scholarly journals Changes in erythrocytopoesis indices in dogs with babesiosis

2018 ◽  
Vol 9 (3) ◽  
pp. 379-383 ◽  
Author(s):  
V. I. Holovakha ◽  
О. V. Piddubnуak ◽  
T. I. Bakhur ◽  
N. V. Vovkotrub ◽  
A. A. Antipov ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Age Groups ◽  
Acid Resistance ◽  
Hypochromic Anemia ◽  
Common Disease ◽  
Babesia Canis ◽  
Age Composition ◽  
Hemoglobin Molecule ◽  
Total Tissue

Babesiosis is a common disease in dogs. Babesia canis (Piana & Galli-Valerio, 1895) (Sporozoa, Babesiidae) causes the destruction of erythrocytes, resulting in hypotensive shock and total tissue damage due to lack of oxygen. Because of babesiosis, anemia develops in dogs, and in the first hours of the disease it is normocytic, normochromic and nonregenerative, and on the 2–3rd day of the course, macrocytic, hypochromic anemia with reticulocytosis develops. Scientists have studied the most common indicators of evaluation of erythrocytopoesis during babesiosis (the number of red blood cells, hemoglobin, hematocrit index, indices of “red” blood MCH and MCV), but the age structure of erythrocytes, their acid resistance and the ferrum-transferrin complex for this parasitic pathology have not been sufficiently studied. We carried out research on dogs of service breeds, which were divided into two groups: the first (6–18 months old, n = 10) and the second (2–8 years, n = 15). According to the conducted studies, in dogs of different age groups with babesiosis revealed oligocythemia with anisocytosis and poikilocytosis, oligochromia, decreased hematocrit index and macrocytosis. Significant changes in the morpho-functional state of erythrocytes in dogs of both groups have been revealed, in particular, changes in the population (age) composition of red blood cells: the number of “old” erythrocytes increases (they are rapidly destroyed), the “young” forms of red blood cells decrease and the time for their hemolysis is reduced. As a result of the study of the ferrum-transferrin complex in dogs with babesiosis in both groups, an increase in the level of free ferrum (UIBC) and a decrease in the saturation of transferrin with the trace element was found, which makes it impossible to form a hemoglobin molecule in the bone marrow.

HENNA (Lawsonia inermis) A MEDICINAL HERB EFFECTIVE IN SICKLE CELL DISEASE

2021 ◽  
pp. 01-04
Author(s):  
KRISHNA KUMAR ◽  
Nitish Kumar ◽  
Amresh gupta ◽  
Arpita singh ◽  
Pandey Swarnima ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Blood Cells ◽  
Oxygen Affinity ◽  
Common Disease ◽  
Cell Disease ◽  
Lawsonia Inermis ◽  
Sodium Bisulphite ◽  
Sickle Cells

Sickle cell anemia is a common disease in Oman country. In this disease, sickle-shaped cells are formed. These cells interrupt blood vessels and cause a reduction in oxygen transportation. It was founded that henna (Lawsonia inermis) can prohibit the formation of sickle cells. The Lawsone (2-Hydroxy-1,4-Naphthoquinone) is the constituents of henna which is responsible for the anti-sickling activity, by increasing the oxygen affinity of red blood cells. Hena has the anti-sickling activity which is proved by incubating aqueous and methanolic henna extracts with sickle cell disease patient's whole blood. Then for reduction to oxygen tension 2%, sodium bisulphite was added. Therefore, the percentage of sickled cells to normal red blood cells was observed at 30 minutes intervals. Henna proved a delay in the sickling process in 84% of the tested samples. Both extracts(aqueous and methanolic henna) can delay sickling for about an hour.

scholarly journals ELECTRON MICROSCOPE ANALYSIS OF YOUNG AND OLD RED BLOOD CELLS STAINED WITH COLLOIDAL IRON FOR SURFACE CHARGE EVALUATION

1969 ◽  
Vol 43 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Y. Marikovsky ◽  
D. Danon
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Membrane Surface ◽  
Phthalate Esters ◽  
Age Groups ◽  
Red Cells ◽  
Electric Mobility ◽  
Iron Particles ◽  
Colloidal Iron ◽  
Thin Sections

Human and rabbit red blood cells, separated into "young" and "old" age groups by differential flotation on phthalate esters, were fixed with glutaraldehyde and labeled with colloidal ferric oxide. Electron micrographs of thin sections of young cells showed a uniform and dense depostion of positive iron particles. Old cells showed particles deposited irregularly, leaving unlabeled gaps on the membrane surface. Red cells incubated with 10 units/ml receptor-destroying enzyme (RDE) demonstrate a reduced labeling, similar to that of old cells. After neuraminic acid had been removed from red cells by 20 units/ml RDE, no iron particles were found on membrane surfaces. The different labeling of young, old, and RDE-treated human and rabbit red cells was correlated with their electric mobility and agglutinability by poly-L-lysine. The contradiction between the apparent similarity in charge density of human and rabbit red cells as estimated by density of iron particles and the markedly lower electric mobility of rabbit red cells is discussed.

