scholarly journals Effect of ethanol and plant growth regulators on termination of potato microtuber dormancy

2015 ◽  
Vol 71 (1) ◽  
pp. 23-36
Author(s):  
Sławomir Wróbel ◽  
Jacek Kęsy ◽  
Krzysztof Treder
Keyword(s):  
Aqueous Solution ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Standard Treatment ◽  
Control Treatment ◽  
Ethanol Treatment ◽  
Dormancy Breaking ◽  
Natural Decrease ◽  
Microtuber Production

Abstract The duration of dormancy varies significantly among cultivars, but even short dormancy can limit usage of potato microtubers for seed production. The aim of the research was to test efficacy of dormancy breaking by treatment with an aqueous solution of ethyl alcohol, saccharose, gibberellic acid and kinetin (ethanol treatment) in comparison to treatment with aqueous solution of thiourea, daminozide and gibberellic acid (standard treatment). Prolonging the period of microtuber production at the in vitro stage significantly favored the short-ening of the dormancy and facilitated its breaking. While the standard treatment had the strongest effect, the ethanol treatment was slightly less efficient. The statistically significant differences were only observed during the first 13 days after the microtuber treatment. After that time, efficacy of ethanol and standard treatments was similar to control treatment with water. The investigated treatments had no effect on the natural decrease of ABA level in microtubers.

scholarly journals 300 Effects of Plant Growth Regulators on Eggplant (Solanum melongena L.) Yield

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 443C-443
Author(s):  
J. Pablo Morales-Payan
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Fruit Set ◽  
Block Design ◽  
Control Treatment ◽  
Amine Hydrochloride ◽  
Yield Increase ◽  
Giberellic Acid

Field studies were conducted in the Dominican Republic to determine the effect of several plant growth regulators on the yield of `Jira' eggplant. Treatments consisted of aqueous solutions of folcysteine (25, 50, 75 ppm), giberellic acid 3 (10, 20, 30 ppm), kinetine (25, 50, 75 ppm), naphthalenacetic acid (NAA) (25, 50, 75 ppm), 2,3,4-dichloro-phenoxy-triethyl-amine hydrochloride (DCPTA) (25, 50, 75 ppm), triacontanol (5, 10, 15 ppm), ethanol (5, 10, 15%), and chlormequat (50, 100, 150 ppm) sprayed at early flowering, directed to the crop upper leaves and flowers. A control treatment (no plant growth regulators applied) was also included. A randomized complete-block design with four replications was utilized. Experimental units were two rows of 10 plants at a 1.0 × 0.4-m distancing. Eggplant fruit set and yield were determined after 10 harvests performed at 3-day intervals. Analysis of variance and mean comparison tests were performed on the resulting data. `Jira' eggplant fruit set and yield was significantly improved by folcysteine, giberellic acid 3, and NAA, but not by kinetine, DCPTA, ethanol, triacontanol, or chlormequat. Eggplant yield increased as folcysteine rate increased from 0 to 50 ppm, but no further yield increase was obtained when increasing the rate from 50 to 75 ppm. Similarly, eggplant yield significantly increased as gibberellic acid increased from 0 to 20 ppm, but not when rates increased from 20 to 30 ppm. With NAA, eggplant fruit set and yield significantly increased above that of control plants when 25 ppm was applied, with no significant yield increase at higher rates. Results indicate that the yield of `Jira' eggplants could be enhanced by the treatments with either folcysteine, NAA, or gibberellic acid hereby described.

scholarly journals EFFECT OF EXPALNT TYPE AND SOME PLANT GROWTH REGULATORS ON CULTURE INITIATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Khierallah & Al-Obaidy
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Control Treatment ◽  
Ms Medium ◽  
Explant Type ◽  
Culture Initiation ◽  
The Impact

