The effect of diurnal variation on erythrocyte sedimentation rate

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Muammer Yucel ◽  
Alperen Ihtiyar ◽  
Mehmet Koseoglu
Keyword(s):  
Diurnal Variation ◽  
Blood Sample ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Laboratory Tests ◽  
Blood Sampling ◽  
Blood Samples ◽  
Erythrocyte Sedimentation

AbstractObjectivesIt has been known that some laboratory tests showed diurnal variation and were affected by fasting-satiety status. We aimed to examine erythrocyte sedimentation rate (ESR) for both pre-analytical factors.MethodsBlood samples from 12 volunteers were taken and studied with the Westergren method. Fasting blood samples taken at 9:00 am were accepted as basal. Samples were taken at 10:00, 11:00, 12:00 were compared with baseline to evaluate fasting-satiety status and samples taken at 12:00, 15:00, 18:00, and 24:00 to evaluate diurnal variation.ResultsESR was found to be the lowest at 09:00 and 15:00 (Median=5.5 mm/h). The 10:00, 11:00, and 12:00 results for assessing fasting-satiety status on ESR were not different than the baseline (6.2, 6.0, and 7.8 mm/h, respectively). The rates at 18:00 and 24:00 were not found to be different than the baseline (5.7 and 6.6 mm/h).ConclusionsWe didn’t find that ESR had diurnal variation, and it wasn’t affected by fasting-satiety status. Although it is known that routine biochemistry tests should be performed from the blood sample taken in the morning in case of starvation, blood sampling can be done for ESR during the day if necessary.

scholarly journals Microfluidic-Based Biosensor for Blood Viscosity and Erythrocyte Sedimentation Rate Using Disposable Fluid Delivery System

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 215
Author(s):  
Yang Jun Kang
Keyword(s):  
Blood Sample ◽  
Erythrocyte Sedimentation Rate ◽  
Flow Rate ◽  
Sedimentation Rate ◽  
Blood Viscosity ◽  
Blood Samples ◽  
Image Intensity ◽  
Fluid Delivery ◽  
Erythrocyte Sedimentation ◽  
Over Time

To quantify the variation of red blood cells (RBCs) or plasma proteins in blood samples effectively, it is necessary to measure blood viscosity and erythrocyte sedimentation rate (ESR) simultaneously. Conventional microfluidic measurement methods require two syringe pumps to control flow rates of both fluids. In this study, instead of two syringe pumps, two air-compressed syringes (ACSs) are newly adopted for delivering blood samples and reference fluid into a T-shaped microfluidic channel. Under fluid delivery with two ACS, the flow rate of each fluid is not specified over time. To obtain velocity fields of reference fluid consistently, RBCs suspended in 40% glycerin solution (hematocrit = 7%) as the reference fluid is newly selected for avoiding RBCs sedimentation in ACS. A calibration curve is obtained by evaluating the relationship between averaged velocity obtained with micro-particle image velocimetry (μPIV) and flow rate of a syringe pump with respect to blood samples and reference fluid. By installing the ACSs horizontally, ESR is obtained by monitoring the image intensity of the blood sample. The averaged velocities of the blood sample and reference fluid (<UB>, <UR>) and the interfacial location in both fluids (αB) are obtained with μPIV and digital image processing, respectively. Blood viscosity is then measured by using a parallel co-flowing method with a correction factor. The ESR is quantified as two indices (tESR, IESR) from image intensity of blood sample (<IB>) over time. As a demonstration, the proposed method is employed to quantify contributions of hematocrit (Hct = 30%, 40%, and 50%), base solution (1× phosphate-buffered saline [PBS], plasma, and dextran solution), and hardened RBCs to blood viscosity and ESR, respectively. Experimental Results of the present method were comparable with those of the previous method. In conclusion, the proposed method has the ability to measure blood viscosity and ESR consistently, under fluid delivery of two ACSs.

scholarly journals Malaria Infection Prevalence and Haematological Profiles of Nursery and Primary School Children in Fegge, Onitsha, Anambra State-Nigeria

2021 ◽  
pp. 65-84
Author(s):  
A. E. Onyido ◽  
J. O. Esene ◽  
J. U. Anumba ◽  
P. U. Umeanaeto ◽  
C. Ugha ◽  
...  
Keyword(s):  
Blood Cell ◽  
Primary School ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Primary Schools ◽  
Malaria Infection ◽  
Infection Prevalence ◽  
Blood Samples ◽  
Anambra State ◽  
Erythrocyte Sedimentation

