scholarly journals Rice TSV3 Encoding Obg-Like GTPase Protein Is Essential for Chloroplast Development During the Early Leaf Stage Under Cold Stress

2017 ◽  
Vol 8 (1) ◽  
pp. 253-263 ◽  
Author(s):  
Dongzhi Lin ◽  
Quan Jiang ◽  
Xiaojing Ma ◽  
Kailun Zheng ◽  
Xiaodi Gong ◽  
...  
Keyword(s):  
Cold Stress ◽  
Chloroplast Development ◽  
Leaf Stage

scholarly journals Rice TSV2 Encoding Threonyl-tRNA Synthetase is Needed for Early Chloroplast Development and Seedling Growth under Cold Stress

2021 ◽  
Author(s):  
Dongzhi Lin ◽  
Wenhao Zhou ◽  
Yulu Wang ◽  
Jia Sun ◽  
Xiaobiao Pan ◽  
...  
Keyword(s):  
Cold Stress ◽  
Chloroplast Development ◽  
Chlorophyll Biosynthesis ◽  
Trna Synthetase ◽  
Loss Of Function ◽  
Specific Expression ◽  
Trna Synthetases ◽  
Leaf Stage ◽  
Virescent Mutant ◽  
Albino Phenotype

Abstract Threonyl-tRNA synthetase (ThrRS), one of aminoacyl-tRNA synthetases (AARSs), plays a crucial role in protein synthesis. However, the AARS functions on rice chloroplast development and growth were not fully appraised. In this study, a thermo-sensitive virescent mutant tsv2, which showed albino phenotype and lethal after the 4-leaf stage at 20 °C but recovered to normal when the temperatures rose, was identified and characterized. Map-based cloning and complementation tests showed that TSV2 encoded a chloroplast-located ThrRS protein in rice. The Lys-to-Arg mutation in the anticodon-binding domain hampered chloroplast development under cold stress, while the loss-of-function of the ThrRS core domain in TSV2 fatally led to seedling death regardless of growing temperatures. In addition, TSV2 had a specific expression in early leaves. Its disruption obviously resulted in down-regulation of certain genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development at cold conditions. Our observations revealed that rice nuclear-encoded TSV2 plays an important role in chloroplast development at the early leaf stage under cold stress.

scholarly journals RiceTSV3Encoding Obg-like GTPase Protein is Essential for Chloroplast Development during the Early Leaf Stage under Cold Stress

2017 ◽  
Author(s):  
Dongzhi Lin ◽  
Quan Jiang ◽  
Xiaojing Ma ◽  
Kailun Zheng ◽  
Xiaodi Gong ◽  
...  
Keyword(s):  
Cold Stress ◽  
Chloroplast Development ◽  
Higher Plants ◽  
Complementation Test ◽  
Specific Expression ◽  
Leaf Stage ◽  
Gtp Binding ◽  
Normal Green ◽  
Virescent Mutant ◽  
Higher Temperature

ABSTRACTThe Spo0B-associated GTP-binding (Obg) proteins occupy a wide variety of roles in the viability of nearly all bacteria. Its detailed roles in higher plants have not yet been elucidated. A novel rice thermo-sensitive virescent mutanttsv3was identified in this study that displayed albino phenotype at 20°C before the 3-leaf stage while being normal green at 32°C or even at 20°C after the 4-leaf stage. The mutant phenotype was aligned with altered chlorophyll (Chl) content and chloroplast development. Map-based cloning and complementation test showed thatTSV3encoded a kind of small GTP binding protein. Subcellular localization revealed that TSV3 was in chloroplast.TSV3transcripts were highly expressed in leaves and weak or undetectable in other tissues, suggesting the tissue-specific expression. Intsv3mutant, the transcriptional levels of certain genes associated with biogenesis of chloroplast ribosomes 50S subunit were severely decreased at the 3-leaf-stage under cold stress, but could be recovered to normal levels at a higher temperature (32°C). The observations from this study indicated that the rice nuclear-encodedTSV3plays important roles in chloroplast development at early leaf stage under cold stress.

A DEAD-box RNA helicase TCD33 that confers chloroplast development in rice at seedling stage under cold stress

2020 ◽  
Vol 248 ◽  
pp. 153138 ◽  
Author(s):  
Wang Xiaomei ◽  
Kong Rongrong ◽  
Zhang Ting ◽  
Gao Yuanyuan ◽  
Xu Jianlong ◽  
...  
Keyword(s):  
Cold Stress ◽  
Chloroplast Development ◽  
Rna Helicase ◽  
Seedling Stage ◽  
Dead Box

scholarly journals The Component of the TAC Complex, TCD7, Controls Rice Chloroplast Development at the Early Seedling Stage under Cold Stress

