Morphological and Molecular Characterization of Heterobothrium indicus n. sp. (Monogenea: Diclidophoridae) and Its Phylogenetic Status

2021 ◽  
Author(s):  
A.K. Verma
Keyword(s):  
New Species ◽  
18S Rdna ◽  
Arabian Sea ◽  
28S Rdna ◽  
Coi Gene ◽  
Western Coast ◽  
Monogenean Parasite ◽  
Morphological Studies ◽  
Phylogenetic Status

Background: A new species Heterobothrium indicus n. sp. was isolated from the gills of Upeneus moluccensis (Bleeker, 1855) from the Western Coast of India in Arabian Sea region. The monogenean parasite differs from other congeners by morphological features like the presence of asymmetrical haptoral region, 4th pair of clamp smallest than other three pairs of clamps, genital atrium with 8 hooks, number of testes 29-37 and absence of isthmus.Methods: During the survey of marine fishes at Arabian Sea region, new species of monogenean parasite was isolated from the gills of marine fish Upeneus moluccensis. The parasites were morphologically characterized with the help of light and phase contrast microscopy. 18S rDNA, 28S rDNA, mt COI, ITS1+5.8S and ITS2+5.8S gene regions of parasites were amplified, sequenced and compared with other diclidophorid taxa using different bioinformatics tools.Result: Phylogenetic tree analyses (NJ, ME and MP methods) of 18S rDNA, 28S rDNA and mt COI gene regions are complementing the morphological studies and clearly suggested the placement of this new species under subfamily Choricotylinae, family Diclidophoridae.

Morphological and molecular characterization of Paractinolaimus sahandi n. sp. (Nematoda: Actinolaimidae) from the Sahand Mountains in Iran

2010 ◽  
Vol 85 (3) ◽  
pp. 276-282 ◽  
Author(s):  
M. Pedram ◽  
G. Niknam ◽  
M.T. Vinciguerra ◽  
W. Ye ◽  
R.T. Robbins
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Outer Layer ◽  
18S Rdna ◽  
Soil Samples ◽  
28S Rdna ◽  
First Report ◽  
Morphological And Molecular Characterization

AbstractParactinolaimus sahandi n. sp., found in wet soil samples collected from the rhizosphere of grasses of Sahand Mountains, Iran, is described. This new species is characterized by its long body (3.5–4.7 mm), high a value (74.5–88.5), anterior location of posterior subventral nuclei, occupying 62.5–68.0% of glandularium distance, the presence of 1–4 pre- and 1–3 post-vulval papillae and numerous tiny, not innervated papillae in front and behind the vulva in the outer layer of cuticle; common functional males in the population, with 62.5–81.3 μm long spicules and 15–17 ventromedian supplements. The new species, which is the only one in the genus showing the advulval cuticular tiny papillae and is unusually slender, is compared to four species of Paractinolaimus, namely P. macrolaimus, P. longidrilus, P. spanithelus and P. rafiqi. The ribosomal 18S rDNA (1246 bp sequenced) and 28S rDNA D2/D3 region (844 bp sequenced) of P. sahandi n. sp. were sequenced for molecular characterization. Sequences of the 18S and 28S D2/D3 of P. sahandi n. sp. have distinct differences from those of the only sequenced P. macrolaimus, with 6 bp differences in 18S and 38 bp differences and five gaps in 28S. This is the first report of the occurrence of members of Actinolaimidae in Iran.

Morphological and molecular characterization of Buzionema lutgardae n. sp. (Nematoda: Oxyuridomorpha: Thelastomatidae) from the cockroach Byrsotria sp. (Blattaria: Blaberidae) in Cuba

Zootaxa ◽  
2021 ◽  
Vol 4965 (2) ◽  
pp. 385-395
Author(s):  
JANS MORFFE ◽  
NAYLA GARCÍA ◽  
KOICHI HASEGAWA ◽  
RAMON A. CARRENO
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Sister Group ◽  
Cylindrical Part ◽  
28S Rdna ◽  
Monophyletic Clade ◽  
Basal Bulb ◽  
Morphological And Molecular Characterization

