scholarly journals Tyrosinase activity and melanogenic effects of Lespedeza bicolor extract in vitro and in vivo

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6244-6261
Author(s):  
Si Young Ha ◽  
Ji Young Jung ◽  
Hee Young Kang ◽  
Tae-Heung Kim ◽  
Jae-Kyung Yang
Keyword(s):  
Ethanol Extract ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
Melanin Content ◽  
B16 Melanoma Cells ◽  
Notable Increase ◽  
Lespedeza Bicolor

Lespedeza bicolor (L. bicolor) is used for medicinal purposes because of its various biological and pharmacological activities. In this study, the effects of L. bicolor ethanol extract on the treatment of vitiligo were investigated. The determination of melanin content in melanocytes was measured using B16 melanoma cells and C57BL/6J Ler-vit/vit mice. Finally, the quercetin content in L. bicolor were qualitatively analyzed using HPLC. The results obviously indicated that the L. bicolor extract enhanced melanogenesis and increased tyrosinase activity in cultured melanoma cells and C57BL/6J Ler-vit/vit mice. Treatment with L. bicolor extract led to a higher content of melanin and eumelanin in C57BL/6J Ler-vit/vit mice hair than in control (untreated) mice, which demonstrated the therapeutic effect of hair-graying associated with vitiligo. There was a notable increase in melanocytes in the skin of C57BL/6J Ler-vit/vit mice treated with L. bicolor extract compared with the control. L. bicolor extract was a potent tyrosinase and melanin synthesis activator in B16 melanoma cells. C57BL/6J Ler-vit/vit mice treated with L. bicolor extract had significantly higher melanin content in hair than the untreated control. The results suggest that L. bicolor extract is a potential alternative treatment for improvement of vitiligo.

scholarly journals Osmanthus fragrans Flower Aqueous Extract and its Enriched Acteoside inhibit Melanogenesis and Ultraviolet-induced Pigmentation

2018 ◽  
Vol 13 (5) ◽  
pp. 1934578X1801300
Author(s):  
Shuo Liu ◽  
Zhen Zhao ◽  
Zhijun Huo ◽  
Zhiru Xu ◽  
Yan Zhong ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Ex Vivo ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
In Vivo Model ◽  
Mrna Levels ◽  
Osmanthus Fragrans ◽  
B16 Melanoma Cells

The Osmanthus fragrans flower (OFF) is commonly used as an additive for tea in China and as a traditional medicine to treat dysentery, asthma and hepatitis. In the current study, we have acquired the aqueous extract of the dried OFF (OFFE) and determined its enriched acteoside contents. However, whether OFFE and acteoside can modulate melanogenesis and pigmentation has yet to be determined. We here provide novel data revealing that OFFE and acteoside inhibit melanogenesis induced by α-MSH in B16 melanoma cells via the MITF-tyrosinase signaling pathway. Treatment with α-MSH (1μM) enhanced melanin levels and tyrosinase activity, up-regulated the mRNA levels of MITF and tyrosinase and increased the dendritic number in B16 melanoma cells, effects all being intervened by OFFE and acteoside. Of interest, OFFE and acteoside showed no direct inhibition of tyrosinase activity as revealed by our ex vivo tyrosinase activity assay. In addition, OFFE produced a depigmenting action on UVB-induced hyperpigmentation in guinea pigs, as shown by the improved skin brightness and the decreased melanin staining. Our data have demonstrated that OFFE can alter melanogenesis via modulating the MITF-tyrosinase signaling thereby leading to its depigmenting action in the in vivo model. OFFE could be a substitute for acteoside as a promising skin-whitening agent.

In vitro and in vivo Enhancement of Vincristine Antitumor Activity on B16 Melanoma Cells by Calcium Antagonist Flunarizine

Oncology ◽  
1987 ◽  
Vol 44 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Alberto Bellelli ◽  
Claudia Camboni ◽  
Giovanna de Luca ◽  
Mario Materazzi ◽  
Manlio Mattioni ◽  
...  
Keyword(s):  
Calcium Antagonist ◽  
Antitumor Activity ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
B16 Melanoma Cells

Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study

2008 ◽  
Vol 46 (5) ◽  
pp. 1825-1833 ◽  
Author(s):  
Lj. Harhaji ◽  
S. Mijatović ◽  
D. Maksimović-Ivanić ◽  
I. Stojanović ◽  
M. Momčilović ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Coriolus Versicolor ◽  
In Vivo Study ◽  
B16 Melanoma Cells

Artocarpus Plants as a Potential Source of Skin Whitening Agents

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Enos Tangke Arung ◽  
Kuniyoshi Shimizu ◽  
Ryuichiro Kondo
Keyword(s):  
Inhibitory Activity ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Vitro Assay ◽  
Skin Whitening ◽  
Melanin Formation ◽  
B16 Melanoma Cells ◽  
Structure Activity

Artocarpus plants have been a focus of constant attention due to the potential for skin whitening agents. In the in vitro experiment, compounds from the Artocarpus plants, such as artocarpanone, norartocarpetin, artocarpesin, artogomezianol, andalasin, artocarbene, and chlorophorin showed tyrosinase inhibitory activity. Structure-activity investigations revealed that the 4-substituted resorcinol moiety in these compounds was responsible for their potent inhibitory activities on tyrosinase. In the in vitro assay, using B16 melanoma cells, the prenylated polyphenols isolated from Artocarpus plants, such as artocarpin, cudraflavone C, 6-prenylapigenin, kuwanon C, norartocarpin, albanin A, cudraflavone B, and brosimone I showed potent inhibitory activity on melanin formation. Structure-activity investigations revealed that the introduction of an isoprenoid moiety to a non-isoprenoid-substituted polyphenol enhanced the inhibitory activity of melanin production in B16 melanoma cells. In the in vivo investigation, the extract of the wood of Artocarpus incisus and a representative isolated compound from it, artocarpin had a lightening effect on the skin of guinea pigs’ backs. Other in vivo experiments using human volunteers have shown that water extract of Artocarpus lakoocha reduced the melanin formation in the skin of volunteers. These results indicate that the extracts of Artocarpus plants are potential sources for skin whitening agents.

TNF Impairs In Vivo and In Vitro Natural Killer (NK) Susceptibility of B16 Melanoma Cells

1992 ◽  
Vol 35 (3) ◽  
pp. 279-287 ◽  
Author(s):  
G. PALMIERI ◽  
S. MORRONE ◽  
P.-L. LOLLINI ◽  
C. GIOVANNI ◽  
G. NICOLETTI ◽  
...  
Keyword(s):  
Natural Killer ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
B16 Melanoma Cells
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