Unique report of two Sarcocystis species from Egyptian domestic chicken (Gallus gallus) – new host and locality

2021 ◽  
Vol 24 (1) ◽  
pp. 152-158
Author(s):  
E. M. Galila ◽  
E. K. A. Bazh ◽  
N. Elhawary ◽  
H. A. Abdellatif ◽  
A.-R. A. Abou-Rawash
Keyword(s):  
Nile Delta ◽  
Protozoan Parasite ◽  
Intestinal Wall ◽  
Sarcocystis Species ◽  
Middle Region ◽  
New Host ◽  
Sarcocystis Spp ◽  
Hexagonal Shape ◽  
The World ◽  
Intracellular Protozoan Parasite

Sarcocystis is an intracellular protozoan parasite in the phylum Apicomplexa. It is widely distributed all over the world. There are scarce reports about chicken Sarcocystis. From February 2016 to January 2018, a total number of 630 chicken carcasses, intestines and viscera were collected from different chicken markets in Menoufia and Gharbia Governorates, Middle region of the Nile Delta, Egypt and carefully inspected. Macroscopic and microscopic cysts of Sarcocystis spp. were found in the intestinal wall and mesentery of 5 birds. Histopathological sections revealed the presence of two shapes of the macroscopic cysts (oval and kidney shape). Their wall was striated and characterised by the presence of radial septa. It had compartments mostly of hexagonal shape, containing both bradyzoites and metrocytes in the periphery. The bradyzoites were banana-shaped and measured 20–30 × 8–10 μm with centrally or posteriorly located nuclei. Microscopic cysts of Sarcocystis spp. were detected in-between muscle bundles, with variable shapes (spindle and oval).

First molecular detection and characterization of Sarcocystis infection in naturally infected buffaloes, Brazil

2020 ◽  
Author(s):  
Luiza Pires Portella ◽  
Fagner D'ambroso Fernandes ◽  
Camila Encarnação Minuzzi ◽  
Juliana Felipetto Cargnelutti ◽  
Luis Fernando Vilani de Pelegrini ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Genomic Dna ◽  
Molecular Detection ◽  
Natural Infection ◽  
Sarcocystis Species ◽  
Sarcocystis Spp ◽  
The World ◽  
First Time ◽  
Pathogenic Effects

Sarcocystosis is a disease caused by varying Sarcocystis species infecting humans and animals. It is commonly found in ruminants causing pathogenic effects. Although the distribution of Sarcocystis can be found all over the world, the species infecting buffaloes in Brazil is still unknown. Through this study, we aim to estimate the molecular prevalence of natural infection with Sarcocystis spp. in buffaloes using molecular identification. In addition, phylogenetic analyzes were used for the first time to identify the different species of this protozoan infecting buffalo in the south of the country. Heart samples from 80 buffaloes were subjected to microscopic examination, followed by molecular analysis. Microcysts were present in 19/80 (23,75%) of the samples. Genomic DNA was extracted from the 19 isolates, all there were amplified DNA in the primer used in the study. Six readable sequences were obtained after sequencing of the samples in both the directions. In the present study all the sequenced samples indicated were of Sarcocystis levinei.

scholarly journals Molecular Characterization of Sarcocystis Species Isolated from Sheep and Goats in Riyadh, Saudi Arabia

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 256 ◽  
Author(s):  
Dina M. Metwally ◽  
Mashael A. Al-Damigh ◽  
Isra M. Al-Turaiki ◽  
Manal F. El-Khadragy
Keyword(s):  
Saudi Arabia ◽  
Protozoan Parasite ◽  
Final Host ◽  
Molecular Testing ◽  
Sarcocystis Species ◽  
Sheep And Goats ◽  
Transmission Electron ◽  
First Time ◽  
Intracellular Protozoan Parasite

Sarcocystosis is induced by species of Sarcocystis, which is an intracellular protozoan parasite in the phylum Apicomplexa. The diversity and importance of Sarcocystis species in sheep and goats in Saudi Arabia are poorly understood. In this study, the tongue, esophagus, heart, diaphragm, and skeletal muscles were collected from 230 sheep and 84 goats, and the tissues were examined for the presence of Sarcocystis species by macroscopic examination and light microscopy. Microscopic Sarcocystis species cysts were found in both sheep and goats. Transmission electron microscopy (TEM) revealed S. tenella in sheep and S. capracanis in goats. Sarcocystis species were confirmed for the first time in Saudi Arabian sheep and goats by molecular testing. S. capracanis was most closely related to S. tenella, with the COX1 sequences sharing 91.7% identity. A phylogenetic analysis produced similar results and indicated that the Sarcocystis isolates were within a group of Sarcocystis species in which dogs were the final host. Finally, the Sarcocystis species cysts from sheep and goats could be grouped together, indicating that they were strongly related.

