SETDB1 plays an essential role in maintenance of gonocyte survival in pigs

Histone methyltransferase SETDB1 suppresses gene expression and modulates heterochromatin formation through H3K9me2/3. Previous studies have revealed that SETDB1 catalyzes lysine 9 of histone H3 tri-methylation and plays essential roles in maintaining the survival of embryonic stem cells and spermatogonial stem cells in mice. However, the function of SETDB1 in porcine male germ cells remains unclear. The aim of the present study was to reveal the expression profile and function of SETDB1 in porcine germ cells. SETDB1 expression gradually increased during testis development. SETDB1 was strongly localized in gonocytes. Knockdown of SETDB1 gene expression led to gonocyte apoptosis and a decrease in H3K27me3, but no significant change in H3K9me3. These observations suggested that SETDB1 is a novel epigenetic regulator of porcine male germ cells, and contributes to the maintenance of gonocyte survival in pigs, probably due to the regulation of H3K27me3 rather than H3K9me3. These findings will provide a theoretical basis for the future study of epigenetic regulation of spermatogenesis.

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THE USE OF SEMIPERMEABLE SPHERES ENGULFED WITH MOUSE EMBRYONIC STEM CELLS TO GENERATE MALE GERM CELLS

Fertility and Sterility ◽

10.1016/j.fertnstert.2020.08.1273 ◽

2020 ◽

Vol 114 (3) ◽

pp. e439

Author(s):

Sherina Lawrence ◽

Mounia Haddad ◽

Philip Xie ◽

Zev Rosenwaks ◽

Gianpiero D. Palermo

Keyword(s):

Stem Cells ◽

Embryonic Stem Cells ◽

Germ Cells ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Male Germ Cells

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MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans

Stem Cells International ◽

10.1155/2016/3984937 ◽

2016 ◽

Vol 2016 ◽

pp. 1-17 ◽

Cited By ~ 14

Author(s):

Irma Virant-Klun ◽

Anders Ståhlberg ◽

Mikael Kubista ◽

Thomas Skutella

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Embryonic Stem Cells ◽

Germ Cells ◽

Noncoding Rna ◽

Ovarian Function ◽

Embryonic Stem ◽

Cell Communication ◽

Female Fertility ◽

Control Of Gene Expression

MicroRNAs are a family of naturally occurring small noncoding RNA molecules that play an important regulatory role in gene expression. They are suggested to regulate a large proportion of protein encoding genes by mediating the translational suppression and posttranscriptional control of gene expression. Recent findings show that microRNAs are emerging as important regulators of cellular differentiation and dedifferentiation, and are deeply involved in developmental processes including human preimplantation development. They keep a balance between pluripotency and differentiation in the embryo and embryonic stem cells. Moreover, it became evident that dysregulation of microRNA expression may play a fundamental role in progression and dissemination of different cancers including ovarian cancer. The interest is still increased by the discovery of exosomes, that is, cell-derived vesicles, which can carry different proteins but also microRNAs between different cells and are involved in cell-to-cell communication. MicroRNAs, together with exosomes, have a great potential to be used for prognosis, therapy, and biomarkers of different diseases including infertility. The aim of this review paper is to summarize the existent knowledge on microRNAs related to female fertility and cancer: from primordial germ cells and ovarian function, germinal stem cells, oocytes, and embryos to embryonic stem cells.

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Nanos2 promotes differentiation of chicken (Gallus gallus ) embryonic stem cells to male germ cells

Journal of Cellular Biochemistry ◽

10.1002/jcb.26528 ◽

2018 ◽

Vol 119 (6) ◽

pp. 4435-4446 ◽

Cited By ~ 1

Author(s):

Wenhui Zhang ◽

Yulin Bi ◽

Yingjie Wang ◽

Dong Li ◽

Nana He ◽

...

