Reference intervals for thyreotropin and thyroid hormones for healthy adults based on the NOBIDA material and determined using a Modular E170®

2008 ◽  
Vol 46 (9) ◽  
Author(s):  
Lennart Friis-Hansen ◽  
Linda Hilsted
Keyword(s):  
Thyroid Hormones ◽  
Reference Interval ◽  
Reference Intervals ◽  
Healthy Adults ◽  
Free Triiodothyronine ◽  
Thyroglobulin Antibodies ◽  
Men And Women ◽  
Blood Samples ◽  
Upper Limit

Abstract: The aim of the present study was to establish Nordic reference intervals for thyreotropin (TSH) and the thyroid hormones in heparinized plasma.: We used 489 heparinized blood samples, collected in the morning, from the Nordic NOBIDA reference material, from healthy adults without medication. TSH, thyroxine, free thyroxine, triiodothyronine, free triiodothyronine, and thyroglobulin antibodies (Tg-ab) were measured using assays for Roche Modular E170: The measured concentrations for the thyroid hormones, but not TSH, followed a Gaussian distribution. There were more TPO-ab and Tg-ab positive women than men. After exclusion of the TPO-ab and the Tg-ab positive individuals, the reference interval TSH was 0.64 (0.61–0.72) to 4.7 (4.4–5.0) mIU/L. The exclusion of these ab-positive samples also minimized the differences in TSH concentrations between the sexes and the different Nordic countries. For the thyroid hormones, there were only minor differences between the reference intervals between the Nordic populations and between men and women. These reference intervals were unaffected by removal of the TPO-ab and TG-ab positive samples.: The upper limit of the TSH reference interval in our study is high compared to some other recent reports. This could be due to blood sampling in the morning. Furthermore, the Roche platform gives slightly higher results than other platforms. The number and distribution of the samples in the NOBIDA material makes it suitable for the determination of hormone Nordic reference intervals.Clin Chem Lab Med 2008;46:1305–12.

scholarly journals First- and Second-Trimester Reference Intervals for Thyroid Hormones during Pregnancy in “Rhea” Mother-Child Cohort, Crete, Greece

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Polyxeni Karakosta ◽  
Leda Chatzi ◽  
Emmanouil Bagkeris ◽  
Vasiliki Daraki ◽  
Dimitris Alegakis ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Reference Interval ◽  
Reference Intervals ◽  
Thyroid Stimulating Hormone ◽  
Specific Reference ◽  
Reference Ranges ◽  
Thyroid Function Tests ◽  
Second Trimester ◽  
Free Triiodothyronine ◽  
Reference Limit

Estimation and interpretation of thyroid function tests in pregnant women is of utmost importance for maternal, fetal and neonatal health. Our objective was to calculate laboratory- and geography-specific reference intervals for thyroid hormones during pregnancy in an iodine-sufficient area of the Mediterranean, Crete, Greece. This project was performed in the context of “Rhea” mother-child cohort. Fulfillment of extensive questionnaires and estimation of free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), and antithyroid antibodies were performed. The reference population was defined using inclusion criteria regarding thyroidal, obstetric, and general medical status of women. Reference interval for TSH was 0.05–2.53 μIU/mL for the first and 0.18–2.73 μIU/mL for the second trimester. 6,8% and 5,9% of women in the first and second trimester, respectively, had TSH higher than the upper reference limit. These trimester-specific population-based reference ranges are essential in everyday clinical practice for the correct interpretation of thyroid hormone values and accurate classification of thyroid disorders.

scholarly journals Reference Intervals of Thyroid Hormones and Correlation of BMI with Thyroid Function in Healthy Zhuang Ethnic Pregnant Women

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Yonghong Sheng ◽  
Dongping Huang ◽  
Shun Liu ◽  
Xuefeng Guo ◽  
Jiehua Chen ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Pregnant Women ◽  
Thyroid Function ◽  
Reference Intervals ◽  
Thyroid Stimulating Hormone ◽  
Specific Reference ◽  
American Thyroid Association ◽  
Reference Ranges ◽  
Free Triiodothyronine ◽  
Cross Sectional

