Serum levels of testosterone, cortisol, prolactin and bioactive luteinizing hormone in adult male rhesus monkeys following cage-restraint or anaesthetizing with ketamine hydrochloride

1981 ◽  
Vol 97 (1) ◽  
pp. 118-124 ◽  
Author(s):  
C. P. Puri ◽  
V. Puri ◽  
T. C. Anand Kumar
Keyword(s):  
Luteinizing Hormone ◽  
Adult Male ◽  
Rhesus Monkeys ◽  
Serum Levels ◽  
Acute Effects ◽  
Experimental Procedure ◽  
Blood Samples ◽  
Ketamine Hydrochloride ◽  
Circulating Levels ◽  
Male Rhesus

Abstract. The present studies were carried out to characterize and compare the acute effects of cage-restraint or administration of a mild anaesthetic on serum levels of testosterone (T) cortisol (C), prolactin (Prl) and bioactive luteinizing hormone (LH) in adult male rhesus monkeys. Serum T levels declined progressively while C levels increased in the 3 sequential blood samples obtained at 20 min intervals from restrained monkeys. Prl and LH levels in the serum remained unaltered. Following single or multiple injections of ketamine hydrochloride (10 mg/kg) serum T levels did not decline progressively as seen in the blood samples obtained from the cage-restrained, conscious monkeys. Serum C and Prl levels showed a progressive rise following anaesthetization. LH levels were not affected by the anaesthetic. The findings reported here are of pertinent relevance to the evaluation of acute effects of any experimental procedure aimed at altering circulating levels of the hormones studied.

Pituitary response to LRH and TRH stimulation and peripheral steroid hormones in conscious and anaesthetized adult male rhesus monkeys (Macaca mulatta)

1980 ◽  
Vol 93 (3) ◽  
pp. 287-293 ◽  
Author(s):  
E. Jean Wickings ◽  
E. Nieschlag
Keyword(s):  
Adult Male ◽  
Rhesus Monkeys ◽  
Serum Testosterone ◽  
Fold Increase ◽  
Serum Cortisol ◽  
Serum Levels ◽  
Biologically Active ◽  
Similar Response ◽  
Response Curves ◽  
Male Rhesus

Abstract. Adult male rhesus monkeys are aggressive animals and very difficult to handle. Hence experimental manipulations necessarily involve the use of restraint procedures, either chemical or physical, which may influence endocrine functions. Therefore, the effects of ketamine anaesthesia on basal hormone levels and on the pituitary response to LRH and TRH were investigated in 4 adult male rhesus monkeys. Values were compared to those obtained from the same animals restrained in primate chairs for approximately 48 h, a procedure to which they had been accustomed to over the preceding 6 months. Serum cortisol levels under anaesthesia were at all times lower than in conscious monkeys, but increased after 2 h to values twice as high as measured initially. Serum testosterone concentrations were not significantly different on the two occasions, but levels under anaesthesia were slightly higher initially than in the conscious monkeys, and decreased gradually over the 3 h test period. Initial prolactin levels were lower in the anaesthetized monkeys, and increased 2–3-fold after 90 min; values at 3 h were not significantly different from those in conscious monkeys. Intravenous TRH elicited a similar response in prolactin on both occasions, maximum values occurring after 15–30 min and returning to basal levels after 3 h. The maximum values attained and the area under the response curves were higher under anaesthesia. LRH stimulation resulted in a 15- and 30-fold increase in serum levels of biologically active LH, with and without anaesthesia, respectively. Basal levels were not significantly different on the two occasions. The area under the LH response curve was higher in 3 of the 4 monkeys without anaesthesia. The extent to which results in conscious monkeys are affected by stress is difficult to assess. Since neither handling technique allows for the collection of 'true' basal data, it is paramount to standardize and define the conditions under which experiments, and even routine blood sampling, are performed in male rhesus monkeys.

Inhibition of Aromatization Stimulates Luteinizing Hormone and Testosterone Secretion in Adult Male Rhesus Monkeys*

1984 ◽  
Vol 59 (6) ◽  
pp. 1088-1096 ◽  
Author(s):  
WILLIAM E. ELLINWOOD ◽  
DAVID L. HESS ◽  
CHARLES E. ROSELLI ◽  
HAROLD G. SPIES ◽  
JOHN A. RESKO
Keyword(s):  
Luteinizing Hormone ◽  
Adult Male ◽  
Rhesus Monkeys ◽  
Testosterone Secretion ◽  
Male Rhesus

scholarly journals The mechanism of end-organ resistance to 1α,25-dihydroxycholecalciferol in the common marmoset

1985 ◽  
Vol 227 (2) ◽  
pp. 555-563 ◽  
Author(s):  
N Takahashi ◽  
S Suda ◽  
T Shinki ◽  
N Horiuchi ◽  
Y Shiina ◽  
...  
Keyword(s):  
Rhesus Monkeys ◽  
World Monkey ◽  
Daily Intake ◽  
Normal Growth ◽  
Common Marmoset ◽  
Serum Levels ◽  
Normal Diet ◽  
The Common ◽  
Diet Intake ◽  
Circulating Levels

The common marmoset, a New World monkey, requires a large amount of cholecalciferol (110 i.u./day per 100g body wt.) to maintain its normal growth. In a previous report, we demonstrated that the circulating levels of 1 alpha, 25-dihydroxycholecalciferol [1 alpha,25(OH)2D3] in the marmosets are much higher than those in rhesus monkeys and humans, but the marmosets are not hypercalcaemic [Shinki, Shiina, Takahashi, Tanioka, Koizumi & Suda (1983) Biochem. Biophys. Res. Commun. 14, 452-457]. To compare the effect of the daily intake of cholecalciferol, two rhesus monkeys were given a large amount of cholecalciferol (900 i.u./day per 100g body wt). Their serum levels of calcium, 25-hydroxycholecalciferol and 24R,25-dihydroxycholecalciferol were markedly elevated, but the serum 1 alpha,25(OH)2D3 levels remained within a range similar to those in the rhesus monkeys fed the normal diet (intake of cholecalciferol 5 i.u./day per 100g body wt). Intestinal cytosols prepared from both monkeys contained similar 3.5 S macromolecules to which 1 alpha,25(OH)2D3 was bound specifically. However, the cytosols from the marmosets contained only one-sixth as many 1 alpha,25(OH)2D3 receptors as those from the rhesus monkeys. Furthermore, the activity of the 1 alpha,25(OH)2D3-receptor complex in binding to DNA-cellulose was very low in the marmosets. These results suggest that the marmoset possesses an end-organ resistance to 1 alpha,25(OH)2D3 and is a useful animal model for studying the mechanism of vitamin D-dependent rickets, type II.

Effects of testosterone on cognition in young adult male rhesus monkeys

2009 ◽  
Vol 98 (5) ◽  
pp. 524-531 ◽  
Author(s):  
Agnès Lacreuse ◽  
Margaret R. Chiavetta ◽  
Ashley-Ann C. Shirai ◽  
Jerrold S. Meyer ◽  
Daniel R. Grow
Keyword(s):  
Young Adult ◽  
Adult Male ◽  
Rhesus Monkeys ◽  
Young Adult Male ◽  
Male Rhesus
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