scholarly journals Efficiency of spring barley haploid production in anther culture in vitro: comparison of basic and improved technologies

2019 ◽  
Vol 25 ◽  
pp. 178-183
Author(s):  
O. V. Bilinska ◽  
P. G. Dulnyev
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Spring Barley ◽  
Gelling Agent ◽  
Improved Method ◽  
Cold Pretreatment ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Modified Starches

Aim. Evaluation of innovative methodological approaches elaborated on model genotypes for usability to increase frequencies of morphogenic structure induction and plant regeneration in anther culture in vitro in spring barley diverse material. Methods. Spikes isolated from F1 and F2 hybrids of four crosses were pretreated using an improved method (4ºC, 28 days), and anthers were inoculated onto nutrient media containing chemically modified starches D5-M and D5a-1 instead of agar. In control cut tillers were emerged in water and pretreated at 4ºC for 5 days. Anthers were cultivated on agar solidified medium. Results. Positive effects of the improved method of cold pretreatment and cultivation of anthers on media solidified with starches were confirmed. The advantage of new gelling agent D5a-1 was proved. Particularly, its usage resulted in a three-fold increase in the frequency of green plant regeneration. Conclusions. In order to increase spring barley androgenic haploid yield, combination of prolonged cold pretreatment with anther cultivation on media solidified with chemically modified starches instead of agar in an integrated technological process is reasonable. Keywords: Hordeum vulgare L., anther culture in vitro, cold pretreatment, starch, agar, embryo formation, plant regeneration.

scholarly journals Effect of trophic, osmotically active and solidifying components of nutrient medium on the direct embryognesis in spring barley anther culture in vitro

2018 ◽  
Vol 22 ◽  
pp. 210-215
Author(s):  
E. V. Belinskaya ◽  
P. G. Dulnyev
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Spring Barley ◽  
Gelling Agent ◽  
Modified Starch ◽  
Hordeum Vulgare L ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Determine Mechanism

Aim. Ambryoidogenesis, or somatic embryogenesis, is known to be the most efficient mode of plant regeneration in plant cell, tissue and organ culture. The investigation was aimed to elucidate effects of trophic and osmogenic components of inductive medium on the frequency of direct embryoidogenesis in spring barley anther culture in vitro and to determine mechanism of morphogenesis improvement coursed by chemically modified starch D-5aM used as a gelling agent instead of agar. Methods. Anthers of DH-line with a high androgenetic capacity were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose or mannitol (0.3 M) and solidified with agar or chemically modified starch. Results. A positive effect of combination of high maltose content and chemically modified starch on the induction and regeneration processes in spring barley anther culture in vitro was confirmed. It was also shown that mannitol didn’t keep any growth or development processes going in barley anther and embryo culture, but at the same time this substance had no toxic effect. Conclusions. In order to achieve a high frequency of induction in spring barley anther culture, it is necessary to use medium containing maltose – a low weight component both with trophic and with osmotic activity. Keywords: Hordeum vulgare L., anther culture in vitro, mannitol, maltose, embryo formation, plant regeneration.

scholarly journals Grain barley starch as a gelling agent of nutrient media for cultivation of anthers and isolated embryos of H. vulgare L.

2020 ◽  
Vol 26 ◽  
pp. 158-163
Author(s):  
O. V. Bilynska
Keyword(s):  
Embryo Culture ◽  
Spring Barley ◽  
Gelling Agent ◽  
Modified Starch ◽  
Normal Type ◽  
Nutrient Media ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Pea Starch

Aim. Determination the possibility of agar replacement with normal and waxy type starches produced from barley seeds in the nutrient media for spring barley haploid production. Methods. Anthers of spring barley line DH00-126 were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose (9.0 %) and differed in solidifying agents (agar, starch from barley seeds or chemically modified starch D5a-1). Trophic capacities of barley starch were determined both in anther and embryo culture of barley. Results. It was determined that barley starch of waxy type was not suitable for solidifying of nutrient medium. Use of normal type barley starch (6.5 %) resulted in sufficient decrease in the frequency of green plant regeneration. At the same time, this modification had no effect on the number of morphogenic anthers. Barley starch was inferior to pea starch in the ability to support seedling growth in embryo culture in vitro. Conclusions. Barley starch of normal type has worse trophic capacities in comparison to pea starch in embryo culture. It was appeared barley starch to be a less suitable gelling agent of medium for spring barley haploid production in anther culture in vitro than agar and chemically modified starch D5a-1. Keywords: Hordeum vulgare L., anther culture in vitro, embryo culture in vitro, agar, barley starch.

scholarly journals Effect of low molecular weigt osmogenic substanses on the induction of embryogenesis in spring barley anther culture in vitro

