Maned wolf (Chrysocyon brachyurus) minimum population size and genetic diversity in a Cerrado protected area of southeastern Brazil revealed by fecal DNA analysis

Abstract Jaguar populations have been declining in Brazil mostly due to habitat loss and fragmentation, conflict with humans, poaching and reduction of prey. This is dramatically true in the Atlantic Forest, where occurrence of this large felid is now restricted to very few remaining areas. We used a non-invasive DNA analysis to search through felid scats collected in the Santa Virginia Unit (SVU) of the Serra do Mar State Park, to test for the potential presence of jaguar there. Our results indicated at least three individuals (two females and one male) inside SVU, thus confirming at least temporary presence of this top predator in this important protected area. It is now crucial to intensify studies in that area and surroundings, to evaluate the status of these individuals and identify conservation needs to urgently improve the prospects for the establishment of a resident population, allowing it to expand to adjoining units of the Serra do Mar State Park and Serra da Bocaina National Park.

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Food habits of two syntopic canids, the maned wolf (Chrysocyon brachyurus) and the crab-eating fox (Cerdocyon thous), in southeastern Brazil

Revista chilena de historia natural ◽

10.4067/s0716-078x2004000100002 ◽

2004 ◽

Vol 77 (1) ◽

Cited By ~ 3

Author(s):

ADRIANA DE ARRUDA BUENO ◽

JOSÉ CARLOS MOTTA-JUNIOR

Keyword(s):

Food Habits ◽

Southeastern Brazil ◽

Maned Wolf ◽

Cerdocyon Thous ◽

Chrysocyon Brachyurus

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Gastrointestinal parasites of maned wolf (Chrysocyon brachyurus, Illiger 1815) in a suburban area in southeastern Brazil

Brazilian Journal of Biology ◽

10.1590/1519-6984.20013 ◽

2015 ◽

Vol 75 (3) ◽

pp. 643-649 ◽

Cited By ~ 4

Author(s):

RL Massara ◽

AMO Paschoal ◽

AG Chiarello

Keyword(s):

Disease Transmission ◽

Human Impacts ◽

Gastrointestinal Parasites ◽

Domestic Dogs ◽

Southeastern Brazil ◽

Disturbed Areas ◽

Maned Wolf ◽

High Prevalence ◽

Wild Canid ◽

Chrysocyon Brachyurus

AbstractWe examined 42 maned wolf scats in an unprotected and disturbed area of Cerrado in southeastern Brazil. We identified six helminth endoparasite taxa, being Phylum Acantocephala and Family Trichuridae the most prevalent. The high prevalence of the Family Ancylostomatidae indicates a possible transmission via domestic dogs, which are abundant in the study area. Nevertheless, our results indicate that the endoparasite species found are not different from those observed in protected or least disturbed areas, suggesting a high resilience of maned wolf and their parasites to human impacts, or a common scenario of disease transmission from domestic dogs to wild canid whether in protected or unprotected areas of southeastern Brazil.

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Determination of the Minimum Population Size of Pumas (Puma concolor) Through Fecal DNA Analysis in Two Protected Cerrado Areas in the Brazilian Southeast

Biotropica ◽

10.1111/j.1744-7429.2007.00315.x ◽

2007 ◽

Vol 39 (5) ◽

pp. 647-654 ◽

Cited By ~ 34

Author(s):

Renata Alonso Miotto ◽

Fernando Pacheco Rodrigues ◽

Giordano Ciocheti ◽

Pedro Manoel Galetti

Keyword(s):

Population Size ◽

Dna Analysis ◽

Puma Concolor ◽

Fecal Dna

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Hepatozoon sp. gamonts as an accidental finding in synovial liquid from an injured maned wolf (Chrysocyon brachyurus) in southeastern Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612019062 ◽

2019 ◽

Vol 28 (4) ◽

pp. 779-785 ◽

Cited By ~ 2

Author(s):

Lívia Perles ◽

Guilherme Henrique Fernandes Barranco ◽

Isabela Maciel Soriano ◽

Nathan da Rocha Neves Cruz ◽

Patrícia Jábali Bueno ◽

...

