scholarly journals ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.)

2019 ◽  
Vol 7 (2) ◽  
pp. 104
Author(s):  
Dias Diasasthisa ◽  
Darus J Paransa ◽  
Desy MH Mantiri ◽  
Antonius Rumengan ◽  
Veibe Warouw ◽  
...  
Keyword(s):  
Vitamin A ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Photosynthetic Bacteria ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Bacteria And Fungi ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  

Fractionation and Quantification of β-Carotene and Vitamin A Derivatives in Human Serum

1969 ◽  
Vol 15 (6) ◽  
pp. 479-486 ◽  
Author(s):  
Stephan R Targan ◽  
Samuel Merrill ◽  
Arthur D Schwabe
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Thin Layer ◽  
Petroleum Ether ◽  
Human Serum ◽  
Thin Layer Chromatography ◽  
Ultraviolet Spectrophotometry ◽  
Ethanol System ◽  
Layer Chromatography ◽  
Β Carotene

Abstract Concentrations of β-carotene, retinyl esters, retinol, retinal, and retinoic acid were determined quantitatively in human serum. Extraction of the compounds was accomplished with a petroleum ether-ethanol system and fractionation by thin-layer chromatography. Each fraction's absorbance was measured by ultraviolet spectrophotometry and expressed as micrograms per 100 ml. By this method, 85% or more of individual premeasured standards and more than 69% of premeasured combined standards could be recovered. The levels of β-carotene and Vitamin A derivatives recovered from the serums of 10 males and 10 females compared favorably with those obtained with other methods. The procedure outlined is rapid, simple, and reproducible.

scholarly journals PIGMEN KAROTENOID PADA KEPITING Ozius sp

2020 ◽  
Vol 8 (1) ◽  
pp. 47
Author(s):  
Sriyati Enjelina Sibarani ◽  
Darus Saadah Johanis Paransa ◽  
Kurniati Kemer ◽  
Desy M.H Mantiri ◽  
Natalie D.C Rumampuk ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Body ◽  
Metabolic Process ◽  
Natural Dyes ◽  
Separation Method ◽  
Carotenoid Pigments ◽  
Eye Health ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoid pigments are natural dyes that have an influence on organism. For the human body, carotenoids function as antioxidants, anticancer, anti-bacteria and help maintain eye health. Carotenoid pigments are found in groups of bacteria, fungi, molds, algae and green plants and one of them in crustaceans is the crab Ozius sp. To determine the metabolic process of carotenoid pigments, each crab organ of Ozius sp. isolated using thin layer chromatography separation method to determine the type of pigment contained therein. The type of pigment identified in the crab Ozius sp. females with the TLC method namely: β-carotene, Cantanxhantine, and β-cryptosanthin.Keywords: Ozius sp, Thin Layer Chromatography, Carotenoids Pigments. Pigmen karotenoid adalah zat warna alami yang memiliki pengaruh terhadap organisme. Bagi tubuh manusia karotenoid berfungsi sebagai antioksidan, antikanker, anti bakteri dan membantu memelihara kesehatan mata. Pigmen karotenoid banyak ditemukan pada kelompok bakteri, jamur, kapang, ganggang dan tanaman hijau serta pada krustasea salah satunya yaitu pada kepiting Ozius sp. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Ozius sp. betina dengan metode KLT yaitu: β- karoten, Kantasantin, dan β- kriptosantin.Kata Kunci: Ozius sp, Kromatografi Lapis Tipis, Pigmen Karotenoid

Notizen: Isolation of Batatasin I from Dioscorea dumetorum Rhizomes

1979 ◽  
Vol 34 (3-4) ◽  
pp. 288-289 ◽  
Author(s):  
M. M. El-Olemy ◽  
J. Reisch
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Spectral Evidence ◽  
Layer Chromatography

Abstract Batatasin I was shown to occur in the fresh rhizomes of poisonous yam (Dioscorea dumetorum Pax.,). It was isolated by column chromatography and identified by chromatographic and spectral evidence. Two other unknown phenanthrenes were shown to occur by thin-layer chromatography.

Carotenoids in Lichens From Tasmanian Forests

1990 ◽  
Vol 38 (5) ◽  
pp. 517 ◽  
Author(s):  
B Czeczuga ◽  
G Kantvilas
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
First Time ◽  
Layer Chromatography ◽  
Β Carotene

Column and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 20 lichen species from Tasmanian forests: α-carotene, β-carotene, β -cryptoxanthin, lutein, zeaxanthin, 5-hydroxy-5,6-dihydrozeaxanthin, β -carotene epoxide, lutein epoxide, antheraxanthin,mutatoxanthin, violaxanthin, neoxanthin, α-doradexanthin, canthaxanthin and astaxanthin. 5-Hydroxy-5,6-dihydrozeaxanthin is reported for the first time in lichens. The total content of carotenoids ranged from 15.28�g-1 dry wt in Menegazzia confusa to 48.53�g-1 dry wt in Pseudocyphellaria glabra.

Notizen:Untersuchungen über Carotinoide in Gametangien und Sporen von Allomyces arbuscula/ Studies on Carotenoids in Gametangia and Spores of Allomyces arbuscula

1977 ◽  
Vol 32 (5-6) ◽  
pp. 442-443
Author(s):  
P. Fähnrich ◽  
L. Chenaux
Keyword(s):  
Thin Layer ◽  
Visible Light ◽  
Thin Layer Chromatography ◽  
Allomyces Arbuscula ◽  
Lag Period ◽  
Development Analysis ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids were extracted from gametophytes of Allomyces arbuscula at different periods during gametogenesis. After separation by thin-layer chromatography γ-carotene, lycopene and β-carotene were identified from their absorp­tion spectra in visible light. No other carotenoids were detected. After a lag period of 30 min following induction of gametogenesis γ-carotene was the only carotenoid present in extracts, followed by traces of β-carotene at 60 min and by lycopene at 90 min. Accumulation of carotenoids continued during the later stages of gametangia development. Analysis of pigments that had been extracted from asexual mitospores showed that these spores contained both γ-carotene and lycopene.

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