scholarly journals Hepatozoon sp. gamonts as an accidental finding in synovial liquid from an injured maned wolf (Chrysocyon brachyurus) in southeastern Brazil

2019 ◽  
Vol 28 (4) ◽  
pp. 779-785 ◽  
Author(s):  
Lívia Perles ◽  
Guilherme Henrique Fernandes Barranco ◽  
Isabela Maciel Soriano ◽  
Nathan da Rocha Neves Cruz ◽  
Patrícia Jábali Bueno ◽  
...  
Keyword(s):  
Clinical Pathology ◽  
Skin Lesions ◽  
18S Rrna Gene ◽  
Southeastern Brazil ◽  
Hepatozoon Canis ◽  
Rrna Gene ◽  
Maned Wolf ◽  
The Right ◽  
Domestic Carnivores ◽  
Chrysocyon Brachyurus

Abstract A free-living, adult male maned wolf (Chrysocyon brachyurus) was referred to the Governador “Laudo Natel” – FCAV/Unesp veterinary hospital after being found with skin lesions and a fracture on the right pelvic limb, which had to be amputated due to compromised integrity. Around 20 days later, bilateral accentuated swollen on humerus-radius-ulna articulation was observed. The synovial liquid was drained and sent to the laboratory for synovial cytology with Rosenfeld staining that revealed predominantly degenerated neutrophils with karyolytic chromatin associated with intracellular inclusions suggestive of Hepatozoon sp. gametocytes. Blood and synovial liquid samples were submitted to molecular analysis, aiming to amplify the Hepatozoon spp. 18S rRNA gene fragment. Despite the positioning of the found Hepatozoon sequence together with Hepatozoon canis previously detected in domestic carnivores, the BLAST analysis showed only 98% identity with H. canis. To the best of the authors’ knowledge, this is the first time a Hepatozoon was detected in the synovial liquid by clinical pathology and molecular analyses.

scholarly journals Crassicauda boopis in a fin whale (Balaenoptera physalus) ship-struck in the eastern North Atlantic Ocean

2017 ◽  
Vol 3 ◽  
Author(s):  
LAETITIA LEMPEREUR ◽  
MORGAN DELOBELLE ◽  
MARJAN DOOM ◽  
JAN HAELTERS ◽  
ETIENNE LEVY ◽  
...  
Keyword(s):  
North Atlantic ◽  
18S Rrna ◽  
North Atlantic Ocean ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
Balaenoptera Physalus ◽  
Fin Whale ◽  
Eastern North Atlantic ◽  
The Right

SUMMARY On 9 November 2015, a juvenile male fin whale of 11·60 m length was observed on the bulb of a merchant vessel in the Channel Terneuzen – Ghent (The Netherlands – Belgium). A severe parasitosis was present in the right heart ventricle and caudal caval vein. Parasites were identified as Crassicauda boopis based on macroscopic and microscopic observations. The sequence of the 18S rRNA gene obtained from the parasite samples was 100% similar to the sequence of the 18S rRNA gene from Crassicauda magna available on GenBank. While adults of C. boopis and C. magna are morphologically distinct and found at different locations in the body, the molecular analysis of the 18S rRNA gene seems insufficient for reliable species identification. Although numerous C. boopis were found, the cause of death was identified as due to the collision with the ship, as suggested by the presence of a large haematoma, and the absence of evidence of renal failure. The young age of this whale and the absence of severe chronic reaction may suggest that the infestation was not yet at an advanced chronic stage.

Primary cutaneous nocardiosis of the head and neck in an immunocompetent patient

2021 ◽  
Vol 14 (5) ◽  
pp. e241217
Author(s):  
Claudio Tirso Acevedo ◽  
Frank Imkamp ◽  
Ewerton Marques Maggio ◽  
Silvio Daniel Brugger
Keyword(s):  
Head And Neck ◽  
Skin Lesions ◽  
Immunocompetent Patient ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Rare Manifestation ◽  
Life Threatening ◽  
Amoxicillin Clavulanic Acid ◽  
Disseminated Nocardiosis ◽  
The Right

