EFFECTS OF EPINEPHRINE ON THE PRODUCTION AND METABOLIC CLEARANCE OF CORTISOL IN NORMAL MEN AND WOMEN AND IN WOMEN WITH IDIOPATHIC HIRSUTISM

1966 ◽  
Vol 53 (4) ◽  
pp. 561-570 ◽  
Author(s):  
E. J. Segre ◽  
E. H. Friedrich ◽  
O. I. Dodek ◽  
C. W. Lloyd ◽  
J. Lobotsky ◽  
...  
Keyword(s):  
Constant Infusion ◽  
Metabolic Clearance ◽  
Metabolic Clearance Rate ◽  
Men And Women ◽  
Plasma Cortisol Concentration ◽  
Baseline Cortisol ◽  
Idiopathic Hirsutism ◽  
The Mean ◽  
Normal Women ◽  
Normal Men

ABSTRACT The influence of epinephrine on the metabolic clearance rate (MCR) and production rate (PR) of cortisol was determined in normal men and women and in women with idiopathic hirsutism. MCR was measured using a constant infusion of 3H-cortisol and PR calculated as the product of MCR and plasma cortisol concentration according to the model of Tait. After control observations 0.5 μg/kg epinephrine was injected intravenously in one minute followed by a constant infusion at a rate of 0.5 mg/h. The mean cortisol MCR was 10.3 litres/h in males and 7.9 litres/h in females. In neither sex was it altered by epinephrine. The mean baseline cortisol PR in males was 568 μg/h; it increased to 940 μg/h with epinephrine (P < .025 > .01). In hirsute females the mean baseline cortisol PR was 697 μg/h with an increase to 1067 μg/h with epinephrine. In normal females, the increase in cortisol PR was less (515 μg/h to 638 μg/h). Adrenal responsiveness to epinephrine, as measured by the production of cortisol, appears greater in hirsute than in normal women. Possible relationships between this difference and the development of hirsutism are discussed.

INFUSIONS OF hFSH AND hLH IN NORMAL MEN

1976 ◽  
Vol 81 (1) ◽  
pp. 225-233 ◽  
Author(s):  
J. M. Kjeld ◽  
P. Harsoulis ◽  
S. F. Kuku ◽  
J. C. Marshall ◽  
B. Kaufman ◽  
...  
Keyword(s):  
Slow Component ◽  
Follicle Stimulating Hormone ◽  
Metabolic Clearance ◽  
Metabolic Clearance Rate ◽  
Average Value ◽  
Endogenous Production ◽  
Serial Serum ◽  
The Mean ◽  
Distribution Spaces ◽  
Normal Men

ABSTRACT Serial serum assays of immunoreactive FSH before, during and after a 4 h intravenous infusion of human follicle stimulating hormone (hFSH) in five healthy men revealed two disappearance rate constants with corresponding mean half-lives of 2.9 and 50.6 h. The mean distribution spaces calculated for the fast and slow component were 4.36 1 and 75.9 1 respectively. The average value for the metabolic clearance rate was 17.2 ml/min and for the endogenous production rate 50.9 mU/min. Two of the subjects had human luteinizing hormone (hLH) added in the infusions without the FSH kinetics changing.

ANDROGEN PRODUCTION AND SKIN METABOLISM IN HIRSUTISM

1977 ◽  
Vol 75 (1) ◽  
pp. 83-91 ◽  
Author(s):  
FRÉDÉRIQUE KUTTENN ◽  
IRÉNE MOWSZOWICZ ◽  
GILBERT SCHAISON ◽  
PIERRE MAUVAIS-JARVIS
Keyword(s):  
High Rate ◽  
Testosterone Metabolism ◽  
Men And Women ◽  
Skin Metabolism ◽  
Idiopathic Hirsutism ◽  
Sexual Skin ◽  
Normal Women ◽  
Normal Men ◽  
Increased Activity

