scholarly journals LmTDRM Database: A Comprehensive Database on Thiol Metabolic Gene/Gene Products in Listeria monocytogenes EGDe

2014 ◽  
Vol 11 (1) ◽  
pp. 17-29
Author(s):  
Vanishree Srinivas ◽  
Shubha Gopal
Keyword(s):  
Oxidative Stress ◽  
Listeria Monocytogenes ◽  
Protein Function ◽  
Model Organism ◽  
Reducing Power ◽  
Major Step ◽  
Redox Metabolism ◽  
Interaction Database ◽  
Insertion Elements ◽  
Thiol Redox

Summary There are a number of databases on the Listeria species and about their genome. However, these databases do not specifically address a set of network that is important in defence mechanism of the bacteria. Listeria monocytogenes EGDe is a well-established intracellular model organism to study host pathogenicity because of its versatility in the host environment. Here, we have focused on thiol disulphide redox metabolic network proteins, specifically in L. monocytogenes EGDe. The thiol redox metabolism is involved in oxidative stress mechanism and is found in all living cells. It functions to maintain the thiol disulphide balance required for protein folding by providing reducing power. Nevertheless, they are involved in the reversible oxidation of thiol groups in biomolecules by creating disulphide bonds; therefore, the term thiol disulphide redox metabolism (TDRM). TDRM network genes play an important role in oxidative stress mechanism and during host-pathogen interaction. Therefore, it is essential to have detailed information on these proteins with regard to other bacteria and its genome analysis to understand the presence of tRNA, transposons, and insertion elements for horizontal gene transfer. LmTDRM database is a new comprehensive web-based database on thiol proteins and their functions. It includes: Description, Search, TDRM analysis, and genome viewer. The quality of these data has been evaluated before they were aggregated to produce a final representation. The web interface allows for various queries to understand the protein function and their annotation with respect to their relationship with other bacteria. LmTDRM is a major step towards the development of databases on thiol disulphide redox proteins; it would definitely help researchers to understand the mechanism of these proteins and their interaction. Database URL: www.lmtdrm.com

scholarly journals Characterization of cellular oxidative stress response by stoichiometric redox proteomics

2020 ◽  
Author(s):  
Tong Zhang ◽  
Matthew J. Gaffrey ◽  
Xiaolu Li ◽  
Wei-Jun Qian
Keyword(s):  
Oxidative Stress ◽  
Posttranslational Modifications ◽  
Protein Function ◽  
Regulatory Networks ◽  
Total Oxidation ◽  
Redox Proteomics ◽  
Thiol Redox ◽  
Cysteine Thiols ◽  
In Cells

The thiol redox proteome refers to all proteins whose cysteine thiols are subjected to various redox-dependent posttranslational modifications (PTMs) including S-glutathionylation (SSG), S-nitrosylation (SNO), S-sulfenylation (SOH), and S-sulfhydration (SSH). These modifications can impact various aspects of protein function such as activity, binding, conformation, localization, and interactions with other molecules. To identify novel redox proteins in signaling and regulation, it is highly desirable to have robust redox proteomics methods that can provide global, site-specific, and stoichiometric quantification of redox PTMs. Mass spectrometry (MS)-based redox proteomics has emerged as the primary platform for broad characterization of thiol PTMs in cells and tissues. Herein we review recent advances in MS-based redox proteomics approaches for quantitative profiling of redox PTMs at physiological or oxidative stress conditions and highlight some recent applications. Considering the relative maturity of available methods, emphasis will be on two types of modifications: 1) total oxidation (i.e., all reversible thiol modifications), the level of which represents the overall redox state, and 2) S-glutathionylation, a major form of reversible thiol oxidation. We also discuss the significance of stoichiometric measurements of thiol PTMs as well as future perspectives towards a better understanding of cellular redox regulatory networks in cells and tissues

scholarly journals NNT Regulates Mitochondrial Metabolism in NSCLC Through Maintenance of Fe-S Protein Function

2019 ◽  
Author(s):  
Nathan P. Ward ◽  
Yun Pyo Kang ◽  
Aimee Falzone ◽  
Terry A. Boyle ◽  
Gina M. DeNicola
Keyword(s):  
Oxidative Stress ◽  
Protein Function ◽  
Lung Tumor ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Reducing Power ◽  
Tumor Formation ◽  
Mitochondrial Metabolism ◽  
Mitochondrial Activity ◽  
Ros Generation ◽  
Pulmonary Tissue

AbstractHuman lung tumors exhibit robust and complex mitochondrial metabolism, likely precipitated by the highly oxygenated nature of pulmonary tissue. As ROS generation is a byproduct of this metabolism, reducing power in the form of nicotinamide adenine dinucleotide phosphate (NADPH) is required to mitigate oxidative stress in response to this heightened mitochondrial activity. Nicotinamide nucleotide transhydrogenase (NNT) is known to sustain mitochondrial antioxidant capacity through the generation of NADPH, however its function in non-small cell lung cancer (NSCLC) has not been established. We found that NNT expression significantly enhances tumor formation and aggressiveness in mouse models of lung tumor initiation and progression. We further show that NNT loss elicits mitochondrial dysfunction independent of substantial increases in oxidative stress, but rather marked by the diminished activities of proteins dependent on resident iron-sulfur clusters. These defects were associated with both NADPH availability and ROS accumulation, suggesting that NNT serves a specific role in mitigating the oxidation of these critical protein cofactors.

