scholarly journals A survey of bacterial, fungal and plant metabolites against Aedes aegypti (Diptera: Culicidae), the vector of yellow and dengue fevers and Zika virus

2017 ◽  
Vol 15 (1) ◽  
pp. 156-166 ◽  
Author(s):  
Marco Masi ◽  
Alessio Cimmino ◽  
Nurhayat Tabanca ◽  
James J. Becnel ◽  
Jeffrey R. Bloomquist ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Methyl Esters ◽  
Fusaric Acid ◽  
Low Molecular Weight ◽  
Plant Metabolites ◽  
Amaryllidaceae Alkaloids ◽  
Adulticidal Activity ◽  
Nitropropionic Acid ◽  
Mosquitocidal Activity ◽  
Cytochalasin A

AbstractAedes aegypti L. is the major vector of the arboviruses responsible for dengue fever, one of the most devastating human diseases. Some bacterial, fungal and plant metabolites belonging to different chemical subgroups, including Amaryllidaceae alkaloids, anthracenes, azoxymethoxytetrahydropyrans, cytochalasans, 2,5-diketopiperazines, isochromanones, naphthoquinones, organic small acids and their methyl esters, sterols and terpenes including sesquiterpenes and diterpenes, were tested for their larvicidal and adulticidal activity against Ae. aegypti. Out of 23 compounds tested, gliotoxin exhibited mosquitocidal activity in both bioassays with an LC50 value of 0.0257 ± 0.001 µg/µL against 1st instar Ae. aegypti and LD50 value of 2.79 ± 0.1197 µg/mosquito against adult female Ae. aegypti. 2-Methoxy-1,4-naphthoquinone and cytochalasin A showed LC50 values of 0.0851 ± 0.0012 µg/µL and 0.0854 ± 0.0019 µg/µL, respectively, against Ae. aegypti larvae. In adult bioassays, fusaric acid (LD50= 0.8349 ± 0.0118 µg/mosquito), 3-nitropropionic acid (LD50 = 1.6641 ± 0.0494 µg/mosquito) and α-costic acid (LD50 = 2.547 ± 0.0835 µg/mosquito) exhibited adulticidal activity. Results from the current study confirm that compounds belonging to cytochalsin, diketopiperazine, naphthoquinone and low molecular weight organic acid groups are active and may stimulate further SAR investigations.

scholarly journals Larvicidal Property of the Extract and Fractions of Hannoa klaineana against the Larvae of Aedes aegypti

2020 ◽  
pp. 127-132
Author(s):  
Ifeoma Chioma Ibe ◽  
Eze Elijah Ajaegbu ◽  
Lame Younoussa ◽  
Simon Pierre Yinyang Danga ◽  
Christopher Obidike Ezugwu
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Control Programme ◽  
Chloroform Fraction ◽  
Plant Metabolites ◽  
Lc50 Value ◽  
Synthetic Materials ◽  
Control Programmes ◽  
Crude Methanol Extract

Aedes aegypti (Culicidae) is the main vector for dengue which is endemic in Nigeria and Africa at large. Use of synthetic materials for the control of this vector has always proven abortive. The introduction of plant metabolites which is biodegradable maybe successful and safer to the human health in the control programme of this vector since plants are very rich in bioactive secondary metabolites. Hence, we evaluated the larvicidal activity of the crude methanol extract, n-hexane, chloroform, ethyl acetate and methanol fractions of Hannoa klaineana (Simaroubaceae) against the IV instar larvae of A. aegypti mosquitousing standard protocols. The most active fractions were  n-hexane, chloroform and ethyl acetate fractions with LC50 values of 1.006, 1.479, and 3.198 mg/ml respectively; n-hexane fraction having the highest property with LC50 value of 1.006 mg/ml and followed by chloroform fraction with LC50 value of 1.479 mg/ml. The evaluation has shown that the secondary metabolites of H. klaineana can be used as an alternative for the synthetic larvicides which its use has proven abortive in the vector control programmes.

scholarly journals LARVICIDAL ACTIVITY OF Caesalpinia ferrea MART. AND Lippia origanoides CHAM. AGAINST Aedes aegypti (LINNAEUS, 1762) (DIPTERA: CULICIDAE)

2020 ◽  
Vol 49 (1) ◽  
pp. 45-54
Author(s):  
Thiago Andre Santos de Andrade ◽  
Ivanise Maria de Santana ◽  
George Chaves Jimenez ◽  
Eulina Tereza Nery Farias ◽  
Lucia Oliveira de Macedo ◽  
...  
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Botanical Insecticide ◽  
Plant Metabolites ◽  
Important Public Health ◽  
Public Health Challenges ◽  
Ethanol Extracts