The concentrations of testosterone, androstenedione and dihydrotestosterone (DHT) in the plasma and 5α-androstane-3α,17β-diol (androstanediol) in the urine were measured in 40 women with hirsutism of ovarian, adrenal and idiopathic origin. Conversion of [3H]testosterone to DHT, 3α- and 3β-androstanediols was also studied in homogenates of pubic skin obtained from 15 of the patients. Results were compared with values obtained from normal men and women. Values for the levels of testosterone, DHT and androstenedione in the plasma and androstanediol in the urine of hirsute women were all above control levels, especially for plasma androstenedione and urinary androstanediol (P < 0·001). This finding was particularly marked in patients with hirsutism of ovarian origin. Conversion of [3H]testosterone to 5α-reduced metabolites by homogenates of skin obtained from hirsute women was significantly greater than by homogenates of skin from normal women (P < 0·001) but was the same as the value for normal men. The highest values for conversion were obtained from the patients with idiopathic hirsutism. These results indicate that androstenedione is the principal androgen secreted in hirsutism. In sexual skin this steroid may be converted to DHT and 3α-, and 3β-androstanediols and the increased activity of testosterone 5α-reductase may result in an exaggerated 'utilization' of androstenedione in this tissue. The high rate of excretion of androstanediol in the urine of patients with idiopathic hirsutism may be explained by the fact that this steroid is an end-product of testosterone metabolism.

scholarly journals Testosterone Metabolic Clearance and Production Rates Determined by Stable Isotope Dilution/Tandem Mass Spectrometry in Normal Men: Influence of Ethnicity and Age

2004 ◽  
Vol 89 (6) ◽  
pp. 2936-2941 ◽  
Author(s):  
Christina Wang ◽  
Don H. Catlin ◽  
Borislav Starcevic ◽  
Andrew Leung ◽  
Emma DiStefano ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
White Men ◽  
Constant Infusion ◽  
Metabolic Clearance ◽  
Tandem Mass ◽  
Stable Isotope Dilution ◽  
The Mean ◽  
Normal Men

Abstract The metabolic clearance rate (MCRT) and production rate (PRT) of testosterone (T) were measured using constant infusion of trideuterated (d3) T and quantitating serum d3T by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Serum unlabeled T (d0T) was measured by LC-MS-MS, and serum total T (d3T + d0T) was measured by RIA. Mean MCRT (measured by LC-MS-MS) in young white men (1272 ± 168 liters/d) was not significantly different from young Asian men (1070 ± 166 liters/d). Mean PRT was also not significantly different between the two ethnic groups (whites, 9.11 ± 1.11 mg/d; Asians, 7.22 ± 1.15 mg/d; P = 0.19 using d0T data). Both the mean MCRT (812 ± 64 liters/d; P &lt; 0.01) and the PRT (3.88 ± 0.27 mg/d; P &lt; 0.001) were significantly lower in middle-aged white men when compared with their younger counterparts. The mean MCRT and PRT calculated using serum total T or d0T data showed a diurnal variation, with levels at midday significantly higher than those measured in the evening in the young (MCRT, P &lt; 0.01; PRT, P &lt; 0.001) and to a lesser extent in the older men (MCRT, P &lt; 0.05; PRT, P &lt; 0.05 using total T and P &lt; 0.001 using d0T data). We conclude that using LC-MS-MS to detect d3T in serum after constant infusion of stable isotope-labeled T allows the measurements of MCRT and PRT, which can be used to study androgen metabolism repeatedly after physiological or pharmacological interventions.

THE EFFECT OF EPINEPHRINE ON TESTOSTERONE PRODUCTION

1967 ◽  
Vol 55 (1) ◽  
pp. 184-192 ◽  
Author(s):  
Joseph Levin ◽  
Charles W. Lloyd ◽  
Julia Lobotsky ◽  
E. H. Friedrich
Keyword(s):  
Production Rate ◽  
Mean Value ◽  
Constant Infusion ◽  
Plasma Testosterone ◽  
Metabolic Clearance ◽  
Metabolic Clearance Rate ◽  
Testosterone Concentration ◽  
The Mean ◽  
Male Subjects ◽  
Double Isotope