Ethnic differences in the level of thyroid hormones exist among individuals. The American Thyroid Association (ATA) recommends that an institution or region should establish a specific thyroid hormone reference value for each stage of pregnancy. To date, a limited number of studies have reported the level of thyroid hormones in Chinese minorities, and the exact relationship between BMI and thyroid function in pregnant women is ill. This study was performed to establish trimester-specific reference ranges of thyroid hormones in Zhuang ethnic pregnant women and explore the role of body mass index (BMI) on thyroid function. A total of 3324 Zhuang ethnic health pregnant women were recruited in this Zhuang population-based retrospective cross-sectional study. The values of thyroid stimulating hormone (TSH), free thyroxine (FT4), and free triiodothyronine (FT3) were determined by automatic chemiluminescence immunoassay analyzer. Multivariate linear regression and binary logistic regression were constructed to evaluate the influence of BMI on the thyroid function. The established reference intervals for the serum thyroid hormones in three trimesters were as follows: TSH, 0.02–3.28, 0.03–3.22, and 0.08-3.71 mIU/L; FT4, 10.57–19.76, 10.05–19.23, and 8.96–17.75 pmol/L; FT3, 3.51–5.64, 3.42–5.42, and 2.93–5.03 pmol/L. These values were markedly lower than those provided by the manufacturers for nonpregnant adults which can potentially result in 6.10% to 19.73% misclassification in Zhuang pregnant women. Moreover, BMI was positively correlated with isolated hypothyroxinemia (OR=1.081, 95% CI=1.007–1.161), while the correlation between the BMI and subclinical hypothyroidism was not statistically significant (OR=0.991, 95% CI=0.917–1.072). This is the first study focusing on the reference ranges of thyroid hormones in Guangxi Zhuang ethnic pregnant women, which will improve the care of them in the diagnosis and treatment. We also found that high BMI was positively associated with the risk of isolated hypothyroxinemia.

Optimized assay for serum angiotensin-converting enzyme activity.

1981 ◽  
Vol 27 (12) ◽  
pp. 2048-2052 ◽  
Author(s):  
P L Hurst ◽  
C J Lovell-Smith
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Chloride Ion ◽  
Reference Intervals ◽  
Colorimetric Determination ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme Activity ◽  
Kinetic Assay ◽  
Serum Angiotensin Converting Enzyme ◽  
Men And Women

Abstract This optimized two-point kinetic assay for serum angiotensin-converting enzyme is based on the colorimetric determination of hippurate with cyanuric chloride/dioxan reagent. The hippurate is released from hippuryl-L-histidyl-L-leucine by angiotensin-converting enzyme in the presence of chloride ion. CVs for the method (within-run and between-run) ranged from 2.1 to 3.2%. Linearity extends up to 200 U/L. Results are unaffected by lipemia and icterus. Hemoglobin in concentrations greater than 1.5 g/L causes a slight negative interference. Reference intervals for men and women are 22-82 U/L and 25-69 U/L, respectively.

scholarly journals Demonstration of reciprocal diurnal variation in human serum T3 and rT3 concentration demonstrated by mass spectrometric analysis and establishment of thyroid hormone reference intervals

2020 ◽  
Vol 11 ◽  
pp. 204201882092268
Author(s):  
Qian Sun ◽  
Lívia Avallone ◽  
Brian Stolze ◽  
Katherine A. Araque ◽  
Yesim Özarda ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Hormones ◽  
Reference Intervals ◽  
Diurnal Fluctuation ◽  
Spectrometric Analysis ◽  
Serum Samples ◽  
Free Triiodothyronine ◽  
Reverse T3 ◽  
Wide Range ◽  
Total Thyroxine

Background: There has been a wide range of reference intervals proposed in previous literature for thyroid hormones due to large between-assay variability of immunoassays, as well as lack of correction for collection time. We provided the diurnal reference intervals for five thyroid hormones, namely total thyroxine (TT4), total triiodothyronine (TT3), free thyroxine (FT4), free triiodothyronine (FT3), and reverse T3 (rT3), measured in serum samples of healthy participants using a liquid chromatography/tandem mass spectrometry (LC-MS/MS) method. Methods: Couplet serum samples (a.m. and p.m.) were collected from 110 healthy females and 49 healthy males. Healthy volunteers were recruited from four participating centers between 2016 and 2018. Measurements of thyroid hormones were obtained by LC-MS/MS analysis. Results: Our study revealed significant uptrend in AM to PM FT4 ( p < 0.0001) samples, downtrend in AM to PM TT3 ( p = 0.0004) and FT3 samples ( p < 0.0001), and AM to PM uptrend in rT3 samples ( p < 0.0001). No difference was observed for TT4 between AM and PM. No significant sex differences were seen for any of the five thyroid hormones. Conclusion: When diagnosing thyroid disorders, it is important to have accurate measurement of thyroid hormones, and to acknowledge the diurnal fluctuation found, especially for FT3. Our study highlights the importance of standardization of collection times and implementation of LC-MS/MS in thyroid hormone measurement.

