Rho-GTPase effector ROCK regulates mouse oocyte maturation and embryo development

Abstract Oocyte maturation is a prerequisite for successful fertilization and embryo development. Incomplete oocyte maturation can result in infertility. Ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) has been found to be implicated in oocyte maturation and embryo development. However, the cellular and molecular mechanisms of UCH-L1 underlying oocyte maturation have not been fully elucidated. In the present study, we observed that the introduction of UCH-L1 inhibitor LDN-57444 suppressed first polar body extrusion during mouse oocyte maturation. The inhibition of UCH-L1 by LDN-57444 led to the notable increase in reactive oxygen species (ROS) level, conspicuous reduction in glutathione (GSH) content and mitochondrial membrane potential (MMP), and blockade of spindle body formation. As a conclusion, UCH-L1 inhibitor LDN-57444 suppressed mouse oocyte maturation by improving oxidative stress, attenuating mitochondrial function, curbing spindle body formation and down-regulating extracellular signal-related kinases (ERK1/2) expression, providing a deep insight into the cellular and molecular basis of UCH-L1 during mouse oocyte maturation.

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The Effects of Vero Cell Coculture on Mouse Oocyte Maturation and Embryo Development in Vitro

Ewha Medical Journal ◽

10.12771/emj.1996.19.3.425 ◽

1996 ◽

Vol 19 (3) ◽

pp. 425

Author(s):

Hye Na Kang ◽

Jong-Sik Hah

Keyword(s):

Vero Cell ◽

Embryo Development ◽

Oocyte Maturation ◽

Mouse Oocyte ◽

Development In Vitro

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FOXO1, FOXO3, AND FOXO4 are differently expressed during mouse oocyte maturation and preimplantation embryo development

Gene Expression Patterns ◽

10.1016/j.gep.2015.04.003 ◽

2015 ◽

Vol 18 (1-2) ◽

pp. 16-20 ◽

Cited By ~ 15

Author(s):

Nilay Kuscu ◽

Ciler Celik-Ozenci

Keyword(s):

Embryo Development ◽

Oocyte Maturation ◽

Preimplantation Embryo ◽

Mouse Oocyte ◽

Preimplantation Embryo Development

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Absence of mitochondrial DNA methylation in mouse oocyte maturation, aging and early embryo development

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2019.04.100 ◽

2019 ◽

Vol 513 (4) ◽

pp. 912-918 ◽

Cited By ~ 3

Author(s):

Li-Hua Fan ◽

Zhen-Bo Wang ◽

Qian-Nan Li ◽

Tie-Gang Meng ◽

Ming-Zhe Dong ◽

...

Keyword(s):

Dna Methylation ◽

Mitochondrial Dna ◽

Embryo Development ◽

Oocyte Maturation ◽

Early Embryo ◽

Mouse Oocyte ◽

Early Embryo Development

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The role of propofol on mouse oocyte meiotic maturation and early embryo development

Zygote ◽

10.1017/s0967199418000163 ◽

2018 ◽

Vol 26 (4) ◽

pp. 261-269

Author(s):

Xia-Guang Duan ◽

Zai-Qing Huang ◽

Chun-Guang Hao ◽

Xiao-Jun Zhi ◽

Xiao-Bing Qi ◽

...

Keyword(s):

Embryo Development ◽

Oocyte Maturation ◽

Polar Body ◽

Oocyte Retrieval ◽

Early Embryo ◽

Mouse Oocyte ◽

Early Embryo Development ◽

Intravenous Anaesthetic ◽

Polar Body Extrusion

SummaryPropofol is a intravenous anaesthetic most commonly used in ultrasound oocyte retrieval. We studied if the use of propofol had an effect on mouse oocyte maturation, pregnancy, childbirth and progeny and investigated the correlation between propofol side effects and reproductive performance in mice. There was no statistical difference in mating, pregnancy, childbirth, litter size, the number of stillbirths and survival between each group (P>0.05). Propofol also had no effect on polar body extrusion in oocyte maturation as well as on pronucleus formation and, subsequently, early embryo development (P>0.05). An increased concentration of propofol had no effect on this result, although propofol at more than 0.01 mg/ml reduced polar body extrusion. Different concentrations of propofol had no effect on oocyte culture in vitro, pronucleus formation and early embryo development.

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