Development and Optimization of Attapulgite Clay Based Microencapsulation for Lactic Acid Bacteria by Response Surface Methodology

2019 ◽  
Vol 15 (8) ◽  
Author(s):  
Yuping Zhao ◽  
Shuai Liu ◽  
Yunqi Feng ◽  
Muhammad Bilal
Keyword(s):  
Response Surface Methodology ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Response Surface ◽  
Surface Characterization ◽  
Optimal Conditions ◽  
External Gelation ◽  
Major Factors ◽  
Embedding Rate ◽  
Embedding Effect

AbstractLactic acid bacteria (LAB), screened and purified from the fermented yogurt, were microencapsulated in sodium alginate (SA) and attapulgite composite microcapsules by external gelation to increase their viability and stability. Surface characterization by scanning electron microscope clearly evidenced a high number of the LAB embedded in SA/attapulgite composite microcapsules than SA counterparts due to a more cohesive structure, and biocompatible microenvironment. SA/attapulgite and CaCl2/attapulgite composites analysis revealed a better embedding effect of attapulgite blend with SA solvent compared with attapulgite mixed with CaCl2. Influence of three major factors including SA, calcium chloride, and attapulgite concentration on LAB embedding rate were optimized by “single factor strategy” as well as response surface methodology (RSM). Optimal conditions of these factors obtained by RSM were SA (1.03 %), Attapulgite (0.28 %), and CaCl2 concentration (1.17 %). The related embedding rate was predicted as 87.1369 %, and the actual measured value was 91.24 % by experiments using the optimal conditions. In conclusion, the results revealed that LAB microencapsulation in the SA and attapulgite composite might display noteworthy protection against the gastrointestinal environment.

scholarly journals Optimization of Synbiotics of Lactic Acid Bacteria Derived from Kelp Kimchi Using the Response Surface Methodology

2021 ◽  
Vol 50 (12) ◽  
pp. 1385-1391
Author(s):  
Changheon Lee ◽  
Jin Ha Sim ◽  
Jin Hyeon Kim ◽  
Ye-Jun Song ◽  
Eun-Ik Son ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Response Surface

Investigation of Optimal Esterification Conditions of Lactic Acid with Butanol by Using Response Surface Methodology

2021 ◽  
Vol 1020 ◽  
pp. 224-230
Author(s):  
Meng Jun Zheng ◽  
Hsin Chi Tseng ◽  
Bo Yao Chiu ◽  
Wei Cheng Hung ◽  
Richard S. Horng
Keyword(s):  
Response Surface Methodology ◽  
Lactic Acid ◽  
Reaction Time ◽  
Response Surface ◽  
Catalyst Loading ◽  
Esterification Reaction ◽  
Reaction Yield ◽  
Optimal Conditions ◽  
Acid Ratio ◽  
Predicted Values

Esterification reaction of lactic acid with butanol to produce butyl lactate and its optimal conditions were investigated. Cyclohexane was used as entrainer to remove water to promote reaction yield. Catalyst of NaHSO4 was also used to increase reaction rate. Reaction parameters of butanol/lactic acid ratio, cyclohexane/lactic acid ratio, catalyst amount, and reaction time were optimized using Response Surface Methodology (RSM). Results showed that the butanol/lactic acid ratio was the most significant factor for esterification yield while interactions between butanol/lactic acid ratio and cyclohexane/lactic acid ratio, butanol/lactic acid ratio, and reaction time were less significant. The correlation coefficient between predicted values and experiment values was 0.985. The optimal conditions for the experiment are: ethanol/lactic acid ratio 5:1, cyclohexane/lactic acid ratio 1:1, catalyst loading 1.5%, and incubation period 3 hours. The esterification yield reaches 99.8% under these conditions.

Screening of Lactic Acid Bacteria for Anti-Fusarium Activity and Optimization of Incubation Conditions

2017 ◽  
Vol 80 (10) ◽  
pp. 1648-1656 ◽  
Author(s):  
Hui Zhao ◽  
Anuradha Vegi ◽  
Charlene Wolf-Hall
Keyword(s):  
Response Surface Methodology ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Response Surface ◽  
Incubation Time ◽  
Incubation Temperature ◽  
Model Performance ◽  
Inhibition Rate ◽  
Validation Experiment

ABSTRACT Anti-Fusarium activities of lactic acid bacteria (LAB) Lactobacillus plantarum 299V, L. plantarum NRRL-4496, and Lactobacillus rhamnosus VT1 were determined by a microdilution assay developed in this study against Fusarium graminearum 08/RG/BF/51. A cell-free Lactobacillus culture supernatant (CFLCS) of L. rhamnosus VT1 had the highest anti-Fusarium activity. Response surface methodology was used to optimize the incubation conditions for production of CFLCS. A Box-Behnken factorial design was used to investigate the effects of incubation time, shaking speed, and incubation temperature on the inhibition rate of CFLCS. A model equation was generated to predict the inhibition rate of CFLCS under various incubation conditions. A low probability value (0.0012) and associated F value of 25.10 suggested that the model was highly significant. A high R2 value (0.978) indicated a very satisfactory model performance. Response surface methodology analysis suggested that an incubation temperature at 34°C, a shaking speed at 170 rpm, and an incubation time of 55 h were the best combination for production of CFLCS from L. rhamnosus VT1. Under these incubation conditions, a 10% L. rhamnosus VT1 CFLCS solution was predicted to inhibit the growth of F. graminearum by 75.6% in vitro and inhibited 83.7% of the growth in the validation experiment. Thus, the CFLCS of L. rhamnosus VT1 was an effective anti-Fusarium mixture.