σB Plays a Limited Role in the Ability of Listeria monocytogenes Strain F2365 To Survive Oxidative and Acid Stress and in Its Virulence Characteristics

2013 ◽  
Vol 76 (12) ◽  
pp. 2079-2086 ◽  
Author(s):  
H. F. OLIVER ◽  
R. H. ORSI ◽  
M. WIEDMANN ◽  
K. J. BOOR
Keyword(s):  
Oxidative Stress ◽  
Listeria Monocytogenes ◽  
Acid Stress ◽  
Model Organism ◽  
Soft Cheese ◽  
Response To Stress ◽  
Serotype 4B ◽  
And Oxidative Stress

Listeria monocytogenes strain F2365 was the first strain representative of serotype 4b (lineage I) to be sequenced in 2004, suggesting it could become the model organism for this serotype, which is associated with most human outbreaks of listeriosis worldwide to date. F2365 itself is an outbreak strain that was involved in the listeriosis outbreak associated with Mexican-style soft cheese in California in 1985. In this study, we show through phenotypic and transcriptomic analysis that L. monocytogenes strain F2365 has reduced ability to respond to acid and oxidative stress. F2365 has neither the σB-dependent ability to survive acid or oxidative stress nor the σB-dependent ability to infect Caco-2 epithelial cells in vitro or guinea pigs in vivo. More studies are needed to determine whether the atypical σB-independent response to stress observed in F2365 is strain specific, serotype specific, or even lineage specific.

Comparative proteomics profiling of a gentamicin-attenuated Leishmania infantum cell line identifies key changes in parasite thiol-redox metabolism

2012 ◽  
Vol 75 (5) ◽  
pp. 1463-1471 ◽  
Author(s):  
Hamid Daneshvar ◽  
Susan Wyllie ◽  
Stephen Phillips ◽  
Paul Hagan ◽  
Richard Burchmore
Keyword(s):  
Cell Line ◽  
Leishmania Infantum ◽  
Comparative Proteomics ◽  
Redox Metabolism ◽  
Thiol Redox

Listeria monocytogenes: silage, sandwiches and science

2005 ◽  
Vol 6 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Charles J. Czuprynski
Keyword(s):  
Listeria Monocytogenes ◽  
Murine Model ◽  
Pathogenic Bacteria ◽  
Model Organism ◽  
Intracellular Pathogen ◽  
Innate And Adaptive Immunity ◽  
Potential Vector ◽  
Broad Array ◽  
Common Framework ◽  
Substantial Risk

AbstractListeria monocytogenesis amongst the most intriguing and well studied of the pathogenic bacteria. However, the understanding and perspective one has ofL. monocytogenesdepends to a large extent on the microbiological issues with which one is faced as a part of your professional duties. The focus of the veterinary clinician or investigator is likely to be foremost on the neurologic (circling disease) and reproductive diseasesL. monocytogenescauses. To the food microbiologist, the principal concern is to prevent introduction ofL. monocytogenesinto food products, or to identify its presence and prevent its multiplication to numbers of organisms that are likely to pose a substantial risk to humans who ingest the product. To the cellular immunologist, listeriosis represents a robust murine model that helped to elucidate many important concepts in innate and adaptive immunity, andL. monocytogenesis a potential vector for delivery of novel vaccines. To the student of molecular pathogenesis,L. monocytogenesis a powerful and well-characterized model organism for studying the cellular microbiology of an intracellular pathogen. In this brief overview, I will attempt to highlight some of the classical observations, and contemporary insights, onL. monocytogenesand listeriosis, and integrate these perspectives into a common framework. By so doing, I hope to provide those with one perspective on listeriosis with an appreciation of the broad array of problems and issues faced by those who focus on some other aspect ofL. monocytogenesand its pathogenesis.

scholarly journals An Effective Counterselection System for Listeria monocytogenes and Its Use To Characterize the Monocin Genomic Region of Strain 10403S

2016 ◽  
Vol 83 (6) ◽  
Author(s):  
Tal Argov ◽  
Lev Rabinovich ◽  
Nadejda Sigal ◽  
Anat A. Herskovits
Keyword(s):  
Listeria Monocytogenes ◽  
Point Mutations ◽  
Model Organism ◽  
Trna Synthetase ◽  
Genomic Region ◽  
Selection Marker ◽  
Content Type ◽  
Allelic Exchange ◽  
Suicide Vector ◽  
Screening Systems

