Anthocyanins, Colour and Antioxidant Properties of Eggplant (Solanum melongena L.) and Violet Pepper (Capsicum annuum L.) Peel Extracts

2006 ◽  
Vol 61 (7-8) ◽  
pp. 527-535 ◽  
Author(s):  
Eva Sadilova ◽  
Florian C. Stintzing ◽  
Reinhold Carle
Keyword(s):  
Antioxidant Capacity ◽  
Capsicum Annuum ◽  
Nutritional Value ◽  
Antioxidant Properties ◽  
Solanum Melongena ◽  
In Vitro Assays ◽  
Capsicum Annuum L ◽  
Food Colour ◽  
Acetone Extracts

Acetone extracts from eggplant (Solanum melongena L.) and violet pepper (Capsicum annuum L.) peels both belonging to the Solanaceae plant family were characterized with respect to their anthocyanin profiles, colour qualities and antioxidant capacities. According to HPLC-DAD-MS3 analyses the major anthocyanin in eggplant was delphinidin-3-rutinoside, while the predominant pigment in violet pepper was assigned to delphinidin-3-transcoumaroylrutinoside- 5-glucoside. Since virtually all anthocyanins were delphinidin-based, the effect of acylation and glycosylation patterns on colour stability and antioxidant capacity could be assessed. Application of two in vitro-assays for antioxidant capacity assessment revealed that eggplant generally exhibited higher values compared to violet pepper which was ascribed to 3,5-diglycosylated structures predominating in the latter. The higher extent of acylation in violet pepper was reflected by a more purplish colour shade of the extracts, but did not translate into a higher stability against fading which again was attributed to additional glycosyl substitution at C5. These findings support the relevance of structurerelated activities of anthocyanins both for understanding food colour and their particular nutritional value.

scholarly journals Mangiferin Rich Products from Aphloia theiformis (Vahl) Benn Leaves: Extraction, Fractionation, Phytochemical Characterization, and Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2081
Author(s):  
Dovilė Grauzdytė ◽  
Audrius Pukalskas ◽  
Chaker El Kalamouni ◽  
Petras Rimantas Venskutonis
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Liquid Extraction ◽  
In Vitro Assays ◽  
Synthetic Antioxidant ◽  
Liquid Liquid Extraction ◽  
Extraction Solvents ◽  
First Time

Aphloia theiformis is traditionally used in Mauritius, Madagascar, and Reunion Island for treating several diseases. In this study, various extraction solvents and schemes were applied for the recovery of antioxidant rich fractions from the leaves of A. theiformis. The products were evaluated for their antioxidant capacity using well known in vitro assays. Major compounds were characterized by UPLC–QTOF–MS. Hydrophilic extracts of A. theiformis demonstrated strong antioxidant properties, which are comparable with the synthetic antioxidant Trolox. UPLC analysis confirmed mangiferin as the main secondary metabolite of A. theiformis. Tormentic and hydroxytormentic acids as well as their isomers were also abundant in A. theiformis extracts and fractions, while their amounts were determined for the first time. The most potential extract was further separated into the fractions by liquid-liquid extraction and by precipitation at low temperature. Antioxidant capacity and composition of secondary metabolites of derived fractions were determined. Some of the fractions possessed remarkable antioxidant capacity, comparable to pure mangiferin. The results obtained reveal high potential of A. theiformis for recovery of natural antioxidants and other bioactive phytochemicals, particularly mangiferin.

scholarly journals The Amendment with Chitin and/or Chitosan Improves the Germination and Growth of Lycopersicon esculentum L., Capsicum annuum L. and Solanum melongena L.

2020 ◽  
Author(s):  
Rkhaila Amine ◽  
El Hartiti Abla ◽  
Bouziani Idrissi Mohammed ◽  
Ounine Khadija
Keyword(s):  
Lycopersicon Esculentum ◽  
Capsicum Annuum ◽  
Solanum Melongena ◽  
Germination Percentage ◽  
In Vivo Test ◽  
Capsicum Annuum L ◽  
Chitin And Chitosan ◽  
Germination And Growth

Background: Chitin and chitosan are natural products extracted from shrimp shells. They are non-toxic, biocompatible and biodegradable which favors many applications in agricultural domain. Moreover, chitin and chitosan are known as stimulators of photosynthetic rates and enhance pants growth through minerals contains (C, O, N ….). In the present study, these co-products are evaluated for their ability to stimulate the germination and growth of Lycopersicon esculentum L., Capsicum annuum L. and Solanum melongena L. Methods: The experiments were conducted at Ibn Tofail University-Kenitra, between July 2016 to January 2018. The experiments are carried out by using a germination test for the three seeds. On the other hand, the in vivo test is used to determine the effect of the biopolymers on plant growth (length, fresh and dry weights of aerial and root parts, number and weight of the obtained fruits). Result: In vitro studies reveal a promoter action of chitosan and the mixture chitin-chitosan on increasing seed germination percentage by 16%, 34% and 22%. Thus, at both 25, 50 and 100 mg/l of chitin or chitosan, increase vigor index, shoot length and root is obtained. In vivo, the effect of chitin and chitosan is limited to stimulation of the lengths, fresh and dry weights of plants. However, a highly significant increase in the number and weight of the fruits is marked by the weekly soil amendment by the chitin-chitosan mixture at 25 mg/l and 100 mg/l.

scholarly journals Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 317 ◽  
Author(s):  
Jitka Viktorova ◽  
Milena Stranska-Zachariasova ◽  
Marie Fenclova ◽  
Libor Vitek ◽  
Jana Hajslova ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Supplements ◽  
Antioxidant Properties ◽  
Absorption Capacity ◽  
In Vitro Assays ◽  
Milk Thistle ◽  
Complex Evaluation

