scholarly journals The Canine Erythrocyte Sedimentation Rate (ESR): Evaluation of a Point-of-Care Testing Device (MINIPET DIESSE)

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Carlo Militello ◽  
Anna Pasquini ◽  
Anyela Andrea Medina Valentin ◽  
Petra Simčič ◽  
Giulia De Feo ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Count ◽  
Point Of Care ◽  
Pearson Correlation ◽  
Analytical Sensitivity ◽  
Predictive Values ◽  
Blood Samples ◽  
Percentile Method ◽  
Erythrocyte Sedimentation

The erythrocyte sedimentation rate (ESR) in canine medicine has been replaced by the evaluation of a few sensitive markers of the acute-phase proteins. The aim of the study was to evaluate the ESR using a point-of-care (MINIPET, DIESSE Diagnostica Senese S.p.A.) device (ESR-MP) and to compare the results with the gold standard Westergren method (ESR-W) in dogs. One hundred and nineteen K3-EDTA blood samples for complete blood count were randomly selected and assayed for ESR. The reference interval (RI) was established using the percentile method. The coefficient of variation (CV) in intra-assay and interassay precision of ESR-MP was calculated. The analytical sensitivity (Se), specificity (Sp), positive predictive values (PPVs), and negative predictive values (NPVs) were calculated. Agreement between ESR-MP and ESR-W was assessed with Pearson correlation coefficient (r), Cohen concordance test (K), Passing-Bablok regression, and Bland–Altman plots. Ten canine samples (8.4%) were ruled out because of flag-error by the MINIPET instrument (4.2%) or because they showed the diphasic pattern in ESR-W (4.2%). The canine RI of ESR-MP was 0–10 mm/h. Precision was excellent in intra-assay (CV = 0.02) and interassay (CV = 0.32). The analytical characteristics of ESR-MP in nonanemic samples were as follows: Se = 0.82, Sp = 0.95, PPV = 0.82, and NPV = 0.95. The accuracy of ESR-MP was better than ESR-W in nonanemic samples (r = 0.87; K = 0.77) and lower in anemic subjects (Hct <37%) (r = 0.76; K = 0.69). The Passing-Bablok regression showed the presence of systematic error and the absence of proportional error only in nonanemic blood samples. The Bland–Altman plots gave negative average values due to the difference in RIs and an agreement in both ESRs. The ESR-MP results can be obtained with the same K3-EDTA tubes used for the blood count, in shortcut time, and at reduced costs using the MINIPET device. These investigations highlight that ESR-MP could be useful in canine clinical settings.

scholarly journals Microfluidic-Based Biosensor for Blood Viscosity and Erythrocyte Sedimentation Rate Using Disposable Fluid Delivery System

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 215
Author(s):  
Yang Jun Kang
Keyword(s):  
Blood Sample ◽  
Erythrocyte Sedimentation Rate ◽  
Flow Rate ◽  
Sedimentation Rate ◽  
Blood Viscosity ◽  
Blood Samples ◽  
Image Intensity ◽  
Fluid Delivery ◽  
Erythrocyte Sedimentation ◽  
Over Time