This research was conducted in order to study the effect of explant type and some plant growth regulators on culture initiation of Stevia rebaudiana Bertoni in vitro. The experiments included surface sterilization and test two types of explants (shoot tips and stem nodes) and the impact of KIN and BA and IAA and IBA in the cultures initiation. Results revealed the efficiency of sodium hypochlorite (NaOCl) for disinfestation of explant at 0.050% concentration giving less contamination for shoot tips and stem nods (10% and 20% respectively). Results showed that shoot tips inoculated in MS medium plus KIN at 0.3 mg. L-1 was significantly increase the number of regenerated shoots as it produced 4.2 shoots per explant while medium without cytokinin (control) produced less number of shoots reached 1.4 shoots per explant. KIN treatment reduced shoots length as control treatment produced the highest length (6.74 cm).  The interaction between the explant type and BA concentration was significantly increase the number of regenerated shoots as shoot tips produced 3.6 shoots per explant in MS medium supplemented with 0.1 mg. L-1. BA treatment reduced shoots length as control treatment produced the highest length (6.74 cm). No positive effect was gain when auxins (IBA and IAA) were added in combination with cytokinin in culture medium. The above results can be adopted to established stevia in vitro culture successfully.

Media optimization and the effect of benzyl amino purine (BAP) and gibberellic acid (GA3) on the shooting of Pterocarpus erinaceus Poir.

2020 ◽  
Vol 16 (1) ◽  
pp. 32-39
Author(s):  
M.E. Olorode ◽  
E.A. Adekunle
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Forest Tree ◽  
Shoot Length ◽  
Media Optimization ◽  
Mass Propagation ◽  
Experimental Conditions ◽  
Over Exploitation ◽  
Benzyl Amino Purine

Ptreocarpus erinaceus is a very useful forest tree due to its economic and medicinal values. It is also used as fodder for livestock due to its high nitrogen content. Its numerous use attract the high demand leading to it’s over exploitation. However, the tree is slow growing in nature. Hence, there is a need to look for in vitro methods for its mass propagation as an alternative method to raise healthy trees and sustain its availability. Media optimization gave two best performing media; Driver and kuniyuki Walnut (DKW) and Murashige and Skoog MS) which were further supplemented with different combinations of plant growth regulators (PGR)-Benzyl Amino Purine (BAP) and Gibberellic Acid (GA3). Overall, the DKW media with BAP/GA3 at 1.0/0.5 produced the highest shoot length while BAP/GA3 at 4.0/0.0 gave the highest number of leaves after eight weeks of inoculation. Effective utilization of these experimental conditions could be the impetus towards mass propagation of this important tree species. Keywords: Ptreocarpus erinaceus, media optimization, Plant growth regulator, shoot length, mass propagation

scholarly journals Breaking seed dormancy of Tulipa scardica Bornm. and Tulipa kosovarica Kit Tan, Shuka & Krasniqi by pre-chilling, plant growth regulators and some chemical treatments

2019 ◽  
Vol 113 (2) ◽  
pp. 203 ◽  
Author(s):  
Bekim GASHI ◽  
Mirsade OSMANI ◽  
Sali ALIU
Keyword(s):  
Sulfuric Acid ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Dormancy Breaking ◽  
Germination Time ◽  
Mean Germination Time

<p><em>Tulipa scardica</em> and <em>T. kosovarica</em> are rare, endemic and endangered plants in Kosovo. This research was carried out to study the dormancy breaking treatment in Tulipa scardica and T. kosovarica seeds by pre-chilling and various concentration of potassium nitrate (KNO3), gibberellic acid (GA3), kinetin, thiourea and sulfuric acid (H2SO4). The experiment was conducted with untreated seeds (without stratification) and with stratified seeds (8 weeks) and treated with different concentration of some chemicals and plant growth regulators. Results showed that the final germination percentage (FGP) of seeds without stratification at both Tulipa plant species was zero, while germination was enhanced by pre-chilling (stratification) especially after treatment of these seeds by above mentioned treatments. Both Tulipa species showed an increase in mean germination time (MGT), an indication of slower germination, as different chemicals or plant growth regulators increased. Depending on treatments, germination was ranging from 80 % to 90 %, the maximum germination was detected in seeds treated with KNO3, GA3, and their combination, while the minimum germination in seeds without treatments and treated with thiourea. The conservation of these plants in botanic gardens by cultivation or propagation for commercial use as ornamental plants could give an effective contribution to the conservation of these plants.<br />Abbreviations: KNO<sub>3 </sub><strong>– </strong>potassium  nitrate; GA<sub>3 </sub>- gibberellic acid; H<sub>2</sub>SO<sub>4</sub> – sulfuric acid; FGP – final germination percentage; MGT – mean germination time<br /><br /></p>

scholarly journals The effect of different treatments on seed dormancy breaking and germination inducing in Louisiana variety of Okra (Abelmoschus esculentus L.)