ABSTRACT Construction of Listeria monocytogenes mutants by allelic exchange has been laborious and time-consuming due to lack of proficient selection markers for the final recombination event, that is, a marker conveying substance sensitivity to the bacteria bearing it, enabling the exclusion of merodiploids and selection for plasmid loss. In order to address this issue, we engineered a counterselection marker based on a mutated phenylalanyl-tRNA synthetase gene (pheS*). This mutation renders the phenylalanine-binding site of the enzyme more promiscuous and allows the binding of the toxic p-chloro-phenylalanine analog (p-Cl-phe) as a substrate. When pheS* is introduced into L. monocytogenes and highly expressed under control of a constitutively active promoter, the bacteria become sensitive to p-Cl-phe supplemented in the medium. This enabled us to utilize pheS* as a negative selection marker and generate a novel, efficient suicide vector for allelic exchange in L. monocytogenes. We used this vector to investigate the monocin genomic region in L. monocytogenes strain 10403S by constructing deletion mutants of the region. We have found this region to be active and to cause bacterial lysis upon mitomycin C treatment. The future applications of such an effective counterselection system, which does not require any background genomic alterations, are vast, as it can be modularly used in various selection systems (e.g., genetic screens). We expect this counterselection marker to be a valuable genetic tool in research on L. monocytogenes. IMPORTANCE L. monocytogenes is an opportunistic intracellular pathogen and a widely studied model organism. An efficient counterselection marker is a long-standing need in Listeria research for improving the ability to design and perform various genetic manipulations and screening systems for different purposes. We report the construction and utilization of an efficient suicide vector for allelic exchange which can be conjugated, leaves no marker in the bacterial chromosome, and does not require the use of sometimes leaky inducible promoters. This highly efficient genome editing tool for L. monocytogenes will allow for rapid sequential mutagenesis, introduction of point mutations, and design of screening systems. We anticipate that it will be extensively used by the research community and yield novel insights into the diverse fields studied using this model organism.

scholarly journals Ultrasonic Cleaning of Conveyor Belt Materials Using Listeria monocytogenes as a Model Organism

2007 ◽  
Vol 70 (3) ◽  
pp. 758-761 ◽  
Author(s):  
RIINA TOLVANEN ◽  
JANNE LUNDÉN ◽  
HANNU KORKEALA ◽  
GUN WIRTANEN
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Model Organism ◽  
Conveyor Belt ◽  
Difficult Problem ◽  
Cleaning Efficiency ◽  
Ultrasonic Cleaning ◽  
Plastic Materials ◽  
Significant Difference ◽  
Logarithmic Reduction

Persistent Listeria monocytogenes contamination of food industry equipment is a difficult problem to solve. Ultrasonic cleaning offers new possibilities for cleaning conveyors and other equipment that are not easy to clean. Ultrasonic cleaning was tested on three conveyor belt materials: polypropylene, acetal, and stainless steel (cold-rolled, AISI 304). Cleaning efficiency was tested at two temperatures (30 and 45°C) and two cleaning times (30 and 60 s) with two cleaning detergents (KOH, and NaOH combined with KOH). Conveyor belt materials were soiled with milk-based soil and L. monocytogenes strains V1, V3, and B9, and then incubated for 72 h to attach bacteria to surfaces. Ultrasonic cleaning treatments reduced L. monocytogenes counts on stainless steel 4.61 to 5.90 log units; on acetal, 3.37 to 5.55 log units; and on polypropylene, 2.31 to 4.40 log units. The logarithmic reduction differences were statistically analyzed by analysis of variance using Statistical Package for the Social Sciences software. The logarithmic reduction was significantly greater in stainless steel than in plastic materials (P < 0.001 for polypropylene, P = 0.023 for acetal). Higher temperatures enhanced the cleaning efficiency in tested materials. No significant difference occurred between cleaning times. The logarithmic reduction was significantly higher (P = 0.013) in cleaning treatments with potassium hydroxide detergent. In this study, ultrasonic cleaning was efficient for cleaning conveyor belt materials.

Comparison of the Stress Response of Listeria monocytogenes Strains with Sprout Colonization

2008 ◽  
Vol 71 (8) ◽  
pp. 1556-1562 ◽  
Author(s):  
LISA GORSKI ◽  
DENISE FLAHERTY ◽  
JESSICA M. DUHÉ
Keyword(s):  
Oxidative Stress ◽  
Statistical Analysis ◽  
Listeria Monocytogenes ◽  
Stress Response ◽  
Foodborne Pathogen ◽  
Acid Stress ◽  
Broccoli Sprouts ◽  
Alfalfa Sprouts ◽  
Different Strains ◽  
And Oxidative Stress

Twenty-nine strains of the foodborne pathogen Listeria monocytogenes were tested for their ability to colonize alfalfa, radish, and broccoli sprouts and their capacity to withstand acid and oxidative stress, two stresses common to the sprouting environment. Wide variation in the ability of different strains to colonize alfalfa sprouts were confirmed, but the variations among radish and broccoli sprouts were not as large. With a few exceptions, strains that were poor colonizers of alfalfa tended to be among the poorer colonizers of radish and broccoli and vice versa. The strains also were variable in their resistance to both acid and oxidative stress. Statistical analysis revealed no correlation between acid stress and sprout colonization, but there was a positive correlation between resistance to oxidative stress and colonization of all three sprout types. Although the response to oxidative stress is important for L. monocytogenes virulence, it also may be important for life outside of a host.

Sign in / Sign up

Export Citation Format

Share Document