Production of Volatile Organic Compounds by Trichoderma in Media Containing Different Amino Acids and Their Effect on Selected Wood Decay Fungi

Summary The paper describes an experiment to evaluate the effect of subtle changes to the amino acid composition of the growth medium on the production of volatile organic compounds (VOCs) by a Trichoderma aureoviride isolate and the consequent inhibition of wood decay fungi. A synthetic medium was produced to represent the C:N ratio and major amino acid content of Scots pine sapwood. Changes in the amino acid content of the medium produced very significant differences in the VOC output produced by the Trichoderma isolate and in the levels of inhibition of the target wood decay fungi. Principal component analysis of the VOC profiles indicated that aldehyde and ketone components were associated with the greatest inhibition of the target fungi. The implications of the results for the use of Trichoderma isolates as biological control agents of decay fungi in wood are discussed.

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Analysis of microbial volatile organic compounds produced by wood-decay fungi

Biotechnology Letters ◽

10.1007/s10529-015-1870-9 ◽

2015 ◽

Vol 37 (9) ◽

pp. 1845-1852 ◽

Cited By ~ 15

Author(s):

Rumi Konuma ◽

Kiwamu Umezawa ◽

Atsushi Mizukoshi ◽

Kensuke Kawarada ◽

Makoto Yoshida

Keyword(s):

Volatile Organic Compounds ◽

Organic Compounds ◽

Wood Decay ◽

Decay Fungi ◽

Wood Decay Fungi ◽

Volatile Organic ◽

Microbial Volatile Organic Compounds

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Effect of substrate composition on production of volatile organic compounds from Trichoderma spp. Inhibitory to wood decay fungi

International Biodeterioration & Biodegradation ◽

10.1016/s0964-8305(97)00015-2 ◽

1997 ◽

Vol 39 (2-3) ◽

pp. 199-205 ◽

Cited By ~ 99

Author(s):

Ron Wheatley ◽

Christine Hackett ◽

Alan Bruce ◽

Andrzej Kundzewicz

Keyword(s):

Volatile Organic Compounds ◽

Organic Compounds ◽

Wood Decay ◽

Trichoderma Spp ◽

Decay Fungi ◽

Substrate Composition ◽

Wood Decay Fungi ◽

Volatile Organic

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The Effects of Specific Volatile Organic Compounds Produced by Trichoderma Spp. on the Growth of Wood Decay Basidiomycetes

Holzforschung ◽

10.1515/hf.2001.038 ◽

2001 ◽

Vol 55 (3) ◽

pp. 233-237 ◽

Cited By ~ 20

Author(s):

Sonia N. Humphris ◽

Ron E. Wheatley ◽

Alan Bruce

Keyword(s):

Volatile Organic Compounds ◽

Organic Compounds ◽

Wood Decay ◽

Growth Media ◽

Malt Extract ◽

Trichoderma Spp ◽

Decay Fungi ◽

Respiration Rates ◽

Wood Decay Fungi ◽

Volatile Organic

Summary Previous work by Srinivasan et al. (1992) and Bruce et al. (1996) has shown that inhibition of wood decay fungi by volatile organic compounds produced by Trichoderma spp. is dependent on the type of growth media and age of the Trichoderma colony. Wheatley et al. (1997) identified five volatile organic compounds produced by Trichoderma spp. that may be inhibitory to wood decay fungi. The effects of four of these compounds (Acetone, 2-methyl-1-butanol, heptanal and octanal) were tested over a range of concentrations against four selected wood decay fungi. Fungi were incubated in malt extract broth under appropriate conditions and growth was estimated by biomass production and respiration rates. Growth of all four fungi was affected by at least one of the compounds, usually by inhibition but occasionally stimulation. All but two of the fungus/chemical combinations showed significant effects on biomass development (P < 0.05) and fifteen of the sixteen combinations produced a significant concentration effect on respiration rates (P < 0.05). The aldehydes, heptanal and octanal, were very effective against all four wood decay fungi. All four fungi were inhibited by more than 80% at 25 μg ml−1 by heptanal and three of the four fungi were totally inhibited at 250 μg ml−1. Acetone did not inhibit the four wood decay fungi and even stimulated fungal growth at some concentrations. 2-methyl-1-butanol was only effective at the highest concentration of 2500 μg ml−1. The implications of these results for the biological control of wood decay fungi and future studies are discussed.

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