scholarly journals The pathophysiological changes associated with neonatal death of cloned pigs

Reproduction ◽  
2020 ◽  
Vol 160 (2) ◽  
pp. 193-203
Author(s):  
Zheng Ao ◽  
Ting Gu ◽  
Huaxing Zhao ◽  
Junsong Shi ◽  
Enqin Zheng ◽  
...  
Keyword(s):  
Neonatal Death ◽  
Life Sciences ◽  
Purine Metabolism ◽  
Neonatal Mortality Rate ◽  
Metabolism Regulation ◽  
Renal Morphology ◽  
Kidney Morphology ◽  
Purine Metabolite ◽  
And Function ◽  
Very High

Cloned pigs generated by the somatic cell transfer nuclear (SCNT) technique are highly valuable for agriculture, biomedicine, and life sciences. However, the neonatal mortality rate of cloned pigs is very high. The reasons causing the massive loss of cloned pigs during their neonatal ages are unclear. In the present study, we found that the neonatal death of cloned pigs was associated with aberrant purine metabolism, impaired renal morphology and function, and decreased hepatic Hprt1 expression. The downregulation of Hprt1, a key purine metabolism regulation gene, in the liver was responsible for the elevation of an important purine metabolite, uric acid, in the serum, causing abnormalities in kidney morphology and function and leading to death of neonatal cloned pigs. This study provided insights into the pathophysiological mechanisms underlying the neonatal death of clone pigs, and results will help improve their survival rate.

Subunit δ of Chloroplast F0F1 ATPase and OSCP of Mitochondrial F0F1 ATPase: a Comparison by CD-Spectroscopy

1991 ◽  
Vol 46 (9-10) ◽  
pp. 759-764 ◽  
Author(s):  
Siegfried Engelbrecht ◽  
Jennifer Reed ◽  
François Penin ◽  
Danièle C. Gautheron ◽  
Wolfgang Junge
Keyword(s):  
Primary Structure ◽  
Tertiary Structure ◽  
Atp Synthesis ◽  
Structural Similarity ◽  
Cd Spectroscopy ◽  
Α Helix ◽  
And Function ◽  
High Degree ◽  
Very High ◽  
Secondary And Tertiary Structure

Abstract CD spectra have been recorded with subunit δ from chloroplast CF0CF1 and with OSCP from mitochondrial MF0MF1. These subunits are supposed to act similarly at the interface between proton transport through the F0-portion and ATP-synthesis in the F1-portion of their respective F0F1-ATPase. Evaluation of the data for both proteins revealed a very high α-helix content of -85% and practically no β-sheets. Despite their low homology on the primary structure level (23% identity) and their different electrostatic properties (pl-values differ by 3 units), spinach δ and porcine OSCP are indistinguishable with respect to their secondary structure as measured by CD. Prediction and analysis of consensual a-helices even in poorly conserved regions indicate α high degree of structural similarity between chloroplast δ and OSCP. In view of the topology and function of δ and OSCP in intact F0F1 these findings are interpreted to indicate the dominance of secondary and tertiary structure over the primary structure in their supposed function between proton flow and ATP-synthesis.

Long-Term Follow-Up of Renal Morphology and Function in Children after Lithotripsy

1995 ◽  
Vol 54 (2) ◽  
pp. 95-98 ◽  
Author(s):  
Kemal Sanca ◽  
Sadettin Küpei ◽  
Nese Sarica ◽  
Orhan Gögüş ◽  
Sahir Kihç ◽  
...  
Keyword(s):  
Renal Morphology ◽  
Long Term Follow Up ◽  
And Function

scholarly journals Visualization of a Cytoskeleton-like Ftsz Network in Chloroplasts

2000 ◽  
Vol 151 (4) ◽  
pp. 945-950 ◽  
Author(s):  
Justine Kiessling ◽  
Sven Kruse ◽  
Stefan A. Rensing ◽  
Klaus Harter ◽  
Eva L. Decker ◽  
...  
Keyword(s):  
Life Sciences ◽  
Dependent Manner ◽  
Bacterial Cell Division ◽  
Subcellular Structure ◽  
Form And Function ◽  
Intron Structure ◽  
Division Process ◽  
Eukaryotic Organisms ◽  
And Function ◽  
Dose Dependent

It has been a long-standing dogma in life sciences that only eukaryotic organisms possess a cytoskeleton. Recently, this belief was questioned by the finding that the bacterial cell division protein FtsZ resembles tubulin in sequence and structure and, thus, may be the progenitor of this major eukaryotic cytoskeletal element. Here, we report two nuclear-encoded plant ftsZ genes which are highly conserved in coding sequence and intron structure. Both their encoded proteins are imported into plastids and there, like in bacteria, they act on the division process in a dose-dependent manner. Whereas in bacteria FtsZ only transiently polymerizes to a ring-like structure, in chloroplasts we identified persistent, highly organized filamentous scaffolds that are most likely involved in the maintenance of plastid integrity and in plastid division. As these networks resemble the eukaryotic cytoskeleton in form and function, we suggest the term “plastoskeleton” for this newly described subcellular structure.

