scholarly journals Oxidative stress and antioxidant defence markers in muscle tissue of rainbow trout (Oncorhynchus mykiss) after vaccination against Yersinia ruckeri

2016 ◽  
Vol 60 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Halyna Tkachenko ◽  
Joanna Grudniewska ◽  
Agnieszka Pękala ◽  
Elżbieta Terech-Majewska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Muscle Tissue ◽  
Yersinia Ruckeri ◽  
Antioxidant Defence ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference ◽  
Derivatives Of

AbstractIntroduction: The goal of this study was to assess the influence of vaccination against enteric redmouth disease on oxidative stress biomarkers and antioxidant defence in the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against Yersinia ruckeri in the first and second month after immunisation. Material and Methods: Healthy fish were vaccinated orally with inactivated whole cells of a virulent strain of Y. ruckeri. One and two months after immunisation the muscle samples were collected. Results: No significant difference was noted in lipid peroxidation level in either the first or second month after vaccination, while aldehydic and ketonic derivatives of oxidatively modified proteins (OMB) in the vaccinated group were significantly lower in the second month compared to those in the first month after vaccination (P < 0.05). The content of ketonic derivatives of OMB in muscles in the first month after immunisation was higher compared to untreated control. All these culminated in a depletion of glutathione peroxidase (GPx) activity and low level of total antioxidant capacity (TAC). Conclusion: Correlations between catalase activity and lipid peroxidation and TAC confirmed the pivotal role of catalase in antioxidant defence during immunisation. From a broader perspective, it is suggested that immunisation of fish with Yersinia vaccine is associated with induced free radical formation and oxidative stress. Free radicals would therefore be at least partially responsible for the induction of both humoral and cellular elements of the immunity and increased protective immunity against Y. ruckeri infection.

scholarly journals POST-VACCINATED ALTERATIONS IN THE MARKERS OF LIPID AND PROTEIN OXIDATION IN THE GILLS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM) IMMUNIZED AGAINST THE ENTERIC REDMOUTH DISEAS

2020 ◽  
pp. 24-35
Author(s):  
Halyna Tkachenko ◽  
Natalia Kurhaluk ◽  
Joanna Grudniewska ◽  
Agnieszka Pękala-Safińska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Oxidative Stress Biomarkers ◽  
Enzymatic Assays ◽  
Stress Biomarkers ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Derivatives Of ◽  
Isolation Buffer

The aim of the study was the evaluation of the content of oxidative stress biomarkers (2-thiobarbituric-acid-reacting substances as a biomarker of lipid peroxidation, aldehydic and ketonic derivatives of oxidatively modified proteins) in the gills of rainbow trout (Oncorhynchus mykissWalbaum) vaccinated by a vaccine against Yersiniaruckeri. Rainbow trout (Oncorhynchus mykiss Walbaum) with a mean body mass of (107.9±3.1) g were used in the experiments. The study was carried out in a Department of Salmonid Research, Inland Fisheries Institute in Rutki (Poland). Experiments were performed at a water temperature of 14.5±0.5°C and the pH was 7.5. The dissolved oxygen level was about 12 ppm with additional oxygen supply with a water flow of 25 L per min, a photoperiod of 12 hours per day. The fish were fed with a commercial pelleted diet at an optimal level, using 12-hour belt feeders for fish. All enzymatic assays were carried out at the Department of Zoology and Animal Physiology, Institute of Biology and Earth Sciences, Pomeranian University in Słupsk (Poland).The fish were kept for 60 days after vaccination at a water temperature of 14.5±0.5°C and pH 7.5. In our study, 15 rainbow trout from unhandled control and 15 vaccinated trout were used. Two months after immunization, samples from rainbow trout were collected. The fish were captured and killed 61 days post-vaccination (n = 15 in each group). Gills were removed in situ. The organs were rinsed clear of blood with cold isolation buffer and homogenized using a glass homogenizer H500 with a motor-driven pestle immersed in an ice water bath to yield a homogenate in proportion 1:9 (weight/volume). The isolation buffer contained 100 mMTris-HCl; a pH of 7.2 was adjusted with HCl. Homogenates were centrifuged at 3,000g for 15 min at 4°C. After centrifugation, the supernatant was collected and frozen at −20°C until analyzed. Protein contents were determined using the method of Bradford (1976) with bovine serum albumin as a standard. Absorbance was recorded at 595 nm. All enzymatic assays were carried out at 22±0.5°C using a Specol 11 spectrophotometer (Carl Zeiss Jena, Germany) in duplicate. The enzymatic reactions were started by the addition of the tissue supernatant. Our results demonstrated that immunization by the anti-Yersinia vaccine does not alter the gills of rainbow trout. Oxidative stress parameters examined in gills homogenate, i.e., lipid peroxidation as measured by the amount of TBARS, as well as aldehydic (increased by 18.9%) and ketonic derivatives of OMP (decreased by 6.5 %) were non-significantly changed (p>0.05) in gills of vaccinated fish. Thus, immunization by anti-Yersinia vaccine does not alter oxidative stress markers compared to unhandled control in the second month after immunization. Our results confirm that the vaccine against Y. ruckeri has no adverse effect on the condition and metabolism in the gills of the fish. Alterations in the content of oxidative stress biomarkers recorded in our studies are proof that the vaccine against Y. ruckeri has no negative effects.