scholarly journals High-throughput assessment of hemoglobin polymer in single red blood cells from sickle cell patients under controlled oxygen tension

2019 ◽  
Vol 116 (50) ◽  
pp. 25236-25242 ◽  
Author(s):  
Giuseppe Di Caprio ◽  
Ethan Schonbrun ◽  
Bronner P. Gonçalves ◽  
Jose M. Valdez ◽  
David K. Wood ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Oxygen Tension ◽  
Sickle Cell ◽  
Blood Cells ◽  
Severe Disease ◽  
Oxygen Affinity ◽  
Fetal Hemoglobin ◽  
Treatment Development ◽  
Hemoglobin Molecule ◽  
Transfer Strategies

Sickle cell disease (SCD) is caused by a variant hemoglobin molecule that polymerizes inside red blood cells (RBCs) in reduced oxygen tension. Treatment development has been slow for this typically severe disease, but there is current optimism for curative gene transfer strategies to induce expression of fetal hemoglobin or other nonsickling hemoglobin isoforms. All SCD morbidity and mortality arise directly or indirectly from polymer formation in individual RBCs. Identifying patients at highest risk of complications and treatment candidates with the greatest curative potential therefore requires determining the amount of polymer in individual RBCs under controlled oxygen. Here, we report a semiquantitative measurement of hemoglobin polymer in single RBCs as a function of oxygen. The method takes advantage of the reduced oxygen affinity of hemoglobin polymer to infer polymer content for thousands of RBCs from their overall oxygen saturation. The method enables approaches for SCD treatment development and precision medicine.

Rainbow trout (Oncorhynchus mykiss) shift the age composition of circulating red blood cells towards a younger cohort when exposed to thermal stress

2012 ◽  
Vol 182 (5) ◽  
pp. 663-671 ◽  
Author(s):  
Johanne M. Lewis ◽  
Georgia Klein ◽  
Patrick J. Walsh ◽  
Suzanne Currie
Keyword(s):  
Thermal Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Age Composition ◽  
Rainbow Trout Oncorhynchus Mykiss

Differences in blood values depending on age in laboratory-bred european hamsters (Cricetus cricetus L.)

1975 ◽  
Vol 9 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Adelheid Emminger ◽  
G. Reznik ◽  
Hildegard Reznik-Schüller ◽  
U. Mohr
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Age Groups ◽  
Blood Parameters ◽  
Cricetus Cricetus ◽  
Males And Females

No significant differences in blood parameters were found between males and females aged 13-900 days. A comparison of the various age groups showed significant differences in the number of erythrocytes, leucocytes, thrombocytes and in the diameter of red blood cells. The number of erythrocytes and leucocytes was lowest, and the erythrocytes had the largest diameters in young animals. The number of thrombocytes was lowest in older animals.

scholarly journals Continuous changes in biological levels of complete blood count in a high altitude area of China

2020 ◽  
Vol 8 ◽  
pp. 205031212093133 ◽  
Author(s):  
Xuan Luo ◽  
Lei Feng ◽  
XueJing Bai ◽  
JiangXian Zhu ◽  
GuanCheng Zhao ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Complete Blood Count ◽  
White Blood Cells ◽  
Age Groups ◽  
Reference Interval ◽  
Distribution Width ◽  
Men And Women ◽  
Cell Parameters

Objectives: We aimed to establish a new reference interval of blood cell parameters by classifying and counting blood Cells of 16- to 85-year-old healthy volunteers and observing continuous changes with age. Methods: We analyzed the blood cell parameters of 42,678 cases (men, 24,406; women, 18,272), and compared the blood cell parameters of men and women in different age groups using an independent samples t-test. Using limits of 2.5%–97.5%, a 90% confidence interval was used to develop new reference intervals. Results: Counts of blood cell parameters, including white blood Cells, neutrophils, lymphocytes, monocytes, eosinophils, basophils, red blood Cells, hemoglobin, hematocrit, distribution width of red blood Cells and platelets, were found to differ between men and women in different age groups. These parameters were used to establish a new reference interval of blood Cells. Conclusion: The blood cell parameters of both men and women changed with increasing age. The reference interval that we established will provide more accurate basic evidence for clinical diagnosis and treatment of diseases.