Critical Prospective Appraisal of Renal Morphology and Function in Children Undergoing Shockwave Lithotripsy and Percutaneous Nephrolithotomy

2007 ◽  
Vol 21 (9) ◽  
pp. 961-966 ◽  
Author(s):  
Pankaj Wadhwa ◽  
Monish Aron ◽  
Chandra Sekhar Bal ◽  
B. Dhanpatty ◽  
Narmada P. Gupta
Keyword(s):  
Percutaneous Nephrolithotomy ◽  
Shockwave Lithotripsy ◽  
Renal Morphology ◽  
And Function

Fgl2 deficiency causes neonatal death and cardiac dysfunction during embryonic and postnatal development in mice

2007 ◽  
Vol 31 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Junwu Mu ◽  
Dawei Qu ◽  
Agata Bartczak ◽  
M. James Phillips ◽  
Justin Manuel ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Cardiac Dysfunction ◽  
Neonatal Death ◽  
Posterior Wall ◽  
Ultrasound Biomicroscopy ◽  
Left Ventricular ◽  
Mode Analysis ◽  
Posterior Wall Thickness ◽  
Perinatal Lethality ◽  
And Function

We hypothesized that cardiac dysfunction was responsible for the high perinatal lethality that we previously reported in fibrinogen-like protein 2 ( Fgl2) knockout (KO) mice. We therefore used ultrasound biomicroscopy to assess left ventricular (LV) cardiac structure and function during development in Fgl2 KO and wild-type (WT) mice. The only deaths observed between embryonic day (E)8.5 (onset of heart beating) and postnatal day (P)28 (weaning) were within 3 days after birth, when 33% of Fgl2 KO pups died. Histopathology and Doppler assessments suggested that death was due to acute congestive cardiac failure without evidence of valvular or other obvious cardiac structural abnormalities. Heart rates in Fgl2 KO embryos were significantly reduced at E8.5 and E17.5, and irregular heart rhythms were significantly more common in Fgl2 KO (21/26) than WT (2/21) embryos at E13.5. Indexes of systolic and/or diastolic cardiac function were also abnormal in KO mice at E13.5 and E17.5, in postnatal mice studied at P1, and in KO mice surviving to P28. M-mode analysis showed no difference in LV diastolic chamber dimension, although posterior wall thickness was thinner at P7 and P28 in Fgl2 KO mice. We conclude that Fgl2 deficiency is not associated with obvious structural cardiac defects but is associated with a high incidence of neonatal death as well as contractile dysfunction and rhythm abnormalities during embryonic and postnatal development in mice.

Research on Application of WMSNs in Ubiquitous Learning Environment

2013 ◽  
Vol 659 ◽  
pp. 229-232 ◽  
Author(s):  
Jin Hua Chen ◽  
Jing Song ◽  
Ying Li
Keyword(s):  
Learning Environment ◽  
Multimedia Data ◽  
Sensor Technology ◽  
Ubiquitous Learning ◽  
Multimedia Data Transmission ◽  
Research On Application ◽  
And Function ◽  
Ubiquitous Learning Environment ◽  
Very High ◽  
Data Flow Model

WMSNs is one of the most cutting-edge technologies of the field of informatics, and compared to other sensor technology has broad prospects for development. WMSNs has very high service quality, larger multimedia data transmission amount, multimedia data flow model, a wide variety of application services and other characteristics. WMSNs support for education and teaching in support of ubiquitous learning environment. According to the characteristic of WMSNs, expounds the WMSNs support ubiquitous learning environment and function principle, as well as WMSNs ubiquitous learning environment application.

scholarly journals Early neonatal mortality rate and the risk factors in Wangaya hospital

2016 ◽  
Vol 48 (5) ◽  
pp. 306
Author(s):  
Made Lndah Nastiti Utami Budha ◽  
Wayan Retayasa ◽  
Made Kardana
Keyword(s):  
Risk Factors ◽  
Birth Weight ◽  
Mortality Rate ◽  
Neonatal Mortality ◽  
Gestational Age ◽  
Neonatal Death ◽  
Early Neonatal Death ◽  
Neonatal Mortality Rate ◽  
Early Neonatal Mortality ◽  
Early Neonatal Mortality Rate

Background The first week of life of a neonate is a critical period.In Asia, early neonatal mortality rate remains high.Objective To investigate early neonatal mortality rate and the riskfactors in Wangaya Hospital.Methods A cross sectional study was carried out retrospectivelyon neonates registered at Perinatology Unit, Wangaya HospitalDenpasar, Bali since January 2006. The study was done fromOctober to November 2007. Data was obtained from medicalrecord, analyzed as univariate using chi-square test or Fisher'sexact test and multivariate logistic regression analysis model.Results Early neonatal mortality rate in Wangaya Hospital was 38.7per 1000 livebirths. Univariate analyses showed that there werefive significant risk factors of early neonatal death, i.e., respiratorydistress, asphyxia, birth weight less than 2500 grams, sepsis, andgestational age less than 3 7 weeks. Multivariate analysis showedthat those five variables were significant as risk factors of earlyneonatal death i.e., OR (95% confidence interval) for respiratorydistress: 16.8 (3.7 to 76.6)], asphyxia: 13.5 (6.1 to 29.9)], birthweight <2500 grams: 8.1 (3.3 to 19.9)], sepsis: 7.3 (3.1 to 17.1),and gestational age <37 weeks: 3.5 (1.6 to 7.8)].Conclusions Early neonatal mortality rate in Wangaya Hospitalremains high. Respiratory distress, asphyxia, birth weight <2500gram, sepsis, and gestational age <37 weeks were independent riskfactors of early neonatal death.

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