scholarly journals The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2)

2021 ◽  
Vol 38 (3) ◽  
pp. 269-273
Author(s):  
Mehmet Reşit Taysı ◽  
Muammer Kırıcı ◽  
Mahinur Kırıcı ◽  
Hasan Ulusal ◽  
Bünyamin Söğüt ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Liver Tissue ◽  
Stress Index ◽  
Mercury Chloride ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Total Antioxidant ◽  
Ld50 Value

The aim of this study was to determine oxidative stress caused by mercury chloride (HgCl2) in rainbow trout (Oncorhynchus mykiss) liver tissue. For this purpose, the LD50 value of HgCl2 on rainbow trout was determined as 551 μg/L. In the study, 40 fish in four groups were exposed to 25% and 50% (138 and 276 µg/L) of the two subletal doses of HgCl2 for 2 and 7 days, with 10 fish (n=10) in each group. To determine oxidative stress; peroxynitrite (ONOO−), total oxidant level (TOS), total antioxidant level (TAS), oxidative stress index (OSI) and malondialdehyde (MDA) were analyzed. In the study, it was observed that the differences between the groups in terms of ONOO−, TOS, TAS and OSI levels in the liver tissues was significant (P<0.05), however, this difference was not significant (P>0.05) in terms of MDA values. As a result, it can be concluded that HgCl2 increases ONOO−, TOS, TAS, OSI and MDA levels in liver tissue and even small doses of mercury are toxic to fish.

scholarly journals Immune Response of Rainbow Trout (Oncorhynchus mykiss) to Selected Antigens of Yersinia ruckeri

2009 ◽  
Vol 78 (1) ◽  
pp. 145-150 ◽  
Author(s):  
Unal Ispir ◽  
H. Bayram Gokhan ◽  
Mikail Ozcan ◽  
Mustafa Dorucu ◽  
Naim Saglam
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Metabolic Activity ◽  
Control Fish ◽  
Phagocytic Index ◽  
Control Group ◽  
Yersinia Ruckeri ◽  
Whole Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Fish Group

In this study, effects of Yersinia ruckeri antigens on the immune mechanisms of rainbow trout (Oncorhynchus mykiss) were examined. The weight of the 120 fish used in this study was 20–30 g. After injecting 1 mg of formalin-inactivated whole cells (FKC) and O-antigen (Ag-O) intraperitoneally, blood was taken from the caudal vein of anaesthetized fish and metabolic activity of leukocytes (Nitroblue tetrazolium (NBT) activities), phagocytic activity (PA), phagocytic index (PI), serum protein and serum total immunoglobulin (TIg) levels were determined on day 30 after the first immunization. The same procedure was conducted in the control group. In all the experimental groups, considerable increases in the immune indicators were found and significant differences detected between the control and experimental groups (p < 0.05). Metabolic activity of leukocytes decreased significantly (p < 0.05) during the following treatment with antigens compared to the control fish group.

