Notizen: In vivo Metabolism of [4-13C]Phenacetin in an Isolated Perfused Rat Liver Measured by Continuous Flow 13C NMR Spectroscopy

1984 ◽  
Vol 39 (7-8) ◽  
pp. 859-862 ◽  
Author(s):  
Klaus Albert ◽  
Gerd Kruppa ◽  
Klaus-Peter Zeller ◽  
Ernst Bayer ◽  
Franz Hartmann
Keyword(s):  
Nmr Spectroscopy ◽  
Continuous Flow ◽  
13C Nmr Spectroscopy ◽  
Point Of View ◽  
Isolated Perfused Rat Liver ◽  
Metabolite Formation ◽  
Non Invasive ◽  
First Time ◽  
C Nmr

Abstract Metabolism, [4-13C]Phenacetin, Continuous Flow 13C NMR Continuous flow 13C NMR spectroscopy has been used for the first time to monitor the metabolism of a 13C labeled drug in an isolated liver. Continuous and almost immediate information on the metabolite formation could be obtained using 13C labeled phenacetin without alteration of the biological system. The data are consistent with those observed by conventional techniques (HPLC, aliquot 13C NMR measurements). From the biological point of view the sensitivity of continuous flow 13C NMR spectroscopy is still low (10-3 ᴍ). The results presented demonstrate however that non-invasive and non-radio-active real time monitoring of drug metabolism in intact organs is possible.

scholarly journals NEW PHARMACOLOGICALLY ACTIVE COMPOUNDS BASED ON 5-HYDROXY-7-METHOXY-2-PHENYLCHROMAN-4-ONE

2021 ◽  
Vol 3 ◽  
pp. 119-127
Author(s):  
G.M. Baisarov ◽  
◽  
S.M. Adekenov ◽  
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr ◽  
Potassium Carbonate ◽  
13C Nmr Spectroscopy ◽  
1H And 13C Nmr ◽  
Pharmacologically Active ◽  
Pharmacologically Active Compounds ◽  
First Time

The reaction of 5-hydroxy-7-methoxy-2-phenylchroman-4-one with dibromoalkanes in acetone in the presence of potassium carbonate proceeds according to the Michael’s retro-reaction O-alkylation and leads to the formation of the corresponding 2-(bromo-alkoxy) chalcones. The structure of the synthesized compounds was confirmed by IR-, 1H- and 13C-NMR spectroscopy. The cytotoxic, hepatoprotective and anti-inflammatory effects of chalcone derivatives (2-3) were studied for the first time in vitro and in vivo.

A Novel Method for 13C-NMR Spectroscopy of Polymer in Emulsion: Quantitative Analysis of Microstructure of Crosslinked Polybutadiene in Latex

1999 ◽  
Vol 72 (5) ◽  
pp. 844-853 ◽  
Author(s):  
Seiichi Kawahara ◽  
Saori Bushimata ◽  
Takashi Sugiyama ◽  
Chihiro Hashimoto ◽  
Yasuyuki Tanaka
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
13C Nmr ◽  
Lattice Relaxation ◽  
13C Nmr Spectroscopy ◽  
Lattice Relaxation Time ◽  
Solid Sample ◽  
Spin Lattice Relaxation ◽  
High Resolution Spectrum ◽  
C Nmr

Abstract A novel analytical method using high resolution 13C-NMR spectroscopy to study polymer latex, which is a heterogeneous system comprising polymer dispersoid and water, is described. The appropriate concentrations of surfactant and dried rubber content of a polybutadiene latex, which give the best spectrum, were found to be 1 w/v % and 10%, respectively. The half-widths of resonance peaks for the latex sample were almost identical to the half-widths obtained in solution, which were about one-third the width of those obtained using a solid sample of either a crosslinked or soluble polybutadiene. Nevertheless, the signal to noise ratio for the latex sample was slightly smaller than that for the solution sample. The values of spin-lattice relaxation time, T1, for the latex sample were similar to those for the solid sample. These demonstrate that the latex state C-NMR spectroscopy will be a powerful technique for structural characterization of crosslinked gels in the dispersoid because it gives a high resolution spectrum comparable to solution state spectroscopy, showing short T1 values corresponding to those for solid state C-NMR spectroscopy. High resolution latex state 13C-NMR spectroscopy was used to determine the concentrations of the various isomers of the polybutadiene in the dispersoid.

scholarly journals New Kaempferol 3,7-Diglycosides from Asplenium ruta-muraria and Asplenium altajense

