In-vitro effect of antimicrobial photodynamic therapy with methylene blue in two different genera of dermatophyte fungi

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Teresita Spezzia-Mazzocco ◽  
Susana A. Torres-Hurtado ◽  
Julio Cesar Ramírez-San-Juan ◽  
Rubén Ramos-García
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Vitro Effect

In vitro effect of curcumin-mediated antimicrobial photodynamic therapy on fibroblasts: viability and cell signaling for apoptosis

2020 ◽  
Author(s):  
Giuliana Campos Chaves Lamarque ◽  
Daniela Alejandra Cusicanqui Méndez ◽  
Adriana Arruda Matos ◽  
Thiago José Dionísio ◽  
Maria Aparecida Andrade Moreira Machado ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Signaling ◽  
Antimicrobial Photodynamic Therapy ◽  
Vitro Effect

scholarly journals Antimicrobial Photodynamic Therapy in the Control of Pseudomonas syringae pv. actinidiae Transmission by Kiwifruit Pollen

2020 ◽  
Vol 8 (7) ◽  
pp. 1022
Author(s):  
Margarida M. Lopes ◽  
Maria Bartolomeu ◽  
Ana T. P. C. Gomes ◽  
Etelvina Figueira ◽  
Ricardo Pinto ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Pseudomonas Syringae ◽  
Pollen Germination ◽  
Ex Vivo ◽  
Irradiation Time ◽  
Antimicrobial Photodynamic Therapy ◽  
Bacterial Reduction ◽  
Promising Alternative

Pseudomonas syringae pv. actinidiae (Psa) is a phytopathogen responsible for bacterial canker in kiwifruit plants and can be disseminated through pollen. This study aimed to evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) in the inactivation of Psa on kiwifruit pollen using New Methylene Blue (NMB) and Methylene Blue (MB) in the presence/absence of potassium iodide (KI). Pollen germination assays were also performed to evaluate if it was affected by aPDT. Higher reduction of Psa was achieved using NMB (5.0 μM) combined with KI (100 mM) in vitro (ca. 8 log CFU mL−1 after 90 min of irradiation), while NMB alone promoted a lower reduction (3.7 log CFU mL−1). The most efficient NMB concentration with KI was used to study the photodynamic efficiency of MB (5.0 μM). MB with KI photo-inactivated Psa more efficiently than NMB, causing the same bacterial reduction (ca. 8 log CFU mL−1) in half the irradiation time (45 min). Therefore, MB was selected for the subsequent ex vivo aPDT assays in pollen. Almost all the Psa cells added artificially to the pollen (3.2 log CFU mL−1) were photo-inactivated (3.1 log CFU mL−1), whereas aPDT had a low effect on pollen natural microorganisms. When KI was added, a significant increase in aPDT effectiveness was observed (4.5 log CFU mL−1). No negative effects were observed in the pollen germination after aPDT. The results show aPDT is an effective and safe method to Psa inactivation on kiwifruit pollen, and MB use is a promising alternative in the control of Psa transmission.

scholarly journals Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

2021 ◽  
Vol 22 (20) ◽  
pp. 10971
Author(s):  
Rafał Wiench ◽  
Joanna Nowicka ◽  
Magdalena Pajączkowska ◽  
Piotr Kuropka ◽  
Dariusz Skaba ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Toluidine Blue ◽  
Incubation Time ◽  
In Vitro Study ◽  
Yeast Cells ◽  
Antimicrobial Photodynamic Therapy ◽  
Microscopic Evaluation ◽  
Huge Impact ◽  
Vitro Effect

(1) Background and the aim: The appropriate incubation time in the antimicrobial photodynamic therapy protocol seems to have a huge impact on the efficacy of this process. This is particularly important in relation to Candida strains, due to the size of these cells and the presence of the cell wall. The aims of this study were to determine the optimal incubation time needed for the absorption of toluidine blue by cells of C. albicans, C. glabrata, C. krusei and C. parapsilosis using direct observation by optical microscopy, and to evaluate the efficacy of TBO-mediated aPDT on planktonic cells of these strains. (2) Methods: The microscopic evaluation consisted of taking a series of images at a magnification of 600× and counting the % of stained cells. The in vitro effect of TBO-mediated aPDT combined with a diode laser (635 nm, 400mW, 12 J/cm2, CW) on the viability of yeast cells with different incubation times was evaluated. (3) Results: The presence of TBO within the cytoplasm was observed in all tested Candida strains and at all microscopic evaluation times. However, the highest percentages of cells were stained at 7 and 10 min. The highest % reduction of CFU/mL after TBO-mediated aPDT against Candida was obtained for the strain C. albicans ATCC 10,231 and it was 78.55%. (4) Conclusions: TBO-mediated aPDT against Candida was effective in reducing the number of CFU/mL at all assessed incubation times. However, the most efficient period for almost all strains was 7–10 min.

The in vitro effect of Antimicrobial Photodynamic Therapy on dental microcosm biofilms from partially erupted permanent molars: A pilot study

2018 ◽  
Vol 21 ◽  
pp. 163-167 ◽  
Author(s):  
Fabiana Sodré de Oliveira ◽  
Thiago Cruvinel ◽  
Daniela Alejandra Cusicanqui Méndez ◽  
Evandro José Dionísio ◽  
Daniela Rios ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Pilot Study ◽  
Antimicrobial Photodynamic Therapy ◽  
Permanent Molars ◽  
Vitro Effect

scholarly journals Effective Photodynamic Inactivation of 26 Escherichia coli Strains with Different Antibiotic Susceptibility Profiles: A Planktonic and Biofilm Study

Antibiotics ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 98 ◽  
Author(s):  
Òscar Gulías ◽  
Giselle McKenzie ◽  
Miquel Bayó ◽  
Montserrat Agut ◽  
Santi Nonell
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Reference Strain ◽  
Red Light ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Antimicrobial Photodynamic Therapy ◽  
Strain Atcc

The emergence of multidrug-resistant bacteria is a growing problem and alternative therapies are being sought to effectively address this issue. The aim of this study is to assess a range of Escherichia coli strains’ susceptibility to Methylene Blue-mediated antimicrobial photodynamic therapy and determine if this is affected by their antibiotic-resistance profile. Two reference and twenty-four uropathogenic clinical E. coli strains were used in this study. All were tested in vitro for antimicrobial susceptibility against sixteen antibiotics. Strains underwent photodynamic treatments using the photosensitizer Methylene Blue with red light and tested in both planktonic and biofilm state. It was found that reference strain ATCC 25922 was susceptible to all tested antibiotics whereas reference strain ATCC 35218 showed resistance only to Ampicillin. With the exception of strains number 16 and 22, all of the isolated strains were multidrug-resistant according to the criteria established by the European Centre for Disease Prevention and Control and the Centre for Disease Control and Prevention, where acquired non-susceptibility to at least one agent in three or more antimicrobial categories is outlined. Photodynamic therapy induced more than 3 log10 colony-forming units’ reduction to all strains in planktonic state. Whereas when tested in biofilm state, two and a half times the original dose of methylene blue was necessary to cause a 3 log10 antimicrobial effect. There were statistically significant differences in susceptibility among the strains tested in both the planktonic and biofilm experiments. Nevertheless, antimicrobial photodynamic therapy could inactivate all multidrug-resistant strains in the planktonic and biofilm state.

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