Nocardiosis is known to be an opportunistic infection most commonly affecting immunocompromised patients that can lead to life-threatening conditions. Primary cutaneous disease remains a rare manifestation and unlike pulmonary or disseminated nocardiosis, it usually affects immunocompetent individuals. We present a case of a primary cutaneous nocardiosis of the head and neck after an insect bite in a healthy 50-year-old woman who had recently travelled from Greece. She presented with a painful right-sided swelling of her face and neck and an ulcerated plaque over the right temple. Biopsy of the plaque revealed inflammation with abscess formation indicating underlying infection. Culture from the biopsy showed growth of Nocardia spp and 16S rRNA gene sequence analysis identified Nocardia brasiliensis. The patient was treated with trimethoprim/sulfamethoxazole and subsequently switched to amoxicillin/clavulanic acid due to a drug eruption. Antibiotic therapy was continued for a total of 3 months with complete resolution of the skin lesions.

scholarly journals Hepatozoon canis and Leishmania spp. coinfection in dogs diagnosed with visceral leishmaniasis

2016 ◽  
Vol 25 (4) ◽  
pp. 450-458 ◽  
Author(s):  
Fernanda Nazaré Morgado ◽  
Amanda dos Santos Cavalcanti ◽  
Luisa Helena de Miranda ◽  
Lúcia Helena O’Dwyer ◽  
Maria Regina Lucas da Silva ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Dna Isolation ◽  
Parasite Burden ◽  
Leishmania Species ◽  
18S Rrna Gene ◽  
Hepatozoon Canis ◽  
Histopathological Analysis ◽  
Rrna Gene ◽  
Pcr Rflp ◽  
Leishmania Spp

Abstract This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1' PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP - Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression.

scholarly journals Gastrointestinal parasites of maned wolf (Chrysocyon brachyurus, Illiger 1815) in a suburban area in southeastern Brazil

2015 ◽  
Vol 75 (3) ◽  
pp. 643-649 ◽  
Author(s):  
RL Massara ◽  
AMO Paschoal ◽  
AG Chiarello
Keyword(s):  
Disease Transmission ◽  
Human Impacts ◽  
Gastrointestinal Parasites ◽  
Domestic Dogs ◽  
Southeastern Brazil ◽  
Disturbed Areas ◽  
Maned Wolf ◽  
High Prevalence ◽  
Wild Canid ◽  
Chrysocyon Brachyurus

AbstractWe examined 42 maned wolf scats in an unprotected and disturbed area of Cerrado in southeastern Brazil. We identified six helminth endoparasite taxa, being Phylum Acantocephala and Family Trichuridae the most prevalent. The high prevalence of the Family Ancylostomatidae indicates a possible transmission via domestic dogs, which are abundant in the study area. Nevertheless, our results indicate that the endoparasite species found are not different from those observed in protected or least disturbed areas, suggesting a high resilience of maned wolf and their parasites to human impacts, or a common scenario of disease transmission from domestic dogs to wild canid whether in protected or unprotected areas of southeastern Brazil.

scholarly journals Genetic and Antigenic Evidence Supports the Separation of Hepatozoon canis and Hepatozoon americanum at the Species Level

2000 ◽  
Vol 38 (3) ◽  
pp. 1298-1301 ◽  
Author(s):  
Gad Baneth ◽  
John R. Barta ◽  
Varda Shkap ◽  
Donald S. Martin ◽  
Douglass K. Macintire ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
18S Rrna ◽  
Distinct Species ◽  
18S Rrna Gene ◽  
Species Level ◽  
Hepatozoon Canis ◽  
Rrna Gene ◽  
Western Immunoblotting ◽  
Hepatozoon Americanum

Recognition of Hepatozoon canis and Hepatozoon americanum as distinct species was supported by the results of Western immunoblotting of canine anti-H. canis and anti-H. americanum sera against H. canisgamonts. Sequence analysis of 368 bases near the 3′ end of the 18S rRNA gene from each species revealed a pairwise difference of 13.59%.

scholarly journals Malignant pancreatic gastrinoma in a maned wolf (Chrysocyon brachyurus): case report