Keyword(s):

Clinical Pathology ◽

Skin Lesions ◽

18S Rrna Gene ◽

Southeastern Brazil ◽

Hepatozoon Canis ◽

Rrna Gene ◽

Maned Wolf ◽

The Right ◽

Domestic Carnivores ◽

Chrysocyon Brachyurus

Abstract A free-living, adult male maned wolf (Chrysocyon brachyurus) was referred to the Governador “Laudo Natel” – FCAV/Unesp veterinary hospital after being found with skin lesions and a fracture on the right pelvic limb, which had to be amputated due to compromised integrity. Around 20 days later, bilateral accentuated swollen on humerus-radius-ulna articulation was observed. The synovial liquid was drained and sent to the laboratory for synovial cytology with Rosenfeld staining that revealed predominantly degenerated neutrophils with karyolytic chromatin associated with intracellular inclusions suggestive of Hepatozoon sp. gametocytes. Blood and synovial liquid samples were submitted to molecular analysis, aiming to amplify the Hepatozoon spp. 18S rRNA gene fragment. Despite the positioning of the found Hepatozoon sequence together with Hepatozoon canis previously detected in domestic carnivores, the BLAST analysis showed only 98% identity with H. canis. To the best of the authors’ knowledge, this is the first time a Hepatozoon was detected in the synovial liquid by clinical pathology and molecular analyses.

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Genetic Characteristics of Brown Trout Salmo trutta from the Tatra National Park in Poland

Turkish Journal of Fisheries and Aquatic Sciences ◽

10.4194/1303-2712-v21_10_01 ◽

2021 ◽

Vol 21 (10) ◽

pp. 479-490

Author(s):

Marcin Kuciński ◽

Jacek Kozłowski ◽

Krzysztof Kozłowski ◽

Piotr Dynowski ◽

Dorota Fopp-Bayat ◽

...

Keyword(s):

Genetic Diversity ◽

Population Size ◽

Brown Trout ◽

National Park ◽

Dna Analysis ◽

Haplotype Diversity ◽

Global Test ◽

Genetic Characteristics ◽

Genetic Diversity And Structure ◽

Tatra National Park

In the present study the genetic diversity and structure of the brown trout in the Tatra National Park (TNP) was analyzed by application of ten microsatellites and one mitochondrial DNA markers. For this purpose, a total of 96 fish from 7 main streams located on the territory of the TNP were sampled. Examined fish was characterized by a moderate level of the genetic diversity (I=1.196-1.549) and PIC=0.567-0.707). The mean values of observed (Ho) and expected heterozygosity (He) varied from 0,577 to 0,760 and from 0,609 to 0,723, respectively. Based on genetic analysis, five haplotypes were identified, and the computed haplotype diversity (h) and nucleotide diversity (π) values were 0.561 and 0.001, respectively. Assessed genetic differentiation between the examined groups varied from 0.016 do 0.085 and the global test of differentiation (Fst=0.062) among groups provided statistically insignificant values. Constructed Bayesian skyline plot revealed stable population size, however an evidence of a historical decline of the population size in the studied fish was detected. Similarly, to microsatellite DNA analysis, the obtained data on mtDNA analysis showed weak genetic clustering, where two genetic clusters were identified, however, haplotype frequencies did not follow geographical pattern.

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Feeding ecology of the maned wolf, Chrysocyon brachyurus (Illiger, 1815) (Mammalia: Canidae), in the Ecological Station of Itirapina, São Paulo state, Brazil

Biota Neotropica ◽

10.1590/s1676-06032002000200007 ◽

2002 ◽

Vol 2 (2) ◽

pp. 1-9 ◽

Cited By ~ 16

Author(s):

Adriana de Arruda Bueno ◽

Sonia Cristina da Silva Belentani ◽

José Carlos Motta-Junior

Keyword(s):

Small Mammals ◽

Feeding Ecology ◽

Sao Paulo ◽

Southeastern Brazil ◽

São Paulo ◽

Paulo State ◽

Maned Wolf ◽

Management Plans ◽

São Paulo State ◽

Chrysocyon Brachyurus

The feeding ecology of the maned wolf (Chrysocyon brachyurus) was studied from 1998 to 2002 in the Ecological Station of Itirapina, São Paulo State, southeastern Brazil, including estimates of prey number and biomass consumption. A total of 325 faecal samples was collected in the area. The species is omnivorous, with a broad diet including 68 species or morphospecies of fruits and animals. Armadillos (Dasypodidae), wolf's fruit (Solanum lycocarpum) and small mammals (mostly Clyomys bishopi) were the bulk of the diet, comprising 72.8 % of the total estimated biomass consumed (185,323.4 g). In terms of frequency of occurrence, on the other hand, only small mammals and other miscella-neous fruits yielded 43.4 % of the total occurrences (N = 1,054). Animal prey ranging from 0.01 and 0.1 Kg were the most captured category, resulting in 44.2 % of 507 captured animals. The maned wolf seems to be seasonally opportunistic, at least for fruits and insects, as indicated by the variation of the consumption of these items along the year. The higher consumption of fruits and animals characteristic of savannah can be an important factor to consider in future management plans for the species.

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