scholarly journals Pneumatic tube transportation of urine samples

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Eline Sandvig Andersen ◽  
Ivan Brandslund
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Urine Samples ◽  
Tube System ◽  
Pneumatic Tube ◽  
Local Quality ◽  
Renal Tubular ◽  
Pneumatic Tube System

AbstractObjectivesPneumatic tube transportation of samples is an effective way of reducing turn-around-time, but evidence of the effect of pneumatic tube transportation on urine samples is lacking. We thus wished to investigate the effect of pneumatic tube transportation on various components in urine, in order to determine if pneumatic tube transportation of these samples is feasible.MethodsOne-hundred fresh urine samples were collected in outpatient clinics and partitioned with one partition being carried by courier to the laboratory, while the other was sent by pneumatic tube system (Tempus600). Both partitions were then analysed for soluble components and particles, and the resulting mean difference and limits of agreement were calculated.ResultsAlbumin, urea nitrogen, creatinine, protein and squamous epithelial cells were unaffected by transportation in the Tempus600 system, while bacteria, renal tubular epithelial cells, white blood cells and red blood cells were affected and potassium and sodium may have been affected.ConclusionsThough pneumatic tube transportation did affect some of the investigated components, in most cases the changes induced were clinically acceptable, and hence samples could be safely transported by the Tempus600 pneumatic tube system. For bacteria, white blood cells and red blood cells local quality demands will determine if pneumatic tube transportation is appropriate.

Improved Identification of White Blood Cells and Renal Tubular Epithelial Cells in Urine Using Cytology

2011 ◽  
Vol 140 ◽  
pp. 152-156
Author(s):  
Xu Guo Sun ◽  
Dian Jun Wei ◽  
Xiao Chun Liu ◽  
Yu Zhu Ma ◽  
Xin Fan ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Automatic Identification ◽  
Identification System ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Urine Sediment ◽  
Cytoplasmic Staining ◽  
Renal Tubular

Objectives: Conventional automatic identification system to differentiate white blood cells from renal tubular epithelial cells was limited by overlapping parameters and investigation of clear classification of these two cells could be critical to diagnosis and prognosis. Methods: Urine samples from 120 individuals (30 bladder cystitis, 30 glomerular nephritis, 30 pyelonephritis and 30 nephrotic syndrome) were collected. Urine sediments were stained by Sternheimer method and examined by TJYDSXG-1 microscopic cell analysis system including cell size, degree of cytoplasmic staining and nuclear coefficient of variation (CV). Peroxidase chemical staining was also employed to differentiate white blood cells (WBC) and renal tubular epithelial cells (RTEC) in sediments. Results: WBC in urine sediment was (8-13) μm, while (10-16) μm for RTEC, with 36% overlapping of nuclear CV. Peroxidase chemical staining intensity index is 0-4 for WBC and 0-1 for RTEC. Conclusions: Percentage of overlap between WBC and RTEC can be reduced to 7%-13% when Sternheimer staining was combined with peroxidase staining.

scholarly journals Enhancing the Detection of Dysmorphic Red Blood Cells and Renal Tubular Epithelial Cells with a Modified Urinalysis Protocol

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yu Chu-Su ◽  
Kenichi Shukuya ◽  
Takashi Yokoyama ◽  
Wei-Chou Lin ◽  
Chih-Kang Chiang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular

The Effect of Red Blood Cells on the ADP-induced Aggregation of Human Platelets in Flow Through Tubes

1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith
Keyword(s):  
Platelet Aggregation ◽  
Shear Rate ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Mean Transit Time ◽  
White Blood Cells ◽  
Aggregate Size ◽  
Human Platelets ◽  
Induced Aggregation

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

Impact of pneumatic tube system transport for the monitoring of heparin therapy

2017 ◽  
Vol 158 ◽  
pp. 35-37
Author(s):  
Aurélien Lebreton ◽  
Alessandro Casini ◽  
Oana Bulla ◽  
Pierre Fontana
Keyword(s):  
Heparin Therapy ◽  
Tube System ◽  
Pneumatic Tube ◽  
Pneumatic Tube System
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