Rapid determination of serum myoglobin with a routine chemistry analyzer

1993 ◽  
Vol 39 (4) ◽  
pp. 653-658 ◽  
Author(s):  
A J Bakker ◽  
D A Boymans ◽  
D Dijkstra ◽  
J P Gorgels ◽  
R Lerk
Keyword(s):  
Predictive Value ◽  
Rapid Determination ◽  
Reference Interval ◽  
High Dose ◽  
Reference Limit ◽  
Hook Effect ◽  
Upper Limit ◽  
Upper Reference Limit ◽  
Serum Myoglobin

Abstract A turbidimetric immunoassay system (Turbitime system, Behringwerke AG) allows rapid determination of myoglobin in serum. We adapted the reagents for this myoglobin assay (Turbiquant myoglobin) for use with a Hitachi 717 analyzer. No high-dose hook effect was observed up to 15,000 micrograms/L. Interassay CVs were 4.6% (mean = 72.0 micrograms/L; n = 9) and 2.5% (mean = 365.6 micrograms/L; n = 11). The calibration curve was stable for at least 1 month. Hemolysis did not interfere, and turbidity from lipemia interfered only when absorbance exceeded 2.0 A. Results of this method (y) correlated well with those by the Turbitime method (y = 1.256x - 44.1 micrograms/L; n = 91; r = 0.991) and by a commercially available radioimmunoassay (Byk-Sangtec; y = 0.739x - 42.2 micrograms/L; n = 94; r = 0.991). The upper limit (95th percentile) of the reference interval for myoglobin was estimated at 57.9 micrograms/L. The positive predictive value for results of myoglobin at admission was 89% with this upper reference limit and 99% with 100 micrograms/L, whereas the negative predictive value was about 60% for both limits.

Methods compared for determining glutathione peroxidase activity in blood.

1987 ◽  
Vol 33 (4) ◽  
pp. 539-543 ◽  
Author(s):  
B Faraji ◽  
H K Kang ◽  
J L Valentine
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Reference Intervals ◽  
The Other ◽  
Specific Time ◽  
Glutathione Peroxidase Activity ◽  
Time Intervals ◽  
Indirect Methods ◽  
Blood Samples

Abstract We compared four methods for determination of glutathione peroxidase (EC 1.11.1.9) activity, using blood samples from 52 healthy volunteers. Two methods depended on direct assay of the amount of glutathione remaining at specific time intervals; the two indirect methods involved measuring the rate of disappearance of NADPH. We assessed the precision and reproducibility of each method. One of the indirect assays proved to be far superior to the other methods. Results of each of the methods were correlated with one another. We present the normal reference intervals for glutathione peroxidase activity for all four methods.

scholarly journals Determination of adjusted reference intervals of urinary biomarkers of oxidative stress in healthy adults using GAMLSS models

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206176 ◽  
Author(s):  
Liliya Chamitava ◽  
Vanessa Garcia-Larsen ◽  
Lucia Cazzoletti ◽  
Paolo Degan ◽  
Andrea Pasini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reference Intervals ◽  
Healthy Adults ◽  
Urinary Biomarkers ◽  
Biomarkers Of Oxidative Stress

Performance and Clinical Utility of a Commercially Available ‘C-terminal’ PTH Assay

1986 ◽  
Vol 23 (4) ◽  
pp. 434-439 ◽  
Author(s):  
A R Ingle ◽  
J E Bailey ◽  
H L Matthews ◽  
J S Harrop
Keyword(s):  
Renal Failure ◽  
Parathyroid Hormone ◽  
Clinical Utility ◽  
Blood Samples ◽  
Upper Limit ◽  
Whole Blood Samples ◽  
Serum Pth ◽  
Pth Radioimmunoassay ◽  
Detectable Serum

The performance and clinical utility of a ‘C-terminal’ parathyroid hormone (PTH) radioimmunoassay (Dac-Cel, Wellcome Diagnostics) is described. Parathyroid hormone, as measured by the Dac-Cel method, is stable in whole blood samples for at least 24 h. 84% of patients with hypercalcaemia due to primary hyperparathyroidism have values above the upper limit seen in normocalcaemic subjects (0·5 μg/L), with detectable serum PTH demonstrable in the remaining 16%. In patients with hypocalcaemia due to hypoparathyroidism serum PTH was undetectable in 73% and ‘inappropriately’ low in the remainder. In 50% of patients with malignancy-associated hypercalcaemia serum PTH was undetectable, but was above 0·5 μg/L in 13%. Increased PTH concentrations were invariably found in patients with renal failure. The Dac-Cel method is a reliable and robust technique for measurement of PTH and in conjunction with determination of calcium facilitates the diagnosis of primary parathyroid disorders. Caution is required in the interpretation of PTH measurements in patients with renal failure; the significance of detectable PTH in some patients with malignancy-associated hypercalcaemia is not clear.

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