The control of Aedes aegypti has been considered one of the most important public health challenges worldwide. Chemical compounds have long been used for this purpose, but resistance to these molecules has also increased. Therefore, over the last few years several studies have focused on the development of alternative tools, particularly those based on plant metabolites. The purpose of this study was to assess the larvicidal activity of Caesalpinia ferrea and Lippia origanoides against Ae. aegypti. Larvae (L3) of Ae. aegypti Liverpool and Rockefeller strains, as well as of the Recife population were exposed to different concentrations  of C. ferrea (ranging from 13.1 to 105 mg/mL) and L. origanoides (ranging from 16.3 to 130 mg/mL), and the mortality rate was evaluated up to 48 hours after the beginning of the experiment. All tested groups and  ontrol group were quadruplicated. For C. ferrea, mortality ranged from 42.5% to 100% for Ae. aegypti Liverpool strain,from 67% to 100% for Ae. aegypti Rockfeller strain, and 57% to 100% for Ae. aegypti Recife population after 48 hours of larval exposure. For L. origanoides, the larvicidal activity ranged from 75% to 100% for Ae. aegypti Liverpool strain, from 61.5% to 100% for Ae. aegypti Rockfeller strain, and from 60.5% to 100% for Ae.aegypti Recife population. The hydro ethanol extract of C. ferrea and L. origanoides presented larvicidal activity against Ae. aegypti.KEY WORDS: Aedes aegypti; hydro ethanol extracts; Caesalpinia ferrea; Lippia sidoides; botanical insecticide; mosquitoes.

Larvicidal, pupicidal, repellent and adulticidal activity of Citrus sinensis orange peel extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

2012 ◽  
Vol 111 (4) ◽  
pp. 1757-1769 ◽  
Author(s):  
Kadarkarai Murugan ◽  
Palanisamy Mahesh Kumar ◽  
Kalimuthu Kovendan ◽  
Duraisamy Amerasan ◽  
Jayapal Subrmaniam ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Citrus Sinensis ◽  
Anopheles Stephensi ◽  
Orange Peel ◽  
Adulticidal Activity ◽  
Orange Peel Extract ◽  
Peel Extract

scholarly journals Xanthine dehydrogenase‐1 silencing in Aedes aegypti mosquitoes promotes a blood feeding–induced adulticidal activity

2017 ◽  
Vol 31 (6) ◽  
pp. 2276-2286 ◽  
Author(s):  
Jun Isoe ◽  
Natthida Petchampai ◽  
Yurika E. Isoe ◽  
Katrina Co ◽  
Stacy Mazzalupo ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Blood Feeding ◽  
Xanthine Dehydrogenase ◽  
Adulticidal Activity

scholarly journals Enhancement of Cry19Aa Mosquitocidal Activity against Aedes aegypti by Mutations in the Putative Loop Regions of Domain II

2004 ◽  
Vol 70 (6) ◽  
pp. 3769-3771 ◽  
Author(s):  
Mohd Amir F. Abdullah ◽  
Donald H. Dean
Keyword(s):  
Bacillus Thuringiensis ◽  
Protein Engineering ◽  
Aedes Aegypti ◽  
Rational Design ◽  
Surface Loop ◽  
Mosquitocidal Activity ◽  
Loop Regions

ABSTRACT Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or Culex.

scholarly journals Potential of Cry10Aa and Cyt2Ba, Two Minority δ-endotoxins Produced by Bacillus thuringiensis ser. israelensis, for the Control of Aedes aegypti Larvae

Toxins ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 355 ◽  
Author(s):  
Daniel Valtierra-de-Luis ◽  
Maite Villanueva ◽  
Liliana Lai ◽  
Trevor Williams ◽  
Primitivo Caballero
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Recombinant Proteins ◽  
Synergistic Activity ◽  
Minor Components ◽  
Parasporal Crystal ◽  
Synergistic Interactions ◽  
Bt Toxins ◽  
Mosquitocidal Activity ◽  
The Individual

Bacillus thuringiensis ser. israelensis (Bti) has been widely used as microbial larvicide for the control of many species of mosquitoes and blackflies. The larvicidal activity of Bti resides in Cry and Cyt δ-endotoxins present in the parasporal crystal of this pathogen. The insecticidal activity of the crystal is higher than the activities of the individual toxins, which is likely due to synergistic interactions among the crystal component proteins, particularly those involving Cyt1Aa. In the present study, Cry10Aa and Cyt2Ba were cloned from the commercial larvicide VectoBac-12AS® and expressed in the acrystalliferous Bt strain BMB171 under the cyt1Aa strong promoter of the pSTAB vector. The LC50 values for Aedes aegypti second instar larvae estimated at 24 hpi for these two recombinant proteins (Cry10Aa and Cyt2Ba) were 299.62 and 279.37 ng/mL, respectively. Remarkable synergistic mosquitocidal activity was observed between Cry10Aa and Cyt2Ba (synergistic potentiation of 68.6-fold) when spore + crystal preparations, comprising a mixture of both recombinant strains in equal relative concentrations, were ingested by A. aegypti larvae. This synergistic activity is among the most powerful described so far with Bt toxins and is comparable to that reported for Cyt1A when interacting with Cry4Aa, Cry4Ba or Cry11Aa. Synergistic mosquitocidal activity was also observed between the recombinant proteins Cyt2Ba and Cry4Aa, but in this case, the synergistic potentiation was 4.6-fold. In conclusion, although Cry10Aa and Cyt2Ba are rarely detectable or appear as minor components in the crystals of Bti strains, they represent toxicity factors with a high potential for the control of mosquito populations.

