The Morphology and Histology of The Female Reproductive System of Graphosoma lineatum (Heteroptera: Pentatomidae) based on light and scanning electron microscope studies

2012 ◽  
Vol 2 (12) ◽  
pp. 42-46 ◽  
Author(s):  
Nurcan ÖZYURT ◽  
◽  
Selami CANDAN ◽  
Zekiye SULUDERE
1982 ◽  
Vol 72 (3) ◽  
pp. 403-408 ◽  
Author(s):  
Linda C. Haines

AbstractExternal sexual characters are described for the larvae of Spodoptera littoralis (Boisd) and S. exempta (Wlk.). The characters on the female consist of two pairs of pits on the ventral surface of the eighth and ninth abdominal segments. These pits are visible under a light microscope and can therefore be used for the accurate sexing of live larvae from the third instar onwards. The first- and second-instar larvae of both species could not be sexed using external characters because of their small size. In the males of both species, there are no obvious external sexual characters until the last instar, and therefore the sexes are most easily separated by the presence or absence of the female characters. Third- to sixth-instar larvae of both species were examined for the presence or absence of the female pits and were then sexed by dissection. The accuracy of sexing using the external characters was 90–98% for S. littoralis and 100% in all cases for S. exempta. The histology of the female pits and their appearance under the scanning electron microscope is described. These pits in the female larvae give rise to the external openings of the adult reproductive system.


Nematology ◽  
2003 ◽  
Vol 5 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Oleksandr Holovachov ◽  
Alejandro Esquivel ◽  
Tom Bongers

Abstract Four species of Cephalobina: Geraldius bakeri, Diastolaimus croca, Trualaimus culeatus and Tricirronema tentaculatum are described and illustrated on the basis of material collected in Costa Rica. A scanning electron microscope (SEM) study of G. bakeri is given for the first time. Diastolaimus croca is found to have a circle of six setiform processes anterior to the true labial setae. Detailed descriptions of the female reproductive system in G. backeri, D. croca and Macrolaimus sp. are given. The use of the female reproductive system and structure of the labial sensilla in the systematics of the family Chambersiellidae, together with its taxonomic placement, are discussed. Notes on the systematic position of the family Bicirronematidae and a key to the species of this family are given.


Author(s):  
R. E. Ferrell ◽  
G. G. Paulson

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.


Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.


Author(s):  
K. Shibatomi ◽  
T. Yamanoto ◽  
H. Koike

In the observation of a thick specimen by means of a transmission electron microscope, the intensity of electrons passing through the objective lens aperture is greatly reduced. So that the image is almost invisible. In addition to this fact, it have been reported that a chromatic aberration causes the deterioration of the image contrast rather than that of the resolution. The scanning electron microscope is, however, capable of electrically amplifying the signal of the decreasing intensity, and also free from a chromatic aberration so that the deterioration of the image contrast due to the aberration can be prevented. The electrical improvement of the image quality can be carried out by using the fascionating features of the SEM, that is, the amplification of a weak in-put signal forming the image and the descriminating action of the heigh level signal of the background. This paper reports some of the experimental results about the thickness dependence of the observability and quality of the image in the case of the transmission SEM.


Author(s):  
S. Takashima ◽  
H. Hashimoto ◽  
S. Kimoto

The resolution of a conventional transmission electron microscope (TEM) deteriorates as the specimen thickness increases, because chromatic aberration of the objective lens is caused by the energy loss of electrons). In the case of a scanning electron microscope (SEM), chromatic aberration does not exist as the restrictive factor for the resolution of the transmitted electron image, for the SEM has no imageforming lens. It is not sure, however, that the equal resolution to the probe diameter can be obtained in the case of a thick specimen. To study the relation between the specimen thickness and the resolution of the trans-mitted electron image obtained by the SEM, the following experiment was carried out.


Author(s):  
R. F. Schneidmiller ◽  
W. F. Thrower ◽  
C. Ang

Solid state materials in the form of thin films have found increasing structural and electronic applications. Among the multitude of thin film deposition techniques, the radio frequency induced plasma sputtering has gained considerable utilization in recent years through advances in equipment design and process improvement, as well as the discovery of the versatility of the process to control film properties. In our laboratory we have used the scanning electron microscope extensively in the direct and indirect characterization of sputtered films for correlation with their physical and electrical properties.Scanning electron microscopy is a powerful tool for the examination of surfaces of solids and for the failure analysis of structural components and microelectronic devices.


Author(s):  
S. Saito ◽  
H. Todokoro ◽  
S. Nomura ◽  
T. Komoda

Field emission scanning electron microscope (FESEM) features extremely high resolution images, and offers many valuable information. But, for a specimen which gives low contrast images, lateral stripes appear in images. These stripes are resulted from signal fluctuations caused by probe current noises. In order to obtain good images without stripes, the fluctuations should be less than 1%, especially for low contrast images. For this purpose, the authors realized a noise compensator, and applied this to the FESEM.Fig. 1 shows an outline of FESEM equipped with a noise compensator. Two apertures are provided gust under the field emission gun.


Author(s):  
Emil Bernstein

An interesting method for examining structures in g. pig skin has been developed. By modifying an existing technique for splitting skin into its two main components—epidermis and dermis—we can in effect create new surfaces which can be examined with the scanning electron microscope (SEM). Although this method is not offered as a complete substitute for sectioning, it provides the investigator with a means for examining certain structures such as hair follicles and glands intact. The great depth of field of the SEM complements the technique so that a very “realistic” picture of the organ is obtained.


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