2021 ◽  
Author(s):  
Dongzhi Lin ◽  
Licheng Kang ◽  
Wenhao Zhou ◽  
Yulu Wang ◽  
Yu Chen ◽  
...  
Keyword(s):  
Cold Stress ◽  
Low Temperatures ◽  
Chloroplast Development ◽  
Oryza Sativa L ◽  
Polymerase Activity ◽  
Regulatory Module ◽  
Complex Functions ◽  
In The Wild ◽  
Early Seedling ◽  
Albino Phenotype

Abstract Transcriptionally active chromosome (TAC) is a component of protein-DNA complexes with RNA polymerase activity found in chloroplasts. Although TAC in Arabidopsis thaliana has been extensively investigated, how the rice (Oryza sativa L.) TAC complex functions remains largely unknown. We report the characterization of the mutant thermosensitive chlorophyll-deficient7 (tcd7) and the cloning of TCD7. tcd7 mutant seedlings displayed an albino phenotype specifically at low temperatures and before the four-leaf stage. We identified TCD7 by map-based cloning followed by transgenic rescue and genome editing tests, showing that TCD7 encodes the putative TAC component FRUCTOKINASE-LIKE 2 (OsFLN2). TCD7 transcripts were highly abundant in green tissues, and the protein localized to chloroplasts. In agreement with the albino phenotype, transcript levels of genes controlling chloroplast development and the establishment of photosynthetic capacity were severely reduced in tcd7 seedlings at low temperatures, but were expressed as in the wild type at high temperatures, implying that TCD7 regulates the PEP pathway and chloroplast development. Moreover, TCD7 interacted with the thioredoxin OsTRXz to form an OsTRXz-TCD7 regulatory module, which might regulate plastid transcription under cold stress. Our results demonstrate that the nucleus-encoded TAC protein TCD7 protects chloroplast development from cold stress via a TRXz-FLN regulatory module.

scholarly journals SPLICING FACTOR1 Is Important in Chloroplast Development under Cold Stress

2020 ◽  
Vol 184 (2) ◽  
pp. 973-987
Author(s):  
Yajuan Zhu ◽  
Wenjuan Wu ◽  
Wei Shao ◽  
Jingli Chen ◽  
Xiaoning Shi ◽  
...  
Keyword(s):  
Cold Stress ◽  
Chloroplast Development

scholarly journals The Rice Pentatricopeptide Repeat Gene TCD10 is Needed for Chloroplast Development under Cold Stress

Rice ◽  
2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Lanlan Wu ◽  
Jun Wu ◽  
Yanxia Liu ◽  
Xiaodi Gong ◽  
Jianlong Xu ◽  
...  
Keyword(s):  
Cold Stress ◽  
Chloroplast Development ◽  
Pentatricopeptide Repeat

Rice TSV2 Encoding Threonyl-tRNA Synthetase is Needed for Early Chloroplast Development and Seedling Growth under Cold Stress

2020 ◽  
Author(s):  
Dongzhi Lin ◽  
Wenhao Zhou ◽  
Jia Sun ◽  
Yulu Wang ◽  
Xiaobiao Pan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Cold Stress ◽  
Chloroplast Development ◽  
Chlorophyll Biosynthesis ◽  
Trna Synthetase ◽  
Loss Of Function ◽  
Specific Expression ◽  
Trna Synthetases ◽  
Virescent Mutant ◽  
Albino Phenotype

Abstract Background The chloroplast is a vital photosynthetic organelle for plant growth and development. However, the genetic factors involved in chloroplast development and its relationship with environment factors are largely unknown. Threonyl-tRNA synthetase (ThrRS), one of aminoacyl-tRNA synthetases (AARSs), plays a crucial role in protein synthesis. To date, there are few studies for AARS function on chloroplast development and plant growth, much less ThrRS in rice. Result In this paper, we characterized a thermo-sensitive virescent mutant tsv2, which showed albino phenotype and could not survive after the 4-leaf stage when grown at 20 °C, but recovered the normal phenotype when the temperature rose. Map-based cloning and complementation tests showed that TSV2 encoded a chloroplast-located ThrRS protein in rice and the Lys-to-Arg mutation in the anticodon-binding domain affected chloroplast development under cold stress. Furthermore, the loss-of-function of the core domain in TSV2 led to seedling death regardless of temperatures. In addition, TSV2 had a tissue-specific expression, and its disruption resulted in an evidently down-regulation of certain genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development at cold stress. Conclusion The TSV2 encodes a rice threonyl-tRNA synthetase, located in chloroplasts, which is essential for cold-responsive regulation for chloroplast development and plant growth and closely related to the assembly of chloroplast ribosomes and functions at the first step of chloroplast differentiation.

A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions

2011 ◽  
Vol 68 (6) ◽  
pp. 1039-1050 ◽  
Author(s):  
Kensuke Kusumi ◽  
Chikako Sakata ◽  
Takahiro Nakamura ◽  
Shinji Kawasaki ◽  
Atsushi Yoshimura ◽  
...  
Keyword(s):  
Cold Stress ◽  
Chloroplast Development ◽  
Genetic System ◽  
Stress Conditions ◽  
Plastid Protein
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