HPLC analysis of fusaric acid, 9,10-dehydrofusaric acid and their methyl esters, toxic metabolites from weed pathogenic Fusarium species

2002 ◽  
Vol 13 (5) ◽  
pp. 277-282 ◽  
Author(s):  
Carmine Amalfitano ◽  
Raffaele Pengue ◽  
Anna Andolfi ◽  
Maurizio Vurro ◽  
Maria C. Zonno ◽  
...  
Keyword(s):  
Hplc Analysis ◽  
Fusarium Species ◽  
Methyl Esters ◽  
Fusaric Acid ◽  
Toxic Metabolites

Larvicidal Activity of Secondary Plant Metabolites in Aedes aegypti Control: An Overview of the Previous 6 Years

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986289
Author(s):  
Aline Camargo Jesus de Souza Wuillda ◽  
Roberto Carlos Campos Martins ◽  
Fernanda das Neves Costa
Keyword(s):  
Aedes Aegypti ◽  
Mechanisms Of Action ◽  
World Population ◽  
Plant Metabolites ◽  
Resistance Development ◽  
Natural Sources ◽  
Secondary Plant Metabolites ◽  
Structure Activity ◽  
The World ◽  
Continuous Application

Aedes aegypti is the main transmitter of several arboviruses, mainly dengue. It occurs, recently, in more than 100 countries and majority of the world population lives in areas of mosquito incidence, marking its control relevant and necessary. Presently, the main form of vector control is the use of synthetic insecticides; however, its continuous application has led to inefficiency due to resistance development. Based on this fact, the insecticides from natural sources appear as a friendly alternative for man and the environment. This study provides an overview of the larvicidal compounds isolated from plant extracts while controlling A. aegypti, in the previous 6 years (2013-2018), and aims to impart more knowledge regarding the described metabolites and to encourage the search for new bioactive compounds. In addition, the proposals for mechanisms of action and structure-activity relationships that may justify the larvicidal potential are also discussed.

scholarly journals Adulticidal Activity of Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Native Strains From Argentina Against Aedes aegypti (Diptera: Culicidae)

2019 ◽  
Vol 57 (2) ◽  
pp. 636-640
Author(s):  
Marianel L Falvo ◽  
Anahi Musso ◽  
Eliana Ordoqui ◽  
Claudia C López Lastra ◽  
Christian Luz ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Metarhizium Anisopliae ◽  
Virulent Strain ◽  
Survival Functions ◽  
Survival Times ◽  
Promising Candidate ◽  
Adulticidal Activity ◽  
Kaplan Meier ◽  
The Mean ◽  
Native Strains

Abstract The adulticidal activity of six fungal strains of Metarhizium anisopliae (Metschn.) Sorokin s.l. (Hypocreales: Clavicipitaceae) against Aedes aegypti (L.) (Diptera: Culicidae) was assessed. These strains (CEP 085, CEP 087, CEP 120, CEP 350, CEP 404, and CEP 423) were isolated from soil samples or nondipteran hosts collected from areas in Argentina where Ae. aegypti is distributed. Bioassays were performed with four conidial concentrations plus a control of each fungal strain to determine the lethal concentrations (LC50/LC90), the median survival times (ST50), the mean percentage of the surviving individuals, and the mean percentage of mycotized cadavers. The strains were able to infect and kill adult Ae. aegypti. Significant differences were found among the LC50 values, with CEP 423 as the most virulent strain with the lowest LC50 (2.4 × 106 conidia/ml). At 1 × 107 conidia/ml: no significant differences were found in the Kaplan–Meier survival functions among the strains; the ST50 ranged from 5 (CEP 085) to 8 d (CEP 350); and the mean percentage of the surviving adults was between 13.3 (CEP 085, CEP 350 and CEP 423) and 46.7% (CEP 087). Significant differences were also found among strains in the mean percentage of cadavers with fungal sporulation. Strain CEP 423 produced the highest percentage of mycotized adults (70%). The concentration of the inoculum significantly affected the survival of individuals and the percentage of mycotized cadavers within each strain. Metarhizium anisopliae s.l. CEP 423 was selected as the most promising candidate for further research aiming to develop a mycoinsecticide against Ae. aegypti.

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