2015 ◽  
Vol 10 (3) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Tsukasa Iwashina ◽  
Junichi Kitajima ◽  
Takayuki Mizuno ◽  
Sergey V. Smirnov ◽  
Oyunchimeg Damdinsuren ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Adjacent Area ◽  
Altai Mountains ◽  
A Minor ◽  
First Time ◽  
Minor Compound ◽  
C Nmr

A flavonoid was isolated from the fronds of Asplenium ruta-muraria and A. altajense (Aspleniaceae) collected in the Altai Mountains and adjacent area. The compound was identified as kaempferol 3- O- β-[(6″′- E-caffeoylglucopyranosyl)-(1→3)-glucopyranoside]-7- O- β-glucopyranoside (1) by UV, 1H and 13C NMR spectroscopy, LC-MS, and acid and alkaline hydrolyses. Another flavonoid (2) was isolated from A. altajense, as a minor compound, together with 1 and identified as deacylated compound 1, i.e. kaempferol 3- O-laminaribioside-7- O-glucoside. They were found in nature for the first time.

Extent of intramolecular cyclization in RAFT-synthesized methacrylic branched copolymers using 13C NMR spectroscopy

2015 ◽  
Vol 6 (7) ◽  
pp. 1143-1149 ◽  
Author(s):  
J. Rosselgong ◽  
S. P. Armes
Keyword(s):  
Nmr Spectroscopy ◽  
Intramolecular Cyclization ◽  
13C Nmr ◽  
13C Nmr Spectroscopy ◽  
Branched Copolymers ◽  
C Nmr

The extent of intramolecular cyclization for a series of soluble branched methacrylic copolymers is determined by 13C NMR spectroscopy via deconvolution of oxymethylene carbon signals.

Ethylene Insertion with Soluble Ziegler Catalysts: Direct Insight into the Reaction Using Enriched 13C2 H4 and 13C NMR Spectroscopy II. The System Cp2TiMeCl/AlMeCl2/13C2H4

1985 ◽  
Vol 40 (2) ◽  
pp. 158-166 ◽  
Author(s):  
Gerhard Fink ◽  
Wolfgang Fenzl ◽  
Richard Mynott
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr ◽  
13C Nmr Spectroscopy ◽  
Elementary Processes ◽  
Carbon Bond ◽  
Spectroscopic Evidence ◽  
Oligomer Distribution ◽  
Insight Into ◽  
C Nmr

Abstract The elementary processes involved in the polymerization of ethylene by soluble Ziegler catalysts of the type Cp2TiMeCl/AlMeCl2 have been studied by 13C NMR spectroscopy. Using 13C-enriched ethylene, detailed information was obtained directly on the system in the course of the polymerization. No spectroscopic evidence was found for precoordination of the monomer. The development of the oligomer distribution, which could be followed spectroscopically, proves that the mechanism proposed by Olivé cannot be correct. These experiments show conclusively that the ethylene is inserted into the titanium-carbon bond.

scholarly journals Chemical Composition of the Essential Oils from Two Vietnamese Asarum Species: A. glabrum and A. cordifolium

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tran Huy Thai ◽  
Ophélie Bazzali ◽  
Tran Minh Hoi ◽  
Nguyen Anh Tuan ◽  
Félix Tomi ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nmr Spectroscopy ◽  
Endangered Species ◽  
Essential Oil ◽  
Essential Oils ◽  
Spectroscopic Techniques ◽  
Red Data Book ◽  
Data Book ◽  
First Time ◽  
C Nmr

The essential oil isolated from Asarum cordifolium C. E. C. Fischer recently discovered in Vietnam, and A. glabrum Merr., an endangered species listed as vulnerable in the Red Data Book of Vietnam, have been analyzed by a combination of chromatographic and spectroscopic techniques including 13C NMR spectroscopy. The composition of A. cordifolium essential oil, investigated for the first time, was dominated by elemicin (82.5%). The essential oil isolated from A. glabrum contained safrole (41.9%) as its major component and was characterized by the diversity of phenylpropanoids contained in this oil (10 compounds).

