Effects of quinine on gastric ulcer healing in Wistar rats

2017 ◽  
Vol 14 (4) ◽  
Author(s):  
Olasupo Stephen Adeniyi ◽  
Olubiyi Vincent Makinde ◽  
Emmanuel Titus Friday ◽  
Samuel Babafemi Olaleye
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Superoxide Dismutase Activity ◽  
Mucus Secretion ◽  
Gastric Mucus ◽  
Inflammatory Phase ◽  
Significant Difference

AbstractBackgroundQuinine (QT) is an important anti-malarial drug; however, there is little information about its effects on the gut. Therefore, this study aimed to investigate the effects of a therapeutic dose of QT on the healing of gastric ulcer in rats.MethodsMale Wistar rats weighing 150–200 g were divided into three groups: control rats without ulcer (group 1), ulcerated rats treated with 1 mL/kg (p.o.) normal saline (NS) (group 2), and ulcerated rats treated with 10 mg/kg (p.o.) QT (group 3). Ulcers were induced by serosal application of 80 % acetic acid to the stomach of rats anaesthetized with 50 mg/kg thiopentone sodium and treatment was given three times daily. Healing was assessed on days 3, 7 and 10 after ulcer induction by macroscopic measurement of: ulcer area, histology, lipid peroxidation, superoxide dismutase activity and gastric mucus secretion.ResultsAt day 3, there was no significant difference (p>0.05) in ulcer areas between NS- and QT-treated rats. By day 10, however, the percentage area healed in NS treated (59.6±2.35 %) was significantly higher (p<0.05) than in QT rats (49.0±2.20 %) and clearing of inflammatory cells and re-epithelization was greater in NS-treated group. By days 7 and 10, lipid peroxidation was significantly higher in QT animals, when compared with NS-treated rats and controls (p<0.05). Superoxide dismutase activity and mucus secretion were significantly (p<0.05) higher in NS-treated than QT-treated rats.ConclusionsQT delayed ulcer healing by prolonging the inflammatory phase of healing, increasing oxidative stress, reducing antioxidant activity and gastric mucus secretion

scholarly journals Lipid Peroxidation, Erythrocyte Superoxide-Dismutase Activity and Trace Metals in Young Male Footballers

2003 ◽  
Vol 44 (6) ◽  
pp. 979 ◽  
Author(s):  
Gökhan Metin ◽  
Pınar Atukeren ◽  
A. Ata Alturfan ◽  
Tevfik Gülyaşar ◽  
Mehmet Kaya ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Trace Metals ◽  
Young Male ◽  
Superoxide Dismutase Activity ◽  
Erythrocyte Superoxide Dismutase

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

ESSENTIAL OILS SUPEROXIDE DISMUTASE ACTIVITY AND LIPID PEROXIDATION IN OCIMUM BASILICUM L.

1993 ◽  
pp. 347-351
Author(s):  
D. Å tajner ◽  
O. GaÅ¡ic ◽  
E. Lemberkovics ◽  
I. Mathe ◽  
N. Mimica-Dukic
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Essential Oils ◽  
Ocimum Basilicum ◽  
Superoxide Dismutase Activity

Superoxide dismutase activity and the effects of NBQX and CPP on lipid peroxidation in experimental spinal cord injury

1999 ◽  
Vol 199 (5) ◽  
pp. 285-293 ◽  
Author(s):  
Aşkın Görgülü ◽  
Talat Kırış ◽  
Faruk Ünal ◽  
Ümit Turkoğlu ◽  
Mutlu Küçük ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Lipid Peroxidation ◽  
Spinal Cord Injury ◽  
Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
Experimental Spinal Cord Injury ◽  
Cord Injury

scholarly journals Prooxidant-Antioxidant Balance in the Heart of Rats with Different Behavioral Activities under the Conditions of Light Exposition

2021 ◽  
Vol 6 (3) ◽  
pp. 360-365
Author(s):  
L. D. Chebotar ◽  
◽  
E. N. Laricheva ◽  
M. Sh. Gilmutdinova
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Open Field ◽  
Emotional Stress ◽  
Light Exposure ◽  
Superoxide Dismutase Activity ◽  
Main Role ◽  
Experimental Animals ◽  
Behavioural Activity