scholarly journals ANTIOXIDANT ACTIVITY OF VEGETATIVE ORGANS OF DENDROBIUM PARISHII RCHB.F. IN THE MUSCLE TISSUE OF RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM): IN VITRO MODEL STUDY

2020 ◽  
pp. 9-20
Author(s):  
Lyudmyla Buyun ◽  
Oleksandr Gyrenko ◽  
Maryna Opryshko ◽  
Lyudmyla Kovalska ◽  
Halyna Tkachenko ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Antioxidant Capacity ◽  
Muscle Tissue ◽  
Vegetative Organs ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Total Antioxidant ◽  
Oxidatively Modified ◽  
Derivatives Of ◽  
Oxidatively Modified Proteins

This research aimed to evaluate the in vitro effect of buffer extract obtained from leaves and pseudobulbs (modified shoots) of Dendrobium parishii Rchb. f. on the 2-thiobarbituric acid reactive substances (TBARS) as lipid peroxidation biomarker, aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity (TAC) in the muscle tissue of the rainbow trout (Oncorhynchus mykiss Walbaum). The shoots (pseudobulbs) with leaves of Dendrobium parishii cultivated under glasshouse conditions were sampled at M.M. Gryshko National Botanic Garden (NBG) (Kyiv, Ukraine). Since 1999, the whole collection of tropical and subtropical plants (including orchids) has had the status of a National Heritage Collection of Ukraine and is supported through State funding. Besides, NBG’s collection of tropical orchids was registered at the Administrative Organ of CITES in Ukraine (Ministry of Environment Protection, registration No. 6939/19/1-10 of 23 June 2004). The collected pseudobulbs and leaves were brought into the laboratory for biochemical studies. Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in proportion 1:19, w/w) at room temperature. The extract was then filtered and investigated for its antioxidant capacity. The extract was stored at -20°C until use. The increase in TBARS level in the muscle tissue exposed to extracts derived from leaves and pseudobulbs of D. parishii was insignificant. The level of ketonic derivatives of oxidatively modified proteins was non-significantly decreased both for leaf and pseudobulb extracts compared to the untreated samples. The extracts obtained from leaves and pseudobulbs of D. parishii significantly increased the TAC level in muscle tissue due to inhibited the Fe2+/ascorbate-induced oxidation of Tween 80. Overall, these findings demonstrate that aqueous extracts of vegetative organs of Dendrobium parishii can enhance the total antioxidant capacity in the muscle tissue of the rainbow trout. Moreover, this antioxidant effect was more intensive for pseudobulb extracts.

scholarly journals Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

2012 ◽  
Vol 48 (No. 8) ◽  
pp. 215-220 ◽  
Author(s):  
I. Strunjak-Perovic ◽  
R. Coz-Rakovac ◽  
N. Topic Popovic
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Water Temperature ◽  
Peripheral Circulation ◽  
Ploidy Levels ◽  
Mean Values ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference

The aim of the study was to observe the influence of different ploidy levels in fish on micronucleus occur&shy;rence. Twenty minutes after fertilization, one group of rainbow trout eggs was exposed to water temperatures of 26&deg;C in duration of 20 minutes to induce triploidy. Second group was kept in water temperature of 10&deg;C, which is optimal for development of rainbow trout. The frequency of micronucleated erythrocytes was determined in the peripheral circulation of rainbow trout 67 days (following absorption of the yolk &ndash; swim-up stage) and 128 days (fry stage) post fertilization. There was a significant difference (P &lt; 0.001) between frequency of micronucleated erythrocytes of diploid (1.10 &plusmn; 0.96&permil;) and triploid (2.41 &plusmn; 1.28&permil;) fish at swim-up stage. Increased mean values of micronucleus in diploid (1.80 &plusmn; 1.57&permil;) and triploid (5.92 &plusmn; 3.80&permil;) fry were also recorded.

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