Interindividual variation in total and carrier-mediated lactate influx into red blood cells

1998 ◽  
Vol 274 (4) ◽  
pp. R1025-R1030 ◽  
Author(s):  
Leena K. Väihkönen ◽  
A. Reeta Pösö
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Venous Blood ◽  
Age Groups ◽  
Lactate Concentration ◽  
Bimodal Distribution ◽  
Monocarboxylate Transporter ◽  
Interindividual Variation ◽  
Transport Activity ◽  
Major Carrier

To study in standardbred horses interindividual variation in the influx of lactate into red blood cells, venous blood samples were collected from 89 horses from 2 wk to 9 yr of age. For 62 horses, the rate of influx was normally distributed with a mean rate of 4.09 nmol ⋅ mg protein−1 ⋅ min−1at a lactate concentration of 10 mM, and the respective value for the other 27 horses was 0.58 nmol ⋅ mg protein−1 ⋅ min−1. At 30 mM of lactate, the rates were 8.71 and 1.97 nmol ⋅ mg protein−1 ⋅ min−1, respectively. This bimodal distribution was independent of age. In horses with high transport activity, the monocarboxylate transporter (MCT) appears to be the major carrier, whereas, in those with low transport activity, no activity of the MCT could be detected. The band 3 protein may account for 18–39% of transport activity. With all age groups combined, the transport activity tended to be higher in mares than in stallions. Lactate transport into red blood cells seems thus to be an inherent property in which participation of various transporters varies interindividually.

Microthermal method of separating red blood cells into age groups

1977 ◽  
Vol 84 (3) ◽  
pp. 1372-1375
Author(s):  
N. A. Sizova ◽  
V. V. Kamenskaya ◽  
V. I. Fedenkov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Age Groups

scholarly journals ЗМІНИ ВЛАСТИВОСТЕЙ ЕРИТРОЦИТІВ У СОБАК

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 3-6
Author(s):  
M.V. Anforova ◽  
V.I. Golovakha ◽  
O.V. Piddubnyak ◽  
M.J. Tyshkivskyy
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid Membranes ◽  
Acid Resistance ◽  
Small Animals ◽  
Hemoglobin Content ◽  
Population Composition ◽  
Rapid Destruction ◽  
Healthy Dogs ◽  
Composition Changes

One of the most important indicators is the assessment of erythroсytopoezis is acid resistance of erythrocytes and their population composition. Changes in these parameters are the most studied in humans, cattle and horses. In small animals, including dogs, the issue is not highlighted enough. Therefore, the study of changes of erytroсytopoezis by using these tests is important. The study erythroсytopoezis parameters was performed in dogs of all ages (1.5–year, 3 – 6 years and 7 and older years of age). It was found that in clinically healthy dogs generall erythroсytopoezis indicators (total number of red blood cells, hemoglobin content, hematocrit value, the index «red» blood – МСН and МСV), regardless of age, did not significantly change. Yet in dogs older than 7 years there were found some changes on the the part of erythroсytopoezis. This was pointed by the changes of the population composition of red blood cells and their acid resistance. In particular, the animals showed an increased number of «old» populations of red blood cells and some changes of their hemolytic resistance. Particularly, the height of the erytrohram peak was 15% higher than in younger animals. The very peak was sharp, indicating the rapid destruction of the «young» red cells and degeneration of their lipid membranes. 

scholarly journals Mechanisms Involved in the Persistence of Babesia canis Infection in Dogs

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 94 ◽  
Author(s):  
Schetters
Keyword(s):  
Endothelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Infected Erythrocyte ◽  
Clinical Signs ◽  
Coagulation System ◽  
Blood Capillary ◽  
Babesia Canis ◽  
Large Numbers ◽  
Erythrocyte Surface

Dogs that are infected with Babesia canis parasites usually show severe clinical signs, yet often very few parasites are detectable in the blood circulation. The results showed that large numbers of B. canis-infected red blood cells accumulate in the microvasculature of infected subjects. The initial process leading to the attachment of infected erythrocytes to the endothelial cells of small capillaries (sequestration) appears to involve the interaction of parasite molecules at the erythrocyte surface with ligands on the endothelial cells. Since parasites continue to develop in the sequestered erythrocyte, it would be expected that the infected erythrocyte is destroyed when the mature parasites escape the host cell, which would make it hard to explain accumulation of infected erythrocytes at the initial site of attachment. Apparently, additional processes are triggered that lead to consolidation of parasite sequestration. One possible explanation is that after initial attachment of an infected erythrocyte to the wall of a blood capillary, the coagulation system is involved in the trapping of infected and uninfected erythrocytes. The data further suggest that newly formed parasites subsequently infect normal red blood cells that are also trapped in the capillary, which finally leads to capillaries that appear to be loaded with infected erythrocytes.

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