scholarly journals Significance of Neospora caninum in cattle farming

2006 ◽  
Vol 60 (3-4) ◽  
pp. 223-231
Author(s):  
Tamara Ilic ◽  
Sanda Dimitrijevic
Keyword(s):  
Neospora Caninum ◽  
Protozoan Parasite ◽  
The United States ◽  
Neonatal Deaths ◽  
Obligate Intracellular ◽  
The World ◽  
Three Stages ◽  
Almost All ◽  
Cycle Covers ◽  
Intracellular Protozoan Parasite

Neospora caninum is an obligate intracellular protozoan parasite which primarily causes diseases in dogs and cattle all over the world. It was first described in Norway in the mid-eighties in dogs, after which, until the present time, clinical neosporosis was proven in sheep, goats, deer, rhinoceroses, horses, and experimental rodents. Antibodies against N. caninum have been found also in the serum of water buffalo, red and gray foxes, coyotes, camels, and felines. Due to the similarity of this Coccidia with Toxoplasma gondi, the neosporosis was for a series of years incorrectly diagnozed as toxoplasmosis. Domestic canines, dogs, are the only real host for N. caninum. Its life cycle covers three stages of development: tachyzoites, tissue cysts and oocysts. Carnivores are infected by ingesting parts of infected tissue which contain tissue cysts with bradyzoites. The dominant pathway of transmission of this cause in cattle is transplacentary infection, but cattle can also be infected by ingestion of feed or water contaminated by sporulated oo-cysts of N. caninum. Bitches can be subclinical carriers of the parasite, when they pass on the cause transplacentarily, which results in more than one litter being born with the infection. Neosporosis today appears as the main cause of abortions and neonatal deaths in dairy cows and fattening cattle in almost all parts of the world, but with the highest incidence in the United States (US), New Zealand, The Netherlands, and Germany. The treatment of this disease has not been fully determined, but medicines used for the treatment of toxoplasmosis have yielded certain good results. There is no verified vaccine that would prevent undesired abortions in cattle. .

Electron microscopic observation of cytoskeletal frame structures and detection of tubulin on the apical region of Cryptosporidium parvum sporozoites

Parasitology ◽  
2007 ◽  
Vol 135 (3) ◽  
pp. 295-301 ◽  
Author(s):  
M. MATSUBAYASHI ◽  
H. TAKASE ◽  
I. KIMATA ◽  
H. NAKAGAWA ◽  
H. TANI ◽  
...  
Keyword(s):  
Cryptosporidium Parvum ◽  
Electron Microscopic Observation ◽  
Protozoan Parasite ◽  
Apical Region ◽  
Electron Microscopic ◽  
Apicomplexan Parasites ◽  
Cytoskeletal Structure ◽  
The World ◽  
Tubulin Antibodies ◽  
Intracellular Protozoan Parasite

SUMMARYCryptosporidium parvum is an intracellular protozoan parasite belonging to the phylum Apicomplexa, and a major cause of waterborne gastroenteritis throughout the world. Invasive zoites of apicomplexan parasites, including C. parvum, are thought to have characteristic organelles on the apical apex; however, compared with other parasites, the cytoskeletal ultrastructure of C. parvum zoites is poorly understood. Thus, in the present study, we ultrastructurally examined C. parvum sporozoites using electron microscopy to clarify the framework of invasive stages. Consequently, at the apical end of sporozoites, 3 apical rings and an electron-dense collar were seen. Two thick central microtubules were seen further inside sporozoites and extended to the posterior region. Using anti-α and -β tubulin antibodies generated from sea urchin and rat brain, both antibodies cross-reacted at the apical region of sporozoites in immunofluorescent morphology. The molecular mass of C. parvum α tubulin antigen was 50 kDa by Western blotting and the observed apical cytoskeletal structures were shown to be composed of α tubulin by immunoelectron microscopy. These results suggested that C. parvum sporozoites were clearly different in their cytoskeletal structure from those of other apicomplexan parasites.

Molecular-Based Identification of Sarcocystis hominis in Belgian Minced Beef

2007 ◽  
Vol 70 (6) ◽  
pp. 1523-1526 ◽  
Author(s):  
L. VANGEEL ◽  
K. HOUF ◽  
K. CHIERS ◽  
J. VERCRUYSSE ◽  
K. D'HERDE ◽  
...  
Keyword(s):  
18S Rrna ◽  
Intestinal Tract ◽  
Protozoan Parasite ◽  
Sarcocystis Species ◽  
Rrna Sequence ◽  
Specific Sequence ◽  
Sarcocystis Spp ◽  
Minced Beef ◽  
Pcr Products ◽  
Human Intestinal Tract

Sarcocystis hominis, one of the three species of Sarcocystis that cause muscular cysts in cattle, is a protozoan parasite that can infect the human intestinal tract. The objective of the present study was to develop a new molecular identification method capable of discriminating among the bovine Sarcocystis species and to apply this tool in combination with stereo-microscopy to determine the presence of Sarcocystis spp. in minced beef in Belgium, with special attention to Sarcocystis hominis. A PCR technique based on the 18S rRNA sequence and by sequencing of the amplicon was highly specific. Sequence analysis of PCR products from thick-walled cysts collected from minced beef in Belgium revealed that S. hominis was present in 97.4% of the samples. Because the consumption of raw minced beef is common in Belgium and certain other European countries, these findings may point to an underestimated risk to public health.

