scholarly journals A Gain-of-Function Mutation in Synaptotagmin-1 Reveals a Critical Role of Ca2+-Dependent Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor Complex Binding in Synaptic Exocytosis

2006 ◽  
Vol 26 (48) ◽  
pp. 12556-12565 ◽  
Author(s):  
Z. P. Pang ◽  
O.-H. Shin ◽  
A. C. Meyer ◽  
C. Rosenmund ◽  
T. C. Sudhof
Keyword(s):  
Critical Role ◽  
Receptor Complex ◽  
Gain Of Function ◽  
Attachment Protein ◽  
Protein Receptor ◽  
Sensitive Factor ◽  
Synaptotagmin 1 ◽  
Synaptic Exocytosis ◽  
Factor Attachment Protein Receptor

scholarly journals Munc18-1 domain-1 controls vesicle docking and secretion by interacting with syntaxin-1 and chaperoning it to the plasma membrane

2011 ◽  
Vol 22 (21) ◽  
pp. 4134-4149 ◽  
Author(s):  
Gayoung A. Han ◽  
Nancy T. Malintan ◽  
Ner Mu Nar Saw ◽  
Lijun Li ◽  
Liping Han ◽  
...  
Keyword(s):  
Molecular Chaperone ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Attachment Protein ◽  
Vesicle Docking ◽  
Protein Receptor ◽  
Sensitive Factor ◽  
Intracellular Expression ◽  
Factor Attachment Protein Receptor

Munc18-1 plays pleiotropic roles in neurosecretion by acting as 1) a molecular chaperone of syntaxin-1, 2) a mediator of dense-core vesicle docking, and 3) a priming factor for soluble N-ethylmaleimide–sensitive factor attachment protein receptor–mediated membrane fusion. However, how these functions are executed and whether they are correlated remains unclear. Here we analyzed the role of the domain-1 cleft of Munc18-1 by measuring the abilities of various mutants (D34N, D34N/M38V, K46E, E59K, K46E/E59K, K63E, and E66A) to bind and chaperone syntaxin-1 and to restore the docking and secretion of dense-core vesicles in Munc18-1/-2 double-knockdown cells. We identified striking correlations between the abilities of these mutants to bind and chaperone syntaxin-1 with their ability to restore vesicle docking and secretion. These results suggest that the domain-1 cleft of Munc18-1 is essential for binding to syntaxin-1 and thereby critical for its chaperoning, docking, and secretory functions. Our results demonstrate that the effect of the alleged priming mutants (E59K, D34N/M38V) on exocytosis can largely be explained by their reduced syntaxin-1–chaperoning functions. Finally, our data suggest that the intracellular expression and distribution of syntaxin-1 determines the level of dense-core vesicle docking.

Renin release: role of SNAREs

2014 ◽  
Vol 307 (5) ◽  
pp. R484-R486 ◽  
Author(s):  
Mariela Mendez
Keyword(s):  
Molecular Mechanism ◽  
Current Knowledge ◽  
Renin Release ◽  
Juxtaglomerular Cells ◽  
Attachment Protein ◽  
Granule Exocytosis ◽  
Protein Receptor ◽  
Sensitive Factor ◽  
Factor Attachment Protein Receptor

Little is known about the molecular mechanism mediating renin granule exocytosis and the identity of proteins involved. We previously showed that soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNAREs), a family of proteins required for exocytosis, mediate the stimulated release of renin from juxtaglomerular cells. This minireview focuses on the current knowledge of the proteins that facilitate renin-granule exocytosis. We discuss the identity of potential candidates that mediate the signaling and final steps of exocytosis of the renin granule.

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