Vertical Transmission of PCV2b to Fetuses in Sows Intramuscularly Infected with PCV2b

Abstract In order to investigate whether PCV2b can be transmitted across the placenta in sows thereby causing vertical infection of fetuses, six sows in 75 day of pregnancy were either intramuscularly inoculated with a PCV2b culture supernatant (n = 4) or mock infected with cell culture supernatant (n = 2). At parturition, 3 newborn piglets from each litter were randomly selected and euthanatized prior to suckling. Samples of the mesenteric lymph nodes, spleens, kidneys, hearts and lungs were collected for PCR, histopathological and immunohistochemical assays. The results showed that the newborn piglets from PCV2b-infected sows had histopathological lesions in the tested tissues. Moreover, PCV2b antigen and DNA were detected as well. These findings therefore suggested that porcine circovirus type 2b can be transmitted across the placenta of sows, thereby leading to PCV2b vertical infection of the fetuses.

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Germocyte lesions caused by porcine circovirus type 2b in Kunming mice

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2017-0004 ◽

2017 ◽

Vol 20 (1) ◽

pp. 25-29 ◽

Cited By ~ 1

Author(s):

A.W. Yuan ◽

P. Pang ◽

Q.L. Gong ◽

Z.B. Deng

Keyword(s):

Cell Culture ◽

Mouse Model ◽

Post Infection ◽

Leydig Cells ◽

Culture Supernatant ◽

Porcine Circovirus Type ◽

Porcine Circovirus ◽

Cell Culture Supernatant ◽

Female Mice

Abstract Porcine circovirus type 2 (PCV2) has been known as a causative agent of reproductive failure in the sow. In the present study mouse model was used to investigate PCV2 infection. In order to investigate whether PCV2 can induce lesions of spermatocytes and oocytes, 6 male and 6 female mice were each inoculated intraperitoneally with PCV2b, and 3 male and 3 female mice mock-infected with cell culture supernatant served as controls. Samples of testes and ovaries from PCV2b-inoculated and mock-infected mice were investigated using PCR, histopathological, ultrastructural and immunofluorescent histochemical methods at 14 and 21 day post infection (dpi). The study revealed that in the virus-inoculated mice leydig cells in testes and granulosa cells in ovaries were degenerated, and a small number of spermatocytes and oocytes showed apoptosis. Positive PCV2b antigen signals were also observed in these apoptotic cells. It can be suggested that PCV2 can cause lesions of spermatocyte and oocyte prior to zygotes formation in its host.

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Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2008000600009 ◽

2008 ◽

Vol 28 (6) ◽

pp. 313-318 ◽

Cited By ~ 7

Author(s):

Priscila Zlotowski ◽

André M.R. Corrêa ◽

David E.S.N. Barcellos ◽

Cláudio E.F. Cruz ◽

William Asanome ◽

...

Keyword(s):

Lymph Nodes ◽

Villous Atrophy ◽

Clinical Signs ◽

Postweaning Multisystemic Wasting Syndrome ◽

Porcine Circovirus ◽

Mesenteric Lymph Nodes ◽

Salmonella Spp ◽

Wasting Syndrome ◽

Mesenteric Lymph ◽

Intestinal Lesions

Samples of mesenteric lymph nodes and intestines from 79 unthrifty 3- to 5-month-old postweaning pigs, confirmed as naturally affected with postweaning multisystemic wasting syndrome (PMWS), were studied. Pigs originated from 12 farms in southern Brazil and were selected on the basis of clinical signs and/or gross lesions suggestive of enteric disorder. Lymphohistiocytic infiltrates of varying intensity were associated with anti-porcine circovirus type 2 (anti-PCV2) immunostaining (IS) in samples of intestines and mesenteric lymph nodes from all pigs. Although most findings were similar to those described in PCV2-associated enteritis, anti-PCV2 IS in association with depletion of the goblet cell mucin stores (24 pigs), diffuse ileal villous atrophy and fusion (18 pigs), and dilatation of the lymphatic vessels (11 pigs) combined or not with lymphangitis were also observed. PCV2 antigen was immunohistochemically demonstrated in the cytoplasm and nuclei from intralesional epithelial cells, histiocytes, and endothelial-like cells in intestinal tissues. Together these findings imply an association with PCV2. The presence of co-infections by Lawsonia intracellularis, Brachyspira spp., Mycobacterium spp., Salmonella spp., rotavirus, parvovirus, coronavirus and enteric calicivirus with PCV2 in the intestinal lesions was investigated.

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Diagnostic Characteristics of Circovirus Infection in Pigs

Macedonian Veterinary Review ◽

10.14432/j.macvetrev.2014.11.033 ◽

2015 ◽

Vol 38 (1) ◽

pp. 73-78

Author(s):

Ivica Gjurovski ◽

Branko Angelovski ◽

Toni Dovenski ◽

Dine Mitrov ◽

Trpe Ristoski

Keyword(s):

Lymph Nodes ◽

Porcine Circovirus Type ◽

Diagnostic Methods ◽

Immunohistochemical Method ◽

Porcine Circovirus ◽

Mesenteric Lymph Nodes ◽

Wasting Syndrome ◽

Diagnostic Characteristics ◽

Nonenveloped Virus ◽

Almost All

Abstract The aim of this study is to compare the results from the histopathology and the immunohistochemical method in the diagnostic of Porcine circovirus type 2 (PCV2) infection in pigs. The circovirus infection is a pig disease that is caused by a small, spherical, nonenveloped virus with a single stranded DNA genome which is spread throughout the pig industry worldwide. The circovirus is the etiological agent of a several pig diseases which today are thought to be the cause of the greatest economical loses in pig production. The most important of these diseases is the PMWS (post-weaning multisystemic wasting syndrome). In this article we have performed an investigation of four farms on which there had been a previous clinical diagnosis of the Post-weaning multisystemic wasting syndrome. The examination was performed on thirty pigs from these farms, from two to five months old, which had the most severe symptoms of the disease. Necropsy, histopathology and immunohistochemical diagnostic methods were performed. The most significant necropsy findings were the enlarged lymph nodes (especially the inguinal, mediasinal and the mesenteric lymph nodes). The main histopathological changes were located in the lymphatic organs presented by B and T lymphocyte depletion and increase in the number of the macrophages. PCV2 antigen and nucleic acid were detected in almost all of the examined tissues. The examination showed that the histopathological and immunohistochemical methods provide complementary results in diagnosing PCV2 in pigs.

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