A study on malaria infection prevalence and haematological profiles of nursery and primary school pupils aged 0 – 14 years old was conducted in three selected primary schools in Fegge, Onitsha South Local Government Area, Anambra State. Three hundred and sixty (360) pupils were randomly selected from the schools and 2ml of venous blood was collected by venipuncture. Thick and thin blood films were made and stained with Field’s stain A and B. Haematological profiles such as Haemoglobin, White Blood Cell Count, Mean Corpuscular Haemoglobin (Hb) Concentration and Erythrocyte Sedimentation Rate were determined using the anticoagulated blood samples. Of the 360 blood samples examined, 342 (95.0%) comprising 170 (47.22%) males and 172 (47.78%) females were positive for Plasmodium falciparum across the three primary schools. Differences in malaria prevalence among the schools and gender was not statistically significant (P>0.05). Pupils within the age group 3 - 5 and 6 - 8 years recorded the highest infection rate of 118 (96.72%) and 102 (96.22%) respectively which was not statistically significant (P>0.05). The highest percentage of the pupils 10 (55.6%) with heavy malaria intensity had their haemoglobin levels within the lower normal range of 11.0 – 11.9g/dl. There were significant and no significant differences in the haematological profiles of the pupils – Haemoglobin and Packed Cell Volume (P<0.05) indicating mild anaemia, while White Blood Cell Counts (P>0.05) indicates mild leukopenia and for Erythrocyte Sedimentation Rate (P>0.05). The study showed that malaria is holoendemic in Fegge area of Onitsha and poses a significant health problem for the children in the study area. Improved health education in the schools on preventive measures for malaria transmission including integrated vector control to reduce vector-man is recommended.

scholarly journals The Canine Erythrocyte Sedimentation Rate (ESR): Evaluation of a Point-of-Care Testing Device (MINIPET DIESSE)

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Carlo Militello ◽  
Anna Pasquini ◽  
Anyela Andrea Medina Valentin ◽  
Petra Simčič ◽  
Giulia De Feo ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Count ◽  
Point Of Care ◽  
Pearson Correlation ◽  
Analytical Sensitivity ◽  
Predictive Values ◽  
Blood Samples ◽  
Percentile Method ◽  
Erythrocyte Sedimentation

The erythrocyte sedimentation rate (ESR) in canine medicine has been replaced by the evaluation of a few sensitive markers of the acute-phase proteins. The aim of the study was to evaluate the ESR using a point-of-care (MINIPET, DIESSE Diagnostica Senese S.p.A.) device (ESR-MP) and to compare the results with the gold standard Westergren method (ESR-W) in dogs. One hundred and nineteen K3-EDTA blood samples for complete blood count were randomly selected and assayed for ESR. The reference interval (RI) was established using the percentile method. The coefficient of variation (CV) in intra-assay and interassay precision of ESR-MP was calculated. The analytical sensitivity (Se), specificity (Sp), positive predictive values (PPVs), and negative predictive values (NPVs) were calculated. Agreement between ESR-MP and ESR-W was assessed with Pearson correlation coefficient (r), Cohen concordance test (K), Passing-Bablok regression, and Bland–Altman plots. Ten canine samples (8.4%) were ruled out because of flag-error by the MINIPET instrument (4.2%) or because they showed the diphasic pattern in ESR-W (4.2%). The canine RI of ESR-MP was 0–10 mm/h. Precision was excellent in intra-assay (CV = 0.02) and interassay (CV = 0.32). The analytical characteristics of ESR-MP in nonanemic samples were as follows: Se = 0.82, Sp = 0.95, PPV = 0.82, and NPV = 0.95. The accuracy of ESR-MP was better than ESR-W in nonanemic samples (r = 0.87; K = 0.77) and lower in anemic subjects (Hct <37%) (r = 0.76; K = 0.69). The Passing-Bablok regression showed the presence of systematic error and the absence of proportional error only in nonanemic blood samples. The Bland–Altman plots gave negative average values due to the difference in RIs and an agreement in both ESRs. The ESR-MP results can be obtained with the same K3-EDTA tubes used for the blood count, in shortcut time, and at reduced costs using the MINIPET device. These investigations highlight that ESR-MP could be useful in canine clinical settings.

scholarly journals The Influence of Stress on Erythrocyte Sedimentary Rate (ESR) for the Staff of College of Science – Diyala University

2019 ◽  
pp. 71-77 ◽  
Author(s):  
Mohammed A. Farhan ◽  
Wafaa Sh. Al-Zuhairi ◽  
Nuha Abduljaleel Imran
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Normal Level ◽  
Sedimentation Rate ◽  
Working Hours ◽  
Stress Effect ◽  
Blood Samples ◽  
Erythrocyte Sedimentation ◽  
Before And After ◽  
Statistical Program

This study was applied on 15 teaching staffs (10 men and 5 women) from Department of Chemistry, Diyala University to measure the effect of the stress during the working hours on the erythrocyte sedimentation rate (ESR). Blood samples were taken from the teaching staffs before and after the work, as well as another sample after the end of the working hours. The results were analyzed using a statistical program. Most of the results were within the normal level of ESR. There is no stress effect on teaching staffs during the working hours.

