Association of an Antibody-Conjugated Enzyme with Synthetic Polymers Dissolved in Organic Solvents

1994 ◽  
Vol 49 (11-12) ◽  
pp. 891-893
Author(s):  
M. Kumakura
Keyword(s):  
Tumor Marker ◽  
Organic Solvents ◽  
Synthetic Polymers ◽  
Polymerization Degree

An antibody of a tumor marker (α-fetoprotein) conjugated with peroxidase was dispersed in organic solvents including synthetic polymers, and the interaction of the antibody with the polymers was investigated. It was found that the antibody-conjugated enzyme is associated with the polymers dissolved in hydrophobic organic solvents. The association correlated with the polymerization degree, concentration, and nature of the polymers.

Hollow-Fiber Flow Field-Flow Fractionation of Synthetic Polymers in Organic Solvents

2001 ◽  
Vol 73 (19) ◽  
pp. 4736-4742 ◽  
Author(s):  
M. van Bruijnsvoort ◽  
W. Th. Kok ◽  
R. Tijssen
Keyword(s):  
Flow Field ◽  
Organic Solvents ◽  
Hollow Fiber ◽  
Synthetic Polymers ◽  
Field Flow Fractionation ◽  
Flow Fractionation

Extraction and identification of fillers and pigments from pyrolyzed rubber and tire samples

1996 ◽  
Vol 54 ◽  
pp. 174-175
Author(s):  
P. Sadhukhan ◽  
J. B. Zimmerman
Keyword(s):  
Zinc Oxide ◽  
Electron Microscope ◽  
Carbon Black ◽  
Natural Rubber ◽  
Transmission Electron Microscope ◽  
Rolling Resistance ◽  
Synthetic Polymers ◽  
Nitrogen Atmosphere ◽  
Transmission Electron ◽  
Curing Agents

Rubber stocks, specially tires, are composed of natural rubber and synthetic polymers and also of several compounding ingredients, such as carbon black, silica, zinc oxide etc. These are generally mixed and vulcanized with additional curing agents, mainly organic in nature, to achieve certain “designing properties” including wear, traction, rolling resistance and handling of tires. Considerable importance is, therefore, attached both by the manufacturers and their competitors to be able to extract, identify and characterize various types of fillers and pigments. Several analytical procedures have been in use to extract, preferentially, these fillers and pigments and subsequently identify and characterize them under a transmission electron microscope.Rubber stocks and tire sections are subjected to heat under nitrogen atmosphere to 550°C for one hour and then cooled under nitrogen to remove polymers, leaving behind carbon black, silica and zinc oxide and 650°C to eliminate carbon blacks, leaving only silica and zinc oxide.

CENP-C: An oncogene or tumor suppressor gene? A possible new tumor marker

2001 ◽  
Vol 120 (5) ◽  
pp. A299-A299
Author(s):  
D KAZANOV ◽  
B STERN ◽  
W PYERIN ◽  
O BOECHER ◽  
H STRUL ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Tumor Marker ◽  
Tumor Suppressor Gene ◽  
Suppressor Gene

Immunoscintigraphy and SPECT in Patients with Pancreatic Carcinom

1985 ◽  
Vol 24 (05) ◽  
pp. 232-237 ◽  
Author(s):  
R Klapdor ◽  
B. Kremer ◽  
B. Rothe ◽  
R Montz ◽  
Keyword(s):  
Tumor Marker ◽  
Kolorektale Karzinome

ZusammenfassungAuf der Basis ermutigender Veröffentlichungen über die Immunszintigraphie bei kolorektalen Karzinomen sowie eigener immunhistochemischer und immunszintigraphischer Befunde an Pankreaskarzinom-Transplantaten auf der Nacktmaus untersuchten wir die diagnostischen Möglichkeiten mit 131J-markierten Antikörpern gegen die Tumor-Marker CA 19-9 und CEA bei 21 Patienten mit Pankreaskarzinom. Zum Vergleich dienten die Untersuchungsergebnisse von weiteren 10 Patienten mit kolorektalem Karzinom, 2 Patienten mit Gallengangs- und 1 mit Magen-Karzinom. Planare Szintigramme der Verdachtsregionen in 2-4 Sichten wurden mehrfach bis 6 Tage nach i.v. Infusion von 2 mCi eines Cocktails aus 131J-anti-CA 19-9 und -anti-CEA aufgenommen. SPECT wurde außerdem 3-4 Tage p.i., zuletzt auch früher, durchgeführt. Primäre Tumoren und Metastasen im Oberbauch waren wegen geringerer Tumor/Umgebungs-Relation der Traceraktivität schwieriger nachzuweisen und zu lokalisieren als kolorektale Karzinome im Unterbauch. Die Tumorabgrenzung wurde in planaren Szintigrammen meist erst 5-6 Tage p.i. oder später erkennbar. SPECT wies dieselben Befunde bereits 3 Tage p. i. oder früher nach. Die Lokalisation und die topographische Zuordnung gelang mit SPECT leichter und häufiger, somit der Nachweis sicherer und empfindlicher. Größere Tumoren stellten sich auch in Fällen ohne erhöhte Serumkonzentration der Tumormarker dar. Der Immunszintigraphie des Pankreaskarzinoms und anderer Karzinommanifestationen im Oberbauch kommt gegenwärtig eine eingeschränkte diagnostische Bedeutung zu; methodische Verbesserungen erscheinen notwendig und möglich.

UNTERSUCHUNGEN ÜBER DIE EXTRAKTION DES ROTEN KOBER-FARBSTOFFS DURCH ORGANISCHE LÖSUNGSMITTEL ZUR ÖSTROGENBESTIMMUNG IM HARN

1960 ◽  
Vol XXXV (I) ◽  
pp. 34-48 ◽  
Author(s):  
Gerd Ittrich
Keyword(s):  
Menstrual Cycle ◽  
Organic Solvents ◽  
Urine Sample ◽  
Phenol Derivatives ◽  
Organische Lösungsmittel ◽  
Colour Complex

ABSTRACT A series of organic solvents and phenol derivatives have been examined for the extraction of the pink Kober-colour complex. Optimal results could be achieved for fluorimetry by a solution of 2 % (w/v) p-nitrophenol and 1 % (v/v) ethanol in acetylenetetrabromide, when the green mercury line (546 mμ was used as primary light. The sensitivity, stability and specificity have been improved, compared with the previously described reaction. By changing the sequence of purification steps and by reducing the volume of the urine sample (5 ml) the method for the determination of total oestrogens has been simplified. Approximately 10 determinations can be done within 3–4 hours by one person. Recovery experiments and comparative determinations with a previously described method have been carried out. The excretion of total oestrogens in a complete menstrual cycle is determined with the described method.

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