scholarly journals КРУПНОЗЕРНЫЙ СОРТ ПЫРЕЯ СИЗОГО (Thinopyrum intermedium) СОВА КАК АЛЬТЕРНАТИВА МНОГОЛЕТНЕЙ ПШЕНИЦЕ

2021 ◽  
Vol 56 (3) ◽  
pp. 450-464
Author(s):  
В.П. ШAМAНИН ◽  
Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1114-1121 ◽  
Author(s):  
Shu-Mei Jiang ◽  
Long Zhang ◽  
Jun Hu ◽  
Rui Shi ◽  
Guang-He Zhou ◽  
...  

The alien addition line TAI-27 contains a pair of chromosomes of Thinopyrum intermedium that carry resistance against barley yellow dwarf virus (BYDV). A subtractive library was constructed using the leaves of TAI-27, which were infected by Schizaphis graminum carrying the GAV strain of BYDV, and the control at the three-leaf stage. Nine differentially expressed genes were identified from 100 randomly picked clones and sequenced. Two of the nine clones were highly homologous with known genes. Of the remaining seven cDNA clones, five clones matched with known expressed sequence tag (EST) sequences from wheat and (or) barley whereas the other two clones were unknown. Five of the nine differentially expressed sequences (WTJ9, WTJ11, WTJ15, WTJ19, and WTJ32) were highly homologous (identities >94%) with ESTs from wheat or barley challenged with pathogens. These five sequences and another one (WTJ18) were also highly homologous (identities >86%) with abiotic stress induced ESTs in wheat or barley. Reverse Northern hybridization showed that seven of the nine differentially expressed cDNA sequences hybridized with cDNA of T. intermedium infected by BYDV. Three of these also hybridized with cDNA of line 3B-2 (a parent of TAI-27) infected by BYDV. The alien chromosome in TAI-27 was microdissected. The second round linker adaptor mediated PCR products of the alien chromosomal DNA were labeled with digoxygenin and used as the probe to hybridize with the nine differentially expressed genes. The analysis showed that seven differentially expressed genes were homologous with the alien chromosome of TAI-27. These seven differentially expressed sequences could be used as ESTs of the alien chromosome of TAI-27. This research laid the foundation for screening and cloning of new specific functional genes conferring resistance to BYDV and probably other pathogens.Key words: suppression subtractive hybridization (SSH), expressed sequence tag (EST), linker adaptor mediated polymerase chain reaction (LA-PCR), chromosome microdissection.


2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Enrico Banfi

Elymus s. l. is a critical topic on which only a little light has begun to be made regarding phylogenetic reticulation, genome evolution and consistency of genera. In Italy, Elymus s. l. officially includes ten species (nine native, one alien) and some well-established and widespread hybrids generally not treated as little or nothing is known of them. In this paper fourteen species (with two subspecies) and six hybrids are taken into account and the following seven new combinations are proposed: Thinopyrum acutum (DC.) Banfi, Thinopyrum corsicum (Hack.) Banfi, Thinopyrum intermedium (Host) Barkworth and Dewey subsp. pouzolzii (Godr.) Banfi, Thinopyrum obtusiflorum (DC.) Banfi, Thinopyrum ×duvalii (Loret) Banfi, ×Thinoelymus drucei (Stace) Banfi, ×Thinoelymus mucronatus (Opiz) Banfi. Some observations are provided for each subject and a key to species, subspecies and hybrids is made available.


Author(s):  
B.J. Wills ◽  
G.B. Douglas ◽  
J. Mckenzie ◽  
K.D. Trainor ◽  
A.G. Foote

