6) mRNA and protein in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) tissues with cirrhosis, the corresponding paracancerous tissues and the normal liver tissues, and to explore the clinical significance of FXYD6 expression in HBV-related HCC with cirrhosis.MethodsThe FXYD6 mRNA and protein were examined by semi-quantitative reverse transcription polymerase chain reaction and immunohistochemistry, respectively.ResultsThe FXYD6 mRNA in HBV-related HCC tissues was significantly higher than that in the cirrhosis tissues or that in the normal liver tissues. The positive expression rate of FXYD6 protein was statistically higher in HBV-related HCC tissues than that in HBV-related cirrhosis or that in normal liver tissues. There was no significant correlation between the expression of FXYD6 protein and gender, age, histological differentiation, tumor diameter, tumor number, integrity of tumor capsule or not and alpha fetoprotein (AFP) concentration in serum, but the protein expression was associated with microvascular invasion, pathological stage, and early recurrence after operation within 1 year.ConclusionFXYD6 might be involved in hepatocyte carcinogenesis and tumor progression in HBV-related HCC with cirrhosis and indicated a poor prognosis.

scholarly journals Involvement of TRPC7-AS1 Expression in Hepatitis B Virus-Related Hepatocellular Carcinoma

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Shaoliang Zhu ◽  
Hang Ye ◽  
Xiaojie Xu ◽  
Weiru Huang ◽  
Ziyu Peng ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Normal Liver ◽  
Relative Level ◽  
Normal Liver Cell ◽  
B Virus ◽  
Liver Tissues

Objective. To investigate the expression of transient receptor potential (TRP) superfamily genes, especially TRPC7-AS1 in hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC). Methods. Three cancer samples of HBV-related HCC at phase IV and matched paracancerous liver tissues were included in the study. Total RNA was extracted, and differential expression of RNA was screened by high-throughput transcriptome sequencing. The expression of TRPC7-AS1 was detected by quantitative real-time PCR. The N6-adenosyl methylation RNA in MHCC97H, HepG2, and HL-7702 was enriched by coimmunoprecipitation with m6A antibody, and the relative level of N6-adenosyl methylation RNA in TRPC7-AS1 was detected. Results. The expression of TRP family genes in cancer tissues was higher than that in paracancerous liver tissues, including TRPC7-AS1, TRPC4AP, PKD1P6, and PKD1P1. Moreover, the expression level of TRPC7-AS1 in MHCC97H and HepG2 was also significantly higher than that in L02, a normal liver cell. The methylation level of N6-adenosine of TRPC7-AS1 was lower in HepG2 cells than that in L02 cells. Conclusion. TRP superfamily genes, especially TRPC7-AS1, were highly expressed in HBV-related HCC. TRPC7-AS1 could be a potential therapeutic target or diagnostic marker for HCC.

scholarly journals Preoperative Risk Stratification for Early Recurrence of HBV-related Hepatocellular Carcinoma After Deceased Donor Liver Transplantation:A Five-Eight Model Development and Validation

2019 ◽  
Author(s):  
Abdulahad Abdulrab Mohammed Al-Ameri ◽  
Xuyong Wei ◽  
Lidan Lin ◽  
Zhou Shao ◽  
Haijun Guo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Model Development ◽  
Deceased Donor ◽  
Early Recurrence ◽  
Individual Risk ◽  
Tumor Diameter ◽  
Tumor Number ◽  
Serum Alpha Fetoprotein ◽  
Transplant Registry ◽  
Development And Validation

Abstract Background: Early recurrence of hepatocellular carcinoma (HCC) after liver transplantation (LT) is associated with poor surgical outcomes. This study aims to construct a preoperative model to predict individual risk of post-LT HCC recurrence. Methods: Data of 748 adult patients who underwent deceased donor LT for HCC between January 2015, and February 2019 were collected retrospectively from the China Liver Transplant Registry database and randomly divided into training (n=486) and validation(n=262) cohorts. A multivariate analysis was performed and the five-eight model was developed. Results: A total of 748 patients were included in the study; of them, 96% had hepatitis B virus (HBV) and 84 % had cirrhosis. Pre-LT serum alpha-fetoprotein (AFP), tumor number and largest tumor diameter were incorporated to construct the 5-8 model which can stratify patients accurately according to their risk of recurrence into three prognostic subgroups; low-(0-5 points), medium-(6–8 points) and high-risk (>8 points) with 2-year post-LT recurrence rate of (5%,20% and 51%,p<0.001) respectively.The 5-8 model was better than Milan, Hangzhou, and AFP-model for prediction of HCC early recurrence. These findings were confirmed by the results of the validation cohort. Conclusions: The 5-8 model is a simple validated and accurate tool for preoperative stratification of early recurrence of HCC after LT.

scholarly journals Preoperative and Postoperative Nomograms in Predicting Early Recurrence of Hepatocellular Carcinoma Without Macrovascular Invasion After Curative Resection

2021 ◽  
Author(s):  
Yanfang Zhang ◽  
Liangliang Xu ◽  
Mingqing Xu ◽  
Hong Tang
Keyword(s):  
Risk Factors ◽  
Hepatocellular Carcinoma ◽  
Validation Cohort ◽  
Predictive Performance ◽  
Early Recurrence ◽  
Tumor Diameter ◽  
Training Cohort ◽  
Factors Associated ◽  
Tumor Number ◽  
Before And After