To quantify the variation of red blood cells (RBCs) or plasma proteins in blood samples effectively, it is necessary to measure blood viscosity and erythrocyte sedimentation rate (ESR) simultaneously. Conventional microfluidic measurement methods require two syringe pumps to control flow rates of both fluids. In this study, instead of two syringe pumps, two air-compressed syringes (ACSs) are newly adopted for delivering blood samples and reference fluid into a T-shaped microfluidic channel. Under fluid delivery with two ACS, the flow rate of each fluid is not specified over time. To obtain velocity fields of reference fluid consistently, RBCs suspended in 40% glycerin solution (hematocrit = 7%) as the reference fluid is newly selected for avoiding RBCs sedimentation in ACS. A calibration curve is obtained by evaluating the relationship between averaged velocity obtained with micro-particle image velocimetry (μPIV) and flow rate of a syringe pump with respect to blood samples and reference fluid. By installing the ACSs horizontally, ESR is obtained by monitoring the image intensity of the blood sample. The averaged velocities of the blood sample and reference fluid (<UB>, <UR>) and the interfacial location in both fluids (αB) are obtained with μPIV and digital image processing, respectively. Blood viscosity is then measured by using a parallel co-flowing method with a correction factor. The ESR is quantified as two indices (tESR, IESR) from image intensity of blood sample (<IB>) over time. As a demonstration, the proposed method is employed to quantify contributions of hematocrit (Hct = 30%, 40%, and 50%), base solution (1× phosphate-buffered saline [PBS], plasma, and dextran solution), and hardened RBCs to blood viscosity and ESR, respectively. Experimental Results of the present method were comparable with those of the previous method. In conclusion, the proposed method has the ability to measure blood viscosity and ESR consistently, under fluid delivery of two ACSs.

scholarly journals Malaria Infection Prevalence and Haematological Profiles of Nursery and Primary School Children in Fegge, Onitsha, Anambra State-Nigeria

2021 ◽  
pp. 65-84
Author(s):  
A. E. Onyido ◽  
J. O. Esene ◽  
J. U. Anumba ◽  
P. U. Umeanaeto ◽  
C. Ugha ◽  
...  
Keyword(s):  
Blood Cell ◽  
Primary School ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Primary Schools ◽  
Malaria Infection ◽  
Infection Prevalence ◽  
Blood Samples ◽  
Anambra State ◽  
Erythrocyte Sedimentation

A study on malaria infection prevalence and haematological profiles of nursery and primary school pupils aged 0 – 14 years old was conducted in three selected primary schools in Fegge, Onitsha South Local Government Area, Anambra State. Three hundred and sixty (360) pupils were randomly selected from the schools and 2ml of venous blood was collected by venipuncture. Thick and thin blood films were made and stained with Field’s stain A and B. Haematological profiles such as Haemoglobin, White Blood Cell Count, Mean Corpuscular Haemoglobin (Hb) Concentration and Erythrocyte Sedimentation Rate were determined using the anticoagulated blood samples. Of the 360 blood samples examined, 342 (95.0%) comprising 170 (47.22%) males and 172 (47.78%) females were positive for Plasmodium falciparum across the three primary schools. Differences in malaria prevalence among the schools and gender was not statistically significant (P>0.05). Pupils within the age group 3 - 5 and 6 - 8 years recorded the highest infection rate of 118 (96.72%) and 102 (96.22%) respectively which was not statistically significant (P>0.05). The highest percentage of the pupils 10 (55.6%) with heavy malaria intensity had their haemoglobin levels within the lower normal range of 11.0 – 11.9g/dl. There were significant and no significant differences in the haematological profiles of the pupils – Haemoglobin and Packed Cell Volume (P<0.05) indicating mild anaemia, while White Blood Cell Counts (P>0.05) indicates mild leukopenia and for Erythrocyte Sedimentation Rate (P>0.05). The study showed that malaria is holoendemic in Fegge area of Onitsha and poses a significant health problem for the children in the study area. Improved health education in the schools on preventive measures for malaria transmission including integrated vector control to reduce vector-man is recommended.

scholarly journals A portable microfluidic system for rapid measurement of the erythrocyte sedimentation rate

Lab on a Chip ◽  
2016 ◽  
Vol 16 (24) ◽  
pp. 4682-4690 ◽  
Author(s):  
Ziya Isiksacan ◽  
Ozcan Erel ◽  
Caglar Elbuken
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Point Of Care ◽  
Microfluidic System ◽  
Erythrocyte Aggregation ◽  
Rate Measurement ◽  
Handheld Device ◽  
Rapid Measurement ◽  
Erythrocyte Sedimentation