Buzionema lutgardae n. sp. (Nematoda: Oxyuridomorpha: Thelastomatidae) is described from the cockroach Byrsotria sp. (Blattaria: Blaberidae), endemic to Cuba. Females of B. lutgardae n. sp. are shorter than those of B. validum Kloss, 1966 (1600–2150 µm vs. 3131–3378 µm), but the oesophagus is comparatively longer (b = 2.96–3.77 vs. 4.65–4.87). The lateral alae of the new species extend from ca. the midpoint of the cylindrical part of the procorpus to the level of the anus in contrast to the base of the basal bulb to the level of the anus in B. validum. The males of B. lutgardae n. sp. are shorter than those of B. validum (780–940 µm vs. 1177–1423 µm) and their lateral alae end at some distance before the cloaca instead the level of the cloaca in B. validum. The phylogeny of B. lutgardae n. sp. is inferred by the D2-D3 domains of the 28S rDNA. B. lutgardae n. sp. and B. validum form a monophyletic clade with strong nodal support, as sister-group of the genus Leidynema Schwenck in Travassos, 1929. 

scholarly journals Molecular and morphological characterization of Isthmiophora melis (Schrank, 1788) Luhe, 1909 (Digenea: Echinostomatidae) from American mink (Neovison vison) and European polecat (Mustela putorius) in Lithuania

2017 ◽  
Vol 54 (2) ◽  
pp. 97-104 ◽  
Author(s):  
D. Nugaraitė ◽  
V. Mažeika ◽  
A. Paulauskas
Keyword(s):  
18S Rdna ◽  
American Mink ◽  
Morphological Characterization ◽  
Neovison Vison ◽  
28S Rdna ◽  
Its2 Region ◽  
Mustela Putorius ◽  
Partial 18S

Summary The specimens collected from American mink (Neovison vison) and European polecat (Mustela putorius) in Lithuania were morphologically identified as Isthmiophora melis (Schrank, 1788) Lühe, 1909 and were molecularly characterized through sequencing of partial 18S rDNA, 28S rDNA, ITS1- 5.8S-ITS2 region, and ND1. Relations of I. melis to other species of the genus Isthmiophora Lühe 1909 were discussed. According to ITS1 and ND1 sequences the closest species to I. melis is Isthmiophora hortensis (Asada, 1926).

scholarly journals Trypanosoma irwini n. sp (Sarcomastigophora: Trypanosomatidae) from the koala (Phascolarctos cinereus)

Parasitology ◽  
2009 ◽  
Vol 136 (8) ◽  
pp. 875-885 ◽  
Author(s):  
L. M. McINNES ◽  
A. GILLETT ◽  
U. M. RYAN ◽  
J. AUSTEN ◽  
R. S. F. CAMPBELL ◽  
...  
Keyword(s):  
New Species ◽  
18S Rdna ◽  
Morphological Analysis ◽  
Genetic Characterization ◽  
Clinical Signs ◽  
Trypanosome Species ◽  
Phascolarctos Cinereus ◽  
A New Species

SUMMARYThe morphology and genetic characterization of a new species of trypanosome infecting koalas (Phascolarctos cinereus) are described. Morphological analysis of bloodstream forms and phylogenetic analysis at the 18S rDNA and gGAPDH loci demonstrated this trypanosome species to be genetically distinct and most similar to Trypanosoma bennetti, an avian trypanosome with a genetic distance of 0·9% at the 18S rDNA and 10·7% at the gGAPDH locus. The trypanosome was detected by 18S rDNA PCR in the blood samples of 26 out of 68 (38·2%) koalas studied. The aetiological role of trypanosomes in koala disease is currently poorly defined, although infection with these parasites has been associated with severe clinical signs in a number of koalas. Based on biological and genetic characterization data, this trypanosome species infecting koalas is proposed to be a new species Trypanosome irwini n. sp.

Two new brackish-water species of Pogaina (Rhabdocoela: Provorticidae) from China

Zootaxa ◽  
2019 ◽  
Vol 4679 (2) ◽  
pp. 353-364
Author(s):  
LIN-HONG ZHONG ◽  
YAO-HANG XIE ◽  
XIAO-ZHOU HU ◽  
YU-SI CHEN ◽  
WEI-TAO FENG ◽  
...  
Keyword(s):  
New Species ◽  
Brackish Water ◽  
18S Rdna ◽  
Intertidal Zone ◽  
28S Rdna ◽  
Two New Species ◽  
Brackish Water Species ◽  
Water Species

Two new species of the genus Pogaina Marcus, 1954 (Provorticidae), namely Pogaina sinensis n. sp. and Pogaina shenzhenensis n. sp. are described from brackish water in the intertidal zone of Shenzhen Bay. For P. sinensis n. sp., the tubular stylet is slightly curved at the distal end and a fusiform structure with a flange is present at 66% of the stylet. The flange encircles the stylet, with its ends attached to the midpoint and the distal end of the stylet. For P. shenzhenensis n. sp., the stylet has a N-shaped overall morphology. A band provided with dense needle-like structures is present at the distal end of the stylet. Both the morphological (stylet) and phylogenetic (18S rDNA and 28S rDNA) analyses support the establishment of these two new species. 