A New Host and Locality Record for Mesocestoides sp. Tetrathyridia (Cestoidea: Cyclophyllidea), with a Summary of the Genus from Snakes of the World

1991 ◽  
Vol 77 (2) ◽  
pp. 329 ◽  
Author(s):  
Chris T. McAllister ◽  
David Bruce Conn ◽  
Paul S. Freed ◽  
Deborah A. Burdick
Keyword(s):  
Locality Record ◽  
New Host ◽  
The World

Temperature, Mitochondria, and Reye's Syndrome

PEDIATRICS ◽  
1983 ◽  
Vol 71 (6) ◽  
pp. 985-985
Author(s):  
RIF S. EL-MALLAKH
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Protozoan Parasite ◽  
Mitochondrial Enzyme ◽  
Reye's Syndrome ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Mitochondrial Defect ◽  
Intracellular Protozoan Parasite

To the Editor.— Mitochondrial failure, manifest by changes in mitochondrial enzyme activity1-3 and morphology,4-5 is central to Reye's syndrome (RS).6 Although it has been variously hypothesized that the mitochondrial changes are secondary to an exogenous toxin,7-12 or an intrinsic mitochondrial defect,6 the actual cause remains obscure. Electron microscopic studies have shown sweelling and loss of cristate in mitochondria of patients with RS. It is interesting that very similar changes occur in Trypanosoma cruzi.13-16 T cruzi is an extracellular/intracellular protozoan parasite which causes Chagas' disease.17

scholarly journals First record of Gryllophila skrjabini Sergiev, 1923 and Cephalobellus magalhaesi Schwenk, 1926 (Nematoda: Thelastomatidae) parasites of Neocurtilla claraziana Saussure, 1874 (Orthoptera: Gryllotalpidae) in Argentina

2017 ◽  
Vol 57 (37) ◽  
pp. 473
Author(s):  
José Matias Rusconi ◽  
Maria Fernanda Achinelly ◽  
Nora Beatriz Camino
Keyword(s):  
Buenos Aires ◽  
Field Survey ◽  
Host Record ◽  
Nematode Species ◽  
First Record ◽  
Agricultural Pests ◽  
New Host ◽  
New Host Record ◽  
The World ◽  
First Time

Thelastomatidae is one of the largest families parasitizing insects, within the order Oxyurida. In this work we reported parasitism in nymphs and adults of Neocurtilla claraziana by two different thelastomatid species as a part of a field survey on agricultural pests. Nymphs and adults of this insect were isolated from grasslands of Buenos Aires State, Argentina using a tensio-active solution. The nematode species Gryllophila skrjabini Sergiev, 1923 and Cephalobellus magalhaesi Schwenk, 1926 are briefly described and measurements are given. Both nematodes are reported for the first time in Argentina with C. magalhaesi being the second isolation of this species in the world. Neocurtilla clarziana is a new host record for G. skrjabini.

scholarly journals Possible adaptation measures of agriculture sector in the Nile Delta to climate change impacts

2009 ◽  
Vol 3 (1) ◽  
pp. 123-126 ◽  
Author(s):  
S. M. Attaher ◽  
M. A. Medany ◽  
A. F. Abou-Hadid
Keyword(s):  
Climate Change ◽  
Nile Delta ◽  
Climate Change Impacts ◽  
Yield Reduction ◽  
Agricultural System ◽  
Delta Region ◽  
Adaptation Measures ◽  
Agriculture Sector ◽  
The World ◽  
Impacts Of Climate Change

Abstract. The overall agricultural system in the Nile Delta region is considered as one of the highest intensive and complicated agriculture systems in the world. According to the recent studies, the Nile Delta region is one of the highly vulnerable regions in the world to climate change. Sea level rise, soil and water degradation, undiversified crop-pattern, yield reduction, pests and disease severity, and irrigation and drainage management were the main key factors that increased vulnerability of the agriculture sector in that region. The main objective of this study is to conduct a community-based multi-criteria adaptation assessment in the Nile Delta using a preset questionnaire. A list of possible adaptation measures for agriculture sector was evaluated. The results indicated that the Nile Delta growers have strong perceptions to act positively to reduce the impacts of climate change. They reflected the need to improve the their adaptive capacity based on clear scientific message with adequate governmental support to coop with the negative impacts of climate change.

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