A point-of-care handheld device is demonstrated for rapid erythrocyte sedimentation rate measurement using erythrocyte aggregation.

scholarly journals The Influence of Stress on Erythrocyte Sedimentary Rate (ESR) for the Staff of College of Science – Diyala University

2019 ◽  
pp. 71-77 ◽  
Author(s):  
Mohammed A. Farhan ◽  
Wafaa Sh. Al-Zuhairi ◽  
Nuha Abduljaleel Imran
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Normal Level ◽  
Sedimentation Rate ◽  
Working Hours ◽  
Stress Effect ◽  
Blood Samples ◽  
Erythrocyte Sedimentation ◽  
Before And After ◽  
Statistical Program

This study was applied on 15 teaching staffs (10 men and 5 women) from Department of Chemistry, Diyala University to measure the effect of the stress during the working hours on the erythrocyte sedimentation rate (ESR). Blood samples were taken from the teaching staffs before and after the work, as well as another sample after the end of the working hours. The results were analyzed using a statistical program. Most of the results were within the normal level of ESR. There is no stress effect on teaching staffs during the working hours.

scholarly journals Role of Loop Ileostomy in the Management of Ulcerative Colitis Patients

2020 ◽  
Vol 15 (2) ◽  
pp. 62-68
Author(s):  
Ahmed N. Hussein
Keyword(s):  
Ulcerative Colitis ◽  
Erythrocyte Sedimentation Rate ◽  
Medical Therapy ◽  
Sedimentation Rate ◽  
Blood Count ◽  
Complete Blood Count ◽  
Loop Ileostomy ◽  
Erythrocyte Sedimentation ◽  
Fulminant Ulcerative Colitis

Background: Ulcerative colitis is a chronic inflammatory condition characterized by relapsing and remitting episodes of inflammation limited to the mucosal layer of the colon.Loop ileostomy might be helpful in the management of the disease. Objective: To evaluate the role of loop ileostomy in the management of ulcerative colitis.  Methods: Between Januarys 2008 and January 2018, patients with fulminant ulcerative colitis resistant to medical therapy had been referred to our surgical unit and were included in our study. Assessment of the patients’ medical status (serum electrolytes, total serum protein, complete blood count, erythrocyte sedimentation rate, general stool exam,and abdominal ultrasound and x-rays) and psychological statuswere done and then preoperative status correction followed by defunctioning loop ileostomy operated upon them and followed postoperatively in hospital for the response fortwo weeks of hospitalization. Results: Twenty patients were included in the study, two patients died postoperatively due to comorbidities and pulmonary embolism. Eighteen of twenty patients had a gradual improvement clinically (mucus diarrhea with/without blood, weight gain, fever, psychological and edema) and in hematological and biochemicalvalues (complete blood count, serum albumin protein, erythrocyte sedimentation rate and electrolytes) within two weeks. Conclusion: Defunctioning loop ileostomy is a simple,less morbidprocedure if compared to other medical treatments which can change the complication features of fulminant ulcerative colitis towards either continue in medical therapy or towards definitive curative surgery and cost effective as it decrease the need for expensive medications.

The effect of diurnal variation on erythrocyte sedimentation rate

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Muammer Yucel ◽  
Alperen Ihtiyar ◽  
Mehmet Koseoglu
Keyword(s):  
Diurnal Variation ◽  
Blood Sample ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Laboratory Tests ◽  
Blood Sampling ◽  
Blood Samples ◽  
Erythrocyte Sedimentation