Intermediate and pubescent wheatgrasses (Thinopyrum intermedium) are potentially useful as dryland forage grasses in New Zealand. The recent literature on their international and local use in pasture and cropping situations is reviewed. Under semi-arid conditions at Bendigo Station over a 13-year period, cv. Luna wheatgrass has spread several metres from the original drill lines and it provides very high ground cover. In terms of dryland soil resource conservation this contrasts with adjacent cocksfoot and, to a lesser extent, lucerne plots which have wide-spaced plants interspersed with litter and much bare ground. In a mixed drilling containing wheatgrass, hairy dorycnium, birdsfoot trefoil and sheep's burnet, the first two species now dominate. At Takapau, central Hawke's Bay, annual yields from intermediate and pubescent wheatgrasses produced over 80% of the yield of 10 New Zealand cultivars in spring, summer and autumn, and 65% in the winter. Of the wheatgrasses, cv. Topar intermediate wheatgrass and a pubescent wheatgrass (unnamed line) were the lowest yielding, whereas cv. Oahe and cv. Mandan produced the highest yields. Cv. Greenleaf pubescent wheatgrass had higher foliar nitrogen content than all other species, but in vitro organic matter digestibility was less than 700 g OM/kg DM. Although the wheatgrasses had satisfactory herbage yield and nutritional value, better alternative species are available for pastoral farming in central Hawke's Bay. On Belfield in the Hakataramea Valley, cv. Rush has been the most vigorous wheatgrass for establishment and it seems to handle cold winds and frost well. Hay made from a wheatgrass/ lucerne mix is considered better than that from the individual species as this can increase production by 40-50%, depending on soil and climatic conditions. Usually only one cut per year is taken from the mixed pasture. Crop maintenance includes the application of 200 kg/ha of sulphur super extra every 3-4 years and the pH is maintained at about 5.9-6.4 by liming. A significant advantage is that no stock health, pest or plant disease problems have been noted as yet with any of the wheatgrass varieties available in New Zealand. Keywords: dryland agriculture, New Zealand, Thinopyrum, wheatgrass


Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 385-394 ◽  
Author(s):  
P. J. Larkin ◽  
P. M. Banks ◽  
E. S. Lagudah ◽  
R. Appels ◽  
Chen Xiao ◽  
...  

Zhong 5 is a partial amphiploid (2n = 56) between Triticum aestivum (2n = 42) and Thinopyrum intermedium (2n = 42) carrying all the chromosomes of wheat and seven pairs of chromosomes from Th. intermedium. Following further backcrossing to wheat, six independent stable 2n = 44 lines were obtained representing 4 disomic chromosome addition lines. One chromosome confers barley yellow dwarf virus (BYDV) resistance, whereas two other chromosomes carry leaf and stem rust resistance; one of the latter also confers stripe rust resistance. Using RFLP and isozyme markers we have shown that the extra chromosome in the Zhong 5-derived BYDV resistant disomic addition lines (Z1, Z2, or Z6) belongs to the homoeologous group 2. It therefore carries a different locus to the BYDV resistant group 7 addition, L1, described previously. The leaf, stem, and stripe rust resistant line (Z4) carries an added group 7 chromosome. The line Z3 has neither BYDV nor rust resistance, is not a group 2 or group 7 addition, and is probably a group 1 addition. The line Z5 is leaf and stem rust resistant, is not stripe rust resistant, and its homoeology remains unknown.Key words: Agropyron, intermediate wheatgrass, leaf rust, stem rust, stripe rust, luteovirus.


Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 490-495 ◽  
Author(s):  
F P Han ◽  
G Fedak ◽  
A Benabdelmouna ◽  
K Armstrong ◽  
T Ouellet

Restriction fragment length polymorphism (RFLP) analysis and multicolor genomic in situ hybridization (GISH) are useful tools to precisely characterize genetic stocks derived from crosses of wheat (Triticum aestivum) with Thinopyrum intermedium and Thinopyrum elongatum. The wheat × Th. intermedium derived stocks designated Z1, Z2, Z3, Z4, Z5, and Z6 were initially screened by multicolor GISH using Aegilops speltoides genomic DNA for blocking and various combinations of genomic DNA from Th. intermedium, Triticum urartu, and Aegilops tauschii for probes. The probing (GISH) results indicated that lines Z1 and Z3 were alien disomic addition lines with chromosome numbers of 2n = 44. Z2 was a substitution line in which chromosome 2D was substituted by a pair of Th. intermedium chromosomes; this was confirmed by RFLP and muticolour GISH. Z4 (2n = 44) contained two pairs of wheat – Th. intermedium translocated chromosomes; one pair involved A-genome chromosomes, the other involved D- and A-genome chromosomes. Z5 (2n = 44) contained one pair of wheat – Th. intermedium translocated chromosomes involving the D- and A-genome chromosomes of wheat. Z6 (2n = 44) contained one pair of chromosomes derived from Th. intermedium plus another pair of translocated chromosomes involving B-genome chromosomes of wheat. Line Z2 was of special interest because it has some resistance to infection by Fusarium graminearum.Key words: wheat, Thinopyrum intermedium, addition, substitution, and translocation lines, GISH, multicolor GISH, RFLP.


2019 ◽  
Vol 96 (5) ◽  
pp. 927-936
Author(s):  
Yingxin Zhong ◽  
Juan Mogoginta ◽  
Joseph Gayin ◽  
George Amponsah Annor

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