Neue Untersuchung über Inhaltsstoffe der Kreuzdornrinde, IV [1] Die Hauptanthraglykoside der Rinde von Rhamnus catharticus L.: Emodin-8-O-β-gentiobiosid, -glukosid und -primverosid / A New Investigation on the Com position of Rhamni cathartici Cortex, IV [1] The Main Anthraglycosides of the Stem Bark of Rhamnus catharticus L.: Emodin-8-0-/β-gentiobioside, -glucoside and -primveroside

1983 ◽  
Vol 38 (3-4) ◽  
pp. 170-178 ◽  
Author(s):  
Hans-W. Rauwald
Keyword(s):  
Nmr Spectroscopy ◽  
Plant Material ◽  
Chemical Shifts ◽  
Stem Bark ◽  
New Method ◽  
Countercurrent Chromatography ◽  
Anthraquinone Glycosides ◽  
Anthraquinone Derivatives ◽  
First Time ◽  
C Nmr

From the stem bark of Rhamnus catharticus L. (purging buckthorn) the three main anthraquinone glycosides have been isolated by the new method of droplet-countercurrent-chromatography. Their structures were spectroscopically identified as em odin-8-O-β-gentiobioside, -glucoside and -primveroside. From these the primveroside is a new substance, the gentiobioside has been found for the second time in plant material. The structures were confirmed by 13C NMR spectroscopy for the first time in O-glycosides of emodin type. Especially the differences in 13C chemical shifts of the free resp. acetylated aglycones and glycosides are useful parameters for determining the position of O-glycosylation in the 1,8-dihydroxy-anthraquinone derivatives

Cerebral glucose metabolism and the glutamine cycle as detected by in vivo and in vitro 13C NMR spectroscopy

2004 ◽  
Vol 45 (2-3) ◽  
pp. 297-303 ◽  
Author(s):  
Marı́a A Garcı́a-Espinosa ◽  
Tiago B Rodrigues ◽  
Alejandra Sierra ◽  
Marina Benito ◽  
Carla Fonseca ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Glucose Metabolism ◽  
13C Nmr ◽  
13C Nmr Spectroscopy ◽  
Cerebral Glucose Metabolism

Structural analysis of hardwood native lignins by quantitative 13C NMR spectroscopy

Holzforschung ◽  
2016 ◽  
Vol 70 (2) ◽  
pp. 95-108 ◽  
Author(s):  
Mikhail Yu. Balakshin ◽  
Ewellyn A. Capanema ◽  
Ricardo B. Santos ◽  
Hou-min Chang ◽  
Hasan Jameel
Keyword(s):  
Nmr Spectroscopy ◽  
Sugar Content ◽  
13C Nmr Spectroscopy ◽  
Structural Variations ◽  
Wide Range ◽  
Structural Peculiarities ◽  
Quantitative 13C Nmr Spectroscopy ◽  
Degree Of Condensation ◽  
G Ratio ◽  
C Nmr

Abstract Milled wood lignins from alkaline pretreated wood with very low sugar content and a wide range of syringyl-to-guaiacyl (S/G) ratio between 1.2 and 3.0 were isolated from 12 industrially valuable hardwood (HW) species. The lignin preparations were investigated by means of a comprehensive 13C nuclear magnetic resonance (NMR) methodology to address the possibilities and limitations of this approach for HW native lignins and to estimate the structural variations within HW lignins. Good correlations were found for different independent methods for the quantification of major lignin moieties. The results were reliable at the C6 level and not only for relative comparison. The correlation was good between methoxyl group determinations by wet chemistry and those by 13C NMR spectroscopy. The limitations of the 13C NMR method were also pointed out. The differences in the S/G ratios can be large, but other structural deviations are less significant. Strong correlations between the S/G ratios and the amounts of other structural peculiarities could not be found by the 13C NMR approach. However, with increasing S/G ratios, the β-O-4 content showed increasing tendencies and the degree of condensation showed decreasing tendencies.

Organoboranes and tetraorganoborates studied by 11B and 13C NMR spectroscopy and DFT calculations

2015 ◽  
Vol 70 (6) ◽  
pp. 421-424 ◽  
Author(s):  
Bernd Wrackmeyer
Keyword(s):  
Nmr Spectroscopy ◽  
Dft Calculations ◽  
13C Nmr ◽  
Quantum Chemical ◽  
13C Nmr Spectroscopy ◽  
Coupling Constants ◽  
Line Widths ◽  
Nmr Signals ◽  
C Nmr ◽  
Extract Information

AbstractCare should be taken on recording the sometimes elusive 13C NMR signals for boron-bonded carbon atoms, since it is easy to extract information about coupling constants 1J(13C,11B) by measuring the respective line widths of 13C(B-C) and 11B NMR signals. This information can be confirmed by quantum-chemical calculations [B3LYP (6-311+G(d,p) level of theory] of nJ(13C,11B) in organoboranes and tetraorganoborates. For the latter, the signs for n = 2, 3, 4 were experimentally determined.

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