Keyword(s):

Stem Cells ◽

Embryonic Stem Cells ◽

Germ Cells ◽

Embryonic Stem ◽

Gallus Gallus ◽

Male Germ Cells

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Effect of Zinc Ions on Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ Cells and Some Germ Cell-Specific Gene Expression in Rams

Biological Trace Element Research ◽

10.1007/s12011-012-9484-8 ◽

2012 ◽

Vol 150 (1-3) ◽

pp. 137-146 ◽

Cited By ~ 19

Author(s):

Mohammad Ghasemzadeh-Hasankolai ◽

Roozali Batavani ◽

Mohamadreza Baghaban Eslaminejad ◽

Mohammadali Sedighi-Gilani

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Germ Cell ◽

Germ Cells ◽

Specific Gene ◽

Zinc Ions ◽

Male Germ Cells ◽

Specific Gene Expression

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Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice

genesis ◽

10.1002/gene.20153 ◽

2005 ◽

Vol 43 (1) ◽

pp. 34-42 ◽

Cited By ~ 5

Author(s):

Eiji Mizutani ◽

Hiroshi Ohta ◽

Satoshi Kishigami ◽

Nguyen Van Thuan ◽

Takafusa Hikichi ◽

...

Keyword(s):

Stem Cells ◽

Embryonic Stem Cells ◽

Germ Cells ◽

Embryonic Stem ◽

Chimeric Mice ◽

Male Germ Cells

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The TAZ2 domain of CBP/p300 directs acetylation towards H3K27 within chromatin

10.1101/2020.07.21.214338 ◽

2020 ◽

Author(s):

Thomas W. Sheahan ◽

Viktoria Major ◽

Kimberly M. Webb ◽

Elana Bryan ◽

Philipp Voigt

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Enzymatic Activity ◽

Crucial Role ◽

Regulation Of Gene Expression ◽

Histone H3 ◽

Embryonic Stem ◽

Site Specific ◽

Lysine Residues

AbstractThe closely related acetyltransferases CBP and p300 are key regulators of gene expression in metazoans. CBP/p300 acetylate several specific lysine residues within nucleosomes, including histone H3 lysine 27 (H3K27), a hallmark of active enhancers and promoters. However, it has remained largely unclear how specificity of CBP/p300 towards H3K27 is achieved. Here we show that the TAZ2 domain of CBP is required for efficient acetylation of H3K27, while curbing activity towards other lysine residues within nucleosomes. We find that TAZ2 is a sequence-independent DNA binding module, promoting interaction between CBP and nucleosomes, thereby enhancing enzymatic activity and regulating substrate specificity of CBP. TAZ2 is further required to stabilize CBP binding to chromatin in mouse embryonic stem cells, facilitating specificity towards H3K27 and modulating gene expression. These findings reveal a crucial role of TAZ2 in regulating H3K27ac, while highlighting the importance of correct site-specific acetylation for proper regulation of gene expression.

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Nucleosomal Asymmetry Shapes Histone Mark Binding and Promotes Poising at Bivalent Domains

10.1101/2021.02.08.430127 ◽

2021 ◽

Author(s):

Elana Bryan ◽

Marie Warburton ◽

Kimberly M. Webb ◽

Katy A. McLaughlin ◽

Christos Spanos ◽

...

Keyword(s):

Stem Cells ◽

Embryonic Stem Cells ◽

Neuronal Differentiation ◽

Histone Acetyltransferase ◽

Histone H3 ◽

Embryonic Stem ◽

Histone Mark ◽

Developmental Genes ◽

Chromatin Proteins ◽

And Function

SummaryPromoters of developmental genes in embryonic stem cells (ESCs) are marked by histone H3 lysine 4 trimethylation (H3K4me3) and H3K27me3 in an asymmetric nucleosomal conformation, with each sister histone H3 carrying only one mark. These bivalent domains are thought to poise genes for timely activation upon differentiation. Here we show that asymmetric bivalent nucleosomes recruit repressive H3K27me3 binders but fail to enrich activating H3K4me3 binders, despite presence of H3K4me3, thereby promoting a poised state. Strikingly, the bivalent mark combination further attracts chromatin proteins that are not recruited by each mark individually, including the histone acetyltransferase complex KAT6B (MORF). Knockout of KAT6B blocks neuronal differentiation, demonstrating that bivalency-specific readers are critical for proper ESC differentiation. These findings reveal how histone mark bivalency directly promotes establishment of a poised state at developmental genes, while highlighting how nucleosomal asymmetry is critical for histone mark readout and function.

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