Specificities of changes in the concentrations of heavy metals in milk thistle (Silybum marianum (L.) Gaertn.)

2015 ◽  
Vol 2 (3) ◽  
pp. 55-60
Author(s):  
O. Rudnyk-Ivashchenko ◽  
L. Mykhal’ska ◽  
V. Schwartau

Aim. To investigate the content of heavy metals in the seeds of milk thistle (Silybum marianum (L.) Gaertn.) and in the product of its processing – oil. To reveal possible changes in the concentration of heavy metals in the years of cultivating this plant. Methods. The fi eld and spectrometric methods were applied; the results were processed using the technologies, common for agriculture, plant production and statistics. Results. The content of heavy metals in the seeds of milk thistle was defi ned. It was determined that their concentration exceeds maximal acceptable coeffi cient for the medicinal plant material. It was established that in 2013 the seeds of milk thistle accumulated several times more Ba, Ca, Fe, Mg, Mo, Sr, V compared to the indices of 2014, while Al, Co, Ag were found only in the samples of the seeds, harvested in 2013. Conclusions. As the regulatory documents regarding many relevant elements, which belong to heavy metals, do not specify maximal accept- able coeffi cients for the content of the latter in the medicinal plant material, it is reasonable to conduct complex studies, involving the specialists of relevant fi elds, in order to stipulate this gradation. In the Kyiv region the milk thistle plants accumulate a large amount of such heavy metals as cadmium, manganese, strontium, and zinc, which exceeds maximal acceptable degrees of accumulation and testifi es to partial pollution of region soils. It was established that the concentration of the main part of heavy metals in the product of processing milk thistle – oil – is decreased to the value under the threshold of analytic devices. This fact proves the pros- pects of growing milk thistle for industrial processing purposes.

2016 ◽  
Vol 3 (3) ◽  
pp. 66-72
Author(s):  
O. Rudnyk-Ivashchenko ◽  
O. Yaruta

Aim. To investigate the content of heavy metals (HM) in the seeds and roots of Atropa belladonna plants. To identify possible changes in their concentrations over the years of growing these plants. Methods. Field and spectrometric method, methods, common for agriculture, plant growing and statistics. Results. The content of heavy metals (HM) in the seeds and roots of belladonna plants was determined. It was found that plant roots ac- cumulate them much more compared to the seeds. The concentration of such HM as barium, cadmium, copper, iron, manganese, nickel, potassium, sodium, strontium, and zinc in the plants exceeds the maximum permis- sible level, which testifi es to partial soil contamination in the region. Conclusions. As there are no data about maximum permissible ratios for the content of important chemical elements in the raw plant material, specifi ed in normative and regulatory documents, scientists have to launch complex investigations, involving specialists of relevant fi elds to identify the mentioned gradation. It was determined that the main part of HM is accumu- lated in the roots of belladonna plants, whereas their content in the seeds is several times smaller or equals the value under the sensitivity threshold of analytical devices. This fact testifi es to the prospects of growing the mentioned medicinal herb in Kyiv region for further industrial processing. Chemical elements, which belong to compounds of lanthanides and actinides, are almost not accumulated in belladonna plants.


Author(s):  
Sara Safikhan ◽  
Mohammad Reza Chaichi

Identifying environmental factors, plant characteristics, and agronomic activities plays an essential role in medicinal plant production. Milk thistle (Silybum marianum L.) is a well-known medicinal plant with extensive use in diverse liver diseases and is economically a significant crop. This research was conducted to evaluate the effect of the sole and integrated applications of graphene oxide (GO), zeolite, and chitosan as modifying materials on gas exchange and the secondary metabolites of milk thistle under severe salinity stress. Seven sole and integrated combinations of nano-materials comprised of T1, T2, T3, T4, T5, T6, T7, and control (T8, no nano-materials application) and two levels of saline water (12 ds/m) and tap water (control, 0.8 ds/m) were applied to the soil of experimental plots based on a factorial design with three replications. The results showed that the highest photosynthesis rate was obtained with T7 treatment for both water treatments. The highest plant silymarin concentration was obtained from the T6 treatment under both saline and tap water conditions. This treatment increased the silymarin concentration by 15.9% compared to the T8. The highest plant silymarin yield (180 mg per plant) was recorded for the T7 under tap water (control) condition, and 130.3 mg/plant for T6 under salinity stress, respectively. The Transmission Electron Microscope technology indicated that GO at low concentration (0.01%) could be safely used to enhance milk thistle germination and growth under severe salinity stress conditions


