Notizen:Untersuchungen über Carotinoide in Gametangien und Sporen von Allomyces arbuscula/ Studies on Carotenoids in Gametangia and Spores of Allomyces arbuscula

1977 ◽  
Vol 32 (5-6) ◽  
pp. 442-443
Author(s):  
P. Fähnrich ◽  
L. Chenaux
Keyword(s):  
Thin Layer ◽  
Visible Light ◽  
Thin Layer Chromatography ◽  
Allomyces Arbuscula ◽  
Lag Period ◽  
Development Analysis ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids were extracted from gametophytes of Allomyces arbuscula at different periods during gametogenesis. After separation by thin-layer chromatography γ-carotene, lycopene and β-carotene were identified from their absorp­tion spectra in visible light. No other carotenoids were detected. After a lag period of 30 min following induction of gametogenesis γ-carotene was the only carotenoid present in extracts, followed by traces of β-carotene at 60 min and by lycopene at 90 min. Accumulation of carotenoids continued during the later stages of gametangia development. Analysis of pigments that had been extracted from asexual mitospores showed that these spores contained both γ-carotene and lycopene.

Carotenoids in Lichens From Tasmanian Forests

1990 ◽  
Vol 38 (5) ◽  
pp. 517 ◽  
Author(s):  
B Czeczuga ◽  
G Kantvilas
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
First Time ◽  
Layer Chromatography ◽  
Β Carotene

Column and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 20 lichen species from Tasmanian forests: α-carotene, β-carotene, β -cryptoxanthin, lutein, zeaxanthin, 5-hydroxy-5,6-dihydrozeaxanthin, β -carotene epoxide, lutein epoxide, antheraxanthin,mutatoxanthin, violaxanthin, neoxanthin, α-doradexanthin, canthaxanthin and astaxanthin. 5-Hydroxy-5,6-dihydrozeaxanthin is reported for the first time in lichens. The total content of carotenoids ranged from 15.28�g-1 dry wt in Menegazzia confusa to 48.53�g-1 dry wt in Pseudocyphellaria glabra.

scholarly journals ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.)

2019 ◽  
Vol 7 (2) ◽  
pp. 104
Author(s):  
Dias Diasasthisa ◽  
Darus J Paransa ◽  
Desy MH Mantiri ◽  
Antonius Rumengan ◽  
Veibe Warouw ◽  
...  
Keyword(s):  
Vitamin A ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Photosynthetic Bacteria ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Bacteria And Fungi ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  

The carotenoids of the marigold, Tagetes erecta

1968 ◽  
Vol 46 (12) ◽  
pp. 1539-1541 ◽  
Author(s):  
A. U. Alam ◽  
J. R. Couch ◽  
C. R. Creger
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spectral Methods ◽  
Tagetes Erecta ◽  
Absorption Studies ◽  
Layer Chromatography ◽  
Β Carotene

Pigments of Tagetes erecta petals have been separated by the use of multiple fractional extractions and thin-layer chromatography and analyzed by spectral methods. The petals showed a predominance of xanthophylls (98.7%) and only a small amount of carotenes. On the basis of absorption studies, lutein (64.1%), antheraxanthin (31.1 %), α-cryptoxanthin (3.1%), (β-carotene (0.6%), phytofluene (0.4%), and α-carotene (0.15%) have been characterized.

scholarly journals Investigations on carotenoids in Embryophyta. IV. The presence of apocarotenals in peatmosses

2014 ◽  
Vol 54 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Dry Weight ◽  
Layer Chromatography ◽  
Β Carotene

By means of column and thin-layer chromatography, the author investigated tit presence of various carotenoids in stems of 3 species of the Sphagnum genus. Apocarotenals (β-apo-2', β-apo-10' -carotenal and apo-12' -violaxanthal) and the following carotenoids were found: α-, β-, ;-carotene, β-cryptoxantin, lutein, lutein epoxide, zeaxanthin, adonixanthin, antheroxanthin, rhodoxanthin, rubixanthin, neoxanthin, vio-laxanthin and mutatoxanthin. The total content of carotenoids ranged from 11.954 to 41.579 mg•g<sup>-1</sup> dry weight.

scholarly journals Invesligations on carotenoids in fungi. IX. Dacrymycetaceae

2014 ◽  
Vol 16 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography ◽  
Β Carotene

The autor used column and thin-layer chromatography to determine the occurrence and quantitative relationships of particular carotenoids in fruit-bodies of the <i>Calocera viscosa</i> and <i>Dacrymyces deliquescens</i>. The predominant carotenoid was β-carotene and its derivatives.

scholarly journals Carotenoids in certain lichens of Białowieża Forest

2014 ◽  
Vol 63 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
Białowieża Forest ◽  
Carotenoid Composition ◽  
Xanthoria Parietina ◽  
Layer Chromatography ◽  
Cladonia Rangiferina ◽  
Β Carotene

Column-, and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 29 lichen species from Białowieża Forest: α-carotene, β-carotene, α-cryptoxanthin, β-cryptoxanthin, lutein, 3'-epilutein, zeaxanthin, β-carotene monoepoxide, lutein epoxide, antheraxanthin, 3'-hydroxyechinenone, α-doradexanthin, canthaxanthin, astaxanthin, neoxani thin, violaxanthin and mutatoxanthin. The total content of carotenoids ranged from 16.83 (<i>Cladonia rangiferina</i>) to 92.98 µg g dry wt (<i>Xanthoria parietina</i>). There were differences in carotenoid composition, concentration of each carotenoid, and in the total content in the thalli of four species collected from niches with different insolation.

Fractionation and Quantification of β-Carotene and Vitamin A Derivatives in Human Serum

1969 ◽  
Vol 15 (6) ◽  
pp. 479-486 ◽  
Author(s):  
Stephan R Targan ◽  
Samuel Merrill ◽  
Arthur D Schwabe
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Thin Layer ◽  
Petroleum Ether ◽  
Human Serum ◽  
Thin Layer Chromatography ◽  
Ultraviolet Spectrophotometry ◽  
Ethanol System ◽  
Layer Chromatography ◽  
Β Carotene

Abstract Concentrations of β-carotene, retinyl esters, retinol, retinal, and retinoic acid were determined quantitatively in human serum. Extraction of the compounds was accomplished with a petroleum ether-ethanol system and fractionation by thin-layer chromatography. Each fraction's absorbance was measured by ultraviolet spectrophotometry and expressed as micrograms per 100 ml. By this method, 85% or more of individual premeasured standards and more than 69% of premeasured combined standards could be recovered. The levels of β-carotene and Vitamin A derivatives recovered from the serums of 10 males and 10 females compared favorably with those obtained with other methods. The procedure outlined is rapid, simple, and reproducible.

Carotenoids in Some Lichen Species from Ireland

1989 ◽  
Vol 21 (4) ◽  
pp. 363-367 ◽  
Author(s):  
B. Czeczuga ◽  
D. H. S. Richardson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
Layer Chromatography ◽  
Β Carotene

AbstractColumn and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of seven lichen species from Ireland: α-carotene, β-carotene, β-cryptoxanthin, lutein, 3'-epilutein, adonixanthin, α-doradexanthin, canthaxanthin, astaxanthin, antheraxanthin, lutein epoxide, neoxanthin and violaxanthin. The total content of carotenoids ranged from 11.9 μg g−1 dry wt in Menegazzia terebrata to 21·3 μg g−1 dry wt in Usnea rubicunda.

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