ABSTRACT The metabolic clearance rate (MCR) and the production rate (PR) of testosterone were measured in four male subjects by the method of constant infusion of tritiated testosterone. The mean value of the MCR of 1161 ± 80 (SD) liters/24 hours was not altered by the infusion of epinephrine, at the rate of 0.466 mg per hour for three: hours. The plasma testosterone concentration was measured by the double isotope method of Riondel et al. (1963). Epinephrine significantly decreased this concentration (28%) and also the production rate (28%) The effect of epinephrine on plasma testosterone concentration was measured in six additional male subjects, and the results of the total of 10 subjects showed that there was a decrease of (28%) in the concentration. It was concluded that epinephrine significantly diminished the production rate of testosterone.

CRITICAL ANALYSIS OF METHODS FOR ESTIMATING PRODUCTION RATES OF FSH

1975 ◽  
Vol 80 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Salvatore Raiti ◽  
Robert M. Blizzard ◽  
Robert Penny ◽  
Claude J. Migeon
Keyword(s):  
Urinary Excretion ◽  
Clearance Rate ◽  
Critical Analysis ◽  
Constant Infusion ◽  
Metabolic Clearance ◽  
Production Rates ◽  
Metabolic Clearance Rate ◽  
Normal Men ◽  
Infusion Technique ◽  
Normal Adults

ABSTRACT The urinary excretion method and the constant infusion technique were used to estimate hFSH production rates (PR) in three normal men (aged 25–40 years). Both studies were also done in a hypophysectomized nine year old boy. The PR of hFSH in the urinary excretion studies ranged from 20.0 to 45.7 IU/24 h, whereas the constant infusion studies yielded PR's of 68.7 to 107.8 IU/24 h. Possible reasons for the discrepancy of the results obtained by the two methods are discussed. In the hypophysectomized patient, the metabolic clearance rate (MCR) of infused hFSH was much lower (4.3 ml/min) than in the normal adults (7.8 to 12.0 ml/min) and was not affected by the dose of hFSH given.

Hysterical traits and variability of mood in normal men

1979 ◽  
Vol 9 (2) ◽  
pp. 301-304 ◽  
Author(s):  
Peter V. Rabins ◽  
Phillip R. Slavney
Keyword(s):  
Men And Women ◽  
Normal Women ◽  
Normal Men

SynopsisIn a study of 40 normal men it was found that self-ratings on variability of mood were positively correlated with self-ratings on hysterical traits. These results are similar to those found in normal women and lend support both to the validity of the concept of hysterical personality and to the idea that men and women experience fluctuations of mood in a similar way.

Glucose and lactate kinetics after endotoxin administration in dogs.

1977 ◽  
Vol 232 (2) ◽  
pp. E180 ◽  
Author(s):  
R R Wolfe ◽  
D Elahi ◽  
J J Spitzer
Keyword(s):  
Lactate Concentration ◽  
Constant Infusion ◽  
Glucose Turnover ◽  
Metabolic Clearance ◽  
Plasma Lactate ◽  
Metabolic Clearance Rate ◽  
Metabolic Substrate ◽  
E Coli ◽  
Lactate Kinetics ◽  
Plasma Lactate Concentration

We studied the effects of E. coli endotoxin on the glucose and lactate kinetics in dogs by means of the primed constant infusion of [6(-3)H] glucose and Na-L-(+)-[U-14C] lactate. The infusion of endotoxin induced a transient hyperglycemic level, followed by a steady fall in plasma glucose to hypoglycemic levels. The rate of appearance (Ra) and the rate of disappearance (Rd) of glucose were both significantly elevated (P less than .05) for 150 min after endotoxin, after which neither differed from the preinfusion value. The metabolic clearance rate of glucose was significantly elevated at all times 30 min postendotoxin. By 30 min postendotoxin, Ra and Rd of lactate, plasma lactate concentration, and the percent of glucose turnover originating from lactate were significantly elevated and remained so for the duration of the experiment. We concluded that after endotoxin hypoglycemia developed because of an enhanced peripheral uptake of glucose and a failure of the liver to maintain an increased Ra of glucose. We also concluded that lactate became an important precursor for gluconeogenesis and an important metabolic substrate.