Two new species of Kalyptorhynchia (Koinocystididae and Gnathorhynchidae) from China

Zootaxa ◽  
2017 ◽  
Vol 4337 (4) ◽  
pp. 573
Author(s):  
YI-TAO LIN ◽  
WEI-TAO FENG ◽  
JIE-YI ZHUANG ◽  
YU ZHANG ◽  
AN-TAI WANG
Keyword(s):  
New Species ◽  
Brackish Water ◽  
18S Rdna ◽  
Anterior Part ◽  
28S Rdna ◽  
Proboscis Hook ◽  
Dead End ◽  
Two New Species

Two new species of Kalyptorhynchia (Rhabdocoela), namely Itaipusa sinensis n. sp. (Koinocystididae) and Prognathorhynchus sinensis n. sp. (Gnathorhynchidae) were described from brackish water near Shenzhen Bay, representing the first records of Koinocystididae and Gnathorhynchidae in China. For I. sinensis n. sp., the U-shaped cirrus contains 50 triangular lamellar spines, with two cuticular (pseudocuticular) hooks situated at both sides of its anterior part. The hook I (right side) is funnel-shaped, while the hook II (left side) is triangular lamellar in shape. For P. sinensis n. sp., the proboscis hook has two hooks situated in an anterior to posterior sequence. The semicircular tubular stylet has a hollow spherical dead end posteriorly to the distal opening. Both the morphological and phylogenetic (18S rDNA and 28S rDNA) analyses support the establishment of these two new species. 

First record of the genus Sakia Khan, 1964 (Nematoda: Tylenchidae) from Iran, with proposal of Sakia arboris n. sp.

Nematology ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 449-460 ◽  
Author(s):  
Yousef Panahandeh ◽  
Ebrahim Pourjam ◽  
Ali Roshan-Bakhsh ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
First Record ◽  
Small Subunit ◽  
28S Rdna ◽  
Northern Iran ◽  
Body Contour

Summary The genus Sakia is recorded from Iran for the first time and Sakia arboris n. sp., recovered from two geographical points in northern Iran, is described. The new species is characterised by its smooth cuticle under light microscopy (LM), absence of lateral fields, head continuous with body contour, flattened lip region, 9.3 (9.0-10.0) μm long stylet, vulva at 60.2 (59.3-61.3)%, bilobed spermatheca, 117 (102-128) μm long tail, and 14.3 (14-15) μm long spicules. By lacking lateral fields, the new species comes close to three known species of the genus, namely: S. alii, S. castori and S. indica. Molecular phylogenetic analyses using sequences of the near full-length fragment of the small subunit of ribosomal DNA (18S rDNA) and the D2-D3 expansion segments of the large subunit ribosomal DNA (28S rDNA) were performed using Bayesian inference (BI) and maximum likelihood (ML) methods. In the reconstructed Bayesian tree using the 18S rDNA sequence of the type population, the new species occupied a position in a clade including two isolates of Sakia sp. and some species of Lelenchus, with maximal BPP and high ML BS values (1.00/99%). In the reconstructed 28S rDNA phylogenetic tree, two newly sequenced isolates of S. arboris n. sp. formed a well-supported clade with Lelenchus spp.

A new Cosmocercoides species (Ascaridida: Cosmocercidae), C. wuyiensis n. sp., from the Asiatic frog Amolops wuyiensis (Amphibia: Anura)

2019 ◽  
Vol 94 ◽  
Author(s):  
Y. Liu ◽  
Q. Yu ◽  
Y.-L. Shu ◽  
J.-H. Zhao ◽  
J.-Y. Fang ◽  
...  
Keyword(s):  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Nematode Species ◽  
Coi Gene ◽  
Male Body ◽  
Lizard Species ◽  
Nucleotide Divergence ◽  
Distinguishing Features ◽  
Morphological And Molecular Characterization