2020 ◽  
Vol 3 (4) ◽  
pp. 124-128
Author(s):  
Ali Jawed Safdary ◽  
Ahmad Jawid Ahamdi ◽  
Nasratullah Habibi ◽  
Zabiullah Rahmani ◽  
Saifullah Rasooli
Keyword(s):  
Hydrochloric Acid ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Cold Treatment ◽  
Winter Season ◽  
Dry Weight ◽  
Hot Water ◽  
Control Treatment ◽  
Dormancy Breaking

To investigate the methods of dormancy breaking in okra seeds; a study was conducted in the winter season of 2019 under laboratory conditions in Samangan Higher Education Institute. The experiment was carried out based on a completely randomized design within 10 treatments and 3 replications. The treatments included gibberellic acid at 4 levels (100, 500, 700, and 1000 ppm), hot water (70°C), hydrochloric acid (75%) at three levels (5, 10, and 15 minutes), 7-day chilling at 3°C and control treatment (distilled water). The results showed that the highest percentage (100%) with a germination rate of 86 seeds were available due to the application of 7-day cold treatment. Moreover, the treatments such as 7-day chilling, gibberellic acid 500 ppm, and hydrochloric acid 10 and 15 minutes had significant effects on the fresh and dry weight of roots and shoots as well as on their length. we observed that the 7- day cooling at 3°C is the best and most appropriate solution to break down the seed dormancy and stimulate seed germination of the above-mentioned crop.

scholarly journals In vitro propagation from nodal segments of Lippia origanoides (chemotype A)

2022 ◽  
Vol 52 (7) ◽  
Author(s):  
Julián Sebastián Ramírez Moreno ◽  
Sergio Andrés Vega Porras ◽  
Roosevelt Humberto Escobar ◽  
Elena E. Stashenko ◽  
Jorge Luis Fuentes Lorenzo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Nodal Segments ◽  
Ms Medium ◽  
Shoot Number ◽  
Murashige And Skoog Medium

ABSTRACT: This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia.

Efficiency of indoleacetic acid, gibberellic acid and ethylene synthesized in vitro by Fusarium culmorum strains with different effects on cereal growth

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Jolanta Jaroszuk-Ściseł ◽  
Ewa Kurek ◽  
Mariusz Trytek
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Indoleacetic Acid ◽  
Fusarium Culmorum ◽  
High Capacity ◽  
Growth Conditions ◽  
Optimal Conditions ◽  
High Positive Correlation ◽  
Growth Promoting

AbstractThree different Fusarium culmorum strains having a pathogenic, a deleterious (deleterious rhizosphere microorganism), or a promoting (plant growth promoting fungus) effect on plant growth were studied for their ability to synthesize in vitro the phytohormones indoleacetic acid (IAA), gibberellic acid (GA), and ethylene. All the phytohormones tested were synthesized in cultures supplemented with wide concentration ranges of glucose and tryptophan or methionine (precursors of phytohormone synthesis). The amounts of these secondary metabolites synthesized by the particular strains were found to be significantly different. The non-pathogenic PGPF strain (DEMFc2) synthesized the highest amounts of IAA and GA, a fact that could be responsible for the growth-promoting properties of this strain. A pathogenic strain synthesized the highest amount of ethylene, which could be responsible for the negative effect of this strain on plant growth. F. culmorum isolates with a high capacity for IAA synthesis also have a high capacity for GA synthesis and irrespective of the growth conditions, a high positive correlation (R > 0.9) between the concentrations of synthesized IAA and GA in F. culmorum cultures was found. It is worth mentioning that the optimal conditions for the growth of F. culmorum isolates and the synthesis of the individual phytohormones differed from one another. The optimal growth conditions were 1.0% of glucose and 9.9 mM of methionine or 6.0 mM of tryptophan. The optimal conditions for ethylene synthesis were 0.5% of glucose and 6.6 mM of methionine, whereas 1.0% of glucose and 9.0 mM of tryptophan were optimal for IAA and GA synthesis.

