БИОЛОГИЧЕСКИЕ ПАРАМЕТРЫ ПИЩЕВАРИТЕЛЬНЫХ И ОБМЕННЫХ ПРОЦЕССОВ У МЕЖВИДОВЫХ ГИБРИДОВ ДОМАШНЕЙ ОВЦЫ (Ovis aries) И АРХАРА (Ovis ammon polii)

Neuroglobin (Ngb) is a member of the globin member that is mainly expressed in the brain and retina. The expression in the retina is greater than it is in the brain because of the high demand for oxygen by the retina. This protein reversibly binds oxygen and is involved in oxygen transport or protection against oxidative stress. The study aims to explore the pattern of Ngb expression in Tibetan sheep (Ovis ammon or argali ) living in a plateau region and domestic sheep (Ovis aries) dwelling on low land. The study also provides detailed insight into the morphological characteristics of Ngb distribution closely related to oxygen metabolism in the retina of Tibetan and domestic sheep. Immunohistochemical staining procedures were performed to detect the Ngb protein expression in the retinae of both sheep, while the Inter’s integrated performance primitives (IPP) semi-quantitative analysis was employed to compare the quantities of Ngb expressed in the retinae of the two types of sheep. The outer nuclear layer and the outer segment of the photoreceptor showed a negative Ngb expression, while a weak positive expression was observed in the inner core layer and ganglion cell layer, while a strong positive expression was found in the other layers. The independent T-test revealed that the expression intensity in the nerve fiber layer, inner and outer plexus layers, inner segment of the photoreceptor, and superior pigment cortex were significantly higher in the Tibetan sheep as compared to the domestic sheep. The overall Ngb expression in the Tibetan sheep was higher than the domestic sheep. The study emphasized that the different Ngb expression in the Tibetan and domestic sheep may influence their adaptation to the respective environments. The stronger Ngb expression in the retina of Tibetan sheep may indicate that Ngb is closely related to the oxygen metabolism of these animals and has a function during oxygen transport.

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Analysis of parameters characterizing argyrophilic zones in intact lymphocytes of domestic sheep (Ovis aries L., 1758) and their hybrids with argali (Ovis ammon L., 1758)

Agrarian science ◽

10.32634/0869-8155-2021-344-1-52-56 ◽

2021 ◽

Vol 344 (1) ◽

pp. 52-56

Author(s):

P. M. Klenovitsky ◽

B. S. Iolchiev ◽

A. N. Vetokh

Keyword(s):

Peripheral Blood Lymphocytes ◽

Ovis Aries ◽

F1 Hybrids ◽

Domestic Sheep ◽

Functional Evaluation ◽

Group 4 ◽

Average Color ◽

Color Density ◽

Group 2 ◽

Ovis Ammon

Relevance and research material. The study of the characteristics of the nucleolus in cells of various tissues allows us to assess the intensity of the processes of protein proliferation and biosynthesis in animals. In interphase cells, the nucleoli correspond to argyrophilic zones (AgNOR) stained with silver nitric acid. The purpose of this study is to determine the characteristics of AgNOR in sheep interphase lymphocytes of different genotypes based on computer analysis and to select parameters for functional evaluation of nucleoli. AgNOR status was studied in peripheral blood lymphocytes from hybrid sheep four genotypes: purebred Romanov sheep, and their F1 hybrids with argali (groups 1 and 2), hybrids, bearing 3/4 of Romanov sheep blood and 1/4 blood argali (group 3), and hybrids that had 7/8 of domestic sheep and 1/8 blood argali (group 4). The number of argyrophilic zones (AgNOR), their total area (SSNOR), their average color density (DNOR), and the average color density of the core (DN) and its AgNOR-free area (DF) were taken into account.Results. Image processing and analysis were performed using the Image Scope 1.0 program. The average AgNOR value in the studied groups of sheep ranges from 2.64 (group 1) to 3.50 in hybrids in groups 2 and 3. There were no significant differences in the value of SSNOR between the groups. In terms of DN and DF, all hybrids were significantly inferior to purebred sheep (р < 0.001). In terms of DNOR, the sheep of group 2 were significantly inferior to the animals of the other groups (р < 0.001). When evaluating the state of the nucleolus-forming system, it is advisable to take into account the AgNOR number, as well as the average optical densities DN and DF.