The purpose of the article. The article shows that the effect of round-the-clock lighting causes changes in the processes of lipid peroxidation and antioxidant activity in rats, and depends on their behavioural activity. The effects of lighting on the processes of oxidative metabolism of varying degrees in the heart of resistant and unstable to emotional stress rats. Materials and methods. Investigations were carried out on 32 mature Wistar rats, divided into four groups: group 1 – animals resistant to emotional stress (intact); group 2 – intact animals unstable to emotional stress; group 3 – experimental animals resistant to emotional stress (30-day light exposure (1000 lux)); group 4 – experimental animals, unstable to emotional stress (30-day light exposure (1000 lux)). When assessing the effect of light on the state of the organism, the most important integral indicator is the behavior of animals. Therefore, during the experiment we used the observation of behavioral reactions in the test “open field”. Based on the characteristics of the behavior of animals in the “open field” rats were divided into groups resistant and unstable to emotional stress. To assess lipid peroxidation in the heart homogenate, the concentration of TBA-active products, the concentration of diene, oxidiene and triene conjugates were determined. Antioxidant processes were assessed by the increase in the concentration of TBA- active products during 1.5-hour incubation in an iron-ascorbate buffer solution, as well as by the activity of superoxide dismutase and catalase. Results and discussion. Prooxidant activity was characterized by an increase in the concentration of TBA-reactive substances in animals resistant to emotional stress. The concentration of TBA-reactive substances after 1.5-hour incubation increased in both experimental groups. Changes in the antioxidant status were illustrated by an increase in superoxide dismutase activity in the group of stress-unstable rats, whereas catalase activity increased in both experimental groups. In addition, in the group of animals resistant to emotional stress, a significant decrease in the resources of α-tocopherol and β-carotene was revealed. Conclusion. The long-term light exposure promotes the formation of end products of peroxidation in the heart of rats resistant to emotional stress and causes a decrease in antioxidant potential, regardless of behavioural activity. Antioxidant activity in the heart of emotionally stress-resistant rats is realized through both the enzyme and non-enzyme links of the antioxidant defence, while the main role in the heart of emotionally stress-resistant rats is played by superoxide dismutase activity

Gastro-protective effects of camel milk on indomethacin-induced peptic ulcer in Wistar rats

2018 ◽  
Vol 7 (2) ◽  
pp. 42-56
Author(s):  
Mansouria Belhocine ◽  
Abdelkader Homrani ◽  
Fatima Azzouz Azzouz ◽  
Chahrazed Sakmeche
Keyword(s):  
Antioxidant Activity ◽  
Gastric Ulcer ◽  
Blood Cells ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Camel Milk ◽  
Control Group ◽  
Gastric Mucus ◽  
Ulcer Index

Gastric ulcer associated with the use of nonsteroidal anti-inflammatory drugs (NSAIDs) and low-dose aspirin is a major public health problem. The present study was undertaken to determine the camel milk antioxidant activity, tested on DPPH, and its gastro-protective effect, investigated in Wistar rats sub-jected to gastric ulcer induced by a nonsteroidal anti-inflammatory agent, indomethacin. The study was performed on 20 adult male Wistar rats divided into 4 groups of 5 rats each. The negative control group received distilled water, the positive control group received indomethacin, the standard group received ranitidine and the fourth group was pretreated with raw camel milk, for 15 days respectively. On the 16th day, the indomethacin was ad-ministered to all rats except those of the negative control group. The ulcerogenic effect of indomethacin was highly significant, evidenced by a large number of ulcer lesions, a remarkably high ulcer index, and an important decrease in adherent gastric mucus. Camel milk resulted in significant gastro-protection compared to indomethacin ulcerated rats as manifested by significant decrease in ulcerative lesions number, and the ulcer index with a restored gastric mucus wall. The camel milk protection percentage is close to that of ranitidine. Additionally, in indomethacin-injured rats an increase in white blood cells, granulocytes, serum transaminases, and hemoglobin levels with a lowering in red blood cells were reported. These physiological disturbances were recovered by camel milk. Camel milk seemed to have gastro-protective effect, probably through its strong antioxidant activity, and may be recommended to patients with arthritis.

Sign in / Sign up

Export Citation Format

Share Document