Isolation of a b-Type Cytochrome Oxidase from Membranes of the Phototrophic Bacterium Rhodopseudomonas capsulata
1982 ◽
Vol 37
(3-4)
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pp. 193-198
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Keyword(s):
Triton X
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Abstract A cytochrome oxidase (EC 1.9.3.1) was solubilized from the membrane fraction of aerobically grown cells of Rhodopseudomonas capsulata by treatment with Triton X-100. The enzyme was purified 160 fold by chromatography on DEAE-Sepharose CL-6B and affinity chromatography on cytochrome c-thiol activated Sepharose 4B.The purified enzyme has a pH-optimum at 8.5 and a temperature optimum at 35 °C. The ap parent Km for reduced horse cytochrome c is 24 μм (at pH 8 and 30 °C). The purified cytochrome oxidase was 50% inhibited by 1.5 μм KCN and 10 μм NaN3. The purified enzyme contained one polypeptide of mr 65,000 and 6-type cytochrome.
1984 ◽
Vol 39
(1-2)
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pp. 68-72
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Reaktionen der Hydrogenase aus Rhodopseudomonas capsulata im partikelgebundenen und gelösten Zustand
1969 ◽
Vol 24
(5)
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pp. 603-612
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1989 ◽
Vol 256
(3)
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pp. E386-E391
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Keyword(s):
Keyword(s):
1982 ◽
Vol 700
(2)
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pp. 184-191
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1985 ◽
Vol 54
(02)
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pp. 533-538
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