2018 ◽  
Vol 70 (2) ◽  
pp. 475-478
Author(s):  
T.F. Carvalho ◽  
D.N. Frois ◽  
H.P. Tinoco ◽  
A.T. Pessanha ◽  
T.A. Paixão ◽  
...  
Keyword(s):  
Case Report ◽  
Lymph Node ◽  
Visceral Leishmaniasis ◽  
Pancreatic Polypeptide ◽  
Neoplastic Cells ◽  
Solid Nodule ◽  
Maned Wolf ◽  
The Right ◽  
Chrysocyon Brachyurus ◽  
Pancreatic Lymph Node

ABSTRACT A captive 12-year-old female maned wolf (Chrysocyon brachyurus), previously diagnosed with visceral leishmaniasis, developed severe myiasis in the right ear, followed by prostration and death. A firm solid nodule, measuring 1 x 3cm, was grossly observed in the pancreas. Histologically, there was neoplastic proliferation in the pancreas and pancreatic lymph node. Neoplastic cells had a neuroendocrine pattern, and were positive for gastrin and pancreatic polypeptide by immunohistochemistry. The wolf had also ulcerative gastritis. To the best of our knowledge is the first reported case gastrinoma in this species.

scholarly journals Sarcocystis gigantea infection associated with granulomatous eosinophilic myositis in a horse

2020 ◽  
Vol 32 (4) ◽  
pp. 611-615
Author(s):  
Fabrizia Veronesi ◽  
Stefano Di Palma ◽  
Simona Gabrielli ◽  
Giulia Morganti ◽  
Giovanni L. Milardi ◽  
...  
Keyword(s):  
Incidental Finding ◽  
Pectoral Muscle ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Sarcocystis Species ◽  
Sarcocystis Spp ◽  
Formalin Fixed Paraffin Embedded ◽  
Veterinary Surgeon ◽  
Specific Species ◽  
The Right

The only Sarcocystis species currently known to inhabit the fibers of skeletal and cardiac muscles in horses are S. fayeri, S. bertrami, and S. asinus. We describe herein the invasion of myofibers in a horse by S. gigantea, a sheep-specific species with low virulence in the original host. A hunter gelding was referred to a veterinary surgeon in Newmarket (UK). The anamnestic data reported that the horse had an initial history of swelling of the right forelimb with fluid on the front of the carpus and edema spreading up the forearm. Subsequently, 2 firm lumps were found on the left pectoral muscle adjacent to the axilla of the left forelimb. Histologic examination of biopsies from the lumps revealed multifocal granulomatous eosinophilic myositis associated with intact and degenerate encysted parasites, consistent with Sarcocystis spp. Based on amplification and DNA sequencing of the 18S rRNA gene obtained from formalin-fixed, paraffin-embedded tissue blocks, S. gigantea was identified. The presence of sarcocysts in equine skeletal muscles has been considered an incidental finding, and there are only sporadic associated reports of myositis. Our finding suggests that some Sarcocystis spp. have a wider intermediate host range than believed previously, and that Sarcocystis of other species (not considered horse-associated) can invade the muscle fibers of equids, leading to myositis.

scholarly journals Extraction of Unerupted Maxillary Canine Teeth in a Maned Wolf (Chrysocyon brachyurus)

2016 ◽  
Vol 2016 ◽  
pp. 1-3
Author(s):  
Lourdes M. B. Pessoa ◽  
Marcello Roza ◽  
Anderson Farias ◽  
Pedro Henrique de Jesus ◽  
Rita de Cassia Campbell ◽  
...  
Keyword(s):  
Complete Blood Count ◽  
Canine Tooth ◽  
Maxillary Canine ◽  
Maned Wolf ◽  
Serum Biochemical ◽  
Serum Biochemical Profile ◽  
Mandibular Canine ◽  
The Right ◽  
Chrysocyon Brachyurus

The purpose of this case report is to describe the diagnosis and treatment of unerupted canine teeth in a maned wolf. After physical examination, complete blood count, and serum biochemical profile, the animal underwent general anesthesia and head radiography was performed to confirm the diagnosis. The treatment consisted of the extraction of both maxillary canine teeth and clinical and radiographic follow-up of the right mandibular canine tooth.

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