scholarly journals Response of Clementine Mandarin seeds for germination and callus initiation and Alkaloids, hormones production in vitro

2013 ◽  
Vol 7 (2) ◽  
Author(s):  
Ayad Assi Obaid ◽  
Wessam Malek Dawood ◽  
Nour Sabry Nasser
Keyword(s):  
Magnetic Field ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Control Treatment ◽  
South Pole ◽  
Callus Initiation ◽  
The Impact ◽  
North And South

This study was conducted in plant tissue culture Lab. Horticulture Dept., College of Agriculture, Diyala University to evaluated the effect of plant growth regulators, North and South pole of magnetic field on common mandarin (Citrus reticulata L. Blanco) seedling growth and callus initiation. Seeds were cultured on solidified MS medium to study the impact of increasing magnetic field flood on the production of some plant growth regulators, alkaloids callus initiation. Results can be summarized as follows: Kinetin at concentrations (0.0, 1.0, 2.0, 3.0, 4.0) mg/L for 8 weeks of treatment showed that the control treatment gave taller roots (30) mm while the treatment 4.0 mg/L gave the highest number of shoots 1.7 shoot/ seedling. In the magnetic field experiment, the effect of north and south pole of magnetic field 200 mT for six periods (0 , 2 , 6, 11 , `14 , 18) days, showed that the treatment of 18 days gave the best shoot length 22mm. When the medium supplemented with 2mg/ l 2,4-D enhances callus initiation, while the combine of 2,4-D and TDZ reduced the rate of initiation and growth of callus. The production of hormones and alkaloids from mandarin callus exposed to1, 2 or3 magnets recorded the highest amount of Kin in the presence of 3 magnets giving 125.45 µg/ g while the highest amount of ABA, GA , IAA was found in control callus which gave (91.61, 76.25, 51.18)µg/ g respectively. Alkaloids yield showed the presence of Octapamine in the treatment of 3 magnets yielding 73.74 µg/g. The highest amount of Synephrine reached 366.99 µg/ g in the treatment of 3 magnets while the amount of Tyramine recorded 79.02 µg/ g in the treatment of two magnets. Ephedrine was higher in the callus that not being exposed reached 219.99 µg/ g.

scholarly journals Effect of gibberellic acid and the biostimulant stimulate® on the initial growth of tamarind

2012 ◽  
Vol 34 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Ana Cristina Vello Loyola Dantas ◽  
João Mariano de Oliveira Queiroz ◽  
Elvis Lima Vieira ◽  
Vanessa de Oliveira Almeida
Keyword(s):  
Aqueous Solution ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Height ◽  
Root Length ◽  
Block Design ◽  
Dry Mass ◽  
Stem Diameter ◽  
Initial Growth ◽  
Randomized Block Design

Plant growth regulators and biostimulants have been used as an agronomic technique to optimize the production of seedlings in various crops. This study aimed to evaluate the influence of gibberellic acid and the biostimulant Stimulate® on the initial growth of tamarind (Tamarindus indica L.). The experiments were conducted in a nursery with 50% shading, in a randomized block design with five replications and five plants per plot. Thirty eight days after sowing, the leaves were sprayed seven times a day with 0.0 (control), 0.8, 1.6, 2.4 and 3.2 mL of gibberellic acid L-1 aqueous solution and with 0.0 (control), 6.0,12.0, 18.0, and 24.0 mL Stimulate® L-1 aqueous solution. Stem diameter (SD), plant height (PH), longest root length (LRL), shoot dry mass (SDM), root dry mass (RDM) and RDM:SDM ratio were evaluated ninety days after sowing. Variance and regression analysis showed that GA3 at 4% promoted plant growth (height), but had no significant effect on stem diameter, longest root length, shoot and root dry mass and the RDM:SDM ratio. On the other hand, all concentrations of Stimulate® significantly increased plant height and shoot and root dry mass of tamarind seedlings.

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