Abstract This study aimed to establish pre- and postoperative nomograms in predicting postoperative early recurrence (ER) for hepatocellular carcinoma (HCC) without macrovascular invasion. The patients who underwent curative LR for HCC from January 2012 to December 2016 in our center were divided into training and internal prospective validation cohorts. Nomograms were constructed based on the independent risk factors derived from multivariate logistic regression analyses in training cohort. The predictive performance of nomograms was validated by internal prospective validation cohort. A total of 698 patients fulfilled with eligible criteria. Among them, 265 out of 482 patients (55.0%) in training cohort and 120 out 216 (55.6%) patients in validation cohort developed ER. The preoperative risk factors associated with ER were age, alpha fetoprotein (AFP), tumor diameter, tumor number; the postoperative risk factors associated with ER were age, tumor diameter, tumor number, microvasular invasion (MVI) and differentiation. The pre- and postoperative nomograms based on these factors showed good accuracy with C-indices of 0.712 and 0.850 in training cohort, and 0.754 and 0.857 in validation cohort, respectively. The calibration curves showed optimal agreement between the prediction by the nomograms and actual observation. The area under the receiver operating characteristic curves of pre- and postoperative nomograms were 0.721 and 0.848 in training cohort, and 0.754 and 0.844 in validation cohort, respectively. Present nomograms showed good performance in predicting ER for HCC without macrovascular invasion before and after surgery, which were helpful for doctors in designation of treatments and selection of patients for regularly surveillance or administration of neoadjuvant therapies.

scholarly journals Preoperative Risk Stratification for Early Recurrence of HBV-related Hepatocellular Carcinoma After Deceased Donor Liver Transplantation:A Five-Eight Model Development and Validation

2019 ◽  
Author(s):  
Abdulahad Abdulrab Mohammed Al-Ameri ◽  
Xuyong Wei ◽  
Lidan Lin ◽  
Zhou Shao ◽  
Haijun Guo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Model Development ◽  
Deceased Donor ◽  
Early Recurrence ◽  
Individual Risk ◽  
Tumor Diameter ◽  
Tumor Number ◽  
Serum Alpha Fetoprotein ◽  
Transplant Registry ◽  
Development And Validation

Abstract Background Early recurrence of hepatocellular carcinoma (HCC) after liver transplantation (LT) is associated with poor surgical outcomes. Objectives To construct a preoperative model to predict individual risk of post-LT HCC recurrence. Methods Data of 748 adult patients who underwent deceased donor LT for HCC between January 2015, and February 2019 were collected retrospectively from the China Liver Transplant Registry database and randomly divided into training (n=486) and validation(n=262) cohorts. A multivariate analysis was performed and the five-eight model was developed. Results A total of 748 patients were included in the study; of them, 96% had hepatitis B virus (HBV) and 84 % had cirrhosis. Pre-LT serum alpha-fetoprotein (AFP), tumor number and largest tumor diameter were incorporated to construct the 5-8 model which can stratify patients accurately according to their risk of recurrence into three prognostic subgroups; low-(0-5 points), medium-(6–8 points) and high-risk (>8 points) with 2-year post-LT recurrence rate of (5%,20% and 51%,p<0.001) respectively.The 5-8 model was better than Milan, Hangzhou, and AFP-model for prediction of HCC early recurrence. These findings were confirmed by the results of the validation cohort. Conclusions The 5-8 model is a simple validated and accurate tool for preoperative stratification of early recurrence of HCC after LT.

scholarly journals Preoperative Risk Stratification for Early Recurrence of HBV-related Hepatocellular Carcinoma After Deceased Donor Liver Transplantation:A Five-Eight Model Development and Validation

2019 ◽  
Author(s):  
Abdulahad Abdulrab Mohammed Al-Ameri ◽  
Xuyong Wei ◽  
Lidan Lin ◽  
Zhou Shao ◽  
Haijun Guo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Model Development ◽  
Deceased Donor ◽  
Early Recurrence ◽  
Individual Risk ◽  
Tumor Diameter ◽  
Tumor Number ◽  
Serum Alpha Fetoprotein ◽  
Transplant Registry ◽  
Development And Validation

Abstract Background Early recurrence of hepatocellular carcinoma (HCC) after liver transplantation (LT) is associated with poor surgical outcomes. Objectives To construct a preoperative model to predict individual risk of post-LT HCC recurrence. Methods Data of 748 adult patients who underwent deceased donor LT for HCC between January 2015, and February 2019 were collected retrospectively from the China Liver Transplant Registry database and randomly divided into training (n=486) and validation(n=262) cohorts. A multivariate analysis was performed and the five-eight model was developed. Results A total of 748 patients were included in the study; of them, 96% had hepatitis B virus (HBV) and 84 % had cirrhosis. Pre-LT serum alpha-fetoprotein (AFP), tumor number and largest tumor diameter were incorporated to construct the 5-8 model which can stratify patients accurately according to their risk of recurrence into three prognostic subgroups; low-(0-5 points), medium-(6–8 points) and high-risk (>8 points) with 2-year post-LT recurrence rate of (5%,20% and 51%,p<0.001) respectively.The 5-8 model was better than Milan, Hangzhou, and AFP-model for prediction of HCC early recurrence. These findings were confirmed by the results of the validation cohort. Conclusions The 5-8 model is a simple validated and accurate tool for preoperative stratification of early recurrence of HCC after LT.

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