2019 ◽  
Vol 8 (4) ◽  
pp. 48-52
Author(s):  
O. V. Trineeva

Introduction. Recently, much attention has been paid to the primary assessment of the pharmacological effect of various drugs using in vivo and in vitro tests. It is known that such a medicinal plant as sea buckthorn, in its phytochemical composition is rich in natural antioxidants: carotenoids, tocopherols, flavonoids, ascorbic acid, etc. In some publications there is information about the antioxidant activity of sea buckthorn and fatty oil based on them. However, information on the comparative characteristics of the use of various methods for determining the antioxidant activity of this type of medicinal plant material and the results obtained are not found in the scientific literature.Aim. The aim of this work was a comparative determination of the antioxidant activity of medicinal plant material of buckthorn fruits of various species of buckthorn.Materials and methods. The total antioxidant activity of water and water-alcohol extracts from the fruits of sea buckthorn fruits was determined using various techniques recommended in the literature. The antioxidant activity of the extracts was determined by permanganometric titration, in vitro inhibition of adrenaline autooxidation, and also in a biological model, Parametium caudatum cell culture.Results and discussion. The effect of the extractant polarity on the value of antioxidant activity was studied. It was found that the highest content of antioxidants in the extraction is observed when using 96 % ethanol as an extractant.Conclusion. Using three methods, the prospects of using sea buckthorn fruits and preparations based on them as a source of antioxidants are shown. 


2019 ◽  
pp. 25-38
Author(s):  
M. A. Dorri ◽  
B. Kamkar ◽  
M. Aghdasi ◽  
A. R. Safahani

Milk thistle (Silybum marianum) is used as a medicinal plant in the treatment of liver diseases. In order to investigate germination and seedling growth in S. marianum subjected to NaCl, a three replicated experiment was carried out in a randomized complete block design in the laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Treatments included seven salinity levels (50, 75, 100, 125, 150, 175 and 200 mM) and a control (distilled water). Germination percentage (GP), mean germination time (MGT), seed-ling vigour index (SVI), shoot, root and seedling weight were measured. The effect of salinity levels was significant on GP, SVI, MGT and seedling weight and length (p ≤ 0.01). However, the NaCl concentration effect was not significant on shoot : root length ratio and or shoot : root weight ratios. Results showed that germination decreased when salinity increased, while MGT increased. MGT was 1.75 times higher than in the control at the highest salt concentration. MGT difference was not significant between 75 mM NaCl and con-trol, while it dramatically increased by increasing the NaCl concentration from 150 to 200 mM NaCl. Reduc-tion slope and salt tolerance index (STI) were estimated for germination (0.54 and 231.9) and seedling stage (0.24 and 237.4). According to the results, milk thistle could be considered as a valuable medicinal plant in fairly salinized areas.


2001 ◽  
Vol 7 (3-4) ◽  
Author(s):  
G. Karimzedah ◽  
R. Omidbaigi ◽  
Bakhshai D.

Milk thistle (Silybum marianum (L.) Gaertn) has been used in medicine since ancient times, but it has been cultivated as a medicinal plant only in recent decades. The ripe fruit of milk thistle contains flavonoids, which are used to prepare anti-hepatotoxic drugs. The main purpose of this study was to substantiate the effects of irrigation and row spacing on growth, seed yield and the content of active substances (silybin and silymarin) in milk thistle. The results showed that the suitable amount of irrigation was 20 mm and the appropriate row spacing was 25 cm.  


2004 ◽  
Vol 38 (11) ◽  
pp. 614-617
Author(s):  
E. V. Ivanov ◽  
M. V. Shvyrev ◽  
S. A. Minina ◽  
V. G. Kochnev

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