scholarly journals Optimasi Molase dan Tibicos sebagai Media Fermentasi dalam Memproduksi Nutraceutical Feed Additive Menggunakan Response Surface Methodology (RSM)

2020 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Mustofa Hilmi ◽  
Anis Usfah Prastujati
Keyword(s):  
Response Surface Methodology ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Response Surface ◽  
Sugar Industry ◽  
Cane Sugar ◽  
Feed Additive ◽  
Growth Media ◽  
Fermentation Time ◽  
Fermentation Parameters

ABSTRAK Molase merupakan hasil samping industri gula tebu yang biasa digunakan dalam proses fermentasi. kandungan molase seperti sukrosa bisa digunakan media pertumbuhan bakteri asam laktat dan yeast sebagai sumber probiotik ternak. Optimasi parameter fermentasi molase memegang peranan yang penting dalam keberhasilan dalam menghasilkan komponen bahan organik, disamping jenis mikroorganisme yang digunakan. Penambahan konsentrasi inokulum (tibicos), konsentrasi molase, dan lama fermentasi menjadi hal yang utama untuk dioptimasi agar bakteri asam laktat dan asam organik  dihasilkan optimal dan waktunya efisien menggunakan response surface methodology (RSM). Metode RSM berguna untuk menentukan nilai-nilai perlakuan (molase, tibicos, lama fermentasi dengan respon optimal terdiri dari 15 perlakuan faktorial bertujuan untuk menentukan variabel tetap dan variabel bebas. Penambahan tibicos dan lama fermentasi berpengaruh signifikan (P<0,05) terhadap jumlah bakteri asam laktat, namun interaksi antara konsentrasi molase dan kosentrasi tibicos (P<0,05) memberikan pengaruh yang signifikan terhadap respon pada tingkat probabilitas 95%. Interaksi kosentrasi tibicos dan lama fermentasi (P<0,05) memberikan pengaruh yang signifikan terhadap jumlah bakteri asam laktat. hasil optimasi, diperoleh nilai optimum kosentrasi molase 12,5%, Kosentrasi tibicos 10% dan waktu fermentasi 36 jam. Kondisi optimum tersebut diprediksi menghasilkan bakteri asam laktat dan prosentasi asam laktat sebesar 7,30333 log CFU/ml dan 0,386667%.Kata kunci: fermentasi, molase, tibicos, RSMABSTRAK Molasses is a by-product of the cane sugar industry which is commonly used in the fermentation process. Molasses such as sucrose can be used as a growth media for lactic acid bacteria and yeast as a source of animal probiotics. Optimization of molasses fermentation parameters plays an essential role in the success in producing components of organic matter, in addition to the types of microorganisms used. The RSM method is useful for determining treatment values (molasses, tibicos, fermentation time with optimal responses consisting of 15 factorial treatments aimed at determining fixed variables and independent variables. The addition of tibicos and fermentation time has a significant effect (P<0.05) on the number of bacteria lactic acid, but the interaction between the concentration of molasses and the concentration of tibicos (P<0.05) has a significant influence on the response at a probability level of 95%. Lactic acid optimization results obtained the optimum value of molasses concentration 12.5%, tibicos concentration 10% and fermentation time of 36 hours. The optimum conditions are predicted to produce lactic acid bacteria and lactic acid percentage of 7.30333 log CFU/ml and 0.386667 %.Keywords: fermentation, molasses, tibicos, RSM

Research on Domestication Process of Lactic Acid Bacteria for Jujube Beverage by Response Surface Methodology

2011 ◽  
Vol 271-273 ◽  
pp. 569-572
Author(s):  
Han Gao ◽  
Ben Guo Liu ◽  
Su Fang Fu
Keyword(s):  
Response Surface Methodology ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Response Surface ◽  
Inoculum Size ◽  
Fermentation Time ◽  
Fermentation Temperature ◽  
Fermented Beverage ◽  
Viable Biomass ◽  
Optimized Conditions

Based on a series of single factor experiments, response surface methodology was employed to predict optimum conditions for domestication of lactic acid bacteria to prepare jujube lactic acid fermented beverage. A central composite design involving jujube puree content, fermentation temperature and inoculum size was used, and second-order model for viable biomass was employed to generate the response surface. The optimized conditions were as follows: jujube puree content 71.85 %, fermentation temperature 41.57°C and inoculum size 3.66%. The predicted viable biomass under the conditions was 3.099×108 cfu/mL. The practical conditions were modified as follows: jujube puree content 72 %, fermentation temperature 41°C, and inoculum size 3.7% (fermentation time 8 h).Experimental verification gave the value of 2.99×108 cfu/mL.

Optimization of the Ultrasonic Extraction of Gypenoside III from Gynostemma pentaphyllum by Response Surface Methodology

2012 ◽  
Vol 550-553 ◽  
pp. 1866-1870
Author(s):  
Xiao Dan Tang ◽  
Hai Yang Hang ◽  
Shao Yan Wang ◽  
Jing Xiang Cong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Gynostemma Pentaphyllum ◽  
Bioactive Component ◽  
Yield Value ◽  
Ultrasonic Time

Gypenosides III is a major bioactive component which is rich in Gynostemma pentaphyllum. For better utilization of the native resource, response surface methodology was used to optimize the extraction conditions of gypenosides III from G. pentaphyllum. The effects of three independent variables on the extraction yield of gypenosides III were investigated and the optimal conditions were evaluated by means of Box-Behnken design. The optimal conditions are as follows: ratio of ethanol to raw material 25, extraction temperature 58°C and ultrasonic time 25min. Under these conditions, the yield of gypenoside III is 1.216±0.05%, which is agreed closely with the predicted yield value.

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