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Sarcocystis species in wild and domestic sheep (Ovis ammon and Ovis aries) from China

BMC Veterinary Research ◽

10.1186/s12917-018-1712-9 ◽

2018 ◽

Vol 14 (1) ◽

Cited By ~ 4

Author(s):

Hui Dong ◽

Ruijing Su ◽

Yinghua Wang ◽

Zongxi Tong ◽

Longxian Zhang ◽

...

Keyword(s):

Ovis Aries ◽

Domestic Sheep ◽

Sarcocystis Species ◽

Ovis Ammon

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ИДЕНТИФИКАЦИЯ МЕЖВИДОВЫХ ГИБРИДОВ АРХАРА (Ovis ammon) И ДОМАШНЕЙ ОВЦЫ (Ovis aries) РАЗНЫХ ПОКОЛЕНИЙ ПО ПОКАЗАТЕЛЯМ ЭКСТЕРЬЕРА

Sel skokhozyaistvennaya Biologiya ◽

10.15389/agrobiology.2020.6.1139rus ◽

2020 ◽

Vol 55 (6) ◽

pp. 1139-1147

Author(s):

Б.С. ИОЛЧИЕВ ◽

Keyword(s):

Ovis Aries ◽

Ovis Ammon

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Unveiling a wildlife haven: status and distribution of four Trans-Himalayan ungulates in Sikkim, India

Oryx ◽

10.1017/s0030605310000293 ◽

2010 ◽

Vol 44 (3) ◽

pp. 366-375 ◽

Cited By ~ 5

Author(s):

Pranav Chanchani ◽

Gopal S. Rawat ◽

Surendra P. Goyal

Keyword(s):

Small Region ◽

Ovis Aries ◽

Domestic Sheep ◽

Anthropogenic Pressure ◽

Small Populations ◽

Wild Ungulates ◽

Northern Boundary ◽

The Status ◽

Local Residents ◽

Ovis Ammon

AbstractWe conducted surveys in the Trans-Himalayan Tso Lhamo plateau in Sikkim, India, to ascertain the status and distribution of some of India’s last surviving populations of wild ungulates (numbers in brackets are estimates for Sikkim): Tibetan argali Ovis ammon hodgsoni (c. 177 individuals), Tibetan gazelle Procapra picticaudata (c. 77), and kiang Equus kiang polyodon (c. 18). Topography appears to have an important influence on ungulate distribution, and populations of these species are aggregated in a small region along the northern boundary of the plateau. Persistence of the wild ungulates in this area can be attributed to the non-hunting tradition of local residents and to the seasonal absence of livestock (yak Bos grunniens and domestic sheep Ovis aries) and grazing. Nonetheless, wild ungulates remain vulnerable because of their small populations and anthropogenic pressure on their habitats. We recommend that conservation be prioritized in important wildlife habitats through partnerships with local communities.

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28 DOUBLING OOCYTE CYTOPLASM VOLUME INCREASES BLASTOCYST QUALITY FOLLOWING INTERSPECIES SOMATIC CELL NUCLEAR TRANSFER IN ARGALI SHEEP (OVIS AMMON)

Reproduction Fertility and Development ◽

10.1071/rdv28n2ab28 ◽

2016 ◽

Vol 28 (2) ◽

pp. 144

Author(s):

A. L. Green ◽

F. C. Oback ◽

J. E. Oliver ◽

L. Popovic ◽

L. T. McGowan ◽

...

Keyword(s):