Distribution volume, metabolic clearance and plasma half disappearance time of exogenous luteinizing hormone releasing hormone in normal women and women with obesity and anorexia nervosa

1981 ◽  
Vol 96 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Kazumasa Chikamori ◽  
Fumie Suehiro ◽  
Toshiki Ogawa ◽  
Kei Sato ◽  
Hiroyoshi Mori ◽  
...  
Keyword(s):  
Body Weight ◽  
Anorexia Nervosa ◽  
Normal Subjects ◽  
Distribution Volume ◽  
Metabolic Clearance ◽  
Obese Patients ◽  
Metabolic Clearance Rate ◽  
Provocation Tests ◽  
Disappearance Time ◽  
Normal Women

Abstract. Synthetic LRH was infused into normal women and women with obesity and anorexia nervosa to determine the distribution volume (DV), metabolic clearance rate (MCR) and half disappearance time (t½) of plasma LRH. In normal women, the DV of LRH was 12.1 ± 0.9 (mean ± se) l, the MCR was 1478.9 ± 39.8 ml/min (28.5 ± 1.2 ml/min/kg body weight) and the initial t½ was 5.6 ± 0.4 min. In obese patients the DV (20.6 ± 1.5 l) was significantly higher than that in normal subjects (P < 0.005), but the MCR and t½ were not significantly different from those in normal subjects. In patients with anorexia nervosa the DV and MCR were 6.5 ± 1.1 l and 621.8 ± 110.5 ml/min (17.9 ± 2.4 ml/ min/kg body weight), respectively, which were both significantly lower than those in normal subjects (P < 0.02), while the t½ (7.3 ± 0.1 min) was longer than in normal subjects (P < 0.02). These data suggest that 1) the abnormal responses of some hormones to provocation tests observed in obese patients and patients with anorexia nervosa should be evaluated in consideration of changes in the DV and metabolic clearance of hormones in these conditions, and 2) in patients with anorexia nervosa changes in MCR and t½ may reflect low metabolism of LRH.

Production rate, metabolic clearance rate and blood concentration of progesterone in conscious and anaesthetized pregnant rats

1984 ◽  
Vol 102 (3) ◽  
pp. 357-363 ◽  
Author(s):  
B. J. Waddell ◽  
N. W. Bruce
Keyword(s):  
Production Rate ◽  
Clearance Rate ◽  
Blood Concentration ◽  
Constant Infusion ◽  
Metabolic Clearance ◽  
Metabolic Clearance Rate ◽  
Short Term ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
Anaesthetized Rats

ABSTRACT Both production rate and metabolic clearance rate (MCR) of progesterone may vary rapidly and so effect short-term changes in blood concentration of the hormone. Here, a constant infusion and sampling technique was used to estimate these three characteristics of progesterone metabolism in seven conscious and ten anaesthetized rats on day 16 of pregnancy. After steady state was achieved, four samples were collected during a 1-h period from each rat. Mean values for production rate and MCR of progesterone in conscious rats were 14·0 ±1·4 μmol/day and 63·2 ± 6·2 litres/day respectively. Both values were substantially reduced in anaesthetized rats (8.6 ±0·8 μmol/ day and 39·4± 3·4 litres/day respectively) and so blood concentration was unchanged. The production rate was positively related to the total mass of luteal tissue (common correlation coefficient, r = 0·61, P <0·05). There were no consistent changes in the three characteristics with time but variation within rats was high. The estimated coefficients of variation for production rate, MCR and blood concentration within rats were 26, 18 and 17% in conscious and 27, 20 and 23% in anaesthetized rats respectively. Short-term changes in production rate and MCR generally were in the same direction (P <0·05). This reduced variation in blood concentration which would otherwise have occurred if production rate and MCR were unrelated. The pregnant rat is clearly capable of rapid shifts in production rate, MCR and blood concentration of progesterone and the positive relationship between production rate and MCR has a homeostatic effect on blood concentration. J. Endocr. (1984) 102, 357–363

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