Anoxybacillus sp. AH1, an α-amylase-producing thermophilic bacterium isolated from Dargeçit hot spring

Biologia ◽  
2015 ◽  
Vol 70 (7) ◽  
Author(s):  
Ömer Acer ◽  
Hemşe Pırınççiğlu ◽  
Fatma Matpan Bekler ◽  
Reyhan Gül-Güven
Keyword(s):  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Potato Starch ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Thermophilic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Optimum Growth Temperature

AbstractThe present study was conducted to isolate α-amylase-producing thermophilic bacteria from Darge¸cit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60ºC and 7.0-7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular α-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased α-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl

scholarly journals Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

2019 ◽  
Vol 23 (1) ◽  
pp. 11-18
Author(s):  
Fangfang Cheng ◽  
He Chen ◽  
Ni Lei ◽  
Meng Zhang ◽  
Hongchang Wan
Keyword(s):  
Lactobacillus Plantarum ◽  
Specific Activity ◽  
Cell Envelope ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Carbon And Nitrogen Sources ◽  
Individual Factor ◽  
Carbon And Nitrogen ◽  
Mrs Broth

Abstract In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey protein, soy peptone, yeast, tryptone, beef extract and peptone) on activity of cell envelope proteinases (CEP), specific activity, protein content, OD600 value and pH in MRS broth fermented by Lactobacillus plantarum LP69 were investigated by individual factor experiment. The results indicated that carbon and nitrogen sources have significant influence on the activity of CEP and specific activitys of L. plantarum LP69, glucose, maltose, casein peptone and peptone are superior to other selected carbon and nitrogen sources. The optimum concentrations of glucose, maltose, casein peptone and peptone for L. plantarum LP69 are 2%, 2%, 1% and 1%; the activity of CEP are 19.52U/mL, 21.13U/mL, 13.49U/mL and 20.61U/mL, respectively.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

scholarly journals Optimising carbon and nitrogen sources for Azotobacter chroococcum growth

2011 ◽  
Vol 10 (15) ◽  
pp. 2951-2958 ◽  
Author(s):  
Gutieacute rrez Rojas Ivonne ◽  
Beatriz Torres Geraldo Ana ◽  
Moreno Sarmiento Nubia
Keyword(s):  
Nitrogen Sources ◽  
Azotobacter Chroococcum ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

Metabolic Flux Analysis and Optimal Control in the Process of Ectoine Fermentation

2011 ◽  
Vol 393-395 ◽  
pp. 851-854
Author(s):  
Lin Hua Zhang ◽  
Xin Zheng ◽  
Ya Jun Lang
Keyword(s):  
Optimal Control ◽  
Batch Fermentation ◽  
Metabolic Flux ◽  
Monosodium Glutamate ◽  
Nitrogen Sources ◽  
Flux Analysis ◽  
Key Factors ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Key Nodes

In this study, the metabolic network of ectoine by Halomonas venusta DSM 4743 was established. The key nodes to influence the ectoine fermentation in metabolic flux and the basis during optimal control of fermentation process were investigated. The results showed that G6P, α-KG and OAA nodes were the key factors to influence the synthesis of ectoine. The metabolic flux distributions at the key nodes were significantly improved and ectoine concentration was enhanced in ectoine fermentation by adopting monosodium glutamate as the sole carbon and nitrogen sources, feeding monosodium glutamate and supplying oxygen limitedly. The batch fermentation was carried out in 10 L fermentor , the concentration and yield of ectoine was 8.4 g/L and 0.1 g/g, respectively, which were increased by 2.8 and 2 times, by comparison with batch fermentation using glucose as carbon source.

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