AbstractObjectivesIt has been known that some laboratory tests showed diurnal variation and were affected by fasting-satiety status. We aimed to examine erythrocyte sedimentation rate (ESR) for both pre-analytical factors.MethodsBlood samples from 12 volunteers were taken and studied with the Westergren method. Fasting blood samples taken at 9:00 am were accepted as basal. Samples were taken at 10:00, 11:00, 12:00 were compared with baseline to evaluate fasting-satiety status and samples taken at 12:00, 15:00, 18:00, and 24:00 to evaluate diurnal variation.ResultsESR was found to be the lowest at 09:00 and 15:00 (Median=5.5 mm/h). The 10:00, 11:00, and 12:00 results for assessing fasting-satiety status on ESR were not different than the baseline (6.2, 6.0, and 7.8 mm/h, respectively). The rates at 18:00 and 24:00 were not found to be different than the baseline (5.7 and 6.6 mm/h).ConclusionsWe didn’t find that ESR had diurnal variation, and it wasn’t affected by fasting-satiety status. Although it is known that routine biochemistry tests should be performed from the blood sample taken in the morning in case of starvation, blood sampling can be done for ESR during the day if necessary.

Erythrocyte sedimentation rate and C-reactive protein compared in the elderly.

1989 ◽  
Vol 35 (3) ◽  
pp. 466-468 ◽  
Author(s):  
P R Katz ◽  
S I Gutman ◽  
G Richman ◽  
J Karuza ◽  
W R Bartholomew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
The Elderly ◽  
C Reactive Protein ◽  
Predictive Values ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Sensitivity Specificity

Abstract The erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) concentrations were studied in 101 elderly individuals (mean age 72 y) to determine their utility as diagnostic aids in subjects with underlying infection/inflammation. Whereas ESR and CRP were both significantly increased in patients with infection or inflammation, or both, analysis of variance indicated that those subjects still alive six months later had significantly lower ESR values. Analysis of sensitivity, specificity, and positive predictive values indicated that neither test satisfactorily discriminated between patients with and those without ongoing active or chronic disease. Receiver-operating characteristic curve analysis confirmed the low true-positive/false-positive ratios of both ESR and CRP. In the elderly, neither CRP nor ESR has distinct advantages over the other, and both tests evidently have limited utility.

Comparison of K2 and K3 EDTA Anticoagulant on Complete Blood Count and Erythrocyte Sedimentation Rate

2021 ◽  
Vol 28 (1) ◽  
pp. 75
Author(s):  
Harida Zahraini ◽  
Yulia Nadar Indrasari ◽  
Hartono Kahar
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Count ◽  
Complete Blood Count ◽  
Rank Test ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Difference ◽  
Cross Sectional Design

The use of anticoagulants is one of the important pre-analytic factors in hematological tests. Both dipotassium (K2) andtripotassium (K3) Ethylene Diamine Tetraacetic Acid (EDTA) are widely used anticoagulants. International CouncilStandardization of Hematology (ICSH) and several researchers recommend the use of K2 EDTA due to its less hyperosmolareffect on blood cells compared to K3 EDTA. This study aimed to compare the results of Complete Blood Count (CBC) andErythrocyte Sedimentation Rate (ESR) using anticoagulant K2 EDTA and K3 EDTA. This study was an analytic observationalstudy with a cross-sectional design conducted from April to December 2018. The subject of the study were 103 healthyadults selected by consecutive sampling. Blood samples were collected in both anticoagulant tubes with a volume of 3 mLeach. Samples were tested twice, in the first 0 hours and the next 6 hours using Sysmex XN 1000 and Alifax Roller 20 LC.Kolmogorov-Smirnov test, paired T-test and Wilcoxon rank test were used for statistical analysis. The agreement testbetween both anticoagulants was carried out using the Bland Altman plot for parameters with a significant difference. Therewas a significant difference between both anticoagulants for the parameters of hemoglobin, hematocrit MCV, MCHC, RDW,PDW, MPV, PLC-R, and erythrocyte sedimentation rate in both the first and second tests. The agreement test using the BlandAltman plot showed that the difference in these parameters was within the Limit of Agreement (LOA) range of 95%. Thisstudy showed that there were differences in some parameters of complete blood count and erythrocyte sedimentation ratebetween the two anticoagulants (K2 K3 EDTA), but these differences were within the LOA range.

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