scholarly journals A multi-enzyme cascade reaction for the production of 6-hydroxyhexanoic acid

2019 ◽  
Vol 74 (3-4) ◽  
pp. 71-76 ◽  
Author(s):  
Vishnu S.T. Srinivasamurthy ◽  
Dominique Böttcher ◽  
Uwe T. Bornscheuer
Keyword(s):  
Escherichia Coli ◽  
Cascade Reactions ◽  
Product Inhibition ◽  
Stirred Tank ◽  
Fed Batch ◽  
Batch Mode ◽  
Whole Cells ◽  
Tank Reactor ◽  
Enzyme Cascade

Abstract Multi-enzyme cascade reactions capture the essence of nature’s efficiency by increasing the productivity of a process. Here we describe one such three-enzyme cascade for the synthesis of 6-hydroxyhexanoic acid. Whole cells of Escherichia coli co-expressing an alcohol dehydrogenase and a Baeyer-Villiger monooxygenase (CHMO) for internal cofactor regeneration were used without the supply of external NADPH or NADP+. The product inhibition caused by the ε-caprolactone formed by the CHMO was overcome by the use of lipase CAL-B for in situ conversion into 6-hydroxyhexanoic acid. A stirred tank reactor under fed-batch mode was chosen for efficient catalysis. By using this setup, a product titre of >20 g L−1 was achieved in a 500 mL scale with an isolated yield of 81% 6-hydroxyhexanoic acid.

scholarly journals Recent Advances in Enzymatic and Chemoenzymatic Cascade Processes

Catalysts ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1258
Author(s):  
Noelia Losada-Garcia ◽  
Zaida Cabrera ◽  
Paulina Urrutia ◽  
Carla Garcia-Sanz ◽  
Alicia Andreu ◽  
...  
Keyword(s):  
Organic Chemistry ◽  
Solid Phase ◽  
Cascade Reactions ◽  
Review Article ◽  
The Novel ◽  
Enzyme Cascade ◽  
Cascade Processes ◽  
Novel Strategy ◽  
Substantial Interest

Cascade reactions have been described as efficient and universal tools, and are of substantial interest in synthetic organic chemistry. This review article provides an overview of the novel and recent achievements in enzyme cascade processes catalyzed by multi-enzymatic or chemoenzymatic systems. The examples here selected collect the advances related to the application of the sequential use of enzymes in natural or genetically modified combination; second, the important combination of enzymes and metal complex systems, and finally we described the application of biocatalytic biohybrid systems on in situ catalytic solid-phase as a novel strategy. Examples of efficient and interesting enzymatic catalytic cascade processes in organic chemistry, in the production of important industrial products, such as the designing of novel biosensors or bio-chemocatalytic systems for medicinal chemistry application, are discussed

Batch and Pulsed Fed-Batch Cultures of Aspergillus flavipes FP-500 Growing on Lemon Peel at Stirred Tank Reactor

2015 ◽  
Vol 177 (6) ◽  
pp. 1201-1215 ◽  
Author(s):  
V. E. Wolf-Márquez ◽  
E. García-García ◽  
M. García-Rivero ◽  
G. Aguilar-Osorio ◽  
M. A. Martínez Trujillo
Keyword(s):  
Stirred Tank ◽  
Fed Batch ◽  
Batch Cultures ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Lemon Peel ◽  
Aspergillus Flavipes

scholarly journals A Multi-Enzyme Cascade for the Production of High-Value Aromatic Compounds

Catalysts ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1216
Author(s):  
Claudia Engelmann ◽  
Jens Johannsen ◽  
Thomas Waluga ◽  
Georg Fieg ◽  
Andreas Liese ◽  
...  
Keyword(s):  
Cascade Reactions ◽  
Cofactor Regeneration ◽  
Candida Boidinii ◽  
Phase System ◽  
Second Phase ◽  
Aromatic Ester ◽  
Two Phase ◽  
Enzyme Cascade

Cascade reactions are the basis of life in nature and are adapted to research and industry in an increasing manner. The focus of this study is the production of the high-value aromatic ester cinnamyl cinnamate, which can be applied in flavors and fragrances. A three-enzyme cascade was established to realize the synthesis, starting from the corresponding aldehyde with in situ cofactor regeneration in a two-phase system. After characterization of the enzymes, a screening with different organic solvents was carried out, whereby xylene was found to be the most suitable solvent for the second phase. The reaction stability of the formate dehydrogenase (FDH) from Candida boidinii is the limiting step during cofactor regeneration. However, the applied enzyme cascade showed an overall yield of 54%. After successful application on lab scale, the limitation by the FDH was overcome by immobilization of the enzymes and an optimized downstream process, transferring the cascade into a miniplant. The upscaling resulted in an increased yield for the esterification, as well as overall yields of 37%.

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