DIURNAL VARIATION OF ERYTHROCYTE SEDIMENTATION RATE RELATED TO FEEDING

The Lancet ◽  
1982 ◽  
Vol 319 (8268) ◽  
pp. 389-390 ◽  
Author(s):  
R.K Mallya ◽  
H Berry ◽  
B.E.W Mace ◽  
F.C De Beer ◽  
M.B Pepys
Keyword(s):  
Diurnal Variation ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Erythrocyte Sedimentation

scholarly journals Haematological Indices and Predisposing Factors Associated with Tuberculosis at Federal Medical Center Owerri, Imo State, Nigeria

2020 ◽  
pp. 1-8
Author(s):  
Gideon I. A. Okoroiwu ◽  
Leticia I. Ebere
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Medical Center ◽  
Predisposing Factors ◽  
Tuberculosis Infection ◽  
Blood Samples ◽  
Imo State ◽  
Erythrocyte Sedimentation ◽  
History Of ◽  
Haematological Indices

Introduction: Tuberculosis (TB) is a chronic infectious disease caused by bacterium “Mycobacterium tuberculosis”. It is a systemic infection and has deleterious effect on both circulatory and respiratory systems, and thrives more in a society with little knowledge of its predisposing factors. Aim of the Study: This study was carried out to identify the predisposing factors of tuberculosis and its effects on haematological indices of the positive patients at the Federal Medical Center, Owerri, Imo state, Nigeria. Materials and Methods: The blood samples were analyzed manually for the indices while predisposing factors of Tuberculosis were obtained with the aid of a structured self-administered questionnair,these were administered simultaneously while the blood samples were been collected. Results and Discussion: Results were considered to be statistically signifificant (P<0.05), (OR>1). Simple frequencies were also computed. The results revealed that Haemoglobin (P=0.001); Erythrocyte sedimentation rate (P=0.020); Neutrophils (P=0.002); Eosinophils (P=0.000); Monocytes (P=0.000); Platelets (P=0.001) of the Tuberculosis positive patients when compared with the controls, showed, statistically (P<0.05) significant haematological abnormalities. History of Tuberculosis in family (Odd ratio=9.3) and Alcoholism (OR=6.0) were significant predisposing factors of Tuberculosis. Other predisposing factors; smoking habits (OR=2.7), Educational status (OR=2.2), marital status (OR=1.3) were also associated with Tuberculosis infection, while employment status (OR=1.0) and socio-economic status (OR=0.1) were not. Tuberculosis infection in this study adversely affected Haemoglobin, Platelets, Erythrocyte sedimentation rate, Neutrophil, Eosinophil and Monocyte values, while family history of Tuberculosis and Alcoholism were significantly associated with the infection. Conclusion: Quarrantine of Tuberculosis patients and creating more awareness on the predisposing factors of tuberculosis will help towards preventing, reducing and eliminating the disease.

scholarly journals DIFFERENCES IN LED VALUES IN DENGUE FEVER PATIENTS WITH HEMOCONCENTRATION AND NOT HEMOCONCENTRATION

2019 ◽  
Vol 3 (2) ◽  
pp. 63
Author(s):  
Rismita Pritanti ◽  
Paulus Budiono Notopuro
Keyword(s):  
Blood Sample ◽  
Erythrocyte Sedimentation Rate ◽  
Dengue Fever ◽  
Sedimentation Rate ◽  
Plasma Proteins ◽  
Clinical Pathology ◽  
T Test ◽  
Pathology Laboratory ◽  
Erythrocyte Sedimentation ◽  
The Difference

Background: Patients with dengue fever with hemoconcentration will experience plasma tearing (loss of plasma protein) which will affect the value of the Erythrocyte Sedimentation Rate (LED). Plasma proteins that play a role in influencing the value of the Sedimentation Rate (LED) are fibrinogen and globulins. An erythrocyte sedimentation (LED) examination measures the degree of erythrocyte deposition in a blood sample over a period of time. LEDs are sensitive but not specific tests. Purpose: To determine the difference in LED values of DHF patients with hemoconcentration and not hemoconcentration. Methods: Observational analytic, 30 samples with criteria for dengue fever patients with hemoconcentration and not hemoconcentration were examined at the Clinical Pathology Laboratory of Haji Hospital Surabaya in March - April 2019. The study used an independent t test with SPSS 16.0 program to determine whether there were differences in LED values in dengue fever patients with hemoconcentration and not hemoconsetration. Results: The results of the Independent t test showed that there were significant differences between the results of the erythrocyte sedimentation rate in patients with dengue fever with hemoconcentration and not hemoconsetration with Sig. (2-failed) of 0.00 (p <0.005). Conclusion: There are significant differences in sediment rate results with the Westergreen blood method in patients with dengue fever who experience hemoconcentration and who do not experience hemoconcentration.

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