LYMPHOCYTES IN PERIPHERAL BLOOD AND THYROID TISSUE IN CHILDREN WITH GRAVES' DISEASE

AbstractObjectivesGraves’ disease (GD) is an autoimmune disease involving intimate response of both T cells and B cells. Immunophenotyping of peripheral blood lymphocyte subsets in GD children with different clinical characteristics can provide further information of the pathogenesis of GD.MethodsWe studied the lymphocyte subsets in peripheral blood of 141 children with GD. We repeatedly divided the patients into two groups in accordance with different clinical characteristics (abnormal or normal alanine aminotransferase (ALT) levels, the presence or absence of Graves’ orbitopathy (GO), and the presence or absence of hematuria. Then we compared the lymphocyte subsets measurements between two paired groups.ResultsWe found that serum ALT levels correlated positively with CD3+CD8+ T cell percentages in children with GD. Moreover, we detected higher percentages of CD3−CD19+ cells and higher ratio of CD4/CD8 in patients with GO. However, no correlation was found between GO status and lymphocyte subsets after excluding confounding effect of TRAb. No difference of lymphocyte subset percentages was found between groups with or without hematuria.ConclusionsSerum ALT levels correlate positively with cytotoxic T cell percentages in the peripheral blood of children with GD. The cytotoxic T cell may play a role in the pathogenesis of hepatic dysfunction in children with GD.

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Lack of T-Cell Immune Abnormalities in Peripheral Blood Lymphocytes in Patients with Graves' Disease or Hypothyroidism

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem-41-2-266 ◽

1975 ◽

Vol 41 (2) ◽

pp. 266-270 ◽

Cited By ~ 20

Author(s):

CHILANGO MULAISHO ◽

NABIH I. ABDOU ◽

ROBERT D. UTIGER

Keyword(s):

T Cell ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Graves Disease ◽

Blood Lymphocytes

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Thyroid papillary carcinoma: preliminary evidence for a germ-line single nucleotide polymorphism in the Fas gene

Journal of Endocrinology ◽

10.1677/joe.0.1820479 ◽

2004 ◽

Vol 182 (3) ◽

pp. 479-484 ◽

Cited By ~ 13

Author(s):

F Basolo ◽

R Giannini ◽

P Faviana ◽

G Fontanini ◽

A Patricelli Malizia ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Germ Line ◽

Thyroid Tissue ◽

Preliminary Evidence ◽

Graves Disease ◽

Nucleotide Polymorphism ◽

Single Strand Conformational Polymorphism ◽

Single Nucleotide ◽

N 93 ◽

Immunohistochemical Studies

The expression of Fas in thyroid tumours and Graves' disease was analysed by mRNA transcript expression. As compared with unaffected thyroid tissue, Fas expression was enhanced in Graves' disease, adenomas, and papillary and follicular carcinomas. This pattern was also reflected in immunohistochemical studies. The PCR single-strand conformational polymorphism (SSCP) method and DNA sequencing were used to analyse Fas exons 1-9. The study was carried out on five different histotypes of thyroid tumours (n=93) and tissue from Graves' disease patients. As compared with a group of healthy blood donors (n=64), a significant association (P=0.006) emerged between papillary thyroid carcinoma and a silent single nucleotide polymorphism (SNP, 988C-->T) in exon 7 of the Fas gene. Other forms of thyroid pathology were not associated with the above polymorphism. Patients with neoplasia showed the same SNP in tumour tissue, in the unaffected contralateral thyroid lobe, and in peripheral blood cells. Thus, the 988C-->T polymorphism appeared to be of germ-line origin.

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Toll like receptor 9 (TLR 9) expression in peripheral blood mononuclear cell (PBMCs) from treated and untreated Grave’s disease patients

QJM ◽

10.1093/qjmed/hcab100.083 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Ashraf M Okba ◽

Rasha Y Shaheen ◽

Gehan M. H Mostafa ◽

Hanan M Ali ◽

Sylvia W Abo El Fadle ◽

...

Keyword(s):

Peripheral Blood Mononuclear Cell ◽

Mononuclear Cell ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Lymphocytes ◽

Graves Disease ◽

Toll Like Receptor ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

Abstract Background It is well known that Autoimmune thyroid disease is multifactorial with multiple genetic and environmental factors, immune malfunction also incriminated in the development of this disease, The exact pathogenesis of this disease remains unclear despite the fact that the production of autoantibodies destroys self-tolerance and agitate the adaptive immune system. Our study will answer the question is there a difference in Toll like receptor 9 (TLR 9) expression in peripheral blood mononuclear cell (PBMCs) from Grave’s disease patients. Objective to measure TLR9 percentage expression on peripheral blood mononuclear cells in patients with Graves’ disease. Methods 60 subjects were included in this study; 30 with Graves’ disease and 30 healthy individuals as control group. All the patients were subjected to the following: Full history, clinical examination, thyroid functions, Thyroid ultrasound, Radioisotope thyroid scan: to assess uptake of thyroid gland and Toll like receptor 9 (TLR 9) percentage expression on peripheral blood mononuclear cells will be analyzed using flow cytometry technique. Results The present study proved that patients with Graves’ disease had higher levels of percentage expression of TLR 9 on peripheral blood lymphocytes. Conclusion percentage expression of TLR9 on peripheral blood lymphocytes is higher in Graves’ patients.

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