Numerous in vitro assays are used to characterize the antioxidant properties of natural-based matrices. However, many of them generate contradictory and non-compliant results. In our study, we focused on the characterization of traditionally used biochemical (2,2′-azino-bis-(3-ethylbenzothiazoline-6 sulfonic acid) (ABTS), Oxygen Radical Absorption Capacity (ORAC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH)) and cellular (CAA) antioxidant tests on a broad set of milk thistle dietary supplements containing silymarin. In addition to 26 commercially available preparations, also the natural silymarin extract available from Sigma Aldrich, St. Louis, MI, USA, and a model mixture of pure flavonoid/flavonolignans mimicking the silymarin composition were investigated as control samples. Significant differences in the antioxidant capacity of the supplements were observed. Unlike the DPPH, the results of the ABTS and ORAC methods correlated with the silymarin components determined by U-HPLC-HRMS/MS. The responses in CAA were considerably lower than in other assays. Silymarin exhibited a significantly higher antioxidant capacity than the artificially prepared flavonoid/flavonolignans mixture in all tests, indicating possible presence of other antioxidants of natural origin. The follow-up U-HPLC-HRMS/MS screening revealed the presence of tens of non-silymarin compounds with reported antioxidant activity (not only in the silymarin extract, but also in the milk thistle preparations). The sum of the total phenolics and the sum of the simple phenolics correlated with CAA results more than silymarin.

scholarly journals Isolation and structural elucidation of dimeric epigallocatechin-3-gallate autoxidation products and their antioxidant capacity

2021 ◽  
Author(s):  
Julian Alfke ◽  
Uta Kampermann ◽  
Svetlana Kalinina ◽  
Melanie Esselen
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Cd Spectroscopy ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Published Data ◽  
Dietary Polyphenols ◽  
Cellular Effects ◽  
The Impact

AbstractDietary polyphenols like epigallocatechin-3-gallate (EGCG)—which represents the most abundant flavan-3-ol in green tea—are subject of several studies regarding their bioactivity and health-related properties. On many occasions, cell culture or in vitro experiments form the basis of published data. Although the stability of these compounds is observed to be low, many reported effects are directly related to the parent compounds whereas the impact of EGCG degradation and autoxidation products is not yet understood and merely studied. EGCG autoxidation products like its dimers theasinensin A and D, “P2” and oolongtheanin are yet to be characterized in the same extent as their parental polyphenol. However, to investigate the bioactivity of autoxidation products—which would minimize the discrepancy between in vitro and in vivo data—isolation and structure elucidation techniques are urgently needed. In this study, a new protocol to acquire the dimers theasinensin A and D as well as oolongtheanin is depicted, including a variety of spectroscopic and quadrupole time-of-flight high-resolution mass spectrometric (qTOF-HRMS) data to characterize and assign these isolates. Through nuclear magnetic resonance (NMR) spectroscopy, polarimetry, and especially circular dichroism (CD) spectroscopy after enzymatic hydrolysis the complementary atropisomeric stereochemistry of the isolated theasinensins is illuminated and elucidated. Lastly, a direct comparison between the isolated EGCG autoxidation products and the monomer itself is carried out regarding their antioxidant properties featuring Trolox equivalent antioxidant capacity (TEAC) values. These findings help to characterize these products regarding their cellular effects and—which is of special interest in the flavonoid group—their redox properties.

Micropropagation of Pepper (Capsicum annuum L.) Through in vitro Direct Organogenesis Organogenesis

2010 ◽  
Vol 3 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Mahmoud Otroshy ◽  
Kosar Moradi ◽  
Mojtaba Khayam Nekouei ◽  
Paul C. Struik
Keyword(s):  
Capsicum Annuum ◽  
Direct Organogenesis ◽  
Capsicum Annuum L

scholarly journals Susceptibilidad de aislados de Xanthomonas campestris pv. vesicatoria a Streptomyces y extractos bioactivos de Ganoderma

2018 ◽  
Vol 27 (6) ◽  
pp. 30-39
Author(s):  
Loreto Robles-Hernández ◽  
Dámaris Leopoldina Ojeda-Barrios ◽  
Ana Cecilia González-Franco ◽  
Jared Hernández-Huerta ◽  
Nora Aideé Salas-Salazar ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Ganoderma Lucidum ◽  
Xanthomonas Campestris ◽  
Streptomyces Lydicus ◽  
Capsicum Annuum L

En el presente estudio, 10 aislados bacterianos de chile (Capsicum annuum L.) fueron identificados como Xanthomonas campestris pv. vesicatoria; se determinó su patogenicidad en invernadero y se evaluó su susceptibilidad a Streptomyces lydicus y extractos bioactivos de Ganoderma lucidum en condiciones in vitro y de invernadero, utilizando en cada caso un diseño completamente al azar. Todos los aislados causaron infección con una incidencia del 100% y variaciones en su severidad. Aunque todos los aislados fueron susceptibles a los tratamientos biológicos, los extractos bioactivos fueron superiores con un 100% de inhibición in vitro, así como en la reducción de infección foliar y población bacteriana en invernadero. Destacó una correlación fuerte entre población bacteriana e infección foliar (r = 0.9139**) en plantas inoculadas únicamente con el patógeno; en plantas tratadas adicionalmente con los biológicos, solo hubo una correlación débil en cada caso, sugiriendo su efecto benéfico en la supresión de X. campestris pv. vesicatoria.

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