scholarly journals Caracterización molecular ómica de una cepa de Bacillus amyloliquefaciens aislada de la microbiota del paiche Arapaima gigas con actividad antagonista contra bacterias patógenas de peces

2019 ◽  
Vol 30 (2) ◽  
pp. 908-922
Author(s):  
Manuel Alberto Feria Zevallos ◽  
Arnaldo Castañeda ◽  
Odalis Toledo ◽  
Deysy Caballero ◽  
Mario Cueva ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Bacillus Amyloliquefaciens ◽  
Iturin A ◽  
Maldi Tof ◽  
Arapaima Gigas ◽  
Bacillomycin L

Se analizaron las características de una cepa de Bacillus amyloliquefaciens aislada del intestino de Arapaima gigas, seleccionada por su capacidad para generar inhibición de múltiples bacterias patógenas de peces, mediante el uso de herramientas moleculares como PCR y espectrometría de masas - MALDI TOF/TOF. Los resultados mostraron a través de PCR que esta cepa cuenta con genes claves para la generación de péptidos antimicrobianos como bmyB (bacillomycin L synthetase B), fenD (fengycin sintetasa), srfAA (subunidad 1 surfactin sintetasa), bacA (proteína de biosíntesis de bacilysin) e iturin (iturin A). Además, mediante el análisis por espectrometría de masas se detectaron bacteriocinas (plipastatin, gramicidin, fengycin, surfactin), proteínas de fijación al intestino (like-enolase), proteinas transportadoras de péptidos antimicrobianos (ABC-transporters) y proteínas de estimulación del sistema inmunitario como flagelin. También se detectaron proteínas tipo colagenasas, chitinasas e xilosa-isomerasas que contribuyen con el proceso de digestión y asimilación. Todos estos resultados permiten considerar los múltiples beneficios de esta cepa para ser utilizada como probiótico en el cultivo de peces.

scholarly journals Statistical optimization of antifungal iturin A production from Bacillus amyloliquefaciens RHNK22 using agro-industrial wastes

2017 ◽  
Vol 24 (7) ◽  
pp. 1722-1740 ◽  
Author(s):  
P. Narendra Kumar ◽  
T.H. Swapna ◽  
Mohamed Yahya Khan ◽  
Gopal Reddy ◽  
Bee Hameeda
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Statistical Optimization ◽  
Industrial Wastes ◽  
Iturin A

Kinetic analysis on precursors for iturin A production from Bacillus amyloliquefaciens BPD1

2018 ◽  
Vol 126 (5) ◽  
pp. 630-635 ◽  
Author(s):  
Jiun-Yan Wu ◽  
Jen-Hung Liao ◽  
Chwen-Jen Shieh ◽  
Feng-Chia Hsieh ◽  
Yung-Chuan Liu
Keyword(s):  
Kinetic Analysis ◽  
Bacillus Amyloliquefaciens ◽  
Iturin A

Enhanced production of iturin A in Bacillus amyloliquefaciens by genetic engineering and medium optimization

2020 ◽  
Vol 90 ◽  
pp. 50-57
Author(s):  
Yuxiang Xu ◽  
Dongbo Cai ◽  
Hong Zhang ◽  
Lin Gao ◽  
Yong Yang ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Bacillus Amyloliquefaciens ◽  
Medium Optimization ◽  
Iturin A ◽  
Enhanced Production

scholarly journals Antagonistic Mechanism of Iturin A and Plipastatin A from Bacillus amyloliquefaciens S76-3 from Wheat Spikes against Fusarium graminearum

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0116871 ◽  
Author(s):  
An-Dong Gong ◽  
He-Ping Li ◽  
Qing-Song Yuan ◽  
Xiu-Shi Song ◽  
Wei Yao ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Bacillus Amyloliquefaciens ◽  
Iturin A

scholarly journals Caracterización de la secuencia del gen de la proteína transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas

2019 ◽  
Vol 30 (3) ◽  
pp. 1129-1142
Author(s):  
Odalis Epifanía Toledo Valdiviezo ◽  
Arnaldo Castañeda ◽  
Manuel A. Feria ◽  
Pedro Masías ◽  
Mario Cueva ◽  
...  
Keyword(s):  
Maldi Tof ◽  
Arapaima Gigas

El objetivo del estudio fue caracterizar el PepT1 del paiche Arapaima gigas mediante herramientas genómicas y proteómicas. Se realizó un análisis transcriptómico y proteómico a partir de secciones del intestino, bazo, hígado y riñón de alevines. El análisis transcriptómico permitió obtener dos secuencias consenso de 357 y 459 pb. Ambos fragmentos presentaron un alto grado de homología (78 y 80%), principalmente con secuencias de nucleótidos codificantes del PepT1 de Scleropages formosus del mismo Orden que el paiche. El análisis proteómico por espectrometría de doble masa MALDI TOF/TOF permitió identificar quince secuencias peptídicas del PepT1 en paiche. Como conclusión se logró caracterizar parcialmente el PepT1 en el intestino del paiche, las cuales podrán ser usadas para posteriores estudios sobre la evaluación de su expresión.

scholarly journals Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenchao Chen ◽  
Xuan Li ◽  
Xuli Ma ◽  
Shouwen Chen ◽  
Yanping Kang ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Bacillus Amyloliquefaciens ◽  
Rapeseed Oil ◽  
Low Cost ◽  
Rapeseed Meal ◽  
Raw Material ◽  
Negative Effects ◽  
Iturin A ◽  
Rapeseed Cake ◽  
Cyclic Lipopeptide

Abstract Background Rapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, RSC has been rarely used as a raw material for microbial fermentation, although both RSC and RSM are mainly composed of proteins, carbohydrates and minerals. In this study, we investigated the feasibility of using untreated low-cost RSC as nitrogen source to produce the valuable cyclic lipopeptide antibiotic iturin A using Bacillus amyloliquefaciens CX-20 in submerged fermentation. Especially, the effect of oil in RSC on iturin A production and the possibility of using lipases to improve the iturin A production were analyzed in batch fermentation. Results The maximum production of iturin A was 0.82 g/L at the optimal initial RSC and glucose concentrations of 90 and 60 g/L, respectively. When RSC was substituted with RSM as nitrogen source based on equal protein content, the final concentration of iturin A was improved to 0.95 g/L. The production of iturin A was further increased by the addition of different lipase concentrations from 0.1 to 5 U/mL into the RSC medium for simultaneous hydrolysis and fermentation. At the optimal lipase concentration of 0.5 U/mL, the maximal production of iturin A reached 1.14 g/L, which was 38.15% higher than that without any lipase supplement. Although rapeseed oil and lipase were firstly shown to have negative effects on iturin A production, and the effect would be greater if the concentration of either was increased, their respective negative effects were reduced when used together. Conclusions Appropriate relative concentrations of lipase and rapeseed oil were demonstrated to support optimal iturin A production. And simultaneous hydrolysis with lipase and fermentation was an effective way to produce iturin A from RSC using B. amyloliquefaciens CX-20.

Purification and Partial Characterization of Bacillomycin L Produced by Bacillus amyloliquefaciens K103 from Lemon

2013 ◽  
Vol 171 (8) ◽  
pp. 2262-2272 ◽  
Author(s):  
Bao Zhang ◽  
Chunjuan Dong ◽  
Qingmao Shang ◽  
Yuan Cong ◽  
Weijia Kong ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Partial Characterization ◽  
Bacillomycin L
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