1970 ◽  
pp. 164-167
Author(s):  
O. V. Bilynska
Keyword(s):  
Plant Regeneration ◽  
Osmotic Pressure ◽  
Anther Culture ◽  
Spring Barley ◽  
Hordeum Vulgare L ◽  
Culture In Vitro ◽  
Negative Effect ◽  
Embryoid Formation ◽  
The Impact

Aim. Osmotic pressure of nutrient medium is known to be an important factor for morphogenesis in plant cell, tissue and organ culture in vitro. The investigations was aimed to elucidate the effect of maltose concentration and to evaluate the impact of mannitol add on callus, embryoid formation and plant regeneration in spring barley anther culture in vitro. Methods. Anthers of DH-line with a high androgenetic capacity were inoculated on inductive media contained N6 macro-, MS micronutrients, organic supplements, maltose (0−9.0 %) and 0.1 M mannitol. Results. A decrease in maltose concentration from 9 to 6 % had a strong negative effect on these processes. At the same time, addition of 0.1 M mannitol to medium containing 6 % maltose promoted sufficiently increase the efficiency of embryoid formation and plant regeneration. Conclusions. Medium osmotic pressure mainly determined by a high maltose concentration played an important role in spring barley haploid production. Decrease in maltose content could be compensated by addition of mannitol, which is osmotic substance with low metabolic activity. Keywords: Hordeum vulgare L., anther culture in vitro, mannitol, embryo formation, plant regeneration.

scholarly journals Effect of medium gelatinized component on the effectiveness of androgenesis in vitro Oryza sativa L.

2016 ◽  
Vol 14 (2) ◽  
pp. 178-180
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
D. V. Shpak ◽  
A. O. Dobrova ◽  
S. O. Ignatova
Keyword(s):  
Anther Culture ◽  
Culture Medium ◽  
Oryza Sativa L ◽  
Modified Starch ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Androgenesis In Vitro ◽  
Negative Effect ◽  
Callus Regeneration

Aims. To study the effect of chemically modified starch D–5aM in the culture medium on the efficiency of androgenesis in vitro in anther culture of rice. Methods. Obtaining of rice double haploid lines by anther culture in vitro. The statistical methods. Results The influence different variants of gellatyne source in culture medium on the processes of induction and regeneration in anther culture of rice were studied. The 119 green plants-regenerants were received. Conclusions. The negative effect on the formation of green regenerants using a gel-forming components of the chemically modified starch D–5aM was shown. Keywords: rice, anther culture in vitro, callus, regeneration, chemically modified starch.

scholarly journals In vitro shoot regeneration through anther culture of Brassica spp.

1970 ◽  
Vol 35 (2) ◽  
pp. 331-341 ◽  
Author(s):  
MA Sayem ◽  
M Maniruzzaman ◽  
SS Siddique ◽  
M Al-Amin
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Maximum Rate ◽  
Media Composition ◽  
Shoot Initiation ◽  
The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone  DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

scholarly journals Pengaruh Praperlakuan Dingin Antera terhadap Pembentukan Kalus Cabai Merah Keriting (Capsicum annuum L.) Pretreatment on the Callus Formation of Curly Red Chilli Pepper (Capsicum annuum L.)

2020 ◽  
Vol 4 (2) ◽  
pp. 94-105
Author(s):  
Nida Wafiqah Nabila M Solin ◽  
Dian Adriani ◽  
Zulfahmi Zulfahmi ◽  
Mokhamad Irfan ◽  
Rosmaina Rosmaina
Keyword(s):  
Anther Culture ◽  
Capsicum Annuum ◽  
Callus Formation ◽  
Chili Pepper ◽  
Haploid Plant ◽  
Cold Pretreatment ◽  
Compact Structure ◽  
Chilli Pepper ◽  
Capsicum Annuum L

The production of the double haploid plant in vitro through anther culture technique is a plant breeding technique used to obtain pure strain rapidly. A variety of pretreatment has been reported to induce callus and regenerate planlets efficiently. This study aims at describing the influence of cold anther pretreatment towards the callus formation of curly red chili pepper (Capsicum annuum L.). This research was conducted in the laboratory of Genetics and Breeding, Faculty of Agriculture and Animal Science, Universtas Islam Negeri Sultan Syarif Kasim Riau. The explants used are anther of local genotype of curly red chili pepper. The anthers are stored at low temperatures (4 °c) with different time intervals of 0, 24, 48 and 72 hours. The results showed that the percentage of highest callus formation was obtained at 24 and 72 hours length storage, ie 50%. Cold pretreatment of 72 hours anther storage results in a faster callus with a percentage of the highest yellowish white callus color of 17.65% and a compact structure. The cold pretreatment with 72 hours anther storage is the most optimal acceleration in the development stage of anther culture and induces te formation of curly red chili pepper (Capsicum annuum L.) local genotypes.

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