Somatic Cell ◽

Embryo Development ◽

Single Cells ◽

Statistical Significance ◽

Ovis Aries ◽

Domestic Sheep ◽

Custom Made ◽

Blastocyst Quality ◽

Ovis Ammon

Interspecies somatic cell NT (SCNT) can be used in the conservation of endangered animals but only when there is an abundant source of compatible oocytes and recipients. The objective of this study was to compare the efficiency of intra- and interspecies SCNT in sheep using zona-free embryo reconstruction methods. Skin fibroblasts from either an argali (Ovis ammon) or control (Ovis aries) ram were used as donor cells for SCNT between passages 2 to 5 and following culture in medium containing 0.5% FCS for 4 to 6 days. Single cells were electrically fused to cytoplasts prepared following enucleation of in vitro-matured zona-free metaphase II-arrested oocytes obtained from domestic ewes. In an additional experiment with argali, a double cytoplast (DC-SCNT) procedure was used whereby a second cytoplast was fused to the first reconstruct within 1 h. Reconstructs were artificially activated ~25 h after the start of maturation using ionomycin and 6-DMAP. Zona-free parthenogenote (PG) control oocytes were activated around the same time. In each treatment, 10 to 12 zona-free embryos where cultured in microwells formed in 20-μL drops of modified synthetic oviduct fluid under oil. Half the medium was replaced on Day 3, and developing embryos were transferred to individual 5-μL drops on Day 6. Development on Day 7 was expressed as a percentage of cleaved embryos. Statistical significance was determined using Fisher’s exact test for embryo development and two-tailed t-test for embryo cell numbers. Total embryo development on Day 7 was significantly greater with intraspecies sheep SCNT compared with interspecies argali SCNT (34/157 = 21.7% v. 34/363 = 9.4%, respectively; P < 0.001). Moreover, SCNT embryo development was significantly less than PG controls (122/360 = 33.9%; P < 0.001). Although argali DC-SCNT had no effect on total embryo development compared with SCNT (9/69 = 13.0% v. 7/56 = 12.5%, respectively), doubling cytoplasm volume increased the proportion of grade 1 and 2 embryos on Day 7 (8.7 v. 0%; P < 0.05). Consequently, DC-SCNT blastocysts had greater numbers of nuclei compared with SCNT (108 ± 47 v. 65 ± 9; n = 6, n = 6, respectively; P = 0.054). In comparison, PG blastocysts possessed on average 122 ± 27 nuclei (n = 7). A sample of 14 argali cloned blastocysts were all confirmed to have been derived from the respective ram after genotyping ~6000 ovine single nucleotide polymorphisms on a custom-made chip (Illumina, San Diego, CA, USA). Chromosome spreads of argali embryos revealed a modal number of 4 bi-armed autosomes as opposed to 6 in Ovis aries. In conclusion, in vitro development following interspecies SCNT in argali was about half that compared with domestic sheep. Blastocyst quality was improved by increasing oocyte cytoplasmic volume facilitated by zona-free cloning. Alternative sources of cytoplasm may further improve development. Confirmation that Ovis aries cytoplasm can fully reprogram a differentiated argali nucleus remains to be determined. This research was supported by AgResearch Core Funding.

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Infection levels of protostrongylid nematodes in definitive caprine and intermediate gastropod hosts from Uzbekistan

Journal of Helminthology ◽

10.1017/s0022149x16000171 ◽

2016 ◽

Vol 91 (2) ◽

pp. 236-243 ◽

Cited By ~ 3

Author(s):

A.E. Kuchboev ◽

J. Krücken ◽

R.R. Karimova ◽

B.H. Ruziev ◽

A. Pazilov

Keyword(s):

Morphological Analysis ◽

Small Ruminants ◽

Infection Intensity ◽

Ovis Aries ◽

Capra Hircus ◽

Intermediate Hosts ◽

Terrestrial Gastropods ◽

Infective Larvae ◽

Ovis Ammon

AbstractMorphological analysis of lungworms collected among Caprinae from Uzbekistan resulted in the identification of four species of Protostrongylidae: Protostrongylus rufescens, Protostrongylus hobmaieri, Spiculocaulus leuckarti and Cystocaulus ocreatus. The following species were recorded as definitive hosts: Ovis aries, Ovis ammon, Ovis vignei, Capra hircus, Capra falconeri and Capra sibirica. The prevalence of P. rufescens reached 45.3%, followed by S. leuckarti and C. ocreatus with 31.7% and P. hobmaieri with 16.9%. The sex ratio ranged between 1:3.1 and 1:6.2, with P. hobmaieri showing the strongest predominance of females over males. The prevalence of infection of small ruminants with protostrongylid nematodes increased with the age of the hosts. Protostrongyles use terrestrial gastropods as intermediate hosts, and infective larvae were found in the species Vallonia costata, Gibbulinopsis signata, Pupilla muscorum, Pseudonapaeus albiplicata, Pseudonapaeus sogdiana, Leucozonella ferghanica, Xeropicta candacharica, Candaharia levanderi and Macrochlamys sogdiana. Xeropicta candacharica was the most abundant gastropod and had the highest prevalence of infection with protostrongylids. Adult X. candacharica had a significantly higher infection intensity than juveniles. The epidemiology of protostrongylid infections is dynamic and subject to considerable changes. Further characterization of the interaction of protostrongylid parasites with their terrestrial gastropods as intermediate hosts and Caprinae as definitive hosts is required to understand these processes and to monitor the effects of changing ecological contexts.

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