Cytokine changes in gastric and colonic epithelial cell in response to Planta ovata extract

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Javed Yakoob ◽  
Wasim Jafri ◽  
Malik Hassan Mehmood ◽  
Zaigham Abbas ◽  
Kanwal Tariq
Keyword(s):  
Epithelial Cell ◽  
Dna Fragmentation ◽  
Cell Lines ◽  
Inflammatory Reaction ◽  
Sw480 Cell ◽  
Colonic Epithelial Cell ◽  
Ags Cells ◽  
Sw480 Cells

AbstractBackgroundPsyllium (MethodsThe AGS cells and SW480 cells were pretreated with Ispaghul extract in concentrations, i. e. 3.5 and 7 μg/mL prior to infection withResultsDNA fragmentation in AGS and SW480 cells treated with Ispaghul extract was not significant (2.3±0.8 %) compared with untreated cells (2.2±0.6 %). Ispaghul extract decreased theConclusionsIspaghul extract had an effect on stimulated secretion of IL-1β by the AGS and SW480 cell. It decreased pro-inflammatory reaction from both cell lines stimulated by bacteria.

scholarly journals Synergy between Apc min and an activated ras mutation is sufficient to induce colon carcinomas.

1996 ◽  
Vol 16 (3) ◽  
pp. 884-891 ◽  
Author(s):  
G M D'Abaco ◽  
R H Whitehead ◽  
A W Burgess
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Soft Agar ◽  
T Antigen ◽  
Colonic Epithelial Cell ◽  
Large T Antigen ◽  
Ras Gene ◽  
Sv40 Large T Antigen ◽  
Epithelial Cell Lines

Colon carcinomas appear to arise from the cumulative effect of mutations to several genes (APC, DCC, p53, ras, hMLH1, and hMSH2). By using novel colonic epithelial cell lines derived from the Immorto mouse, named the YAMC (young adult mouse colon) cell line, and an Immorto-Min mouse hybrid, named the IMCE (Immorto-Min colonic epithelial) cell line, carrying the Apc min mutation, we investigated the effect of an activated v-Ha-ras gene on tumor progression. The YAMC and IMCE cell lines are normal colonic epithelial cell lines which are conditionally immortalized by virtue of expression of a temperature-sensitive simian virus 40 (SV40) large T antigen. Under conditions which permit expression of a functional SV40 large T antigen (33 degrees C plus gamma interferon), neither the YAMC nor the IMCE cell line grows in soft agar or is tumorigenic in nude mice. In vitro, when the SV40 large T antigen is inactivated (39 degrees C without gamma interferon), the cells stop proliferating and die. By infecting the YAMC and IMCE cell lines with a replication-defective psi2-v-Ha-ras virus, we derived cell lines which overexpress the v-Ha-ras gene (YAMC-Ras and IMCE-Ras). In contrast to the parental cell lines, under conditions in which the SV40 large T antigen is inactive, both the YAMC-Ras and IMCE-Ras cell lines continue to proliferate. Initally YAMC-Ras cells do not form tumors; however, tumors are visible after 90 days of incubation. IMCE-Ras cells form colonies in soft agar under both permissive and nonpermissive culture conditions. Furthermore, IMCE-Ras cells form tumors in nude mice within 3 weeks. The phenotype of the IMCE-Ras cell line thus clearly demonstrates that a defective Apc allele and an activated ras gene are sufficient to transform normal colonic epithelial cells and render them tumorigenic.

The Effect of IL-26 on TNF-α-Induced CXCL8 Responses by Colonic Epithelial Cell Lines

2019 ◽  
Vol 48 (8) ◽  
pp. 822-834
Author(s):  
Isabelle M. Weishaar ◽  
Rebecca S. Young ◽  
Brody M. Wiles ◽  
Dennis W. McGee
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Colonic Epithelial Cell ◽  
Tnf Α ◽  
Epithelial Cell Lines

Lactobacilli survival and adhesion to colonic epithelial cell lines is dependent on long chain fatty acid exposure

2017 ◽  
Vol 119 (11) ◽  
pp. 1700062 ◽  
Author(s):  
Kerry L. Bentley-Hewitt ◽  
Arjan Narbad ◽  
Gosia Majsak-Newman ◽  
Mark R. Philo ◽  
Elizabeth K. Lund
Keyword(s):  
Fatty Acid ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Chain Fatty Acid ◽  
Acid Exposure ◽  
Colonic Epithelial Cell ◽  
Long Chain Fatty Acid ◽  
Epithelial Cell Lines ◽  
Long Chain
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