Disturbing Gtp-Binding Protein Function Through Microinjection Into The Visual Cell Of Limulus
1992 ◽
Vol 47
(11-12)
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pp. 915-921
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We have tested the action of three agents microinjected into the ventral nerve photoreceptor of Limulus on the electrical response to dim light. 1. A monoclonal antibody (mAb 4 A) against the Gɑ subunit of frog transducin reduces the size of the receptor current to 60%, suggesting an interaction with Gɑ in the Limulus photoreceptor. 2. Injection of Clostridium botulinum ADPribosyltransferase C 3 reduces the size to 46%; latency is not affected. The results imply that small GTP-binding proteins play a functional role in photoreception of invertebrates. 3. Injection of GD P-β-S reduces dose-dependently the size of the receptor current to 15% and prolongs the latency to 200%, presumably by reducing number and rate of G-protein activations
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2003 ◽
Vol 6
(4)
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pp. 399-407
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1993 ◽
Vol 268
(12)
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pp. 8899-8907
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1990 ◽
Vol 265
(5)
◽
pp. 2671-2676
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1991 ◽
Vol 180
(2)
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pp. 615-622
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