Abstract We identified and characterized a new cosmocercid nematode species, Cosmocercoides wuyiensis n. sp., through microscopic examination and sequencing of the partial small ribosomal RNA gene (18S rDNA), internal transcribed spacer (ITS) and mitochondrial cytochrome c oxidase subunit 1 (COI) genes. The new species was isolated from the intestine of the Asiatic frog Amolops wuyiensis Liu and Hu, 1975 captured from four localities of the Anhui province in south-east China. Among the 25 recorded species of the Cosmocercoides genus, the morphology of C. wuyiensis n. sp. is closest to that of C. kiliwai and C. malayensis, which were isolated from various Mexican frog and Malaysian lizard species, respectively. However, C. wuyiensis n. sp. displayed several distinguishing features, such as small size of the male body, two spicules of unequal lengths in the male, small gubernaculum, pre-, ad- and post-cloacal caudal rosette papillae in the ratio of 18–24:2:6 and simple papillae in the ratio of 14:multiple:4, circle and number of punctation in each rosette at 1:11–16, sharply conical tail-end and the presence of lateral alae and somatic papillae in both sexes. BLAST and the phylogenetic analyses of the 18S rDNA and ITS sequences indicated that C. wuyiensis n. sp. belonged to the genus Cosmocercoides, while that of the COI gene sequence of C. wuyiensis n. sp. showed 16.36% nucleotide divergence with C. pulcher and 47.99% nucleotide divergence with C. qingtianensis. The morphological and molecular characterization of C. wuyiensis n. sp. provides new taxonomic data for this genus.

Two new species of Rhabdocoela (Polycystididae and Trigonostomidae) from China

Zootaxa ◽  
2019 ◽  
Vol 4695 (4) ◽  
pp. 351-366
Author(s):  
XIAO-ZHOU HU ◽  
YU-SI CHEN ◽  
LIN-HONG ZHONG ◽  
YAO-HANG XIE ◽  
WEI-TAO FENG ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
18S Rdna ◽  
Intertidal Zone ◽  
Copulatory Organ ◽  
28S Rdna ◽  
Muscular Layer ◽  
Similar Length ◽  
Shenzhen City ◽  
Two New Species

Two new species of Rhabdocoela, namely Alcha sinensis n. sp. (Polycystididae) and Trigonostomum sinensis n. sp. (Trigonostomidae), were discovered from the intertidal zone of eastern Shenzhen City, China. For A. sinensis n. sp., the stylet consists of two symmetrical triangular plates and one lamellar plate. All three plates are jagged at their posterior ends. The anterior end of the stylet connects to a thick muscular layer, which causes its movement. For T. sinensis n. sp., the copulatory organ consists of a long-tubular stylet and two "T"-shaped plates (plate I and plate II). The stylet bends 120° at 25% of its length from the base and extends straight distally. Two "T" plates are connected to each other and surround the stylet. Plate I is hook-shaped at its distal end, and plate II has a similar length but only half the width of plate I. The phylogenetic (18S rDNA and 28S rDNA) results also support the establishment of these two new species. On the basis of the molecular phylogeny and morphology of the copulatory organ and bursa appendage, we propose a new categorization of the species of Trigonostomum. 

Morphological and molecular characterization of Klossnema viguerasi n. sp. (Nematoda: Oxyuridomorpha: Hystrignathidae) from a Cuban passalid beetle (Coleoptera: Passalidae), first record of the genus for Cuba

Zootaxa ◽  
2021 ◽  
Vol 4933 (3) ◽  
pp. 391-402
Author(s):  
JANS MORFFE ◽  
NAYLA GARCÍA ◽  
KOICHI HASEGAWA ◽  
KARIN BREUGELMANS
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
18S Rdna ◽  
West Indies ◽  
Tail Length ◽  
First Record ◽  
Generic Diagnosis ◽  
The West ◽  
Morphological And Molecular Characterization

Klossnema viguerasi n. sp. (Nematoda: Oxyuridomorpha: Hystrignathidae) is described from the passalid beetle Antillanax pertyi (Kaup, 1869), endemic to Cuba. The females of K. viguerasi n. sp. are morphologically similar but slightly longer than K. repentina Cordeiro & Artigas, 1983 (1.143 mm vs. 1.000 mm). Both species differ in that K. viguerasi n. sp. has a longer procorpus (139 µm vs. 110 µm), isthmus (39 µm vs. 24 µm), and tail length (28 µm vs. 21 µm). The distance from the vulva to the anterior end is also longer in the new species (0.748 mm vs. 0.650 mm). The males of K. viguerasi n. sp. are larger than K. repentina (0.980 mm vs. 0.800 mm), but their isthmus is shorter (38 µm vs. 48 µm). New features of the cephalic end of both sexes, and copulatory papillae pattern of the males were observed by SEM and the generic diagnosis is emended in order to include such features. The phylogeny of K. viguerasi n. sp. is inferred by the analysis of the D2–D3 domains of the 28S rDNA and the 18S rDNA. This constitutes the first record of the genus Klossnema for the Cuban archipelago and the West Indies. 

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